Gerçek zamanlı kantitatif polimeraz zincir reaksiyonu (q-PCR), hem gen ekspresyonu analizinde hem de rutin Deoksiribo Nükleik Asit (DNA) ölçümünde nükleik asit miktarının belirlenmesi için kullanılan ileri moleküler bir yöntemdir. Gıda ve yem ürünlerinde genetiği değiştirilmiş organizma (GDO) kalıntıları için etiketleme eşiğinin Japonya’da %5, Avrupa Birliği’nde ise %0,9 olduğu göz önüne alındığında, doğru bir ölçüm metodu şarttır. GDO bileşenlerinin tespiti, kesin miktar tayini ve besin matrislerinde eser miktardaki kalıntısının tespit edilmesi q-PCR’da mümkündür. Bu amaçla çeşitli q-PCR kimyasalları kullanılmaktadır. Bunlar; interkalasyon boyaları, primer bazlı kimyasallar ve prob bazlı kimyasallar olarak üç gruba ayrılmaktadır. Marketlerde GDO ürünlerinin artan sayısıyla birlikte, her örnek için gerçekleştirilen analiz sayısı ve bu nedenle analiz maliyetleri artmaktadır. Bunun için GDO çalışmalarında, GDO’ların varlığının miktarını belirlemede hızlı ve ekonomik olan uygulanabilir taramalar yapılabilmesi için geliştirilmiş tespit yöntemlerine ihtiyaç duyulmaktadır. Bu çalışmada, q-PCR kimyaları ekonomikliği, verimliliği ve uygulanabilirliği açısından karşılaştırılmıştır.

Gediz Havzası yaygın tütün tarımı yapılan toprakların verimlilik durumlarının incelenmesine yönelik yapılan bu çalışmada, 60 farklı noktadan 0-30 cm derinlikten alınan toprak örneklerinin bazı fiziksel-kimyasal özellikleri ile besin elementi içerikleri belirlenmiştir. Araştırma sonuçlarına göre topraklar; genel olarak kumlu-tın bünyeli, hafif alkalin reaksiyonlu, kireçli, organik maddece iyi ve tuzluluk yönünden herhangi bir tehlike içermemektedir. Yöre toprakları genel olarak değerlendirildiğinde, %94’ünde toplam azot %62’sinde alınabilir K yetersiz iken, %70’inde alınabilir P, %85’inde alınabilir Ca ve %56’sında alınabilir Mg yeterli bulunmuştur. Toprakların genel olarak alınabilir Fe (%83’ü) ve alınabilir Cu (%92’si) kapsamları yeterli iken alınabilir Zn (%86’sı) ve alınabilir Mn (%85’i) içerikleri yetersizdir. İncelenen toprakların fiziksel-kimyasal özellikleri ve besin elementleri arasındaki ikili ilişkilerde, pH-Elektriksel İletkenlik (EC), pH-kireç, pH-silt, pH-kil, pH-K, pH-Ca; EC-N, EC-P, EC-K, EC-Ca, EC-Cu, EC-Zn; organik madde (OM)-N, OM-P, OM-Ca, OM-Cu, OM-Zn; % kum-Fe; kil-K, Kil-Ca, Kil-Mg, Kil-Cu içerikleri arasında pozitif; pH-kum, pH-Fe, pH-Zn ve pH-Mn; OM-kum; % kum-kil, kum-silt, kum-K, kum-Ca, kum- Cu; kil-Fe içerikleri arasında önemli negatif korelasyonlar bulunmuştur. Besin elementleri açısından toprakların N-P, N-Ca, N-Cu, N-Zn; P-Cu, K-Mg, Mg-Fe, Mg-Cu, Fe-Cu, Fe-Zn, Fe-Mn, Cu-Zn, Cu-Mn, Zn-Cu, Zn-Mn arasında pozitif; Ca-Fe arasında ise güçlü negatif ilişkiler belirlenmiştir. Tütünde kalite özelliklerini doğrudan etkileyen azot, Zn ve Mn elementlerinin yöre topraklarında yetersiz olması nedeniyle yörede yapılacak gübreleme programlarında bu durumun göz önünde bulundurulmasında yarar vardır.

The aim of this study is to determine and evaluate the shopping centers with children-friendly design according to the use of families and children in the city of Konya. Our research can only be done at the “Kent Plaza” and “Novada” shopping center, where the official permission can be obtained. Although official writings were written from the other 2 major shopping centers in Konya, unfortunately, the survey was not allowed. In the study, a face-to-face questionnaire was conducted on the basis of volunteerism. The results of the questionnaires were evaluated and interpreted using MS Excel program. As a result of the study, it was found that the shopping malls that were surveyed were adequate for families with children. It has been determined that shopping centers are not only shopping and sightseeing places, but also places where families can have fun when they bring their children, they can spend time together, they can meet their needs, children playgrounds, children’s clothing stores, baby-sitting rooms, children’s toilets, , places suitable for children with disabilities. According to the results of the survey, it has been concluded that “Novada” Shopping Center has family and child friendly design in terms of interior design and variety of usage.

The purpose of this study was to investigate the thermal resistance of acid adapted and non-adapted stationary phase Escherichia coli O157:H7, Salmonella enterica Typhimurium and Listeria monocytogenes in pomegranate juice. In addition, the performance of generic E. coli was evaluated as an indicator. Non-adapted stationary phase cells were grown by incubating inoculated tryptic soy broth without glucose (TSB-NG) at 36±1°C for 18±2 hours. Tryptic soy broth with 1% glucose (10 g/l; TSBG) was used for acid adaptation. All media used for L. monocytogenes was supplemented with 0.6% yeast extract. After washing the cells with peptone, 5 ml of pasteurized pomegranate juice was added onto the pellet to obtain inoculated juice with a initial concentration of 107-1010 log CFU/ml. Inoculated pomegranate juice was sealed into the microcapillary tubes. Microtubes were heat treated in waterbaths at 50, 52 and 54±1°C by immersing at pre-determined time intervals. Survived populations were counted on tryptic soy agar (TSA). S. Typhimurium had the lowest thermal resistance in pomegranate juice. At 50°C, E. coli O157:H7 was the most resistant, whereas L. monocytogenes was more thermally tolerant at 52 and 54°C. Acid adaptation decreased the thermal resistance of E. coli O157:H7, but increased the heat resistance of L. monocytogenes at all tested temperatures significantly. Thermal tolerance of S. Typhimurium increased only at 50°C. The most resistant microorganism was non-adapted generic E. coli at 50 and 52°C; acid-adapted L. monocytogenes had the most thermal tolerance at 54°C. Thermal inactivation of microorganisms in pomegranate juice could be tested at lower temperatures compare to other fruit juices. This may be due to the natural antimicrobial effect and more acidic content of pomegranate juice.

Nowadays, due to some reasons, soil quality deteriorates, soil organic matter decreases, and plant growth is adversely affected such as damaging agricultural practices, unconscious pesticide and fertilizer use. There is also treatment sludge among the methods that will contribute to the soil. Sewage sludge, the inevitable by-product of municipal and other wastewater treatment plant operations, is a key issue in many countries due to its increasing volume and the impacts associated with its disposal. This environmentally hazardous treatment sludge can be significantly reduced by anaerobic digestion. However, the resulting gas and the residues from anaerobic digestion process need to be treated or stabilized. The use of sewage sludge in agriculture will provide both soil nutrient elements and waste sludge can be disposed with this application. Definitely, it is possible to evaluate the treatment sludges in many different areas. After cleaning the sludge from contaminants, it can be used as a good compost or soil improvement material. In this study, agricultural applications with sewage sludge were investigated.

Global warming, which has been increasing in recent years, is due to the increasing amount of greenhouse gases in the atmosphere. The agricultural sector, which has a 25% share in greenhouse gas formation, plays an important role in global warming. CO2, which has a share of 82% among greenhouse gases, is shown as the most important greenhouse gas. It is estimated that 10% of the CO2 in the atmosphere is missioned from the soil. When soil organic carbon, which is an indicator of soil fertility, is released into the atmosphere as CO2, N and S compound gases return to the earth and it causes inefficient soil as well as global warming. Organic carbon is a source of CO2 emissions when the soil is mismanaged. The organic carbon in the soil is transformed into CO2 with loosening of the soil and accumulates in the atmosphere, after it exposure to temperature and oxygen. Unconscious tillage and irrigations increase the physical and biological activity of the soil and cause CO2 emissions from the soil. CO2 emission, which is the main cause of drought, needs to be reduced with low cost, easy and practical applications. At the beginning of these applications, can be listed reduced tillage, deficit irrigation, reduction of fertilizer usage, reuse of agricultural wastes and agricultural management of wastewater. In this review, it is aimed to investigate CO2 emission effect of soil tillage depth and tools, exhaust gases of the vehicles used in soil tillage, the amount of irrigation water and irrigation methods, deficit irrigation, irrigation with wastewater, precipitation, plant factor, fertilization, properties and temperature of soil. In addition, approaches that can be applied to reduce CO2 emission are mentioned.

This study is consisted of data obtained from the survey conducted with 400 consumers in Uşak province urban area. As a result of the analysis, the average amount of annual per capita chicken meat consumed was calculated as 13.64 kg. The average amount of consumption increases due to the increase in income level. 39.2% of the consumers (consume chicken meat once a week. However, nearly half of consumers (41.7%) consume chicken meat, this is one third in total meat consumption. This ratio is changing according to income groups. Consumers supply chicken meat from vendors, grocery stores and groceries. The main reason for consumption of chicken meat for consumers; low price, easy to find on the market and healthy. Nearly half (42.5%) of the consumers prefer whole chick. The percentage of those consumed in the chicken meat consumed by the consumers is very low. Factors that are effective on consumers preferences for chicken meat; the freshness of the product, the price, the type of packaging, the quality, the producer company, the advertisement of the product, the taste of the consumer and the date of manufacture of the product. According to consumer preference (but, baguette, chest, wings etc.) processed products can be prepared according to the grill or other purposes and presented to the consumer. As consumers are prepared to extra payment for organic (ecological) food products, the farmers can be guided by incentives to organic poultry breeding systems.

Anoectochilus formosanus Hayata was demonstrated to have a benefit healthy due to containing active pharmaceutical ingredients. However, A. formosanus is usually processed to produce tea bags which would destroy the bioactive compounds because of the processing procedure. The aim of this study was to evaluate the influence of extracted methods including microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), and fermentation by Lactobacillus acidophilus ATCC-4356 to extract the active pharmaceutical ingredients from A. formosanus. The extracted liquid was analyzed total phenolics, total polysaccharide, and antioxidant activity. The results showed that three methods have a positive effect on the extraction of bioactive compounds of A. formosanus in which the fermentation showed the best result. The total phenolic content, total polysaccharide content and antioxidant capacity that extracted by the fermentation method were 11.762 mg GAE/g; 48.914 mg GE/g, and 1.582 mgVit C/g compare to MAE and UAE which were 7.818 mg and 8.128 GAE/g samples; 41.22 and 37.91mg GE/g samples; 1.032 and 1.163 mgVit C/g respectively. The A. formosanus fermentation method by L. acidophilus promotes bioactive compounds of high biological value. This study would suggest a novel use of lactic fermenting A. formosanus in the production of functional foods.

The data in this study was provided from 400 households in the Kucukcekmece District of Istanbul Province in 2011. Unclustered Proportional Sampling Method was used to determine the sample size of the study. The primary objective of this study was to identify the most suitable type of milk for consumers. In determining the most appropriate type, four criteria were taken into account. These criteria are hygiene, shelf-life, nutritional content, and price. Based on the data, milk consumption was 30.2 L per annum per capita in Istanbul Province. The households consist of about 3.7 individuals, and they give importance to hygiene by 26.4 percent, shelf-life by 26.7 percent, nutritional content by 29.6 percent and price by 17.2 percent. 55.3 percent of the households prefer UHT (long of shelf-life Tetra Pac packaged milk which heat-treated range of 135-150°C in a short period of 2-4 seconds) milk, 28.5 percent prefer Pasteurized milk, and 16.2 percent prefer Raw milk. Also, while households gave more importance to price and nutrient content at the raw and pasteurized milk, shelf life and hygiene content at UHT milk. Considering these criteria, the most suitable type of milk for the consumers was UHT milk. As a result, the national companies marketing pasteurized milk in the research area need advertisement works that inform the consumers regarding pasteurized milk.

The aim of this study was to evaluate the clinic-histopathologic characteristics and to compare the morphometric measurements of healthy and subclinical laminitic claws of dairy cattle at different ages and weights. Non-lame 60 Holstein feet randomly collected from the slaughterhouse were evaluated. The effects of age, body-weight, claw location (right front lateal or right front medial etc), and presence of laminitis were investigated. The claws’ conformation were evaluated morphometrically with ten measurements (toe length, toe height, outer and inner edges of the claw, heel height, the length of heel, the length of diagonal front wall, dorsal hoof angle, the width and the length of the sole). The claws were classified as normal or laminitic according to the histopathologic findings. The clinical findings of laminitis was confirmed on 71.2% of the claws (n=66). The toe length, toe height, the height of outer and inner edges of the claw, heel height, the length of heel, the length of diagonal front wall were smaller in laminitic claws. The dorsal hoof angle of healthy claws were bigger and statistically significant than the laminitic claws. Small haemorrhagic areas were determined in the parietal corium in the laminitic claws comparing to macroscopically healthy claws. The histopathologic characteristics of the corium of laminitic claws involve the hyperaemia, haemorrhages, oedema, thrombosis of capillaries and presence of mononuclear cell infiltration in dermis, stretching epidermal lamella, necrosis of epithelial cells and detachment of the lamellar basement membrane. According to this study results, contrary to literature, there was not a reliable relation between some changes in morphological structure of the claws and the presence of the laminitis were observed.