Molecular approaches used to identify bacterial species use 16S rRNA and MLST to determine the genetic linkage of bacteria; MLST characterizes clonal linkages by examining differences in various gene loci. MLVA determines the genetic relationships of bacterial strains and biovar-level differences and assesses the copy number of repeated DNA sequences. Sequencing provides genetic data by identifying DNA sequences; Sanger sequencing is the basis for next-generation approaches. CRISPR modifies the genetic code and can correct mutations or control genes using Cas9. These methods are important for identifying bacterial species and annotating genomic information. The methods used for this purpose are brought together in this study. The explanation and detailed description of the methods examined will contribute to their use in the field of microbiology.

In the arid conditions of the Steppe of Ukraine, the species composition of weeds characteristic of this soil-climatic zone develops. The correctly chosen method of basic soil tillage determines the effectiveness of weed suppression while simultaneously improving the growing conditions for sunflower varieties and hybrids. The purpose of the study was to identify the species composition of weeds in the steppe zone and establish the effectiveness the methods of basic tillage of soil in the technology of growing sunflower hybrids aimed at suppressing weeds and reducing the quantity of weeds in agrocenoses. Research has established that weeds of sunflower agrophytocenoses are represented by 78 species that belong to 2 classes, 18 orders, 27 families, 62 genera. In the agrophytocenosis of sunflower, the largest number of 93.5-96.3% is represented by dicotyledonous weeds (Magnoliopsida). The species composition is dominated by weeds of the Compositae family (Asteraceae) of the dicotyledonous class (Magnoliopsida), which leads to a significant decrease in the yield level. According to the species composition, 3 groups of weeds of the Asteraceae family (Asteraceae) were identified, of which 1 group of weeds is the most harmful and leads to the formation of a minimum yield of sunflower hybrids Yason and Daryi of 1.77-1.79 t/ha. The use of non-moldboard tillage leads to a percentage increase in the share of air dry mass of weeds of the Asteraceae family by 11.1-13.1%, while reducing the yield of sunflower hybrids by 0.13-0.21 t/ha. Thus, with the established species composition of weeds and the quantity of weeds, the use of plowing in sunflower cultivation technology makes it possible to form a maximum yield of 2.07-2.24 t/ha, providing more effective weed suppression in sunflower agrophytocenoses with a minimum percentage of air-dry mass of weeds of the Asteraceae family to the total number of weeds of 21.1-24.1%.

Agriculture is a cornerstone of the economy, providing livelihoods for a significant portion of population. However, climate change significantly affects people, their lifestyles, and the ecosystems posing a critical challenge to the global community, particularly the underprivileged in developing nations. Recognizing the indispensable role of agriculture and the challenges posed by a changing climate, this paper emphasizes the paramount need for proactive adaptation strategies. Central to these strategies is the pivotal concept of Climate-Smart Agriculture (CSA), a multifaceted approach that encompasses a range of practices, including agroforestry, conservation agriculture, and the adoption of climate-resilient crop varieties. Delving deeper, the paper navigates through the farmer's perceptions, unraveling their understanding of climate change, and the complex barriers like social barriers, institutional limitations, financial barriers, and limited awareness that impede effective adaptation, and illuminates the instrumental roles that governmental bodies and institutions, and extension agents play in shaping and fostering climate-resilient practices. Collaboration between local communities, governments, and non-governmental organizations is essential to ensure the successful implementation of sustainable adaptation strategies. Embracing sustainable and forward-thinking approaches, particularly CSA, including agroforestry, conservation agriculture, water management techniques, climate-resilient crop varieties, ICT, and climate-smart pest management, the agricultural sector gains the potential to bolster its resilience against climate-induced disruptions, ensuring consistent agricultural output that contributes significantly to broader food security initiatives.

Climate change has become one of the most important threats to worldwide agricultural production systems. This paper evaluates how Bangladesh, a prominent developing country in the low-lying Ganges-Brahmaputra-Meghna delta, is susceptible to climate change and assesses present agricultural practices that target sustaining production under these threats. The study synthesizes the ongoing research findings of climatic change tendency, involving the rising of temperature, alteration of precipitation pattern, along with the onward frequency of extreme weather incidence and their complication to crop production. It discusses the key susceptibilities of Bangladesh’s crop sector, such as a shortage of irrigation water, the impact of rising temperatures, increasing sea levels and the loss of biodiversity. Moreover, the study explores the resilience strategies and measures adopted by farmers, policymakers and researchers to alleviate the hostile effect of climate change on crop production. With all these considerations, the paper aimed to analyse the current climate change trend, adverse effects to agricultural sectors and existing resilience practices in Bangladesh as well as future strategies against climate change.

According to the purpose of use, the buildings are named with names such as residences, commercial buildings, health buildings, social structures, educational buildings, sports buildings, worship buildings, etc., and classifications are made for agricultural buildings as rural structures, animal-plant production structures and protection-storage structures. It has become important to determine the type and thickness of the insulation material that will provide the most economic benefit to reduce the energy costs consumed by reducing thermal losses/gains in all building types and to reduce the damage caused by waste gases to people, the environment and especially agricultural production. In order to reduce thermal losses and gains in building exterior walls and to reduce heating-cooling costs, it has become important to determine the type and thickness of insulation material that will provide the most economic benefit. In this study, optimum insulation thicknesses, annual savings, payback period and reduction in CO2 emissions were calculated for heating and heating-cooling application in Tokat. DD method and LCA method were used for thermal and economic analysis. XPS and RW were chosen as insulation materials according to two different wall types, natural gas for heating and electricity for cooling as energy sources. The payback period and CO2 emission were calculated with a lower value in the use of XPS insulation material than in RW insulation material. For this reason, it has been determined that it is more appropriate to prefer XPS insulation material in terms of economy and environment only in heating and heating-cooling application.

Anthropogenic activities with increasing population lead the pollution of ecosystems. Over one-third of the world's water resources are utilized for agricultural, domestic, and industrial activities, resulting in contamination by synthetic, and geogenic compounds such as dyes, fertilizers, pesticides, and heavy metals. Among these pollutants, dyes are particularly noteworthy due to their extensive use across various sectors, making them one of the leading contributors to water pollution. For this reason, dyes are one of the most important pollutants that cause water pollution. Therefore, the adsorption of Bromophenol blue (BPB) was studied in this study. Firstly, PGMA gels were produced by polymerizing of glycidyl methacrylate (GMA) monomer. Secondly, the PGMA gels were modified to prepare the new adsorbents for the adsorption of BPB dye. Thirdly, the adsorption of BPB dye was carried out. The batch adsorption method was used. The optimum adsorbent amount, initial BPB concentration, pH, and temperature parameters for PGMA gels were determined. The adsorption mechanism between modified PGMA gels, and BPB dye was elucidated by Langmuir, Freundlich, Dubinin-Radushkevich, and Temkin isotherm models. As a result, it was seen that modified PGMA gels showed good performance in the adsorption of BPB.

Bu çalışmada Trabzon hurması (Diospyros kaki L.) püresinin akış davranışını en iyi tanımlayan reolojik modelin belirlenmesi amacıyla pürenin reolojik özellikleri çeşitli pH’larda (4,0, 5,5 ve 7,0), konsantrasyonlarda (%15, %17,5 ve %20), sıcaklıklarda (25, 50 ve 75°C) ve kayma hızında (8,4–28 s−1) araştırılmıştır. Deneysel veriler Power Law, Herschel-Bulkley, Casson ve Mizhari-Berk modellerine uygulanmıştır. Reolojik modellerin akış davranışını açıklamadaki uygunluğunu doğrulamak için belirleme katsayısı (R2), hata kareleri ortalamasının kare kökü (RMSE) ve indirgenmiş ki- kare (ꭓ2) olmak üzere üç istatistiksel ölçüt kullanılmıştır. Herschel-Bulkley ve Mizhari-Berk, hurma püresinin tüm işlem koşullarında deneysel verilere en iyi uyum sağlayan modeller olduğu görülmüştür. Ancak Mizhari-Berk modeli R2 ≥ 0.983, RMSE ≤ 0.0683 ve ꭓ2 ≤ 0.0160 istatistiksel parametre değerleriyle hurma püresinin akış davranışını en iyi tanımlayan model olmuştur. Trabzon hurması püresi psödoplastik (kayma incelmesi) ve Newtonyen olmayan bir davranış (n<1) sergilemiştir.

Mevcut çalışma, Kahramanmaraş ilinin Onikişubat ilçesinde yetiştiricilik yapan 25 Kıl keçisi yetiştiricisinin gönüllü katılımıyla 2018 yılında 4867 baş teke altı keçi ve 230 baş damızlık teke ile başlatılmış ve çalışma beş yıl boyunca sürdürülmüştür. Bu çalışma ile halk elinde yetiştirilen Kıl keçilerinin döl verimi ve büyüme özellikleri bakımından ıslahı amaçlanmıştır. Bu amaçla, keçilerde belirlenen döl verim kriterleri ile oğlakların doğum ağırlıkları, sütten kesim canlı ağırlıkları (90 gün), günlük canlı ağırlık kazançları ve yaşama gücü gibi büyüme özellikleri yıl bazında kaydedilmiştir. Her yıl sürüye eklenecek damızlık materyal seçimlerinde Kıl keçisi ırkına ait morfolojik özellikler ve oğlakların doğum ve sütten kesim ağırlıkları dikkate alınarak en iyi büyüme ve damızlık özelliği gösteren erkek oğlakların yaklaşık %10’u dişi oğlakların ise %50’si damızlık olarak seçilmiştir. Çalışmanın sonunda, yıllara göre doğum oranının %54,21 ile %76,93, ikizlik oranının %21,64 ile %27,19, oğlak veriminin %65,94 ile %94,66 ve doğum başına düşen oğlak sayısının 1,22 ile 1,27 arasında olduğu tespit edilmiştir. Kıl keçisi oğlaklarının yıllara göre doğum ağırlıkları 3,11 kg ile 3,19 kg, sütten kesim ağırlıkları 17,09 kg ile 18,63 kg, sütten kesime yaşına kadar günlük canlı ağırlık kazançları 154,63 ile 171,55 g ve yaşama gücünün %92,27 ile %95,34 arasında olduğu saptanmıştır. Bu sonuçlara göre oğlakların büyüme performansı (P<0,001) ve yaşama gücü değerlerinde çalışmanın başladığı yıla göre diğer yıllarda artışlar sağlanmıştır. Ayrıca, büyüme performansı ve yaşama gücü dikkate alındığında, cinsiyete ve doğum tipine bağlı olarak erkek ve tek doğan oğlakların sırasıyla dişi ve ikiz doğan oğlaklara kıyasla daha yüksek performans ve yaşama gücü değerleri sağladığı belirlenmiştir.

At the Jimma town’s Boye nursery site in southwest Ethiopia, three selected indigenous agroforestry tree species were the subject of a study to determine how their foliage litter materials broke down over time. Using Pearson’s correlation coefficient, the study used a single exponential model to calculate the rate at which decomposing litter materials decayed and the litter chemical quality indices that were investigated. C. macrostachus had a rate constant of 0.0400 day-1, which was substantially faster than that of F. vata and E. abyssinica. It took 17.3 and 29.7 days, respectively, for F. vata and E. abyssinica to lose 50% of the initial dry matter. While nitrogen and phosphorus were found to be facilitators, the parameters of the chemical quality of the litter were found to be impeding biochemical parameters. The species with the fastest rate of nitrogen return in a short amount of time was found to be E. abyssinica, followed by C. macrostachus and F. vasta. C. macrostachus decomposes quickly, which may limit its ability to improve soil fertility over an extended period of time. It is important to take into account F. vasta and primarily E. abyssinica for the long-term accumulation of soil organic carbon.

Ruminant hayvanların beslenmesinde kullanılan rasyonlar belirli oranlarda kaba ve kesif yem içermelidir. Besin madde içeriği bakımından zengin olan kesif yemler genelde rasyon maliyetini artırmaktadır. Hayvancılık işletmelerinde bu yüksek maliyetten kurtulmak kaliteli kaba yem üretimine bağlıdır. Ülkemizde faaliyet gösteren hayvancılık işletmeleri yem (besleme) maliyetlerinin azaltılması ve ürün kalitesinin yükseltilmesi amacı ile farklı kaba yem arayışı içindedirler. Bu çalışmanın konusunu teşkil eden soya bitkisi, ruminant rasyonlarında hasıl veya silaj formunda kullanılma potansiyeli olan bir kaba yem türüdür. Bir baklagil yem bitkisi olan soya bitkisi protein içeriğinin yüksek olması (yaklaşık %20) ile kaba yem grubu yemler içinde önemli bir yere sahiptir. Soya bitkisi bu özelliği ile ruminant rasyonlarında temel protein kaynağı olarak kullanılan bazı yem kaynaklarının bir kısmı yerine ikame edilebilmektedir. Nitekim bazı çalışmalarda soya silajının en kaliteli protein kaynağı olarak kabul edilen soya küspesinin yerine bile kısmen de olsa kullanılabileceği ortaya konulmuştur. Bu derlemede soya bitkisinin ruminant rasyonlarında kaba yem kaynağı (hasıl ve silaj) olarak kullanılma potansiyeli hakkında bilgi verilmiştir.