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Management of Erwinia amylovora by Potential Bio-Pesticides in vitro and in vivo Conditions
2020
Kubilay Kurtulus Bastas
Erwinia amylovora, the causative agent of fire blight disease, threatens a lot of species of the Rosaceae family. Antibiotics and copper compounds in chemical applications are most frequently are applied, but these can be phytotoxic and cause resistant strains of the pathogen. In our experiments, 20 herbal materials were tested for their antimicrobial effectiveness against the fire blight pathogen in vitro and in planta. The air-dried plants ground into fine powder and extraction was performed at room temperature by maceration with 80% (v/v) methanol/distilled water. The minimum inhibitory concentration values were determined by using disc diffusion method and streptomycin was used as control in all experiments. Antimicrobial activity was evaluated by measuring the inhibition zones in reference to the pathogen. Among the tested plants, Szygium aromaticum, Thymus vulgaris and Rhus cararia showed a good antibacterial activity and they inhibited the growth of E. amylovora with inhibition zone diameter ranging from 21 to 27 mm at 20% (w/v) in absolute methanol compared to streptomycin (31 mm) in vitro conditions. In vivo tests were performed by using highly virulent E. amylovora isolate (Eak24b, 91%) grown on TSA medium and inoculation on young shoots of 3-year-old Gala variety of apple and Santa Maria variety of pear seedlings at 107 CFU ml-1 density of the pathogen. Disease severity (%) was assessed by by proportion of blighted shoot length to the whole shoot length and also efficacy of the extracts was determined by using Abbott formula. The highest efficacy was obtained by S. aromaticum and T. vulgaris extracts of reducing shoot blight of cv. Gala and cv. Santa Maria by 67.81% - 64-12% and 51.50% - 51.04% ratios, respectively. Obtaining results showed that some medicinal and aromatic plant extracts might be used against fire blight disease as potential new generation chemicals on pome fruits within integrated and organic control programs.
Show more [+] Less [-]Effectiveness of Phosphorous acid, Bacillus subtilis and Copper Compounds on Apple cv. Gala with M9 Rootstock in the Control of Fire Blight
2023
Ayşegül Gür | Kubilay Kurtuluş Baştaş
Necrogenic Gram-negative bacterium Erwinia amylovora, which causes economic losses especially in pome fruits such as apple, pear, quince and loquat, some berries and many ornamental plants, causes fire blight disease. Copper compounds are used extensively in disease control programs and they can cause toxicproblems in terms of plant and environmental health. In addition, the formation of resistance to copper in the pathogen is frequently observed. In this study, plant activator phosphorous acid and biological control agent Bacillus subtilis, and 3 different copper compounds, Copper sulfate, Copper oxychloride and Copper hydroxide which are commonly used against E. amylovora, on apple cv. Gala with M9 rootstock were evaluated comparatively. When the new season shoot lengths of 3-year-old plants with homogeneous growth reached 20-25 cm, chemicals and B. subtilis were applied first time before one week ago from the pathogen inoculation, and after 2 times with 1 week intervals. The youngest two leaves at tips of actively growing terminal plant shoots were inoculated by cutting off using scissors dipped in suspension of E. amylovora str. EaARADY5 containing 108 CFU ml-1. Disease assessments were made after the disease severity (%) was determined on the basis of shoot blight after symptom development of the disease stopped, and the results were found to be statistically significant (p<0.05). According to the findings, while the highest effect was obtained by copper oxychloride with 69% effectiveness among all applications, the lowest effectiveness was obtained with 43.5% copper sulfate. It has been revealed that the use of the most effective of the copper compounds in field applications will result in less exposure to chemicals in terms of human and environmental health, and that B. subtilis and phosphorous acid can be used significantly in the integrated control of fire blight.
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