Refine search
Results 1-9 of 9
Effect of CuO Nanoparticle on Ceratophyllum demersum
2022
Hayder Alhamadani | Muhittin Dogan
In this study, it was aimed to determine some physiological effects of CuO nanoparticle on C. demersum. The plants were collected from an uncontaminated pond in Gaziantep province. Different concentrations of CuO (0, 25, 50, 100 and 200 mg/L) were applied to the macrophytes after being acclimatized in controlled conditions. Some analyses were made on macrophytes harvested at the end of the application. The contents of protein, total carbohydrate, photosynthetic pigment, total phenolic compound, non-protein sulfhydryl groups of tissues increased with increasing CuO concentration under the influence of applied CuO concentrations. In addition, increases in hydrogen peroxide and MDA contents were also detected. As a result, it was determined that the applied CuO concentrations caused some physiological changes in C. demersum tissues.
Show more [+] Less [-]Examination of Relationships Between Some Biochemical and Oxidative Stress Traits by Canonical Correlation Analysis in Broiler Chickens
2018
Sıddık Keskin | Emine Berberoğlu | Şenay Sarıca
Canonical correlation analysis is a multivariate method to examine the relationships between two (X and Y) sets of variables when all measurements are obtained from same broilers. Canonical correlation analysis aims to obtain new variables called as canonical variates formed by linear combinations of the original variables for each set and by maximizing the relationships between two set. The purpose of this study is to examine the relationships between 8 biochemical traits (Aspartate Aminotransferase (AST), Albumin, Triglyceride, Total Cholesterol, Low Density Lipoprotein (LDL) cholesterol, Glucose, Total Protein and Alanine Aminotransferase (ALT)) and 4 oxidative stress traits (total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), lipid peroxide (LPO)) in broiler chickens. As a result, the correlation between the first canonical variable pair was found 0.594.
Show more [+] Less [-]Effect of Polyamine on Pigmentation, Reactive Oxidative Species and Antioxidant under Drought in Maize (Zea mays L.)
2018
Sharmin Akter | Md. Golam Rasul | Mohammad Zakaria | Md. Mahathir Sarker | Irin Sultana Nila | Sudipta Dutta | Md. Masudul Haque | Md. Motiar Rohman
To examine polyamines (PAs) effect in modulating the drought induced by polyethylene glycol (PEG) in maize (Zea mays L.) seedlings (variety Khoibhutta, 8 day seedlings grown in petri dish in incubator) were subjected to 20% PEG (polyethylene glycol) followed by 20 µmol PAs, Putrescine (Put), Spermidine (Spd) and Spermine (Spm) with PEG solution for 48 hours. Sharp decrease in Relative Water Content (RWC), Chl a, Chl b, carotenoid (Car) and total pigment content was observed under drought compared to control condition, while PAs application reversed their decreasing trends. PEG significantly increased Reactive Oxidative Species (ROS) [superoxide (O2•−) and H2O2], Methyl Glyoxal (MG), Melondialdehyde (MDA) and Lipoxigenase (LOX) activity, while Pas decreased the contents considerably (except MG) as compared to those under drought. Drought increased proline content, which was further augmented in PA treatments. PAs failed to incline glyoxalase’s (Gly-I and Gly-II) activities, reduced under PEG. The activity and western blot confirmed the accumulation of Glutathione S-Transferase (GST) under drought, but PAs failed to augment the activity. Ascorbic Acid (AsA) and Glutathione (GSH) got oxidized into Dehydroascorbate (DHA) and oxidized Glutathione (GSSG) under drought but PAs effectively maintained homeostasis. Superoxide Dismutase (SOD), Peroxidase (POD), Ascorbate Peroxidase (APX), Glutathione Peroxidase (GPX), Monodehydroascorbatereductase (MDHAR), Dehydroascorbatereductase (DHAR), and Glutathione Reductase (GR) inclined in drought stressed seedlings, while Catalase (CAT) activity decreased under drought. PAs addition increased SOD, POD, GPX, CAT, MDHAR, and GR activities, but declined DHAR activity. These findings suggested important role of PAs in increasing tolerance under short term drought by modulating antioxidant effect.
Show more [+] Less [-]Determination of Malondialdehyde (MDA), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) Levels in Kangal Dogs with Maternal Cannibalism
2017
Nazlı Ercan | Mustafa Koçkaya
Oxidative stress, which plays an active role in the pathogenesis of various diseases, continues to be relevant in assessing the condition changes such as aggression. Some of Kangal dogs have been found to eat their own puppies during their first 24 hours following birth, as a case called maternal cannibalism. The present study aims to determine the levels of serum malondialdehyde (MDA), which is a product of lipid peroxidation, and serum glutathione peroxidase (GPx) and superoxide dismutase (SOD) enzymes, which are the parameters of antioxidant defense system, and total protein and albumin levels and to show their relationship with cannibalism. The study material consists of blood and blood serum of 30 Kangal dog breed in total, 15 of which have maternal cannibalism and 15 haven’t observed maternal cannibalism as a control group. Glutathione peroxidase, superoxide dismutase, malondialdehyde levels were investigated by means of spectrophotometric method in blood serum of these animals. The difference between the mean values of total protein, GPx, SOD, MDA and albumin was statistically significant in the results obtained. These findings conclude that oxidative stress and antioxidative metabolism plays a role in the pathogenesis of cannibalism in dogs.
Show more [+] Less [-]Phytochemical Profile and Antioxidant Activities of Aqueous Extract of Moringa oleifera (Lam) Collected from DR Congo and Kenya
2022
Valence Bwana Mutwedu | Albert Wafula Nyongesa | Jafred Mulama Kitaa | Jemima Achieng Oduma | James Mucunu Mbaria
Moringa oleifera Lam. is one of the most used plants in traditional medicine because of its high antioxidant properties. The antioxidant value, nonetheless, depends on locality where the plant is grown as well as specific parts on the plant. In this study, a phytochemical and antioxidant activity comparison of M. oleifera leaves, seeds and barks were carried out. Fresh leaves, seeds and barks were collected from 2 to 3 years old M. oleifera trees of Bukavu city of South Kivu province in DRC and Masii village of Machackos County in Kenya. A total of 300g of each dried sample powder was mixed with 700 mL of distilled water. Qualitative and quantitative assessment of alkaloids, saponins, phenols, flavonoids, glycosides, terpenoids and tannins were performed following standard methods while the antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results indicate that only alkaloids were absent in leaves from Kenya and DRC while phenols, flavonoids and tannins were absent in barks. Glycoside in seeds from DRC had the highest concentration (6.17%) followed by alkaloids in seeds from Kenya (5.56%). There was low concentration of terpenoids and flavonoids in all samples compared to other compounds. The highest extract yield was found in leaves from DR Congo (22.5%) and seeds from Kenya (20%). At the highest concentration (10 µg/mL), leaves from Kenya (88.29±1.12 µg/mL) and DRC (80.17±3.59 µg/mL) had the highest percentage inhibition of reactive oxygen-free radicals but lower than the reference standard (92.63±2.76 µg/mL). Leaves from Kenya (23.59 μg/mL) and DRC (28.67 μg/mL) had the highest IC50 compared to mean values of seeds and barks from the two countries. M. oleifera leaves, especially from Kenya, are recommended as a satisfactory antioxidant but can be substituted with seeds and/or barks in order to alleviate the use of leaves which are overused these days.
Show more [+] Less [-]Antioxidant Activity and Element Content of Suillus collinitus
2021
Celal Bal
Mushrooms used as food have medicinal importance due to their antioxidant compounds. In this context, it is very important to determine the biological potential of fungi and to reveal these medicinal properties. In this study, it was aimed to determine the element contents, total antioxidant status, total oxidant status, oxidative stress index of Suillus collinitus (Fr.) Kuntze mushroom. In this context, the mushroom samples were extracted with ethanol in the Soxhlet extractor. Element contents were determined using atomic absorption spectrometry. Total antioxidant (TAS) and total oxidant (TOS) levels and oxidative stress index (OSI) were determined using Rel Assay commercial kits. As a result of the study, it was determined that the TAS value of S. collinitus was 2.467 ± 0.145 mmol/L, TOS value was 17.845 ± 0.273 µmol/L and OSI value was 0.677 ± 0.030. In addition, the Fe content (350.72 ± 10.23), Cu content (68.11 ± 2.51), Pb content (11.58 ± 2.43), Zn content (10.46 ± 1.28) and Ni content (1.47 ± 0.21 mg.kg-1) of S. collinitus measured. As a result, S. collinitus mushroom is thought to be a natural source of antioxidants. It has also been observed that the element contents are at normal levels.
Show more [+] Less [-]The Effect of Enteritis, Pneumonia and Omphalitis on Oxidative/Antioxidant Balance in the Calves
2019
İbrahim Yurdakul | Ugur Aydogdu
In this study, it was aimed to determine the effects of enteritis, pneumonia and omphalitis on oxidative/antioxidant balance in the calves. In total, 27 calves with neonatal disease and 10 healthy calves (control) were used. The sick calves were divided into 3 groups according to localization of infection, omphalitis (n = 10), pneumonia (n = 8) and enteritis (n = 9). Blood samples of the calves were taken from jugular vein for analysis. Blood in tubes without anticoagulant was centrifuged at 5000 rpm for 5 min following clotting, and the sera were removed and stored at -80°C until analyzed. Serum malondialdehyde and nitric oxide levels of all groups were significantly higher, while glutathione, glutathione peroxidase, catalase and superoxide dismutase levels were significantly lower compared to the control group. As a conclusion, diarrhea, pneumonia and omphalitis are caused by oxidative damage in the calves, and antioxidant treatment may be beneficial in the treatment of these diseases.
Show more [+] Less [-]Antioxidant Activity of Quercetin: A Mechanistic Review
2016
Senay Ozgen | Ozgur Kivilcim Kilinc | Zeliha Selamoğlu
Flavones and flavonoids are known to have potent antioxidant activity due to intracellular free radical scavenging capacities. Flavonoids are found ubiquitously in plants as a member of polyphenolic compounds which share diverse chemical structure and properties. Quercetin is among the most efficient antioxidants of the flavonoids. The antioxidant property of quercetin has been highlighted in this review. These compounds have pivotal role in treatment of diabetes, cancers and some cardiovascular diseases.
Show more [+] Less [-]Investigation of the Effect of Pumpkin (Cucurbita pepo L.) Seed Oil on Pentylenetetrazole-induced Neuronal Damage in HT-22 Cell Line
2023
Ahmet Taskiran | Tuğba Yıldız Asdemir
Recent studies have shown the positive effects of Cucurbita pepo L. (pumpkin) seed oil (PSO) in different disease models. However, the effect of PSO on neurological diseases has not been clarified yet. Therefore, this study aims to elucidate the effects of BBS on pentylenetetrazole (PTZ)-induced neuronal damage and the possible roles of oxidative and nitrosative stress in this effect in vitro. The HT-22 hippocampal neuronal cell line was used in the study. Cell survival after PTZ-induced neuronal damage was evaluated with the XTT test in the groups. While the effects of BBS on total antioxidant status (TAS) and total oxidant status (TOS) after PTZ were measured with colorimetric commercial kits, its effects on neuronal nitric oxide synthase (nNOS) and nitric oxide (NO) levels were also determined by ELISA kits. In light of the data obtained, it was found that pre-treatment with PSO prevented the decrease in cell survival after exposure to PTZ. In addition, it has been found that PSO normalizes the increase in TOS, nNOS, and NO in neuronal cells after PTZ. As a result, it was determined that the treatment of neuronal cells with PSO prevented neuronal damage caused by PTZ and showed neuroprotective properties. It is thought that PSO may achieve these effects through oxidative and nitrosative systems. Enrichment of a daily diet with PSO might be beneficial in reducing the risks of neurological diseases.
Show more [+] Less [-]