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Water Deficit Tolerance of Some Pepper Inbred Lines
2019
Davut Keleş | Hasan Pınar | Atilla Ata | Mustafa Bircan | Zeki Karipçin | Ufuk Rastgeldi | Saadet Büyükalaca
Water deficit is one of the main limiting factors affecting plant growth. Selection in water-limited environments can result in populations or species with improved response to drought. Water deficit decreases yield and quality, therefore, it is important to identify genotypes that are tolerant to deficit irrigation conditions. In this study, the water-deficit tolerance of 59 pepper-inbred lines was determined. Experiments were conducted in a growth chamber and under field conditions (Şanlıurfa) with a control (100% full-irrigation) and water-deficit treatment (50% irrigation). Fruit weight, fruit length and number of fruits were recorded. Pepper lines 1900, 896 A-W, 74, 760, 1560-W, 912 A-W, 405-A, 953-W, 226, 1105-W and 441 were identified as the most tolerant to water deficit conditions. Present findings revealed that these pepper lines could be used to develop cultivars that have satisfactory yield under water deficit conditions.
Show more [+] Less [-]Effects of Gamma Irradiation on Seedlessness and Fruit Quality of Ortanique Tangor
2020
Berken Çimen | Turgut Yeşiloğlu | Yıldız Aka Kaçar
The existence of a large amount of seeds in citrus fruits results as a major impediment to customer acceptability, even if the fruits have high organoleptic properties. Irradiation, which mainly reduces seed number of varieties, is a faster way than hybridization. The use of irradiation in citrus breeding programs is now quite widespread with most programs in the major citrus producing countries actively developing new selections. The present study reports the primarily results of gamma irradiation on seed number and fruit quality of Ortanique tangor mutant population. The shoots of scion were irradiated with gamma rays from 60Co at the dose of 50 Gy (gray) in April of 2014. All the treated buds were then immediately budded onto rootstocks and the survival rate was recorded as 60.34%. In order to stabilize the mutation, mV3 plants were developed by re-budding and plants at mV3 generation were transplanted in the orchard in 2017. Within the following year, 68 mV3 plants out of 852 grown in the field bore sufficient amount of fruit and were screened in terms of 19 fruit quality characters such as seed number per fruit, fruit diameter and ripening index. Fruit diameters of mV3 population varied from 56.72 mm to 84.79 mm, and fruit weight ranged between 90.00 g and 287.60 g. The number of seeds per fruit ranged between 0.6 and 13.1 whereas seed number of non-irradiated Ortanique tangor was recorded as 10.7 in the same fruit crop year. In general, fruit characteristics such as fruit weight, fruit height and fruit diameter which describe fruit size of a big part of the population, were similar to Ortanique tangor. According to primarily results, 18 plants have been described as low seeded (≤4). In addition, a cluster analysis was performed by using Euclidean similarity coefficient and similarity index ranged between 29.29% and 93.10% regarding variables related to fruit. The stability of mutations detected is being evaluated and new commercial field trials will be established with the selected materials.
Show more [+] Less [-]Comparison of Some Carcass Components of Selected Japanese Quail Lines in terms of SNP Haplotypes
2019
Kemal Karabağ | Emel Tüten Sevim | Taki Karslı | Sezai Alkan
In this study, we investigated the effect of SNP haplotypes on insulin-like growth factor gene (IGF-1) related with weights of body, back, chest, leg and wing in the fifteen generation selected Japanese quail (C. coturnix japonica) lines according to the 5th week live weight. 8 SNP haplotypes were identified in the 167-bp DNA sequence of the IGF-1 gene coding region identified in a total of 108 individuals from three quail lines (control, high body weight: HBW and low body weight: LBW). ANOM (Analysis of Means of Analysis) was performed to determine the relationships between carcass components with SNP haplotypes and to compare quail genotypes. There were significant differences between the quail lines in terms of SNP haplotypes. As a result, it was determined that fifteen-generation selection in Japanese quails resulted in differences in insulin-like growth factor-1 gene and these differences were reflected in carcass components.
Show more [+] Less [-]Response of Some Pepper Genotypes to Cucumber Mosaic Virus (CMV) and Discrimination of Kilis Isolates Using High-Resolution Melting (HRM) Method
2024
Melisa Balcı | Bekir Bülent Arpacı | Mehmet Koç | Ümit Haydar Erol
The escalating global population, diminishing agricultural lands, and the overarching global climate crisis are significantly impacting pepper cultivation. These challenges exacerbate the vulnerability of pepper plants to various biotic and abiotic factors, particularly viral diseases, resulting in diminished yield and quality. Cucumber mosaic virus (CMV), a notable concern for peppers in the Solanaceae family, is causing substantial quality and yield losses, with no effective chemical control methods currently available. This study focuses on exploring the genetic structure of CMV isolates obtained from pepper production areas in Kilis province and comparing these regions through the High-Resolution Melting (HRM) method. CMV isolates, cultivated in tobacco plants, have their partial coat protein sequences compared with those of other CMV isolates registered in the gene bank. The nucleotide sequences of identified CMV isolates are phylogenetically grouped and compared using an HRM graph. The HRM graph effectively distinguishes Kilis 3 and Kilis 4 isolates, clustered similarly to sequence patterns, from other isolates. The study highlights the utility of HRM analyses in identifying differences between isolates before determining sequence patterns. In the gene bank comparison, Kilis CMV isolates distinguished from others. Similarities were observed with isolates from Iran's Balsam (Impatiens balsamina - LC066478), Türkiye's Radish (Raphanus sativus - LC0665051), and Wild Turnip (Rapistrum rugosum - LC066514, LC066511, LC066517). The study found that Kilis 7 CMV isolate, transferred mechanically to 24 different pepper genotypes (C. annuum) from the local population, revealed susceptibility to CMV in the 24 lines developed from the Elbeyli population in Kilis province.
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