Honey, bee venom, royal jelly, pollen, propolis and other bee products are natural products that are used to protect and maintain human health, to create a strong and dynamic health condition, and used as a ethnopharmacological medicine in the therapy of some illness. The use of different bee products to strengthen the immune system is still very common today. In this study, it was aimed to determine the in vitro effects of royal jelly samples collected from Sivas province in HUVEC cell culture conditions by determining the healing endothelial damage. In this context, royal jelly samples were obtained from the province of Sivas, where beekeeping is carried out intensively and successfully, in the 2022 harvest period. The effect of royal jelly extracts on the cell proliferation of endothelial cell culture was investigated. Repair of endothelial cell damage, cell growth and development and migration values were measured using the HUVEC cell line, and the data were evaluated with SPSS, GraphPad, ImageJ programs. Although there were significantly important increases in cell growth, development and cell viability values in HUVEC cells treated with different doses of royal jelly at the end of 24 and 48 hours, no statistically significant difference was found in terms of cell migration ratio compared to the control group.