Defining new genetic markers to understand the population structure and genetic basis of cattle breeds, increase production and improve yield quality is of great importance in the field of modern breeding technology. In this study, it was aimed to identify new molecular marker polymorphisms by using DNA sequence analysis method on the Insulin-Like Growth Factor-Binding Protein, Acıd-Labile Subunit (IGFALS) gene, which affects different yield characteristics in cattle. 20 samples from PCR products with various base sizes were randomly chosen for sequence analysis. The study revealed that the IGFALS gene’s first exon region, which is 492 bp long, contains the g.1368491 A/G polymorphism. A 463 bp length region of the second exon was where the g.1369854 -/G insertion was found.

Determination of genetic resources and their diversity constitutes are the initial step of the breeding programs. Before starting to the selection of the desired properties, many molecular methods are used to determine the existing genetic potential and determine the genotypic values. Many molecular methods (RAPD, RFLP, AFLP, SSR and DNA sequencing etc.) based on PCR technology are used to identify populations, identify genes related to yield and resistance, and demonstrate phylogenetic relationships. Many software’s for the analysis of molecular datasets obtained from molecular methods have been developed and the field of bioinformatics was born. In this study, after DNA sequence analysis; it is aimed to give information about haplotype analysis by determining SNPs (Single Nucleotide Polymorphism's) in individuals of populations, calculation of the genetic distance among populations and within populations, and software and analysis used for phylogenetic tree drawing.