This study was carried out in Tokat Gaziosmanpaşa University, Faculty of Agriculture, Field Crops Department, Industrial Plants Tissue Culture Laboratory in 2020. In the study, effects of different media (LS media, LS+1 mg/L Gibberellic acid (GA3), 0.1 mg/L kinetin (KIN) and LS+0.5 mg/L indole-3-acetic acid (IAA) on sweet potato genotypes (Havuc and Hatay Yerlisi) were investigated. Plant and root weights, plant and root lengths, and number of nodes and roots were determined. In this study, plants grown in cytokinin and gibberellin media had higher root number, root weight, root length, plant height, number of nodes and plant weight compared to the ones grown in auxin media. Havuc local genotype had higher values in terms of the investigated traits in all of the media studied.

The present study was conducted to find out an in vitro efficient method for multiple shoot regeneration of two local chickpea varieties. The mature embryos were excised of two chickpea varieties i.e. Bittle-98 and Dasht-2000 (with cotyledon and without cotyledon) used as explants. The explants were cultured on Murashige and Skoog (MS) medium supplemented with three concentrations of (2, 3, 4 mg/l) 6-benzylaminopurine (BAP) using explant with and without cotyledon. Further, 0.5 mg/l α-naphthaleneacetic acid (NAA) along with varied concentrations of BAP (2, 3, 4 mg/ l) was also tested using explant with cotyledon. 3 mg/l BAP alone and 3 mg/l BAP with 0.5 mg/l NAA were found the most effective cytokinin in multiple shoot induction in both tested varieties. Bittle-98 and Dasht-2000 showed 82% and 76% elongation in shoots induction with 0.2 mg/l Indole-3-acetic acid (IAA). Root formation was recorded 80% and 60% in Bittle-98 and Dasht-2000 with 1.0 mg/l Indole-3-butyric acid (IBA). Whereas, recorded root formation was 40 and 20% in Bittle-98 and Dasht-2000 with 1.0 mg/l NAA. The best response for rooting observed in Bittle-98 as its roots were thick, long and strong. Plantlets of Bittle-98 were acclimatized in solid medium for 7-14 days. The successful invitro regeneration of Bittle-98 was observed, when excised embryo with cotyledon were used as explant, made it valuable for genetic transformation.