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Ultrasonic Carcass Assessment of Dorper and Dorper x Merino Lambs Using MLD and Body Measurements Full text
2016
Onur Yılmaz | Sezen Ocak | Sinan Ogun
Ultrasonic measurements of Musculus longissmus dorsi thoracis et lumborum (MLD) were taken of Dorper (n=99) and Dorper x Merino (n=65) lambs at 156 days of age and comparisons made to various body dimensions to ascertain whether sex, genotype, birth type and live weight had any effect on these parameters. Lambs were weaned two months after birth and placed ad libitum on basic dry land pasture during the draught spring and summer months of 2014 in the arid southeastern region of Turkey. Least square means of backfat thickness, skin+backfat thickness, muscle depth, body length, chest girth and live weight, at the mean age of 156 days, were 0.17 cm, 0.32 cm, 1.75 cm, 63.30 cm, 72.37 cm and 25.16 kg, respectively. Genotype was a statistically significant factor in terms of back fat thickness (BFT), skin and backfat thickness (S+BFT), muscle depth (MD), body length (BL) and live weight (LW). The study showed that Dorper and Dorper x Merino genotypes into Turkey show a clear advantage over the native breeds in terms of meat production and quality not to mention the high potential for lean carcass production. Ultrasonic measurements of Longissimus thoracis et lumborum (MLD) will provide an important contribution to selection index in breeding programs for Dorper and Dorper crossbred genotypes. However estimation of genetic parameters are required in containing a pedigree database in order to establish an effective selection index.
Show more [+] Less [-]Effects of Some Beneficial Bacteria in Casing Soil on Growth and Yield of Cultivated Mushroom Agaricus bisporus Full text
2016
Mehmet Çetin | Hatice Özaktan | Kaya Boztok
This research was carried out to determine the interaction between some bacteria naturally existing in casing soil and Agaricus bisporus (Sylvan Hauser A15) hypha in laboratory (in vitro) and cultivation (in vivo) conditions, and to confirm its effects on mushroom yield. Totally 32 bacteria (3 Gram (+) and 29 Fluorescent Pseudomonads) was isolated from casing soil and healthy sporophores. As a result of in vitro experiment carried out to determine the effects of bacteria on mycelium growth of A. bisporus, 24 bacterial isolates were found more effective at the rate of 2 to 115% than control treatment. To determine the effects of bacterium, chosen at the end of in vitro experiments, on mushroom yield in cultivation conditions, three experiments were established in March, May and July in 2008. At the end of experiments, bacterial isolates provided 8 – 40 % increase in total yield. Population density and change in population number related to time was observed during growing period, after the inoculation of bacterial isolates into casing soil. According to the results, Pseudomonas fluorescens (T 4/2 and Ş 8), P.putida (Ş 2/1 and Ş 10) and Bacillus mycoides (T 7/2) bacterial isolates were colonized successfully both in casing soil and sporophores.
Show more [+] Less [-]Factors Affecting Dairy Farmers’ Application of Agricultural Innovations: A Case Study from Muğla Province Full text
2016
Tayfun Çukur
The main objective of this study is to determine the factors affecting the application of innovations for dairy farmers in the Milas district, Muğla province, Turkey. Data from 71 dairy farmers and the Multinominal logit model are used for this study. The dependent variable of the model is divided into three categories; “I don’t apply any agricultural innovations”, “I apply agricultural innovations only after other farmers apply and express their positive opinion” and “I apply agricultural innovations”. In conclusion of the analysis, the comparisons are done with the farmers who “do not apply any agricultural innovations,” and the farmers who “apply agricultural innovations only after other farmers apply and express their positive opinion”; it is determined that a one unit increase in educational level raised the likelihood of applying innovations after receiving positive opinion. When the farmers that “apply agricultural innovation”, and that “apply agricultural innovations only after other farmers apply and express their positive opinion” are compared, it is found that a one unit increase in the number of milking animals had increased the likelihood of applying the innovations.
Show more [+] Less [-]Comparison of Fossil Fuel and Geothermal Energy Sources Used for Greenhouse Heating Full text
2016
Abdullah Nafi Baytorun | Derya Önder | Özkan Gügercin
The most extensive and effective environmentally controlled production takes place in greenhouses. New innovations and developments for greenhouse production go hand in hand with efforts for sustainability. The greenhouses must be heated to ensure high yields and high quality. However, high heating cost (20%-60%) adversely influences production. Costs Sustainability of greenhouse production can only be maintained with increase of energy efficiency. Use of renewable energy sources in place of fossil fuels is only means to increase energy efficiency. In this work, fossil fuels and geothermal energy used for heating of greenhouses are compared based on production cost and CO2 emissions to atmosphere. The results showed that greenhouse production in Aydın with geothermal energy price of 0.06 ₺/kWh will be very advantageous compared to greenhouse tomato production in Kütahya, which can only compete with that in Antalya. Carbon dioxide emission in plastic greenhouses with double layer PE plastic heat insulation in Kütahya was 199.4 kg/m2 when imported coal was used for heating. The emissions in similar type plastic greenhouses, again heated with coal, in Aydın and Antalya were 74.4 kg/m2 and 55.5 kg/m2, respectively. Greenhouses in geothermal energy rich areas will be very advantageous, based on cost of production and CO2 emission, compared to that in Antalya region if they are heated with geothermal energy.
Show more [+] Less [-]Sustainability Organic Agriculture and Livestock Production with Respect to European Union in Eastern Anatolia and East Black Sea Regions Full text
2016
Vecihi Aksakal | Sümer Haşimoğlu | Bahri Bayram | Yaşar Erdoğan | Hilal Ürüşan Altun | Mahir Murat Cengiz
Sustainability Organic Agriculture and Livestock Production with Respect to European Union in Eastern Anatolia and East Black Sea Regions Full text
2016
Vecihi Aksakal | Sümer Haşimoğlu | Bahri Bayram | Yaşar Erdoğan | Hilal Ürüşan Altun | Mahir Murat Cengiz
The majority of farm households in Turkey and especially the Eastern Anatolia are still based on low-input semi subsistence agriculture and livestock production. Despite a slow decline in recent years, agriculture and livestock production remains a major employer in Turkey and it is a significant contributor to the country’s gross domestic product, GDP. Whist Turkey is one of the EU candidate countries, is self sufficient in food production and Turkish agriculture is poorly structured inefficient, with farming in the Eastern Anatolia being mainly subsistence farming. Yet, these traditional rural structures combined with poor access to low level of education and low level of off-farm unemployment problem makes the situation more complicated and unsustainable. The best way to promote sustainability, better and higher production of Eastern Anatolian and rural Turkey is to invest in the local people, villages through improved, continuing and effective agricultural and livestock programs in particular. Investment in human capital especially in the rural areas leads to more employment opportunities through entrepreneurship and innovation in organic agriculture and livestock production. A holistic approach to developing and improving supply chains could unlock the potential for sophisticated, state-of-the-art organic agriculture and livestock producers and businesses in the region to become EU and global players. Eastern Anatolian livestock producers and the farmers have the ambitions to take part in future progress because the region is naturally organic not by design but default. It is for sure that present potential of the region has not been fully determined and utilized. EU has greatly benefited from previous enlargements economically, politically and socially. When European Union (EU) and Turkish Government relations considered and accession of Turkey to EU would be the logical consequence of the previous accessions. The screening on chapter 11 (Agriculture and rural development) is one of the important criteria and Turkey is working on to meet these benchmarks.
Show more [+] Less [-]Sustainability Organic Agriculture and Livestock Production with Respect to European Union in Eastern Anatolia and East Black Sea Regions Full text
2016
Aksakal, Vecihi | Hasimioğlu, Sümer | Bayram, Bahri | Dellal, Gürsel | Erdogan, Yasar | Altun, Hilal Ürüşen | Cengiz, M. Murat
The majority of farm households in Turkey and especially the Eastern Anatolia are still based on low-input semi subsistence agriculture and livestock production. Despite a slow decline in recent years, agriculture and livestock production remains a major employer in Turkey and it is a significant contributor to the country’s gross domestic product, GDP. Whist Turkey is one of the EU candidate countries, is self sufficient in food production and Turkish agriculture is poorly structured inefficient, with farming in the Eastern Anatolia being mainly subsistence farming. Yet, these traditional rural structures combined with poor access to low level of education and low level of off-farm unemployment problem makes the situation more complicated and unsustainable. The best way to promote sustainability, better and higher production of Eastern Anatolian and rural Turkey is to invest in the local people, villages through improved, continuing and effective agricultural and livestock programs in particular. Investment in human capital especially in the rural areas leads to more employment opportunities through entrepreneurship and innovation in organic agriculture and livestock production. A holistic approach to developing and improving supply chains could unlock the potential for sophisticated, state-of-the-art organic agriculture and livestock producers and businesses in the region to become EU and global players. Eastern Anatolian livestock producers and the farmers have the ambitions to take part in future progress because the region is naturally organic not by design but default. It is for sure that present potential of the region has not been fully determined and utilized. EU has greatly benefited from previous enlargements economically, politically and socially. When European Union (EU) and Turkish Government relations considered and accession of Turkey to EU would be the logical consequence of the previous accessions. The screening on chapter 11 (Agriculture and rural development) is one of the important criteria and Turkey is working on to meet these benchmarks.
Show more [+] Less [-]Genome wide analysis of stress responsive WRKY transcription factors in Arabidopsis thaliana Full text
2016
Shaiq Sultan | Muhammad Amjid Ali | Rana Muhammad Atif | Farrukh Azeem | Habibullah Nadeem | M. Hussnain Siddique | Ertuğrul Filiz | Khadim Hussain | Amjad Abbas
WRKY transcription factors are a class of DNA-binding proteins that bind with a specific sequence C/TTGACT/C known as W-Box found in promoters of genes which are regulated by these WRKYs. From previous studies, 43 different stress responsive WRKY transcription factors in Arabidopsis thaliana, identified and then categorized in three groups viz., abiotic, biotic and both of these stresses. A comprehensive genome wide analysis including chromosomal localization, gene structure analysis, multiple sequence alignment, phylogenetic analysis and promoter analysis of these WRKY genes was carried out in this study to determine the functional homology in Arabidopsis. This analysis led to the classification of these WRKY family members into 3 major groups and subgroups and showed evolutionary relationship among these groups on the base of their functional WRKY domain, chromosomal localization and intron/exon structure. The proposed groups of these stress responsive WRKY genes and annotation based on their position on chromosomes can also be explored to determine their functional homology in other plant species in relation to different stresses. The result of the present study provides indispensable genomic information for the stress responsive WRKY transcription factors in Arabidopsis and will pave the way to explain the precise role of various AtWRKYs in plant growth and development under stressed conditions.
Show more [+] Less [-]Consumers’ Perception About Genetically Modified Foods and Their Purchase Intention in the City Center of Hatay, Turkey Full text
2016
Ahmet Duran Çelik | Erdal Dağıstan
Consumers’ Perception About Genetically Modified Foods and Their Purchase Intention in the City Center of Hatay, Turkey Full text
2016
Ahmet Duran Çelik | Erdal Dağıstan
In this study consumers’ perception of, and purchase intention for genetically modified foods were examined in the city center of Hatay. The data of the 343 surveys were collected by using the face to face interview method. The data were analyzed by means of Likert Scale, and Spearman Correlation Analysis. According to the survey results, consumers’ risk perceptions about genetically modified foods are quite high. Consumers don’t willingly purchase genetically modified foods, and they intend to consume foods grown in traditional methods. High risk perceptions have a determining role on consumers’ views about genetically modified foods and their purchase intention for them. Another outcome from this study is that consumers’ awareness and knowledge levels about genetically modified foods are quite low, and that their perceptions and attitudes are mostly based on biases.
Show more [+] Less [-]Adaptación del arroz riego (Oryza sativa L.) en el Caribe colombiano Full text
2011
Aramendiz Tatis, Hermes(Universidad de Córdoba Facultad de Ciencias Agrícolas) | Espitia Camacho, Miguel(Universidad de Córdoba Facultad de Ciencias Agrícolas) | Cardona Ayala, Carlos(Universidad de Córdoba Facultad de Ciencias Agrícolas)
In Colombia, the rice crop under irrigation system ranks first in food security, economic value and employment offer among annual crops, becoming the most important. The objective of this study was to quantify increasing, stability and adaptability of irrigated rice in five departments of the Colombian Caribbean area. Data from the Ministry of Agriculture and Rural Development for the departments of Cordoba, Bolivar, Magdalena, Cesar and Guajira, during the year period 1987 to 2008 were used. The increasing in yield was determined using the linear regression analysis among years (independent variable) and grain yield (dependent variable) in each department. The yield stability was determined by the coefficient of variation across of three consecutive periods of five years and the last seven years. The yield adaptability was estimated using linear regression coefficient (bi). The results indicate that progress in grain yield in the Colombian Caribbean, ranged from 1.15% to 3.36% per year and 52.2 to 168.1 kg.ha-1, especially in the department of Bolívar (3.36% per year and 168.1 kg.ha-1). The yield stability was generally high (CV<15.56%) and increased with the years. In the five departments, the rice crop showed general adaptability (bi>4.57) for grain yield. | En Colombia, el arroz ocupa el primer lugar en seguridad alimentaria, valor económico y generación de empleo entre los cultivos anuales, siendo el sistema bajo riego más importante. El objetivo fue determinar el progreso, estabilidad y adaptabilidad del rendimiento de arroz bajo riego, en cinco departamentos del caribe colombiano. Se utilizaron datos del Ministerio de Agricultura y Desarrollo Rural, correspondiente a los Departamentos de Córdoba, Bolívar, Magdalena, Cesar y Guajira, durante el periodo 1987-2008. La estimación del progreso del rendimiento se realizó a través del análisis de regresión lineal entre los años (variable explicativa) y el rendimiento de grano (variable explicada). La estabilidad se determinó a través del coeficiente de variación para tres períodos consecutivos de cinco años y el último de siete. La adaptabilidad, se realizó con el coeficiente de regresión lineal (bi). Los resultados destacan que el progreso en el rendimiento de grano en el Caribe colombiano, osciló entre 1.15% y 3.36% por año y 52.2 y 168.1 kg.ha-1, especialmente en el Departamento de Bolívar (3.36% por año y 168.1 kg.ha-1). La estabilidad, resultó en general alta (CV<15.56%) y se incrementó con los años. Los cinco departamentos, presentaron adaptabilidad general (bi>4.57) para el rendimiento de grano.
Show more [+] Less [-]Study on Prevalence of Mycoflora in Wheat Seeds Full text
2016
Pratishtha Adhikari | Gopal Bahadur Khatri-Chhetri | Sundar Man Shrestha | Santosh Marahatta
Forty seed sample of wheat (Triticum aestivum) were collected from four locations viz. Chitwan, Kaski, Banke and Lalitpur and tested by blotter method at laboratory during 2013 for determining fungal pathogens associated with wheat seeds in Nepal. Eighteen species representing thirteen genera of fungi were recovered from the seed. Alternaria alternata and Bipolaris sorokiniana were predominant in all the varieties/genotypes from all the locations, where B. sorokiniana was strongly pathogenic in wheat crop. Percentage frequency and type of fungi detected varied with variety and locations. Bipolaris sorokiniana was highest (64.40%) in Banke than remaining three locations. Seeds of Chitwan had lowest percentage (5.50%) of seed infection as compared to other locations. Relative abundance of Alternaria alternata (55.10%) was highest as it was the most prevalent component of seed borne mycoflora, followed by Bipolaris sorokiniana (34.69%) and Cladosporium herbarum (7.19%). Differences in quantity of precipitation and relative humidity might be the possible reason for variation in frequency and type of fungi detected in wheat seeds of four locations.
Show more [+] Less [-]Effect of Growing Media on Yield and Fruitbody Properties of Hericium Isolates Full text
2016
Funda Atila | Yüksel Tüzel
Effect of Growing Media on Yield and Fruitbody Properties of Hericium Isolates Full text
2016
Funda Atila | Yüksel Tüzel
In the present study, it was aimed to determinate the effects of different substrates on mycelial growth, fructification, yield, sizes and colours of fruit bodies of Hericium isolates (HE-Ankara, HE-Denizli, HE, HE-Trabzon, HE-İzmit, HC, HE-Amerika). Experiments were conducted with 7 different substrates prepared with oak sawdust (MT), wheat bran (BK), cotton seed hulls (PK) and olive press cake (ZP) (80MT:20BK, 90MT:10PK, 80MT:20PK, 70MT:30PK, 90MT:10ZP, 80MT:20ZP, 70MT:30ZP). 1 kg (wet weight) of substrates were packed in polypropylene autoclaveable bags of 25x45 cm and sterilized in autoclave at 121oC during 90 minutes. Sterilized substrate was inoculated and then carried to growing room at 25±2ºC. After full colonization, the bags were exposed to 400 lux for a 12 hours photoperiod at 20±2ºC with a humidity of 80-90% in a cropping room. The best yield and BE were detected from oak sawdust medium supplemented with 20 and 30% cotton seed hulls on HE-Ankara, HE-Denizli, HE, HE-İzmit, HC, HE-Amerika isolates while the best yield and BE were detected from 70MT:30ZP on Trabzon isolate. Significant differences were found among substrates regarding yield, BE, average mushroom weigh, fruit body size and colour of Hericium isolates.
Show more [+] Less [-]Chamaerops humilis L. FRUIT PULP VALORIZATION: A DESIGN OF EXPERIMENTS APPROACH TO POWDER PRODUCTION AND PHYSICOCHEMICAL CHARACTERIZATION Full text
2024
Hamza Derraji | Badreddine El Mejhed | Fouzia Kzaiber | Wafa Terouzi
In response to demographic pressures and environmental imperatives, this study focuses on the valorization of Chamaerops humilis L. fruit in the Beni Mellal Khenifra region. The native palm, known as "Doum," holds nutritional and medicinal significance, prompting a detailed exploration. Extracts from its leaves and fruits have been historically used in traditional medicine, showcasing anti-oxidant properties and therapeutic effects on various ailments. This study seeks to optimize Chamaerops humilis L. fruit pulp powder production using the design of experiments (DOE) and physicochemical characterization. A full factorial design approach is applied to systematically optimize the process, focusing on key variables like Temperature and Time. Physicochemical analysis reveals essential details concerning pH (4.205), titratable acidity (1.47), moisture content (10.85%), and ash content (2.9325%). The study identifies optimal conditions, such as temperature = 65°C and time = 6 hours, for maximum yield and desirable properties. Additionally, water absorption (WAI) and solubility indices (WSI) are investigated, revealing the influence of drying and different agitation temperatures. The Pareto diagram underscores the significant impact of these factors, contributing to our understanding of the quality parameters of Chamaerops humilis L. powder. The results offer valuable insights for sustainable practices in utilizing this botanical resource.
Show more [+] Less [-]Detección del virus de la leucosis bovina en ganado criollo colombiano mediante PCR-anidado Full text
2011
Hernández-Herrera, Darwin Yovanny(Universidad Nacional de Colombia sede Palmira Facultad de Ciencias Agropecuarias) | Posso-Terranova, Andrés Mauricio(Universidad Nacional de Colombia sede Palmira Facultad de Ciencias Agropecuarias) | Benavides, Javier Antonio(Universidad Nacional de Colombia sede Palmira Facultad de Ciencias Agropecuarias) | Muñoz-Flórez, Jaime Eduardo(Universidad Nacional de Colombia sede Palmira Facultad de Ciencias Agropecuarias) | Giovambattista, Guillermo(, Universidad Nacional de la Plata Facultad de Ciencias Veterinarias) | Álvarez-Franco, Luz Ángela(Universidad Nacional de Colombia sede Palmira Facultad de Ciencias Agropecuarias)
Se evaluó la presencia del virus de la leucosis bovina (VLB) en 360 muestras de ADN de ocho razas bovinas criollas: Blanco Orejinegro (BON), Casanareño (CAS), Costeño con Cuernos (CCC), Chino Santandereano (ChS), Caqueteño (CQT), Hartón del Valle (HV), Romosinuano (RS) y San Martinero (SM), dos Razas Sintéticas Colombianas: Lucerna (LUC) y Velásquez (VEL) y dos razas foráneas: Brahmán (B) y Holstein (H). Para la detección del pro-virus se amplificó una región del gen env viral, mediante PCR anidada. La presencia del VLB fue mayor en la raza HV seguido por ChS (83.3% y 60% respectivamente), VEL y LUC tuvieron el mismo porcentaje (50%), en CAS, CCC y CQT la presencia del virus fue de 26.7%, 23.3% y 16.7% respectivamente; no se encontró el virus en BON, SM y RS. En las razas foráneas la presencia fue de 83.3% para H y 6.7% para B. Se encontró dependencia altamente significativa entre la presencia del VLB y la raza, el sexo y región de origen de la muestra. El promedio de presencia en las razas criollas fue menor que en las foráneas, menor en los machos que en las hembras y en la región norte que en el suroccidente y el centro del país. | Using 360 DNA samples from eight Creole bovine breeds Blanco Orejinegro (BON), Casanareño (CAS), Costeño con Cuernos (CCC), Chino Santandereano (ChS), Caqueteño (CQT), Hartón del Valle (HV), Romosinuano (RS) and San Martinero (SM), two synthetic Colombian breeds: Lucerna (LUC) and Velásquez (VEL) and two introduced breeds Brahmán (B) and Holstein (H); the presence of Bovine Leukemia Virus (BLV) was evaluated through the amplification of a viral gene region env (provirus detection - nested-PCR). The percentage of presence and independence test were calculated (X²). Presence of BLV was higher in HV breed, followed by ChS (83.3% and 60% respectively); VEL and LUC breeds showed the same percentage (50%). In CAS, CCC and CQT the presence of virus was 26.7%, 23.3% y 16.7% respectively. On the other hand, no virus presence was found in BON, SM and RS. For the introduced breeds the presence of virus was 83.3% for H and 6.7% for B. The average of presence for Creole bovine breeds was lower than introduced breeds. A high and significant dependence was found between the presence of BLV with breed, sex and sampling places. The presence was lower in males than in females and in the northern part than the southwestern and central areas of the country.
Show more [+] Less [-]Notes on Edibility of Tricholoma Species Full text
2016
Hakan Allı | İsmail Şen
Notes on Edibility of Tricholoma Species Full text
2016
Hakan Allı | İsmail Şen
The genus Tricholoma (Fr.) Staude is important in terms of hosting edible and poisonous species. Therefore, in this study, edible and poisonous Tricholoma species are evaluated, edible and valuable species are introduced. Also, poisoning syndromes are examined and discussed.
Show more [+] Less [-]Multiplicación clonal in vivo e in vitro de la especie forestal nativa Aniba perutilis Hemsl. Full text
2016
Delgado García, Lina Marcela(Universidad Nacional de Colombia Sede Medellín Facultad de Ciencias) | Hoyos Sánchez, Rodrigo Alberto(Universidad Nacional de Colombia Sede Medellín Facultad de Ciencias)
Se desarrolló una metodología para el establecimiento in vitro e in vivo de Aniba perutilis (comino crespo), árbol colombiano en peligro crítico de extinción. En la primera etapa, se sometieron semillas de este árbol a la acción de los desinfectantes NaOCl, HgCl2 y AgNO3 para su germinación aséptica. Para inducir la formación de brotes se empleó el medio WPM y se evaluó la acción de las fitohormonas BAP, KIN, AIA, ANA y AG3 en diferentes concentraciones. Para la etapa de enraizamiento se emplearon ANA, AIB, y los medios de cultivo MS y WPM, ambos con sales reducidas a la mitad y combinados con diferentes concentraciones de AIA y ANA. En la etapa de multiplicación in vivo se emplearon plántulas establecidas en vivero para evaluar tres tamaños de explantes, categorizados según la distancia desde la base de la planta hasta el ápice: distal (11-14 cm), medio (7 - 8 cm) y basal (3 - 4 cm), con el fin de obtener plantas madres donantes de yemas axilares y mini esquejes para enraizar. En este trabajo se consolida el uso de NaOCl por 15 min como tratamiento óptimo de desinfección, produciendo un porcentaje de supervivencia superior a 60%. El medio WPM suplementado con BAP 3 mg/lt y 1.5 mg/lt de AG3 generó la producción de un promedio de 0.6 brotes/explante con longitud media de 0.82 cm; no obstante, su baja capacidad de enraizamiento permite catalogar esta especie como recalcitrante al cultivo in vitro. En condiciones in vivo, cortes a la altura media de las plantas (7 - 8 cm) promovieron la formación de 1.33 brotes/explante y 75% de enraizamiento en los mini-esquejes, constituyendo una herramienta clave en la propagación de la especie | A methodology for the in vitro and in vivo establishment of Aniba perutilis, a native colombian tree which is currently critically endangered, was performed. In the first stage seeds were treated using NaOCl, HgCl2, and AgNO3 to achieve aseptic germination. In order to induce shoot formation, WPM medium was used and phytohormones BAP, KIN, IAA, NAA and GA3 were evaluated at different concentrations. In the rooting stage NAA and IBA were used in different concentrations and culture media, as well as half-strength MS and WPM mediums combined with different concentrations of IAA and NAA. For in vivo multiplication stage, juvenile plants previously established in nursery were used to evaluate three types of cuts defined by the distance between the base of the plant and where the cut was done: distal (11-14 cm), middle (7-8 cm) and basal (3-4 cm), in order to obtain donor plants able to donate axillary buds and mini-cuttings for future rooting. The use of NaOCl for 15 minutes consolidates as optimal disinfection treatment, yielding a survival rate greater than 60%. The WPM medium supplemented with BAP (3 mg/L) and GA3 (1.5 mg/L) allowed the production of 0.6 shoots/explant with an average length of 0.82 cm. However, the low rooting capacity of the explants suggests that this species could be labeled as recalcitrant to in vitro culture. In vivo conditions, cuts made at the middle height of the plants promoted the formation of 1.33 shoots/explant and 75% rooting in mini-cuttings, becoming these results in a key tool for the propagation of the species
Show more [+] Less [-]Clonal multiplication in vivo and in vitro of the native forest species Aniba perutilis Hemsl. | Multiplicación clonal in vivo e in vitro de la especie forestal nativa Aniba perutilis Hemsl. Full text
2016
Delgado-García, Lina Marcela | Hoyos-Sánchez, Rodrigo Alberto
A methodology for the in vitro and in vivo establishment of Aniba perutilis, a native colombian tree which is currently critically endangered, was performed. In the first stage seeds were treated using NaOCl, HgCl2, and AgNO3 to achieve aseptic germination. In order to induce shoot formation, WPM medium was used and phytohormones BAP, KIN, IAA, NAA and GA3 were evaluated at different concentrations. In the rooting stage NAA and IBA were used in different concentrations and culture media, as well as half-strength MS and WPM mediums combined with different concentrations of IAA and NAA. For in vivo multiplication stage, juvenile plants previously established in nursery were used to evaluate three types of cuts defined by the distance between the base of the plant and where the cut was done: distal (11-14 cm), middle (7-8 cm) and basal (3-4 cm), in order to obtain ‘donor plants’ able to donate axillary buds and mini-cuttings for future rooting. The use of NaOCl for 15 minutes consolidates as optimal disinfection treatment, yielding a survival rate greater than 60%. The WPM medium supplemented with BAP (3 mg/L) and GA3 (1.5 mg/L) allowed the production of 0.6 shoots/explant with an average length of 0.82 cm. However, the low rooting capacity of the explants suggests that this species could be labeled as recalcitrant to in vitro culture. In vivo conditions, cuts made at the middle height of the plants promoted the formation of 1.33 shoots/explant and 75% rooting in mini-cuttings, becoming these results in a key tool for the propagation of the species. | Se desarrolló una metodología para el establecimiento in vitro e in vivo de Aniba perutilis Hemsl. (comino crespo), árbol colombiano en peligro crítico de extinción. En la primera etapa, se sometieron semillas de este árbol a la acción de los desinfectantes NaOCl, HgCl2 y AgNO3 para su germinación aséptica. Para inducir la formación de brotes se empleó el medio WPM y se evaluó la acción de las fitohormonas BAP, KIN, AIA, ANA y AG3 en diferentes concentraciones. Para la etapa de enraizamiento se emplearon ANA, AIB, y los medios de cultivo MS y WPM, ambos con sales reducidas a la mitad y combinados con diferentes concentraciones de AIA y ANA. En la etapa de multiplicación in vivo se emplearon plántulas establecidas en vivero para evaluar tres tamaños de explantes, categorizados según la distancia desde la base de la planta hasta el ápice: distal (11-14 cm), medio (7 - 8 cm) y basal (3 - 4 cm), con el fin de obtener plantas madres donantes de yemas axilares y mini esquejes para enraizar. En este trabajo se consolida el uso de NaOCl por 15 min como tratamiento óptimo de desinfección, produciendo un porcentaje de supervivencia superior a 60%. El medio WPM suplementado con BAP 3 mg/lt y 1.5 mg/lt de AG3 generó la producción de un promedio de 0.6 brotes/explante con longitud media de 0.82 cm; no obstante, su baja capacidad de enraizamiento permite catalogar esta especie como recalcitrante al cultivo in vitro. En condiciones in vivo, cortes a la altura media de las plantas (7 - 8 cm) promovieron la formación de 1.33 brotes/explante y 75% de enraizamiento en los mini-esquejes, constituyendo una herramienta clave en la propagación de la especie
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