The belief that ancient wheat is more beneficial than common wheat has been growing trend in recent years among the consumers. The present study aims to compare bioactive peptide, allergen peptide and toxic peptide generation after gastrointestinal digestion of modern wheat (Triticum aestivum) and ancient wheat, einkorn (Triticum monococcum var. monococcum), using in silico tools. The primary sequences of both kinds of wheat were obtained from BIOPEP-UWM and UniProtKB/Swiss-Prot database. In silico digestion was applied using BIOPEP-UWM online tool. For the simulation of gastrointestinal digestion pepsin (pH 1.3) (EC 3.4.23.1), trypsin (EC 3.4.21.4), and chymotrypsin (EC 3.4.21.1) were selected and analyzed. Homology analysis was performed for each protein sequences using EMBOSS Needle program. Toxic and allergen peptides were predicted using ToxinPred online tool and Allergen FP v.1.0. The results showed that einkorn and common wheat proteins exhibited similar properties including high similarity rate (58.72-87.40%) indicating the percentage of matches between the two sequences and the identical bioactivities for peptides generated after digestion. Most of the bioactive peptides were dipeptides and the majority of them displayed more than one bioactivities including ACE inhibitory, DPP IV inhibitory or antioxidant activity, etc. Allergen peptides generated after in silico digestion were found to be similar for both kinds of wheat. In silico gastric digestion of einkorn and wheat caused toxic peptides production, but they were disappeared after in silico intestinal digestion. In conclusion, although there is a perception related to the Einkorn that is healthier than common wheat, in silico digestion of common wheat and einkorn did not support this perception.

Se evaluaron familias de hongos formadores de micorriza arbuscular (HMA) asociados a dos variedades de aguacate Persea americana Mill (hass y lorena), en 12 muestras de suelo rizosférico y raíces en Caldas, Colombia. Las esporas se obtuvieron por tamizado húmedo y centrifugación en gradiente de sacarosa, y montadas en los reactivos Polivinilactoglicerol y Melzer para determinar su morfología, y las raíces tratadas con hidróxido de potasio, ácido acético, azul de tripano y lactoglicerol, para establecer su colonización. El ADN fue extraído por DNeasy Plant Mini Kit (Qiagen™) y amplificado por PCR utilizando iniciadores sobre los genes 18S y 28S del ADNr nuclear, visualizado y purificado en geles de agarosa, y secuenciado por Macrogen Advancing Through Genomics-Corea del Sur. Los resultados muestran presencia de 12 morfotipos pertenecientes a las familias Acaulosporaceae, Gigasporaceae, Diversisporaceae y Glomeraceae, y una colonización micorrízica superior al 90% en ambas variedades de aguacate. Se logró aislar ADN de nueve morfotipos y amplificar siete. El alineamiento de las secuencias de ADN se realizó en el programa ClustalW incluido en el programa Mega 6, junto con secuencias depositadas en el GenBank, utilizando el parámetro de distancia Kimura 2 (K2P), permitieron construir un árbol de similitud por el método Neighbor-Joining (NJ) agrupando las muestras determinadas por morfología en las familias correspondientes. Estos resultados permitieron estandarizar protocolos para familias de este grupo, donde su taxonomía es dificultosa y controversial, registrando familias citadas en la literatura como de elevada importancia para la protección e incorporación de nutrientes en la planta. | Families of arbuscular mycorrhizal fungi (AMF) associated to two varieties of avocado Persea americana Mill (hass and lorena), in 12 samples of rhizosphere soil and roots in Caldas, Colombia were evaluated. Spores were obtained by wet sieving and sucrose gradient centrifugation, and mounted on polyvinyl alcohol using glycerol and Melzer's reagents, to determine their morphology, and roots treated con potassium hydroxide, acetic acid, Trypan Blue and lactoglycerol, to establish colonization. The DNA was extracted by DNeasy Plant Mini Kit (Qiagen™) and amplified by PCR using primers for genes 18S and 28S nuclear rDNA, visualized and purified on agarose gels and sequenced by Macrogen Advancing throughout Genomics-South Korea. The results show presence of 12 morphotypes belonging to the families Acaulosporaceae, Gigasporaceae, Diversisporaceae and Glomeraceae, and mycorrhizal colonization higher 90% in both varieties of avocado. It was possible to isolate DNA of nine morphotypes and amplify seven. Alignment of DNA sequences carried out in the ClustaW software included in the program Mega 6, together with sequences deposited in GenBank, using the distance parameter Kimura 2 (K2P), they allowed to build a tree of similarity for the method Neighbor-Joining (NJ) clustering the samples determined by morphology in the corresponding taxonomical families. These results allowed to standardize protocols for families in this group, where his taxonomy is difficult and controversial, registering families cited in the literature as highly important for the protection and incorporation of nutrients into the plant.

The depletion of minerals in agricultural soils through subsistence farming has been a major food security challenge in many parts of the world. To curtail this problem, farmers use inorganic fertilizer to boost soil fertility even though it poses a lot of environmental challenges. In this research, an alternative route to soil nutrient amendment was explored via the use of compost and nano-gel water accumulator for blending different soil samples derived from rock side soil and spent farm soils blended in different ratios of 1:0, 1:1, 1:2, and 2:1using a suitable potting media for the greenhouse production of A. spinosus L. Physicochemical values, mineral, and heavy metals concentration were evaluated on the soil and compost samples while mineral, proximate, anti-nutrients and vitamins compositions were analyzed on A. spinosus L. grown on the soils. Data obtained were analyzed using analysis of variance (ANOVA) at a 95% confidence limit using SPSS 20.0 software. The properties of the prepared compost (PC) and commercial compost (CC) varied significantly (P

Sapodilla (Manilkara zapota (L.) P. Royen) is a climacteric fruit, very perishable, with high metabolic activity, rapid ripening and reduced storage time at room temperature, thus making difficult its commercialization. However, this research aimed to evaluate the effect of potassium permanganate (KMnO4) on the quality and post-harvest conservation of sapodilla fruit under modified atmosphere at room temperature. The sapodilla fruits were harvested when they reached physiological maturity and then accommodated in Styrofoam trays with PVC film coating. Within each package, KMnO4 were placed at different concentrations (0, 0.250, 0.375, and 0.500 g of KMnO4 per kg of fruit, respectively), impregnated in vermiculite. In addition, were kept in storage at room temperature (25 ± 1 °C and 54 ± 5% RU) for fifteen days, with fruit quality analysis every five days. The potassium permanganate doses influenced the sapodilla fruit quality, wherein the concentration of 0.375 g of KMnO4 per kg of fruit was the most efficient, retarding the loss of firmness and vitamin C degradation of sapodilla fruits throughout five days storage under modified atmosphere at room temperature. The sapodilla fruit postharvest life stored under modified atmosphere with potassium permanganate 25 ± 1 °C and 54 ± 5% RU, is ten days. | Sapodilla (Manilkara zapota (L.) P. Royen) is a climacteric fruit, very perishable, with high metabolic activity, rapid ripening and reduced storage time at room temperature, thus making difficult its commercialization. However, this research aimed to evaluate the effect of potassium permanganate (KMnO4) on the quality and post-harvest conservation of sapodilla fruit under modified atmosphere at room temperature. The sapodilla fruits were harvested when they reached physiological maturity and then accommodated in Styrofoam trays with PVC film coating. Within each package, KMnO4 were placed at different concentrations (0, 0.250, 0.375, and 0.500 g of KMnO4 per kg of fruit, respectively), impregnated in vermiculite. In addition, were kept in storage at room temperature (25 ± 1 °C and 54 ± 5% RU) for fifteen days, with fruit quality analysis every five days. The potassium permanganate doses influenced the sapodilla fruit quality, wherein the concentration of 0.375 g of KMnO4 per kg of fruit was the most efficient, retarding the loss of firmness and vitamin C degradation of sapodilla fruits throughout five days storage under modified atmosphere at room temperature. The sapodilla fruit postharvest life stored under modified atmosphere with potassium permanganate 25 ± 1 °C and 54 ± 5% RU, is ten days.

Bu çalışmada besinsel lif ve β-glukan yönünden zengin fonksiyonel bir gıda olan yulaf ezmesi ile fenolik maddelerce zengin, yüksek antioksidan içeren yaban mersini kurusu çikolatada katkı olarak kullanılmıştır. Yaban mersini kurusu ve yulaf ezmesi 1:1 oranında karıştırılarak elde edilen karışım sütlü kuvertür çikolataya %10, 20, 30, 40, 50 oranlarında ilave edilmiş ve kalorisi düşük ve sağlıklı bir ürün elde edilmesi amaçlanmıştır. Hazırlanan katkılı çikolata örneklerinin nem, kül, karbonhidrat, yağ, protein, selüloz, şeker ve enerji değerleri saptanmış ve örneklerin genel görünüş ve genel kabul edilebilirlik özellikleri duyusal olarak incelenmiştir. Çalışma sonucunda kuvertür çikolataya eklenen yulaf ezmesi ve yaban mersini kurusu karışımı örneklerinin nem değeri (%0,35-4,90), kül içeriği (%2,24-1,43), karbonhidrat değeri (%55,82-66,57), yağ miktarı (%33,20-20,05), protein miktarı (%8,49-7,09), selüloz miktarı (1,03-1,90), şeker miktarı (%39,30-46,87) ve enerji değerleri (%467,57-550,86) olarak belirlenmiştir. Duyusal analiz sonuçlarına göre ise yulaf ezmesi ve yaban mersini kurusu karışımının çikolatadaki miktarı arttıkça tercih edilme oranının azaldığı görülmüştür.

The effect of the two tractor wheel arrangements-double field wheel/single furrow wheel (double Re/single RS) and single field wheel/single furrow wheel (single RC/single RS,) on the effective field capacity in ploughing, was studied in order to prove that the arrangement double RC/single RS provides less costs per hectarea and greater effective field capacity. The field trials were carried out in eigth plots of 3750 m2 each one, employing two treatments (tractor wheel arrangements) and four replications. The tractor wheel arrangement double RC/single RS showed the greatest effective field capacity and the lowest costs per hectarea in comparison with the arrangement single RC/single RS. | Se estudió el efecto de los arreglos rueda de campo doble/rueda de surco sencilla (RC doble/ RS sencilla) y rueda de campo sencilla/rueda de surco sencilla (RC sencilla/RS sencilla) sobre la capacidad de campo efectiva (CCE) en labor de arada, bajo la hipótesis general de que  con doble rueda motriz de campo es de esperar una mayor capacidad de campo efectiva, debido a la reducción en el patinaje del conjunto tractor-arado. Se empleó un arado de tiro de discos con un ancho de corte de 90 cm, arando a una profundidad promedia de 26 cm. El arado se operó con un tractor de 45 k W. El ensayo se realizó en un lote de 5 ha de 200x250 m, el cual se aró por el sistema de melgas en el sentido más corto, para poder trazar ocho melgas de 25 m de ancho cada una. Las melgas hicieron las veces de parcelas experimentales, cada una con un área de 3750 m2; el ensayo se planeó con dos tratamientos (arreglos de ruedas) y cuatro replicaciones (melgas). En cada melga se midió el tiempo de campo, descontando aquellas pérdidas que no se debieron a los tratamientos aplicados (pérdidas debidas a fallas en el equipo que normalmente se presentan en este tipo de labores), con el fin de conservar la igualdad de condiciones en todas las parcelas. Con el arreglo RC doble/RS sencilla se obtuvo mayor capacidad de campo efectiva en comparación con el arreglo RC sencilla/RS sencilla; al hacer análisis de varianza la diferencia fue significativa (P≤ 0.05). También se registró una diferencia en costos de $1.266/ha menos a favor del arreglo RC doble/RS sencilla.

Bovine leukemia virus (BLV) is known as the causative agent of enzootic bovine leukosis (EBL), which is a worldwide distributed disease and has also been detected in marketed beef and dairy products. BLV causes significant economic losses due to the loss of milk and yield or the slaughter of animals without adequate development. It has been reported in epidemiological studies that this infection is common in Türkiye, especially in the western provinces. There is no data on the possible presence or prevalence of BLV infection and its seroepidemiology in Muş province. The aim of this study is to determine the possible presence and prevalence of the infection, its role in yield losses, and to obtain epidemiological data on cattle farming in the Muş district. For this purpose, 300 blood serum samples were collected from cattle aged six months and older in the province of Muş and its different districts. The blood serum samples taken were tested for the presence of BLV-specific antibodies by agar gel immunodiffusion (AGID) and competitive enzyme-linked immunosorbent assay (C-ELISA) methods. As a result of the study, all of the controlled districts were evaluated as negative in the AGID and C-ELISA tests for the presence of BLV-specific antibodies. In conclusion, for the first time, it was demonstrated that cattle farming in the Muş province were BLV-free during the sampling period. Although BLV seropositivity was not detected in the tested animals, it is emphasized that the control of infection and eradication program should not be ignored.

Los cortes se realizaron a los 28, 35 y 42 días en la hacienda Lucerna, norte del Valle del Cauca, Colombia (960 msnm, 24°C, 1100 mm anuales, 75% 80% de humedad relativa, 3.6 mm de evaporación promedia mínima diaria. El diseño experimental fue de parcelas divididas con cuatro repeticiones, con dos épocas secas y dos de lluvias. La altura en estrella asociada fue significativamente superior (P

The objective of this study is to diagnose the potato sector in the El Oued region by highlighting the technical and environmental situation of this sector. For this purpose, a field survey was carried out at the level of 75 farms in the El Oued region. The Oued Souf region’s Farm Sustainability Indicator (IDEA) is important. The assessment of agro-ecological, socio-territorial and economic indicators indicates that the agro-ecological indicator is the most represented by (the indicator of fertilizer and agricultural practices) by 98 out of 100. The results of this study show that this crop presents a good adaptation to the conditions of the region. This sector is undergoing significant development and is of great importance because this agricultural activity represents an economic potential at the local, regional and national levels. However, this sector remains fragile in relation to various constraints of a technical nature (unreasonable irrigation and fertilization and high seed prices), economic (price fluctuations and disorganized markets) and environmental (diseases, weeds) which directly influence the functioning of farms and therefore their profitability. This is why it is necessary to face the problems encountered and fight against the factors that can hinder the sustainability of this sector and give a competitive value.

The prices of raw materials necessary for production play an important role in the production decisions of farmers, and thus in shaping the level of supply of agricultural raw materials. The constant increase in the prices of raw materials for production increases its costs. Therefore, it should be noted that the rising prices of fuel and fertilizers affect the decisions and profitability of plant production. In connection with the above, this study attempts to indicate the impact of changes in fertilizer and fuel prices on the profitability of production of selected agricultural crops in Poland. The paper uses secondary data on the price level of fertilizers, fuels and selected agricultural products, as well as the structure of their production costs. Based on the sensitivity analysis, the potential impact of the increase in fuel and fertilizer prices on the profitability of the production of selected species was indicated. The results showed that the increase in the prices of the above-mentioned raw materials can significantly affect the economic efficiency of the production activity.

In this study, the reproductive and growth performance of Akkaraman sheep between 2017-2021 were evaluated within the scope of the National Sheep and Goat Breeding Project in the Hand of the Public, which is being carried out in the province of Nigde. In the research, data obtained from 30923 animals in 22 farms in 7 villages in the central district of Nigde province were used. In the study, the effect of year, dam’s age, gender and birth type on birth weight (BW), live weight on day 60 (LW1), live weight on day 120 (LW2), daily live weight gain (DLWG) on day 120 were investigated. The effects of year, dam’s age, gender and birth type on BW, LW1, LW2, DLWG were found to be statistically significant. While the effects of year, dam’s age and gender were found to be statistically effective on SR, the effect of birth type was found to be insignificant. In the study, fertility, fecundity and litter size in Akkaraman lambs were determined as 0.92, 1.03 and 1.13, respectively. As a result, remarkable progress has been achieved in Akkaraman lambs in terms of performance characteristics (LW1, LW2 and DLWG) between 2017 and 2021.

This experiment investigates the effect of flaxseed meal, rapeseed meal and vitamin E supplementation, when used together, on the production parameters of fatty acid composition and eggs quality characteristics in Tetra SL laying hens (38 to 46 weeks of age). For this, 120 hens were allocated one of three treatments, with 40 hens in each group. The hens were fed either a control diet (C), a control diet including 2.5 % flaxseed meal and 2.5% rapeseed meal (E1) or 2.5% flaxseed meal and 2.5% rapeseed meal with 73 mg/ kg feed of vitamin E as an antioxidant supplement (E2) for 8 weeks. Hens consuming the E1 diet had greater egg production and egg mass than those from group C. The feed conversion ratio in both the E1 and E2 groups was lower (P < 0.05) than in the C diet group. E2 had considerably higher egg weight, albumen pH, yolk pH and Haugh unit than E1 and C (P0.05), as a response to the vitamin E antioxidant effect. The most α-linolenic fatty acid content (1.07 g FAME) was found in eggs produced by chickens fed the E2 diet, (1.07 g FAME), followed by E1 (0.91 g FAME), with both being significantly higher than C eggs (0.23g FAME). Furthermore, all n-6 studied fatty acids concentrations were significantly lower (P < 0.05) in E1 and E2, while all n-3 fatty acids concentrations were significantly greater (P > 0.05) in E1 and E2. When comparing the n-6/n-3 ratio of fatty acids from experimental treatments (6.44 and 6.74) with C treatment (18.19), a significant difference was observed (almost 65% lower).