This study presents a comprehensive investigation into the interactions of two distinct synthetic dyes—1,9-Dimethyl-Methylene Blue and Toluidine Blue —with different DNA structures, namely calf thymus DNA (ct-DNA), G-quadruplex DNA, and single stranded DNA (ssDNA). The objective of this research is to elucidate the molecular affinities of these dyes for specific DNA structures and explore their potential applications in molecular biology and diagnostics. The experimental approach involved detailed UV-visible spectroscopic analyses, to probe the binding affinities of each dye with ct-DNA, G-quadruplex DNA, and ssDNA. The study aimed to assess the selectivity of these dyes towards the unique structural features of each DNA entity. The binding stoichiometry is defined from Job’s method. The selectivity of the dyes towards DNA also investigated with competitive dialysis experiments. The binding stoichiometry were 1:1 for 1,9-Dimethyl-Methylene Blue and Toluidine Blue and G-quadruplex DNA or ssDNA. Besides, results indicate that 1,9-Dimethyl-Methylene Blue and Toluidine Blue exhibit a pronounced affinity for G-quadruplex DNA, and ct-DNA. While single-stranded DNA is a fundamental component of DNA replication and transcription, our dyes exhibit lower affinity for this structure. The selectivity is advantageous, as it allows for the discrimination between single-stranded and structured DNA regions. The potential applications in studying DNA dynamics and unwinding processes are vast.

Root-knot nematodes (RKN), (Meloidogyne spp.), are the major biotic factor responsible for the limiting production of Kiwifruit in Nepal including Kiwifruit orchard of Warm Temperate Horticulture Center, Nepal. Hence, there is a pressing demand for nematicides that are both easily accessible and cost-effective while being environmentally friendly. A screenhouse experiment was conducted in the Summer of 2023 with an objective to evaluate the effects of different plant extracts, bio-control agents, and chemical nematicides against RKN on Kiwifruit seedlings. The experiment was set up in a Completely Randomized Design with three replications and eight treatments which include the extracts of Allium sativum and Lantana camara, Trichoderma viride, Pseudomonas fluorescens, Cartap hydrochloride, Fosthiazate, Inoculated control and Uninoculated control. The results revealed that Trichoderma viride proved to be the most effective in reducing the nematode population, displaying a low root gall index of 3.11, a minimal reproductive factor of 0.24, and a high percentage of nematode control at 91.71%. It was also found to be efficient in promoting the growth parameters of Kiwifruit seedlings. Additionally, regression analysis exhibited a significantly positive interaction between root gall index and reproductive factor, while indicating a negative interaction between reproductive factor and growth parameters. Therefore, T. viride (@ 20 gm per 2000 cm3 of soil) should be soil drenched before the seedlings are transplanted into the main field for effective and sustainable management of RKN. Nevertheless, further research is needed to determine the efficacy of T. viride in infested roots of Kiwifruit trees in field condition of Kiwifruit orchard.

Conversion of land use from forest to agricultural uses modifies soil quality through physiochemical soil properties changes. This study was conducted in Rwanda’s central plateau agro-ecological zone to evaluate the effect of forest and agricultural land uses on soil quality. The study was conducted in 2020. Soil samples were collected at the top, middle and bottom positions of each of the two land uses. We analyzed soil bulk density, soil moisture content, soil pH, soil organic matter (SOM), total nitrogen (TN), available phosphorus (Av P), and CEC for each position of the land uses. Data were analyzed using ANOVA in GENSTAT version 13. The results revealed that soil properties were significantly affected by land use change. Analysis of variances (LSD<0.05) results showed, however, that treatments were not significantly different within the same land use. The results showed that treatments from top position of forest lands had the highest mean values for soil organic matter and total N parameters with the respective mean values of 6.58 %, and 0.37 %. Treatments from middle position of forest lands had the highest mean values for soil moisture content and Av P parameters respectively with 23.60 % and 29.56 ppm. But, soil bulk density was high on top position of agricultural land with a mean value of 1.49 g/cm3. Land users are advised to apply crop and soil management techniques which maintain soil quality and productivity on agricultural lands.

In the research conducted under the conditions of Tokat, silage triticale-vetch mixture-second crop silage corn rotation was applied. The study used four different tillage methods to compare the quality and product yield of the seedbed prepared for silage triticale-vetch mixture. Conventional tillage method (M1), conservation tillage method (M2), reduced tillage method (M3), and direct sowing (M4) methods were applied. Seedbed quality: It was evaluated regarding soil moisture content, bulk density, penetration resistance, degree of soil fragmentation, and surface roughness for depths of 0-10 cm and 10-20 cm. The effect of soil tillage methods on porosity, surface roughness, and green grass yield were statistically insignificant. Although there were statistical differences between the methods regarding soil moisture content (MC), bulk density (BD), penetration resistance (PR), and mean weight diameter values (MWD), the values are within the limit values determined for plant growth. However, crop yield is the same between soil tillage methods. This result shows that alternative tillage methods are applicable when evaluated in sustainable agriculture, which does not create a statistically significant difference in crop yield compared to conventional tillage.

Potatoes for use in industrial processing must have a low reducing sugar concentration. This study investigates the impact of organic and synthetic fertilizers on the composition of Irish potato cultivars’ reducing sugar, specific gravity, dry matter, and starch. The research was conducted at Busogo Farm in Rwanda, using randomized complete block designs in seasons 2021 B and 2022 A with rate of ten tones per hectare under the doses of 100%, 50%, and 0% and three hundred kilograms per hector under the dose of 100%, 50% and 0% respectively for farmyard manure and NPK 17.17.17. Results showed significant effects of season and treatment on reducing sugar levels in potatoes. Twihaze and Kirundo varieties recorded high glucose (0.09%), while Gisubizo variety in control, recorded low glucose (0.01%). Additionally, the analysis of variance showed that Irish potato varieties with low to moderate and moderate to high glucose contents, respectively, experienced extremely significant (p 0.001) effects of farmyard manure and NPK on glucose content for the seasons 2021 B and 2022 A. The application of inorganic and organic fertilizers had a significant impact (p 0.05) on dry matter in seasons B and A, according to the analysis of variance. Additionally, the results demonstrated extremely significant (p<0.01) differences in specific gravity and starch in both seasons, with Kinigi variety having higher values of dry matter, specific gravity, and starch content in season B and A, respectively, of 24.58, 1.10, and 18.59%. Season 2022 A’s high decreasing sugar levels were caused by meteorological conditions brought on by the soil’s high moisture content as well as the maturity of the tubers. Due to its low level of reducing sugar, Gikungu, Kirundo, and Kinigi are excellent for chips whereas Twihaze variety is advised for boiling cooking methods.

This study was conducted to determine the effect of rearing quails in different rearing systems on egg production, egg quality characteristics, and incubation results. The 360 quails were distributed equally to 3 different rearing groups (cage, enriched cage, and floor) in a male:female mixed at the age of 3 weeks. The eggs were individually weighed and recorded one by one daily (5487 eggs) for 3 months. To determine egg quality and incubation characteristics, 750 eggs were broken, and 3284 eggs were incubated, respectively. The results showed that female quail in the floor group have lower body weight at 6 weeks old (177.19 g), and they reached sexual maturity (age of first egg laying) later (65.36 day, P<0.01), The lowest egg production (61.14%) occurred in the floor group during the 3-month egg production period (P<0.05). The lowest average egg weight (9.07 g) was determined in the floor group (P<0.05). It was determined that the eggs of quails raised in the enriched group had a rounder shape index because they have larger widths (P<0.01). While the highest average yolk height (11.24 mm) was determined in the enriched cage group (P<0.01), the highest averages of albumen length (72.41 mm) and lowest averages of yolk index (48.43%) were determined in the cage group (P<0.01). The lowest average values (113.17) in Haugh unit were detected in the floor group (P<0.05). The highest fertility rate (98.44%) was in Floor♂:Cage♀, the highest hatchability of fertile egg rate (94.67%) was obtained from Enriched♂:Cage♀, and the highest hatchability (89.10%) was obtained from Floor♂:Cage♀ pairing (P<0.05). The lowest fertility rate (88.00%) was obtained from the Enriched♂:Floor♀, the lowest hatchability of fertile egg rate (86.01%) was obtained from the Enriched♂:Enriched♀, and the lowest hatchability (75.62%) was obtained from the Enriched♂: Enriched♀ groups. As a result, it has been revealed that the effect of various rearing systems is different on egg production, egg quality traits, and hatching results in quails. Thus, it has been shown that different programs at the rearing period to be applied to the male and female quails can achieve better incubation results and reproductive success.

Excessive sugar intake can lead to poor health outcomes. The use of sweeteners is considered as a strategy to reduce added sugar consumption. The presence of sweeteners in food products has increased significantly in many countries. Therefore, the aim of this study was to identify the added sugars and sweeteners in packaged products. 1550 (57.9%) out of 2676 food products contained at least one added sugar. Confectionery and desserts (35.38±24.82 g/100 g), and snack foods (20.70±16.20 g/100 g) were the main categories containing the highest amount of added sugar. 229 (8.6%) out of 2676 food products contained various types of sweeteners. The most popular added sugar was sucrose (62.9%), while the most popular sweetener was sorbitol (28.2%). This is the most comprehensive study in the city center of Edirne province in Türkiye reporting on the types and frequency of added sugars and sweeteners in packaged products. This study offers monitoring to improve the legislation of Türkiye on added sugars and sweeteners used in the food supply.

A study using the assigned Complete Block Design with three replications was carried out at Hobigong, Bangladesh from January to August 2021 to investigate the impact of different combinations of organic and inorganic fertilizers on the growth and production of bitter gourd. The experiment employed five different combinations of organic and inorganic fertilizers, which are listed below: T1: 4 kg of organic fertilizer per plant, T2: 4 kg of organic fertilizer + 2 liters of cow urine + 2 liters of bc (biochar), T3: 2 liters of liquid bc (biochar)) + NPK (44 g urea + 43 g TSP (Triple Super Phosphate) + 77 g MoP (Muriate of Potash), T4: NPK (44 g urea + 43 g TSP + 77 g MoP), and T5: 4 kg of organic fertilizer + 2 liters of bc+ NPK (44 g urea + 43 g TSP + 77 g MoP) on three distinct soils in the villages of Kalonjhora, Madhabpur, and Jointure. The growth and yield of bitter gourd at different study locations were significantly affected by the application of both organic and inorganic fertilizers, according to the results. The results showed that the use of both organic and inorganic fertilizers had a significant effect on the development and yield of bitter gourd at the different study sites. Maximum germination rate (90%), at 45 DAS (days after sowing), longest plant (173.28 cm) at 100 DAS, maximum number, longest and heaviest fruit (6.30 number of fruits per plant, 15.51 cm and 82.50 g, respectively) were obtained from crops grown in Jointer village that were applied with 4 kg organic fertilizer + 2 liters bc + NPK (44 g urea + 43 g TSP + 77 g MOP). According to the previously described research, most development and yield characteristics of bitter gourd are improved when grown in a mixture treated with 4 kg organic fertilizer + 2 liters bc + NPK (44 g urea) + 43 g of TSP + 77 g of MoP.

The main goal of this study was to assess the qualitative composition of essential oil (P), a natural extract produced from wasted orange peels in the citrus processing sector. Additionally, the study aimed to investigate the alterations in fish feeds when this essential oil is incorporated and stored. The essential oil was extracted from the orange peels provided for the study using the hydrodistillation method and the Clevenger apparatus. The study determined the volatile components of the oil with 100% accuracy. The primary constituent was identified as D-limonene, comprising 59.27% of the total composition. Subsequently, experimental feed groups were established by including the essential oil in the feed rations at ratios of ‰0 (P0), ‰0.5 (P5), ‰1 (P10), and ‰3 (P30). Under storage conditions, the feeds were stored in feed sacks for 60 days. Periodic samples were collected during storage and subjected to nutritional, microbiological, structural, and oxidation tests. The results of the study show that P had no protective effect in fish diets against the growth of yeasts, molds, and other mesophilic aerobic bacteria (p > 0.05). It was shown that the nutritional values varied over time during storage. However, this variation was not substantially correlated with the amount of P in the diets (p > 0.05). There was no discernible impact of the addition of P on the structural characteristics of the feed grains. Nevertheless, the inclusion of P substantially impeded the process of lipid oxidation in the diet (p<0.05). After the two-month storage period, it was shown that adding at least 1 ‰ of P to the fish meals prevented the peroxide generated from oxidation in the feed from exceeding acceptable levels.

Tomato buds of cv. Idkawy were cultured in vitro on solid MS medium with 0.2 mg-l BAP. The plantlets that were produced were exposed to different doses of gamma radiation, ranging from 100 to 200 Gy. Afterward, single pieces of nodes were cut and moved to a fresh MS medium with 0.2 mg-l of BAP. The gamma radiation caused a mortality rate of 18.75% to 52.5% among the explants. The surviving plantlets were then cut into single node pieces and transferred to an MS medium containing 0.2 mg-l of BAP, with added NaCl concentrations of either 50 or 100 mM. There was increased mortality of the vegetative buds on the explants with increased salt concentrations. It was shown that the all gamma radiation doses caused reduced the percentage of survival at saline levels. The genetic diversity was assessment by using ten primers for each SCoT and ISSR markers to six irradiated treatments grew under salt stress (100 Gy x 50 mM, 150 Gy x 50 mM, 200 Gy x 50 mM, 100 Gy x 100 mM, 150 Gy x 100 mM, 200 Gy x 100 mM). It was showed that the polymorphism percentage mean of SCoT marker (29.56%) is higher than the ISSR marker (26.78%). The average of PIC values for both markers SCoT and ISSR were 0.197 and 0.288 (PIC ˂0.5), as well as, MI values were 0.077 and 0.081, respectively. In contrast, when considering the number of alleles (Ne), Nei's genetic diversity (H), and Shannon's information index (I) parameters, it was observed that the greatest genetic variation was caused by the combined treatment of 200 Gy x 50 mM NaCl using the SCoT marker. On the other hand, with the ISSR marker, the highest induced genetic variation was seen with the combined treatment of 150 Gy x 50 mM NaCl. The obtained results demonstrate that SCoT marker was more accurate and efficient than ISSR marker for distinguishing and genetic variation analysis of irradiated tomato plantlets grew under salt stress. The relationships within treatments were estimated through cluster analysis (UPGMA) based on SCoT and ISSR analysis.