In order to explain the ecological-sociological strategy of Tehran land use network/ land cover, the present study was tried to identify the spatial process of the change in the landscape mosaic pattern and its effect on carbon sequestration over the course of 3 decades from 1990 to 2020. In this study, the role of landscape measures in the identification of landscape mosaic spatial pattern changes and its combination with data obtained from ecosystem service modeling was shown. To this end, after examining the process of structural-functional changes in landscape mosaic using decision tree algorithm and InVEST model, the complexity of the relationship between landscape spatial pattern and the quantity of carbon sequestration regulatory services at the two levels of class and landscape was addressed using the measures and data obtained from InVEST model. The findings indicated that the volume of carbon sequestration regulatory services in all periods had reduced due to the effects of the spatial process of “accumulation” in construction strains. Moreover, in the 1990-2020 period, the highest rate of carbon storage loss (291656 tones, 9.58%) occurred due to the effects of the spatial processes of “deletion” in green lands and “fragmentation” in barren lands. In the 2000-2010 and 2010-2020 periods, the volume of carbon storage reduction were 182209 (6.62%) and 159688 (6.22%) tones, respectively. Pertaining to this discussion was the finding that the effect of the change in the index of strain numbers in green and barren lands for all periods was accompanied by increased fineness, which indicates its reverse relationship with carbon sequestration rate. On the other hand, the reduction of other measures in green and barren lands reveals the existence of a direct relationship with the loss of a volume of carbon sequestration in the foregoing period. At the landscape level, the reduction of heterogeneity and simplification of the landscape mosaic spatial pattern led to the reduction of carbon sequestration. From this perspective, it is necessary to prioritize the identification of the spatial processes effective on the landscape structure model in the completion of the processes of the evaluation of the urban development effects on the carbon sequestration service current.