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The Effects of Humic Acid Applications on Heavy Metal Stress in Lettuce Full text
2016
Fatma Özkay | Sevinç Kıran | Şebnem Kuşvuran | Şeküre Şebnem Ellialtıoğlu
In this study was carried out to determine the effects of humic acid in heavy metal stress of lettuce changes in some of the morphological and physiological characteristics. In studies conducted in controlled greenhouse conditions, lettuce plants subjected to four different 4 different humic acid levels humic acid doses (0, 2, 4, 8 L/da) and 4 different heavy metal irrigation levels (Control: 0 ppm; I. mixture: 0.2 ppm Cu + 0.01 ppm Cd + 5 ppm Pb + 2 ppm Zn; II. mixture: : 0.4 ppm Cu + 0.02 ppm Cd + 10 ppm Pb + 4 ppm Zn; III. mixture: 0.8 ppm Cu + 0.04 ppm Cd + 20 ppm Pb + 8 ppm Zn). Four weeks after at heavy metal and humic acid treatment young plants were harvested and the shoot fresh and dry weight, root fresh and dry weight, shoot and root length, and leaf areas, MDA, superoxyde dismutase (SOD) and glutathione reductase (GR) were determined. The most highly toxic effects were determinate III. mixture level (0.8 ppm Cu + 0.04 ppm Cd + 20 ppm Pb + 8 ppm Zn). MDA and antioxidative enzyme activities increased in plants irrigated with water containing a mixture of heavy metal. Humic acid applications had a positive effect on reducing of the limiting effect of heavy metal stress on growth and development.
Show more [+] Less [-]Effects of the Hydrolysable Oak Tannins on the Quail Performance Full text
2016
Mesut Karaman | Trefa Kamal M. Azize | Mehmet Sait Ekinci
In the present study effects of different levels of hydrolyzed oak tannin on Japanese quail, live body weight and live body weight gain, food intake, food conversion efficiency and mortality rate were investigated. Therefore, a total of 240 quails were weighed and divided into 4 treatment groups each with 3 replicates containing 20 birds and reared in flat for 42 days as experimental period. While one of the groups was fed control diet with no additives (0% tannin A), other groups were fed with the different percentages of tannin on their diets, which are 0.5% (B), 1.0% (C), 1.5% (D) respectively. It has been observed that Japanese quails that fed the oak tannin containing diet had slower growth rate and poorer feed conversion efficiency and high mortality rate than birds fed with a tannin-free diet.
Show more [+] Less [-]Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species Full text
2016
Muhammad Amjad Nawaz | Faheem Shehzad Baloch | Hafiz Mamoon Rehman | Bao Le | Fahima Akther | Seung Hwan Yang | Chung Gyuhwa
Extraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.
Show more [+] Less [-]Determination of Total Costs, Labour Requirements and Work Efficiencies in Second Fodder Corn Silage Production at Bafra Town of Samsun Full text
2016
Taner Yıldız
This study was completed with the aim of determining work efficiencies, labour requirement, and total cost of second fodder corn silage agricultural enterprises on flat ground in Bafra district of Samsun province. According to the results, total variable and fixed costs were calculated 2827.80 TL ha-1 and 4224.60 TL ha-1. The shares of variable and fixed costs in total production costs were determined 40.10% and 59.90%, respectively. Land hire had 24.70% as the highest share in fixed costs while the highest share for variable costs were observed in fertilization (10.30%) and spraying (7.00%). The highest and the lowest unit of labour requirements (units of human labour power, h ha–1) were obtained for harvesting processes (4.28 h ha-1) and spraying (2.35 h ha-1), respectively. In terms of work efficiencies, the best value was determined (0.53 ha h-1) for the transporting processes.
Show more [+] Less [-]Effect of Storage Time on Nutrient Composition and Quality Parameters of Corn Silage Full text
2016
Betül Zehra Sarıçiçek | Birgül Yıldırım | Zahide Kocabaş | Emel Ozgumus Demir
This study was carried out to determine the effects of storage duration on nutrient composition and silage quality parameters. Corn was used as silage material. Corn (31.41% dry matter) was harvested at the dough stage and fermented for 90, 104, 118, 132, 146, 160, 174, 188 and 202 days in three trench silos. The samples were brought to laboratory every 14 days. This process was repeated 9 times. After the 132th day, whereas silage crude protein (CP), ether extract (EE), ash and crude fiber (CF) contents decreased, nitrogen free extract (NFE) content increased. Lactic acid concentration of corn silage increased until the 118th day but decreased between the 118th and the 160th days. On the contrary, of decrease in LA concentration, acetic acid concentration increased depending on storage time. Ammonia nitrogen and CO2 concentration of silage increased decreased with progressing time. Storage time had significant influence on Flieg scores. The lowest score was found between days the 104th-118th. In this research, it was observed that there was a change in silage nutrient contents and fermentation characteristics with increasing storage time.
Show more [+] Less [-]Larvicidal Activity of Essential Oils from the Leaves and Fruits of Nutmeg (Myristica fragrans Houtt) Against Aedes aegypti (Diptera: Culicidae) Full text
2016
Anne Carolina | Maman Maman
The aim of this research was to examine the larvicidal activity of essential oil (EO) extracted from nutmeg (Myristica fragrans Houtt) leaves and fruits by steam distillation, and to analyze its chemical compounds. The EO yield of nutmeg leaves and fruits collected from the same tree was 0.66% and 0.30%, respectively. Larvicidal tests with the EO were carried out against Aedes aegypti (L.) (Diptera: Culicidae). The concentrations of nutmeg EO used for the larvicidal assay were 50, 100, 150, 200, and 250 μg/mL. The results showed that fruit oil was more toxic than the leaf oil. LC50 values of leaf and fruit EOs were 133.8 and 110.1 µg/mL, respectively. The chromatogram of GC-MS showed that the chemical components in nutmeg leaf and fruit EOs were dominated by α-pinene, sabinene, β-pinene, delta-3-carene, limonene, β-phellandrene, α-terpinolene, linalool, safrole, croweacin, and myristicin.
Show more [+] Less [-]Determination of Antibiotics Resistance Levels in Enterobacteriaceae Isolated from Giresun Coasts Full text
2016
Tamer Akkan | Cengiz Mutlu
In this study the resistance of 200 Enterobacteriaceae isolates recovered from seawater in Giresun Coasts (Black Sea) to 9 different antibiotics was investigated by agar diffusion methods. Antibiotic resistance levels of isolates was determined respectively, Erythromycin (E): 82%, Cefazolin (CZ): 46.50%, Cefotaxime (CTX): 50.50%, Amikacin (AK): 41.50%, Nalidixic acid (NA): 34.50%, Tetracycline (TE): 30.50%, Chloramphenicol (C): 36.50%, Cefuroxime (CXM): 35.50% and Ampicillin (AM): 15.50%. It was found that 2 isolates resistant to all antibiotics, 5 isolates sensitive and 91% of all isolates multiple antibiotic resistance (MAR) index values were higher than 0.2. It was concluded that bacteriological quality in Giresun coastal area could cause public health problems due to the not provided necessary hygiene and sanitation.
Show more [+] Less [-]Status and Future Outlook of Cultivated Mushroom Sector in Turkey Full text
2016
Erkan Eren | Aysun Pekşen
Mushroom production that started in 1960's in Turkey gained economic value from the 1990's and it started improving as a commercial sector after that time. While Turkey mushroom production was 80 tons in 1973, it increased up to 45.000 tons in 2014. There is a rapid changes and improvement in cultivated mushroom production and consumption in Turkey. The object of the study is to reveal current status of mushroom production in the world and Turkey, and the problems of the mushroom sector in Turkey and the necessary precautions and ways to solve these problems. The data obtained from the inspections of the mushroom enterprises that in mushroom production regions of Turkey, and the secondary data obtained from Food and Agriculture Organization of the United Nations (FAO), Turkish Statistical Institute (TUIK) and Union of Antalya Exporters (AIB) and also national and international publication’ data has been used in this study. In Turkey, Mediterranean and Marmara Regions rank first in mushroom production and consumption with the share of 61.5 and 40%, respectively. It has been determined that big enterprises producing mushroom and compost established in the Central Anatolia in this study. The number of enterprises producing mushroom by using technology increases and 15-20% of the total production are provided by the big enterprises having 2000 m2 and over mushroom production areas. Recent years, there are serious increases in the production of different mushroom species such as especially Pleurotus ostreatus. In addition, precision agriculture applications are widespread with the time. For the continuation of growth of the mushroom sector in Turkey and the solving of the sector problems, there are needs both in making the necessary changes in legislation and to be given priority researches related to mushroom production in university and public research institutions and also to support them.
Show more [+] Less [-]The Effects of Dairy Management on Milk Quality Characteristics Full text
2016
Pylyp Petrov | Yaroslava Zhukova | Demikhov Yuriy
The Effects of Dairy Management on Milk Quality Characteristics Full text
2016
Pylyp Petrov | Yaroslava Zhukova | Demikhov Yuriy
The article focuses on the impact of different dairy management types on quality characteristics of organic and conventional milk. The study was conducted during 9 months (spring-autumn), raw milk samples were collected from organic and conventional dairy farms from two Ukrainian regions. The milk samples were analyzed for dry matter, total protein and non-protein nitrogen, ammonia content and values of stable isotopes 13С/12С were measured in fat and milk protein fractions. The values of dry matter, total protein, true protein and non-protein nitrogen content do not represent a statistically significant effect of farming type. The values of ammonia content, ammonia/non-protein nitrogen and ammonia/total protein ratios were statistically significant and reflected the impact of farming type which is, probably, linked with different protein content in cows’ diet. Conversion of some parameters on the dry matter allows more precise differentiation among types of milk, including statistically significant differences (e.g. total protein and ammonia content). The values of δ13C in fat fraction (-26.00‰) and protein fraction (-22.89‰) of organic milk were statistically significant differed in comparison with conventional milk (-23.14 ‰ and -19.15‰ respectively), due to the high amount of maize in the diet on conventional farm. The values of Δδ13C also were statistically significant: 3.99‰ in conventional milk and 3.11‰ in organic. It was established that conventional milk has a greater range of the different parameter seasonal variations than organic milk.
Show more [+] Less [-]The Effects of Dairy Management on Milk Quality Characteristics Full text
2016
Petrov, Pylyp | Zhukova, Yaroslava | Demikhov, Yuriy
The article focuses on the impact of different dairy management types on quality characteristics of organic and conventional milk. The study was conducted during 9 months (spring-autumn), raw milk samples were collected from organic and conventional dairy farms from two Ukrainian regions. The milk samples were analyzed for dry matter, total protein and non-protein nitrogen, ammonia content and values of stable isotopes 13С/12С were measured in fat and milk protein fractions. The values of dry matter, total protein, true protein and non-protein nitrogen content do not represent a statistically significant effect of farming type. The values of ammonia content, ammonia/non-protein nitrogen and ammonia/total protein ratios were statistically significant and reflected the impact of farming type which is, probably, linked with different protein content in cows’ diet. Conversion of some parameters on the dry matter allows more precise differentiation among types of milk, including statistically significant differences (e.g. total protein and ammonia content). The values of δ13C in fat fraction (-26.00‰) and protein fraction (-22.89‰) of organic milk were statistically significant differed in comparison with conventional milk (-23.14 ‰ and -19.15‰ respectively), due to the high amount of maize in the diet on conventional farm. The values of Δδ13C also were statistically significant:3.99‰ in conventional milk and 3.11‰ in organic. It was established that conventional milk has a greater range of the different parameter seasonal variations than organic milk.
Show more [+] Less [-]Improvement on The Ellis and Roberts Viability Model Full text
2016
Guoyan Zhou | Feifei Zhang | Shaoyun Wu
With data sets of germination percent and storage time of seed lot of wheat and sorghum stored at three different storage temperature(t, °C) with three different water content (m, %) of seeds, together with data set of buckwheat and lettuce reported in literatures, the possibility that seed survival curve were transformed into line by survival proportion and the relationship that logarithm of average viability period (logp50) and standard deviation of seed death distribution in time (δ)with t, m and interaction between t and m were analysed. Result indicated that survival proportion transformed seed survival curve to line were much easier than the probability adopted by Ellis and Roberts, and the most important factor affecting logp50 and δ of seed lot was interaction between t and m. Thus, Ellis and Roberts viability model were suggested to be improved as Ki=Vi-p/10K-CWT (t×m) to predict longevity of seed lot with initial germination percent unknown, a new model of Gi/G0=A-P/10K-CWT(t×m) was constructed to predict longevity of seed lot with initial germination percent already known.
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