To meet the demand for an appropriate soaking duration and priming type, there is a need to embrace cheap, fast, natural, accessible, and adaptable physiological techniques as soaking of seeds in water and fresh cow milk. Fresh cow milk contains hormones that relieve seeds from photo, thermo, physiological and mechanical dormancy. In light of this, this study was conducted to assess the effect of hydration and fresh cow milk on different seed quality attributes like germination percentage, mean germination time, the uncertainty of germination process, and synchrony of germination process of bitter gourd seeds. The study involved two factors namely hydropriming and different concentrations of fresh cow milk (viz. 60%, 80% and 100%), and the other factor was seed soaking duration with 4 levels (i. e., 0, 12, 18 and 24 hours). The experiment was conducted in a Complete Randomized Design (CRD) with three replicates. Results revealed that the percentage germination value of seeds soaked in all concentrations of fresh cow milk was better than hydropriming. A significant germination percentage value of 96% and 93% were recorded for seeds treated for 18 hours in 80% and 100% concentrations of fresh cow milk respectively. Maximum uncertainty of the germination process and minimum synchrony of germination the process was recorded in untreated seed.

A study using the assigned Complete Block Design with three replications was carried out at Hobigong, Bangladesh from January to August 2021 to investigate the impact of different combinations of organic and inorganic fertilizers on the growth and production of bitter gourd. The experiment employed five different combinations of organic and inorganic fertilizers, which are listed below: T1: 4 kg of organic fertilizer per plant, T2: 4 kg of organic fertilizer + 2 liters of cow urine + 2 liters of bc (biochar), T3: 2 liters of liquid bc (biochar)) + NPK (44 g urea + 43 g TSP (Triple Super Phosphate) + 77 g MoP (Muriate of Potash), T4: NPK (44 g urea + 43 g TSP + 77 g MoP), and T5: 4 kg of organic fertilizer + 2 liters of bc+ NPK (44 g urea + 43 g TSP + 77 g MoP) on three distinct soils in the villages of Kalonjhora, Madhabpur, and Jointure. The growth and yield of bitter gourd at different study locations were significantly affected by the application of both organic and inorganic fertilizers, according to the results. The results showed that the use of both organic and inorganic fertilizers had a significant effect on the development and yield of bitter gourd at the different study sites. Maximum germination rate (90%), at 45 DAS (days after sowing), longest plant (173.28 cm) at 100 DAS, maximum number, longest and heaviest fruit (6.30 number of fruits per plant, 15.51 cm and 82.50 g, respectively) were obtained from crops grown in Jointer village that were applied with 4 kg organic fertilizer + 2 liters bc + NPK (44 g urea + 43 g TSP + 77 g MOP). According to the previously described research, most development and yield characteristics of bitter gourd are improved when grown in a mixture treated with 4 kg organic fertilizer + 2 liters bc + NPK (44 g urea) + 43 g of TSP + 77 g of MoP.