Honey is known to be a natural food product with antibacterial and wound-healing properties. Among its different types, some attributed more with medical applications including Manuka honey. In this study, some wound healing-related parameters were systematically investigated on two different types of Turkish honey (Chestnut and Anzer), in comparison with Manuka honey. Chestnut honey had considerable amount of hydrogen peroxide (5 μg/mL) whereas Manuka and Anzer honey did not contain any. Additionally, Chestnut honey had the highest acidity among all tested samples (24.50 meq/kg), as well as higher antioxidant activity (IC50 ; 2.45 mg/ml) than those of Anzer (13.02 mg/ml) and Manuka honey (6.27 mg/ml). It was also demonstrated that Chestnut honey displayed a considerable antimicrobial activity against selected pathogens. On the other hand, in the scratch assay performed as a model for the wound healing process, Anzer honey induced the highest cell proliferation corresponding to almost total wound closure for all concentrations tested. Overall, the results showed that the Turkish Chestnut and Anzer honey samples had distinguished characteristics for wound healing that is comparable to Manuka honey, and therefore are both promising candidates for use in clinical applications.