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Isolation of Alpha-amylase Producing Thermophilic Bacillus Strains and Partial Characterization of the Enzymes
2015
Celal Türker | Bahri Devrim Özcan
In the present study, we isolated three thermophilic Bacillus strains from the soil samples collected from the coast sediments of the Burnaz Stream located in Erzin. The isolates were entitled as Bacillus sp. CT1, CT2, and CT3, respectively. The maximum α-amylase production was revealed at 60°C for CT1 strain, and at 80°C for CT2 and CT3 strains, respectively. The optimum enzyme activity was observed at 90°C for CT1 α-amylase, whereas at 60°C for CT2 and CT3 α-amylases. On the other hand, optimum pH value for CT2 α-amylase was 7.0, whereas 8.0 for CT1 and CT3 α-amylases. The specific activities of CT1, CT2, and CT3 amylases were 317.6, 113.3 and 362.7 U/mg at 55°C, respectively. The estimated molecular weight of CT1 and CT3 α-amylase was 65 kDa, and for CT2 α-amylase was 38 kDa by zymogram analysis.
Show more [+] Less [-]The relationships between phenylthiocarbamide taste perception and smoking, work out habits and susceptibility to depression
2015
Sevgi Durna Daştan | Yusuf Muhammed Durna | Taner Daştan
Phenylthiocarbamide (PTC) is known as phenylthiourea and it is an organic compound that has the phenyl ring. Ability to perceive the tastes of PTC chemical is related to the dominance of taste genes. There are a large number of population studies regarding the PTC taste perception and different personal characteristics or disease conditions. The purpose of this study is to reveal and compare the relation between the PTC taste perception and work-out habits, smoking, alcohol consumption and tendency to the depression of people. A total of 2500 adults were volunteered to be included in this study. PTC taste perception was measured by tasting with PTC solution (10 mg/L) filtered in a paper. It showed that tasters were significantly more frequent (81.8%) than nontasters (18.2%) in all population. And in some parameters analyzed in this study, there are significant differences. The taste genetics show up with environmental factors and create the sense of taste, which develops the feeding behaviors. The taste perception resulting from food and beverages diversifies by genetic and environmental effects and the nervous system interprets this perception. This study is enlightening in terms of presenting that the taste perception of people affects their lifestyles and lead them to start and either continue or discontinue some habits.
Show more [+] Less [-]Evaluation of Different Groundnut Arachis hypogea L. Cultivars Against Termites, Odontotermes obesus (Rambur) in Rawalpindi, Pakistan
2015
Muhammad Usman Malik | Humayun Javed | Mahmood Ayyaz
Six groundnut (Arachis hypogea L.) cultivars i.e. Golden, BARI-2000, Australian, BARD-479, ICG-7326 and 2KCGC20 were evaluated against the attack of Odontotermes obesus in terms of percentage infestation of workers and soldiers, their damage and impact on the yield. The maximum mean populations of O. obesus workers were recorded on 2KCGC20 (30.82±2.75 workers/plot) and Australian (30.65±2.92 workers/plot), followed by BARI 2000 (19.55±2.51 workers/plot), Golden (17.30±2.27 workers/plot) and BARD-479 (16.82±2.06 workers/plot). The minimum mean population of O. obesus workers was found on ICG-7326 cultivars (14.85±1.81) workers/plot. The maximum mean population infestation of O. obesus soldiers was recorded on 2KCGC20 cultivar (4.88±0.25 soldiers/plot) followed by Australian (4.15±.30, soldiers/per plot), BARD-479 (2.00±.17 soldiers/plot), ICG-7326 (1.70±.14 soldiers/plot), BARD 2000 (2.40±.25 soldiers/ plot). The minimum mean population of O. obesus soldiers was found on Gold (1.87±.17 soldiers/plot). Population of O. obesus workers was positively related with the damage and negatively related the yield in all the groundnut cultivars.
Show more [+] Less [-]The Aggregation of Boron on the Tissues of Gold Fish (Carassius auratus Linnaeus, 1758)
2015
Tuncer Okan Genç | Burak Evren İnanan | Murat Yabanlı | Fevzi Yılmaz
In this study, it was aimed to determine the water-borne and food-borne boron accumulation in the liver and muscle tissues of Gold Fish (Carassius auratus Linnaeus, 1758). For each treatment, 12 individuals were. The water-borne boron treatments were applied as boron acid concentration of 1 mg/L, 10 mg/L and 20 mg/L in the aquarium water, while the food-borne boron treatments were prepared food contained the defined levels of boron (1 mg/kg, 5 mg/kg and 10 mg/kg) as boric acid. The boron levels in the tissues were determined by an ICP-MS procedure. The maximum boron concentration was found in the 20mg/L water borne boron treatment in the liver tissue (1.78±0.02 mg/kg). In the water-borne boron treatments, the maximum Transfer Factor (TF) was found in the 20mg/L boron concentration, and TF values were increased when the boron concentrations were decreasing. In the 1 mg/kg food-borne boron treatment, TF was found as 0, and increasing concentration of boron in the food caused an increase in TF reached about 0.06. This study suggested that the target organ for boron accumulation is the liver rather than the muscles and the accumulation of food-borne boron is lower when comparing water-borne boron.
Show more [+] Less [-]The Symptoms of Herbicidal Action: The Case of Aclonifen
2015
Özgür Kıvılcım Kılınç
The symptoms which were directly bound to the biochemical mode of action of an herbicide, other symptoms result from an indirect consequence of this action. The symptoms of herbicidal action deeply differed and that the climatic factors during the two first weeks after treatment could change definitely the result of the selective herbicide action. The repetitive observation of symptoms allows to inform the farmer about the tolerance or resistance of certain plant species, including the culture, for instance through the appearance of symptoms on the first leaves of the seedlings and their absence in the following leaves. As a whole, the accurate observation of herbicidal symptoms on plants is the essential, rapid and non-expensive analysis of treatment effectiveness at the field scale. The purpose of the current report is to describe the symptoms of a very complex herbicidal action, that of aclonifen involving two modes of action for the same molecule, approximately at the same concentration, and to compare these symptoms under field conditions and under controlled conditions, for a better understanding.
Show more [+] Less [-]Epipelic Diatoms as Indicators of Water Quality in the Lower Part of River Melet (Ordu, Türkiye)
2015
Beyhan Taş | Özlem Yılmaz | Işıl Kurt
Phytobenthos includes bioindicator species and is widely used in water ecology studies. Diatoms constitute one of the most important groups of phytobenthos in streams. In particular, these organisms are good indicators in investigations related with determining of water quality in medium and long time. In this study, the epipelic diatom flora of the lower part of River Melet were investigated, the most important source of drinking water in Ordu city. The examination was performed periodically in March-November 2012 and total of 56 taxa were identified. Cymbellales (14 taxa) and Naviculales (16 taxa) ordo constituted 54% of diatom diversity. These were followed by Fragilariales (16%, 9 taxa) Bacillariales (14%, 8 taxa) Surirellales (9%, 5 taxa) Achnanthales (3%, 2 taxa) Eunotiales (2%, 1 taxa) and Melosirales (2%, 1 taxa), respectively. Diatome vulgaris, Melosira varians, Navicula gregaria, N. tripunctata and Nitzschia sigmoidea species were recorded as widespread and intense in the epipelic communities. These species are usually tolerant to organic pollution and are found in β-α- mesosaprobic conditions. According to the obtained results, the lower part of the Melet River has character from pollution towards moderate pollution. In other words, it has II-III. class water quality.
Show more [+] Less [-]The Effects of Different Treatments on Phytochemical and Volatiles Compositions of ‘Hicaznar’ pomegranate Fruit During Cold Storage
2015
Ayşe Tülin Öz | Ebru Kafkas | Mozhganx Zarifikhosroshahi | Tülin Şahin
‘Hicaznar’ pomegranate fruits were stored under both modified atmosphere packaging (MAP) and normal atmosphere without packaging at +7oC temperature and % 85-90 RH for 18 weeks. Weight loss, % CO2/O2 gas changes, arils color changes [L (lightness) and C (chroma)] values, total soluble solids (TSS) content, pH, titratable acidity (TA), total phenolic content (mg/100mL), total flavonoid content (mg/100mL), antioxidant activity (%), malic acid (%), changes of sugar (glucose, fructose, saccarose and sorbitol) content and volatile aroma compounds of ‘Hicaznar’ pomegranate fruits were determined during cold storage. Both average weight loss of pomegranate fruits and aril L (lighness) colour value changes were increased while aril C (Chroma) colour changes was decrease slightly at the end of storage. Total phenolic content in control arils samples were determined 109.5 mg/100 mL while it was 127.8 mg/100 mL in MAP aril samples; also total flavonoid content was decreased in 26.8 (control) and 27.1 (MAP) respectively at the end of storage compared to begining. The decrease of TSS was statistically significantly different between treatments. Moreover in this study volatile profile of ‘Hicaznar’ pomegranate fruit was desribed and 27 volatile compounds identified using automatic headspace solid-phase microextraction gas chromatography -mass spectrometry (HS-SPME GC/MS) tecniques. ‘Hicaznar’ pomegranate fruits have alcohols, aldehide, alkan, acids, esters, terpene and another volatile. Both effects of storage type and duration were evaluated. The results showed that terpenes increased for both MAP and control fruit samples while alcohols, aldehide and acids were decreased for both of storage type at the end of storage.
Show more [+] Less [-]A simple method for diagnostic of Phytophthora infestans (Mont.) de Bary from potato agricultural fields of potato
2015
Touseef Hussain | Bir Pal Singh | Firoz Anwar | Sonica Tomar
A correct detection and appropriate identification of causal pathogens associated with crop plants or seeds are considered to be the most important issue in designing the proper management plans for plant diseases. This study was designed to detect Phytophthora infestans inoculum from potato grown soil. A high detection rate of P.infestans was obtained from the naturally infested soil of potato fields. Naturally soils were firstly moistened in a plastic pots and then pre-incubated at ±18°C for 3 days, baiting with potato tuber slice for 24, 48, and 72 h. The baits were then thoroughly washed, flooded with 10–15 ml of distilled water in Petri-dishes and incubated under continuous darkness in chamber ±18ºC. Sporangia started to emerge from the margins of potato tuber slice. They were easily observed under the stereomicroscope. Pure culture of the fungus was obtained by isolating from baited tubers on a Rye Agar medium. This is the first report of recovery of P. infestans from naturally infested potato growing soils using susceptible potato tuber (K. Bahar) as bait in India. All isolates were determined to be A2 mating type.
Show more [+] Less [-]Production of Whey Powder Added Fruit Beverages and Some Quality Characteristics
2015
Büşra Ece Argan | Onur Güneşer | Ayşegül Kırca Toklucu | Yonca Karagül Yüceer
In this study, functional fruit beverages were produced by mixing whey with fruit concentrates (apple and cherry) or pulp (apricot) at certain amounts, and some physical, chemical and sensory characteristics of the beverages were revealed during storage. While the highest total phenol content and antioxidant capacity (TEAC) were determined in beverages with cherry juice, TEAC values of the products changed based on fruit types used in the beverages during storage. In addition, it was determined that fruit type had significant effect on acidity, invert sugar, total sugar and total phenol of beverages. Beverage with apricot pulp was the most liked product by consumers.
Show more [+] Less [-]Relationship of Broodstock Weight and Hatching Yield of III. Generation (F3) Black Sea Trout (Salmo trutta labrax Pallas, 1814)
2015
Hakan Baki | Eyüp Çakmak | Birol Baki | Cemil Altundaş
In this study, this was the aimed to determine the reproduction and hatching yields in different weight groups in hatchery origin rootstocks (F3) Black Sea trout (Salmo trutta labrax Pallas, 1814). In this study, the female fishes which produced from natural rootstock were used average weight 1437±134.6 g (Group I) 2737±210.2 g (Group II) and 3785±162.3 g (Group III). After the stripping process; according to the groups, were determined respectively eggs absolute fecundity (number of eggs/broodfish) is average 2353±205, 5361±506, 6603±491, relative fecundity (number of eggs/kg)) is average1687±166, 1968±194, 1744±114. The egg diameter (mm) 4.89±0.16, 5.28±0.07, 5.31±0.06 egg weight (mg) 77±6, 90±4, 96±3 groups I., II. and III. respectively was calculated. The fertilization ratio (%) 95.49±1.23, 96.49±1.14, 98.39±0.52, browse rate (%) 79.97±5.36, 84.20±5.23, 94.70±1.50, has been identified. The output rate (%) 60.57±7.86, 67.66±6.42, 84.83±3.09, the hatchery efficiency (%) 58.14±7.95, 69.77±6.25, 83.51±3.29 was found. relative fecundity average value 168±160, 1968±194, 1744±114.The existence of relationship among the weight of the brood-stock, absolute fecundity (0.0198, 0.0012, 0.3499), and relative egg fecundity (0.3415, 0.0494, 0.00) was tested by regression analysis and consequently the difference between groups were significant. This was determined that between fish weight and absolute fecundity relationship is weak directly proportional for each group, between fish weight and relative fecundity is weak inversely proportional in Group I and II, the Group III is weak for directly proportional.
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