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The Effects of Royal Jelly Samples Collected from Sivas Province on the Proliferation of Endothelial Cells Full text
2023
Safa Uğur Koçköprü | Sevgi Durna Daştan
Honey, bee venom, royal jelly, pollen, propolis and other bee products are natural products that are used to protect and maintain human health, to create a strong and dynamic health condition, and used as a ethnopharmacological medicine in the therapy of some illness. The use of different bee products to strengthen the immune system is still very common today. In this study, it was aimed to determine the in vitro effects of royal jelly samples collected from Sivas province in HUVEC cell culture conditions by determining the healing endothelial damage. In this context, royal jelly samples were obtained from the province of Sivas, where beekeeping is carried out intensively and successfully, in the 2022 harvest period. The effect of royal jelly extracts on the cell proliferation of endothelial cell culture was investigated. Repair of endothelial cell damage, cell growth and development and migration values were measured using the HUVEC cell line, and the data were evaluated with SPSS, GraphPad, ImageJ programs. Although there were significantly important increases in cell growth, development and cell viability values in HUVEC cells treated with different doses of royal jelly at the end of 24 and 48 hours, no statistically significant difference was found in terms of cell migration ratio compared to the control group.
Show more [+] Less [-]The Effects of Royal Jelly Samples Collected from Sivas Province on the Proliferation of Endothelial Cells Full text
2023
Safa Uğur Koçköprü | Sevgi Durna Daştan
The Effects of Royal Jelly Samples Collected from Sivas Province on the Proliferation of Endothelial Cells Full text
2023
Safa Uğur Koçköprü | Sevgi Durna Daştan
Honey, bee venom, royal jelly, pollen, propolis and other bee products are natural products that are used to protect and maintain human health, to create a strong and dynamic health condition, and used as a ethnopharmacological medicine in the therapy of some illness. The use of different bee products to strengthen the immune system is still very common today. In this study, it was aimed to determine the in vitro effects of royal jelly samples collected from Sivas province in HUVEC cell culture conditions by determining the healing endothelial damage. In this context, royal jelly samples were obtained from the province of Sivas, where beekeeping is carried out intensively and successfully, in the 2022 harvest period. The effect of royal jelly extracts on the cell proliferation of endothelial cell culture was investigated. Repair of endothelial cell damage, cell growth and development and migration values were measured using the HUVEC cell line, and the data were evaluated with SPSS, GraphPad, ImageJ programs. Although there were significantly important increases in cell growth, development and cell viability values in HUVEC cells treated with different doses of royal jelly at the end of 24 and 48 hours, no statistically significant difference was found in terms of cell migration ratio compared to the control group.
Show more [+] Less [-]Estudo do processo de extração de papaína a partir do látex do fruto de mamão (Carica papaya L.) cv. Maradol Full text
2011
Martínez Correa Hugo A. | Morales Rodríguez Olga | Andrade Mahecha Margarita M
Neste trabalho foi estudado o processo de extração da papaína presente no látex de frutos de mamão (<em>Carica papaya </em>L<em>.</em>) cultivar Maradol. As variáveis estudadas na extração da papaína foram proporção de látex:álcool (1:2.1 e 1:3) e tipo de secagem (à vácuo e por refractance window). As respostas obtidas foram atividade enzimática da enzima e rendimento do processo de extração. O melhor resultado em termos de atividade enzimática e rendimento foi obtido nas condições de secagem à vácuo e proporção látex:álcool de 1:3. A enzima obtida foi caracterizada por testes físico-químicos, microbiológicos e de atividade enzimática e comparada com uma amostra comercial usada como padrão.
Show more [+] Less [-]Estudo do processo de extração de papaína a partir do látex do fruto de mamão (Carica papaya L.) cv. Maradol Full text
2011
Andrade-Mahecha, Margarita M(Universidade Estadual de Campinas -UNICAMP Faculdade de Engenharia de Alimentos) | Morales-Rodríguez, Olga(Universidad del Tolima Facultad de Ingeniería Agronómica) | Martínez-Correa, Hugo A(Universidad Nacional de Colombia sede Palmira Facultad de Ingeniería y Administración)
Neste trabalho foi estudado o processo de extração da papaína presente no látex de frutos de mamão (Carica papaya L.) cultivar Maradol. As variáveis estudadas na extração da papaína foram proporção de látex:álcool (1:2.1 e 1:3) e tipo de secagem (à vácuo e por refractance window). As respostas obtidas foram atividade enzimática da enzima e rendimento do processo de extração. O melhor resultado em termos de atividade enzimática e rendimento foi obtido nas condições de secagem à vácuo e proporção látex:álcool de 1:3. A enzima obtida foi caracterizada por testes físico-químicos, microbiológicos e de atividade enzimática e comparada com uma amostra comercial usada como padrão. | In this work, we studied the Papain extraction process, from the latex of papaya fruit (Carica papaya L.) cv. Maradol. The variables studied in the extraction of papain were: the proportion of latex:alcohol (1:2.1 and 1:3) and type of drying( vacuum and refractance window) and the responses obtained in terms of enzymatic activity of the enzyme papain and yield of the extraction process. The best result in terms of enzyme activity and yield was the procedure used to vacuum drying and the proportion latex:alcohol 1:3. The enzyme obtained was characterized by physico-chemical, microbiological and enzymatic activity when compared with a commercial sample used as standard.
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