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Effect of Organic and Industrial Fertilizers on Reducing Sugar, Specific Gravity, Dry Matter and Starch Composition of Fresh Harvested Irish Potato Varieties in Musanze District Rwanda Full text
2024
Theophile Kanyarwanda | Sylvestre Habimana | Karemera Noella Josiane Umuhoza | Fabrice Musana Rwalinda | Alphonsine Mukamuhirwa
Potatoes for use in industrial processing must have a low reducing sugar concentration. This study investigates the impact of organic and synthetic fertilizers on the composition of Irish potato cultivars’ reducing sugar, specific gravity, dry matter, and starch. The research was conducted at Busogo Farm in Rwanda, using randomized complete block designs in seasons 2021 B and 2022 A with rate of ten tones per hectare under the doses of 100%, 50%, and 0% and three hundred kilograms per hector under the dose of 100%, 50% and 0% respectively for farmyard manure and NPK 17.17.17. Results showed significant effects of season and treatment on reducing sugar levels in potatoes. Twihaze and Kirundo varieties recorded high glucose (0.09%), while Gisubizo variety in control, recorded low glucose (0.01%). Additionally, the analysis of variance showed that Irish potato varieties with low to moderate and moderate to high glucose contents, respectively, experienced extremely significant (p 0.001) effects of farmyard manure and NPK on glucose content for the seasons 2021 B and 2022 A. The application of inorganic and organic fertilizers had a significant impact (p 0.05) on dry matter in seasons B and A, according to the analysis of variance. Additionally, the results demonstrated extremely significant (p<0.01) differences in specific gravity and starch in both seasons, with Kinigi variety having higher values of dry matter, specific gravity, and starch content in season B and A, respectively, of 24.58, 1.10, and 18.59%. Season 2022 A’s high decreasing sugar levels were caused by meteorological conditions brought on by the soil’s high moisture content as well as the maturity of the tubers. Due to its low level of reducing sugar, Gikungu, Kirundo, and Kinigi are excellent for chips whereas Twihaze variety is advised for boiling cooking methods.
Show more [+] Less [-]Histological Fixation Process and Fixatives Full text
2024
Tuğba Dağdeviren | Hatice Kübra Yolcu | Bülent Ünver
Tissue monitoring generally includes the stages of fixation, dehydration, clearing, hardening (infiltration), paraffin blocking/paraffin(emmeding), sectioning, water removal, routine staining, and mounting. Fixation is the basic and first step in the microscopic examination of tissues. The histotechnical process, which includes components such as detection, tissue tracking and staining, basically aims to capture and visualize the state of the relationships between tissue parts inside and outside the cell and various cells at a certain time as close as possible to the living state. Maintaining the natural structure of the tissue is important for the follow-up phase. The main feature of a good fixative should protect the sample and make the macromolecules insoluble without changing the chemistry of the sample studied and allowing it to be examined as closely as possible to its living state. In histological tissue analysis including light microscope and electron microscope techniques, an appropriate fixation method is selected for each study. Detection solutions are classified in terms of content. The most commonly used fixative in light microscopic follow-up procedures is 10% formaldehyde. For the electron microscope, the gluteraldehyde-osmium tetraoxide binary is widely used for fixation purposes. Gluteraldehyde acts more slowly and is more expensive than formaldehyde. Formalin is obtained by dissolving formaldehyde in water. In addition, the fixed samples can be stored in the solution for months. With a successful fixation process, the structural properties of the tissue are preserved and thus it is possible to examine the tissue as closely as possible. Thus, better quality sections are obtained from the tissue samples taken. For this reason, it will be more efficient to interpret well-fixed samples by photographing them. In this review, which was created by using various sources, the elements to be considered for an ideal fixation were determined and it was aimed to provide an overview of successful fixation for light microscope and electron microscope.
Show more [+] Less [-]Effect of Sorbitol Spraying on Chlorophyl, Total Phenolic and Flavonoid in Fragaria ananassa. Duch. cv. Albion Leaves Full text
2024
Beril Kocaman
Strawberry (Fragaria × ananassa Duch.) is one of the most widely consumed and cultivated fruits worldwide. Sorbitol plays a role in plant responses to many biotic and abiotic stresses. In this research, we intended to understand the effect of sorbitol spraying on the bioactive compounds of strawberry leaves. The application of sorbitol at different concentrations (0, 25, 50 mM and 75 mM) greatly improved strawberry characteristics such as total chlorophyll, chlorophyll a and b, carotenoids, and total phenolics. As sorbitol concentrations increased, chlorophyll a and chlorophyll b values increased in the samples taken during the fruiting period and higher values were obtained. The carotenoid content increased by approximately 189.49% and the total phenolic content increased by 30.85% in strawberry plants treated with sorbitol compared with the control. Supply of sorbitol decreased flavonoid content. The results indicate that sorbitol treatment has no inhibitory influence on the overall growth of strawberries. Among the biochemical parameters analyzed, chlorophyll, phenolic, and carotenoid contents increased, whereas flavonoid content decreased with sorbitol application.
Show more [+] Less [-]The Effects of Rearing Systems on Incubation, Egg Production and Quality Traits in Pharaoh Quails Full text
2024
Ahmet Uçar
This study was conducted to determine the effect of rearing quails in different rearing systems on egg production, egg quality characteristics, and incubation results. The 360 quails were distributed equally to 3 different rearing groups (cage, enriched cage, and floor) in a male:female mixed at the age of 3 weeks. The eggs were individually weighed and recorded one by one daily (5487 eggs) for 3 months. To determine egg quality and incubation characteristics, 750 eggs were broken, and 3284 eggs were incubated, respectively. The results showed that female quail in the floor group have lower body weight at 6 weeks old (177.19 g), and they reached sexual maturity (age of first egg laying) later (65.36 day, P<0.01), The lowest egg production (61.14%) occurred in the floor group during the 3-month egg production period (P<0.05). The lowest average egg weight (9.07 g) was determined in the floor group (P<0.05). It was determined that the eggs of quails raised in the enriched group had a rounder shape index because they have larger widths (P<0.01). While the highest average yolk height (11.24 mm) was determined in the enriched cage group (P<0.01), the highest averages of albumen length (72.41 mm) and lowest averages of yolk index (48.43%) were determined in the cage group (P<0.01). The lowest average values (113.17) in Haugh unit were detected in the floor group (P<0.05). The highest fertility rate (98.44%) was in Floor♂:Cage♀, the highest hatchability of fertile egg rate (94.67%) was obtained from Enriched♂:Cage♀, and the highest hatchability (89.10%) was obtained from Floor♂:Cage♀ pairing (P<0.05). The lowest fertility rate (88.00%) was obtained from the Enriched♂:Floor♀, the lowest hatchability of fertile egg rate (86.01%) was obtained from the Enriched♂:Enriched♀, and the lowest hatchability (75.62%) was obtained from the Enriched♂: Enriched♀ groups. As a result, it has been revealed that the effect of various rearing systems is different on egg production, egg quality traits, and hatching results in quails. Thus, it has been shown that different programs at the rearing period to be applied to the male and female quails can achieve better incubation results and reproductive success.
Show more [+] Less [-]Antifungal and Antioxidant Properties of Some Artificial Antioxidants, Generally Recognized as Safe Compounds and Nano-Oxides Full text
2024
Ayşenur Gürgen | Sibel Yıldız
In this study, the usage potential of some artificial antioxidants, generally recognized as safe (GRAS) compounds and nano-oxides solutions in wood preservation industry, was investigated. For this purpose, antifungal and antioxidant properties of solutions were determined. Erythorbic acid, ethoxyquin, potassium disulfide, sodium ascorbate, sodium erythorbate and Enginerring and Nature Sciences Faculty (TBHQ) were selected as artificial antioxidants; dehydroacetic acid, sorbic acid and sodium benzoate were used as GRAS compounds and nano MgO, nano CeO, nano ZnO, nano SiO2 and TiO2 were investigated as nano-oxides in this study. Three different concentrations (0.5%, 1.0% and 1.5% ) were prepared, and anti-fungal test were carried out. The brown rot fungus Coniophora puteana (Schumach.) P. Karst. (BAM Ebw. 15) was used for the anti-fungal test. Then antioxidant activity of the solutions were determined. Iron (III) ion reducing antioxidant power method (FRAP) was used to determine the antioxidant activity of solutions. All solutions at 1.5% concentration completely inhibited the growth of C. puteana fungus. The antioxidant activity of solutions was sorted as artificial antioxidants>GRAS compounds>nano-oxides, respectively. It was concluded that the tested substances can be used as impregnating agents in wood preservation.
Show more [+] Less [-]Monitoring Trends in Packaged Food Supply for Added Sugar and Sweeteners: Are We Jumping out of the Frying Pan into the Fire? Full text
2024
Murat Gürbüz | Selinay Demirel | Miray Nur Aykut | Esma Nur Erdoğan | Beyza Balcı | Gözde Özaslan
Excessive sugar intake can lead to poor health outcomes. The use of sweeteners is considered as a strategy to reduce added sugar consumption. The presence of sweeteners in food products has increased significantly in many countries. Therefore, the aim of this study was to identify the added sugars and sweeteners in packaged products. 1550 (57.9%) out of 2676 food products contained at least one added sugar. Confectionery and desserts (35.38±24.82 g/100 g), and snack foods (20.70±16.20 g/100 g) were the main categories containing the highest amount of added sugar. 229 (8.6%) out of 2676 food products contained various types of sweeteners. The most popular added sugar was sucrose (62.9%), while the most popular sweetener was sorbitol (28.2%). This is the most comprehensive study in the city center of Edirne province in Türkiye reporting on the types and frequency of added sugars and sweeteners in packaged products. This study offers monitoring to improve the legislation of Türkiye on added sugars and sweeteners used in the food supply.
Show more [+] Less [-]Improving Bitter Gourd Growth and Yield in Different Soil Environments by Combining Biochar and Inorganic Fertilizer Full text
2024
Md. Nazrul Islam | Mahbub Rabbani | Muhammad Abdul Malek | Md. Sohag Khalifa | Zillur Rahman | Nusrat Nawreen Orpa | Md. Abdul Mannan
A study using the assigned Complete Block Design with three replications was carried out at Hobigong, Bangladesh from January to August 2021 to investigate the impact of different combinations of organic and inorganic fertilizers on the growth and production of bitter gourd. The experiment employed five different combinations of organic and inorganic fertilizers, which are listed below: T1: 4 kg of organic fertilizer per plant, T2: 4 kg of organic fertilizer + 2 liters of cow urine + 2 liters of bc (biochar), T3: 2 liters of liquid bc (biochar)) + NPK (44 g urea + 43 g TSP (Triple Super Phosphate) + 77 g MoP (Muriate of Potash), T4: NPK (44 g urea + 43 g TSP + 77 g MoP), and T5: 4 kg of organic fertilizer + 2 liters of bc+ NPK (44 g urea + 43 g TSP + 77 g MoP) on three distinct soils in the villages of Kalonjhora, Madhabpur, and Jointure. The growth and yield of bitter gourd at different study locations were significantly affected by the application of both organic and inorganic fertilizers, according to the results. The results showed that the use of both organic and inorganic fertilizers had a significant effect on the development and yield of bitter gourd at the different study sites. Maximum germination rate (90%), at 45 DAS (days after sowing), longest plant (173.28 cm) at 100 DAS, maximum number, longest and heaviest fruit (6.30 number of fruits per plant, 15.51 cm and 82.50 g, respectively) were obtained from crops grown in Jointer village that were applied with 4 kg organic fertilizer + 2 liters bc + NPK (44 g urea + 43 g TSP + 77 g MOP). According to the previously described research, most development and yield characteristics of bitter gourd are improved when grown in a mixture treated with 4 kg organic fertilizer + 2 liters bc + NPK (44 g urea) + 43 g of TSP + 77 g of MoP.
Show more [+] Less [-]Evaluation of Enzymatic and Non-enzymatic Antioxidant Defense Responses of Durum Wheat (Triticum durum Desf.) in Coping with Boron Stress Full text
2024
Rıdvan Temizgül
Wheat, one of the world's most important agricultural products, plays a vital role in meeting the nutritional needs of our growing global population. However, arid and semi-arid regions face a potential threat from boron (B) toxicity. While boron is an essential nutrient for plant growth and development, its excessive presence can be toxic. It disrupts physiological processes, causing chlorosis and necrosis, ultimately leading to yield loss or plant death. Although B deficiency is a problem in the soils of many countries, Türkiye is one of the few experiencing B toxicity problems in its agricultural areas. This study investigated the physiological and biochemical responses of durum wheat to various B concentrations (0-20 mg L-1) under controlled air-conditioned cabin conditions. Durum wheat exhibited a decrease in chlorophyll content, phenolic content, and antiradical capacity at B doses exceeding 10 mg L-1. However, carotene content increased steadily with increasing B concentrations. The activities of antioxidant enzymes, including superoxide dismutase (SOD), ascorbate peroxidase (APX), and glutathione S-transferase (GST), increased up to a B dose of 15 mg L-1. Catalase (CAT) and glutathione reductase (GR) activities increased up to 10 mg L-1 B dose but decreased at higher B levels. Proline content increased tenfold up to a B dose of 10 mg L-1, indicating an attempt to mitigate stress. Conversely, malondialdehyde (MDA) accumulation increased continuously (approximately 150%) with increasing B doses, suggesting membrane damage. Despite being considered B-sensitive, this study demonstrated that durum wheat can effectively cope with B stress up to a B dose of 10 mg L-1 under controlled conditions. Beyond this threshold, physiological and biochemical changes indicate a decline in stress tolerance. Many osmoregulators, carotenes, alkaloids, flavonoids, tocopherols, phenolic compounds, non-protein amino acids, and several unidentified metabolites are activated to support antioxidant defense. The SOS pathway and the released ROS force gene regulatory systems come into play. Following these, the ROS released in the organism are neutralized, and ionic homeostasis and cellular stress resistance are achieved.
Show more [+] Less [-]Determination of Factors Affecting the Cumin Production Decision of Agricultural Enterprises: The Case of Konya Province Full text
2024
Fatma Çiftci
Turkey has vast agricultural lands and diverse climatic conditions, and different plant species can be cultivated. Especially, Konya province, located in the Central Anatolia Region, is an important agricultural centre in Turkey. Konya province, which has a polyculture production pattern, stands out in the production of medicinal and aromatic plants due to its favourable climatic conditions and soil structure. In Turkey, 26.33% of cumin, which is among the medicinal and aromatic plants, is produced in Konya province. This study aims to determine the factors affecting cumin production. For this purpose, 65 cumin producers determined by proportional sampling method were interviewed. The data obtained through these interviews were analysed by using the Analytic Hierarchy Process (AHP) method to determine the weights of the criteria affecting the cumin production decision of the agricultural enterprises. In the process of determining the criteria influencing the cumin production decision, the opinions of subject experts working in universities, public institutions and organizations and producers were taken. Among the criteria determined as yield, price, labour requirement, water requirement, ease of marketing, mechanisation, input costs, cultivation knowledge, soil structure and subsidies, the most important criterion was found to be price with 28.11%. Price criterion is followed by input cost 22.57%, water requirement 12.13%, yield 8.71%, cultivation knowledge 8.43%, subsidies 6.82%, ease of marketing 4.74%, soil structure 3.63%, mechanisation 2.54% and labour requirement 2.25%. It is thought that the solution of the mechanisation problem will make a significant contribution to cumin production as it will reduce the need for labour force.
Show more [+] Less [-]Molecular Diversity Analysis of in vitro and Irradiated Tomato (Lycopersicon esculentum Mill) Grew Under Salt Stress Expressed by SCoT and ISSR Markers Full text
2024
Eman Fahmy | Ahmed Abo Doma | Mohamed Adly | Gamal El-Metabteb | Osama Helmy | Ayman El-Fiki
Tomato buds of cv. Idkawy were cultured in vitro on solid MS medium with 0.2 mg-l BAP. The plantlets that were produced were exposed to different doses of gamma radiation, ranging from 100 to 200 Gy. Afterward, single pieces of nodes were cut and moved to a fresh MS medium with 0.2 mg-l of BAP. The gamma radiation caused a mortality rate of 18.75% to 52.5% among the explants. The surviving plantlets were then cut into single node pieces and transferred to an MS medium containing 0.2 mg-l of BAP, with added NaCl concentrations of either 50 or 100 mM. There was increased mortality of the vegetative buds on the explants with increased salt concentrations. It was shown that the all gamma radiation doses caused reduced the percentage of survival at saline levels. The genetic diversity was assessment by using ten primers for each SCoT and ISSR markers to six irradiated treatments grew under salt stress (100 Gy x 50 mM, 150 Gy x 50 mM, 200 Gy x 50 mM, 100 Gy x 100 mM, 150 Gy x 100 mM, 200 Gy x 100 mM). It was showed that the polymorphism percentage mean of SCoT marker (29.56%) is higher than the ISSR marker (26.78%). The average of PIC values for both markers SCoT and ISSR were 0.197 and 0.288 (PIC ˂0.5), as well as, MI values were 0.077 and 0.081, respectively. In contrast, when considering the number of alleles (Ne), Nei's genetic diversity (H), and Shannon's information index (I) parameters, it was observed that the greatest genetic variation was caused by the combined treatment of 200 Gy x 50 mM NaCl using the SCoT marker. On the other hand, with the ISSR marker, the highest induced genetic variation was seen with the combined treatment of 150 Gy x 50 mM NaCl. The obtained results demonstrate that SCoT marker was more accurate and efficient than ISSR marker for distinguishing and genetic variation analysis of irradiated tomato plantlets grew under salt stress. The relationships within treatments were estimated through cluster analysis (UPGMA) based on SCoT and ISSR analysis.
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