Recycling plant residues through various processes is essential for addressing waste issues in our country, because it contributes to the protection of the environment and ecosystems. Composting is one of the most important recycling methods for plant residues. Composts are not only natural, but they also enhance soil fertility and exhibit fungitoxic properties on the mycelial and spore germination of fungi. This study aimed to isolate bacterial bioagents from compost derived from various agricultural and household organic wastes, and to assess their antagonistic potential against important plant pathogens Botrytis cinerea and Sclerotinia sclerotiorum under in vitro conditions. A total of eight bacterial isolates were obtained, identified as belonging to Bacillus spp. Dual culture tests were used to evaluate the potential of these bacterial candidates to inhibit the mycelial growth of phytopathogenic fungi. In vitro trials revealed that six bacterial isolates exhibited varying degrees of antagonistic effects (23.3%- 63.3%) on the mycelial growth of B. cinerea. Among the tested bacteria, two isolates were effective against S. sclerotiorum, demonstrating antifungal activity ranging from 80% to 83.3%. The results indicate that composts provide a favorable environment for microorganisms with antagonistic potential, suggesting that these cultivation environments could play a significant role in the biological control of fungal pathogens in agriculture. In conclusion, this study contributes to the development of sustainable agricultural practices by ensuring a more efficient utilization of organic waste.

The development of high-yielding modern wheat varieties to feed the growing population has had a negative impact on the production of ancestral and landrace crops. The use of modern varieties, which are very deficient in vitamins, minerals, antioxidants, and flavonoids, has caused people to turn to old varieties due to health problems that arise over time. In this study, which aimed to determine the glutathione S-transferase (GST) enzyme activity of registered varieties and landraces, the differences between the protein values and GST enzyme activity values of wheat were found to be statistically significant. When protein values were analysed among wheat varieties, einkorn wheat had the highest value with 15.53 mg/ml, and KUNDURU-1149 had the second highest value with 13.52 mg/ml. The lowest protein values were found in wheat landraces. Lr-4 had the highest GST enzyme activity with 299.7 mmol/min/mg protein and Lr-10 with 265.3 mmol/min/mg protein. A negative and high correlation was found between wheat protein values and GST enzyme activity, and it was determined that landraces were prominent in terms of GST enzyme activity.

Nearly all of the apricot varieties grown in Malatya are dried apricots and the plantation areas in this region are expanding daily. Due to the impact of climate change, producers are growing apricots mostly under limited irrigation or even dry conditions. Therefore, it is essential to determine the drought resistance characteristics of the varieties commonly cultivated in this region. In this study, different irrigation levels of 100%, 75%, 50% and 25% of available water were applied to Hacıhalioğlu, Kabaaşı, Çataloğlu, Hasanbey and Soğancı apricot varieties. To evaluate the resistance of the varieties to drought stress and its relationship with physiological changes, chlorophyll a and b, carotenoids, total sugar, total starch and abscisic acid contents in the leaves were analyzed. A decrease in chlorophyll a and b, carotenoids, total starch values and an increase in total sugar and ABA values were determined due to the decrease in irrigation rates. In Kabaasi and Hasanbey varieties, which were observed as the most resistant to water shortage, chlorophyll a and b, carotenoids, total starch values were higher and total sugar content was lower at decreased irrigation levels. No difference was detected between varieties in ABA values. As a result of the observations in the drought resistance tests and physiological analyses, it was concluded that the most resistant varieties were Kabaasi and Hasanbey. Unfortunately, the most sensitive variety was the most widespread Hacihaliloglu. In addition, analyzing and evaluating the physiological changes occurring in apricot under drought stress will be useful in developing the most appropriate irrigation strategies for each variety and increasing water use efficiency. It may also be useful in cross-breeding studies to develop new drought-resistant varieties.

For protein evaluation of feedstuffs for ruminants, the Streptomyces griseus protease test offers a purely enzymatic approach to estimate ruminal protein degradation. This study was conducted to determine the enzymatic crude protein (CP) degradability of alfalfa, sainfoin, and common vetch hays, which are commonly used in ruminant nutrition. To estimate CP degradation, fifteen samples from each type of hay were incubated in vitro with a commercial protease extracted from Streptomyces griseus. The incubation was carried out for 1, 4, 24, and 48 hours in a borate-phosphate buffer at pH 8. Significant differences in CP degradability values were found among all three types of hay across all incubation periods. For all incubation periods, sainfoin had the lowest CP degradability values (P < 0.05), due to its high content of cell wall components and condensed tannins (CTs). For incubation periods longer than 1 hour, common vetch had the highest CP degradability values, followed by alfalfa and sainfoin, respectively (P < 0.05). As a result, the use of the protease enzyme extracted from Streptomyces griseus was confirmed as an effective method for estimating the CP degradability of selected legume forages in the laboratory, eliminating the need for animal testing. However, since plant proteins are often embedded within carbohydrate complexes, it is recommended that future tests consider the combined use of protease and carbohydrase, particularly for sainfoin, which is rich in cell wall components and condensed tannins.

This research aimed to examine the impact of resveratrol and catalase on the motility, plasma membrane integrity, acrosomal membrane integrity, and mitochondrial activity of Ankara buck semen following freeze-thawed process. In this study, semen samples obtained from four mature bucks were divided into four groups: control (C), resveratrol 500 µM/ml (R), catalase 50 IU/ml (CAT), and resveratrol 500 µM/ml + catalase 50 IU/ml (CATR). After dilution with Tris/egg yolk extender, the semen samples were frozen in liquid nitrogen and then thawed for assessment. The CATR group gave the highest values across all evaluated parameters (motility, plasma membrane integrity, acrosomal membrane integrity, and mitochondrial activity) compared to the other groups (61 ± 1.0%, 72.6 ± 0.70%, 70.73 ± 0.67%, 60.9 ± 0.79%, respectively) (p<0.05). In conclusion, the combination of catalase and resveratrol significantly improved the quality of buck semen after freeze-thawed process, thereby contributing to enhanced reproductive outcomes and genetic preservation.

Bu çalışmada, tahıl depolaması amacıyla farklı sac kalınlıkları ve destek elemanlarına sahip 85 adet silo modeli oluşturulmuştur. Silo boyutları,1500×3000 mm sac tabakalar kesilmeksizin kullanılarak çapı 4,77 metre ve yüksekliği (çatı hariç) 10,5 olarak belirlenmiş ve araştırma süresince sabit tutulmuştur. Çalışmanın temel amacı, çelik siloların maliyetinin büyük kısmını oluşturan yapı malzemesini azaltarak maliyeti düşürmek için optimizasyon yapmaktır. Oluşturulan modeller için kaynaklanabilirlik, işlenebilirlik ve maliyet yönünden uygun malzeme olarak St44 yapı çeliği seçilmiştir. Silonun stabilitesini artırmak ve olası yapısal sorunları minimize etmek amacıyla, NPU ve lama destek elemanları tasarıma dahil edilmiştir. Oluşturulan silo modellerinin yapısal analizleri ANSYS sonlu elemanlar yazılımı ile gerçekleştirilmiştir. ANSYS yazılımı kullanılarak yapılan analizlerde, her bir silo modeli için statik yükleme koşulları altında oluşan toplam deformasyon, gerinim ve gerilme değerleri hesaplanmıştır. Ayrıca, her bir tasarımın toplam ağırlığı da değerlendirilmiştir. Bu analizler sonucunda elde edilen veriler karşılaştırılarak, en uygun performans ve maliyet dengesini sağlayan optimum silo tasarımı belirlenmiştir. İmalat sırasında sac kalınlığını artırmadan, yeterli destek elemanlarının kullanılması ile genel ağırlığın %58 oranında azaltılabileceği tespit edilmiştir. Bu bulgu, imalat sırasında malzeme maliyetlerini önemli ölçüde düşürmekte ve yapısal performansı koruyarak ekonomik bir çözüm sunmaktadır.

The escalating global population, diminishing agricultural lands, and the overarching global climate crisis are significantly impacting pepper cultivation. These challenges exacerbate the vulnerability of pepper plants to various biotic and abiotic factors, particularly viral diseases, resulting in diminished yield and quality. Cucumber mosaic virus (CMV), a notable concern for peppers in the Solanaceae family, is causing substantial quality and yield losses, with no effective chemical control methods currently available. This study focuses on exploring the genetic structure of CMV isolates obtained from pepper production areas in Kilis province and comparing these regions through the High-Resolution Melting (HRM) method. CMV isolates, cultivated in tobacco plants, have their partial coat protein sequences compared with those of other CMV isolates registered in the gene bank. The nucleotide sequences of identified CMV isolates are phylogenetically grouped and compared using an HRM graph. The HRM graph effectively distinguishes Kilis 3 and Kilis 4 isolates, clustered similarly to sequence patterns, from other isolates. The study highlights the utility of HRM analyses in identifying differences between isolates before determining sequence patterns. In the gene bank comparison, Kilis CMV isolates distinguished from others. Similarities were observed with isolates from Iran's Balsam (Impatiens balsamina - LC066478), Türkiye's Radish (Raphanus sativus - LC0665051), and Wild Turnip (Rapistrum rugosum - LC066514, LC066511, LC066517). The study found that Kilis 7 CMV isolate, transferred mechanically to 24 different pepper genotypes (C. annuum) from the local population, revealed susceptibility to CMV in the 24 lines developed from the Elbeyli population in Kilis province.

Optimum condition at 64.80% maize flour, 20% groundnut paste and 13.20 % palm oil was formulated to produced nutritionally enhanced aadun snack. The snack was stored in the different storage materials namely, sweet prayer plant leaves (control) which is usually used by most locals, low density polyethylene (LDPE), high density polyethylene (HDPE) and food grade plastic container (PC). The initial properties (energy, oxidative and sensory properties) of the enhanced aadun (before storage) were investigated and stored in each of the storage materials. The enhanced aadun samples in each storage material were analysed at two weeks interval for eighteen weeks. The results obtained were analysed statistically to examine the effect of the storage material on the aforementioned properties. The results for energy content decreased significantly (P>0.05) in across all the samples stored. The free fatty acid, acid valve and peroxide value increased significantly (P<0.05) in all the storage materials during the storage period but only the samples stored in PC and HDPE were within the recommended limit of FAO (Food and Agricultural Organization) at the end of the storage period. The sensory quality of the control sample was acceptable up to 12 weeks while samples in other storage materials were still acceptable at the end of the storage period under ambient storage condition.

İnsanların beslenmesinde gluten içeren besinler önemli yer tutmaktadır. Gluten metabolizması bozukluğuna bağlı oluşan hastalıklar bulunmakta olup bunlar çölyak hastalığı, buğdaya karşı IgE aracılı alerji ve çölyak dışı gluten duyarlılığı olarak sınıflandırılabilir. Bireylerin gluten duyarlılıkları arasında farklılıklar olup gluten duyarlılığı olan bireylerin gluten içeren besinleri tüketmeleri durumunda en başta bağırsak dokusu olmak üzere birçok dokuda histopatolojik yapılarında ve otoimmun sistemlerinde önemli değişiklikler meydana gelebilir. Dokuların histopatolojik yapısındaki değişiklikler dokulara göre değişmektedir. Otoimmun sistemdeki değişiklikler ise Transglutaminaz, Gliadin, IgA, IgG, CD4 ve CD8 gibi parametrelerden en az birinin veya birkaçının incelenmesi ile tespit edilebilir. Gluten metabolizması bozukluğuna bağlı oluşan hastalıklarda etkili tedavi şekli glutensiz/gluten kısıtlı diyet olmakla birlikte bağışıklık sisteminde rol alan mekanizma ve moleküllerin saptanması yeni tedavi yöntemlerini oluşturulmasına yol gösterebilir. Bu çalışmada amaç gluten ve gluten metabolizması bozukluğuna bağlı oluşan hastalıklar arasındaki ilişkinin derlemesidir.

Zorunlu hücre içi bakteri olan Coxiella burnetii, insanlar da dahil olmak üzere omurgalıların zoonotik bir hastalığı olan Q ateşinin etkenidir. Yaygın enfeksiyon yolları kontamine ahır tozunu solumak ve enfekte hayvanların atıklarıyla temastır. Bu çalışmanın materyalini Haziran-Eylül 2019 tarihleri arasında Türkiye’nin doğusundaki Van ilinde bulunan 70 sokak köpeğinden toplanan 200 adet kene oluşturmuştur. Toplanan keneler %70’lik alkol içeren tüplere alınarak parazitoloji laboratuvarına nakledilmiştir. hangi etiketlerin yapıştırıldığı. Keneler tüplere yerleştirildi ve sıvı nitrojen ile dondurularak ezildi. DNA, kit üreticisinin protokolüne göre izole edildi. Coxiella burnetii’nin DNA’sını tespit etmek için IS1111 tekrarlayan transpozaz gen bölgesine özel bir Trans 1, Trans 2 primer çifti kullanıldı. Saflaştırılmış amplikonların çift yönlü dizi analizi, DNA dizileyici ile gerçekleştirildi. IS1111 transpozaz genini hedef alan PCR sonucunda 200 kenenin 2’sinde (%1) Coxiella burnetii pozitif sonuç elde edildi. Van ilinde serbest dolaşan köpeklerde Q ateşi epidemiyolojisinde potansiyel risk faktörleri ve kenelerin önemi, parazit kene türleri ve köpeklerde C. burnetii pozitif kenelerin prevalansı belirlenerek vurgulanmıştır.