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Acute and Subacute Toxicity of Ruta Montana Extract to Female Rats: Effect on Liver, Kidneys and Ovaries Full text
2023
Mahdeb Nadia | Khadidja Attafi | Souha Bouhouhou | Allouni Rima | Abdelouahab Bouzidi
Ruta montana L. is an annual aromatic plant of the family rutaceae. Quantitative analysis of the methanolic crude extract of Ruta montana L. yielded 8.43%, whereas the qualitative analysis revealed the presence of alkaloid or coumarin. The Litchfield and Wilcoxon method calculated the LD50 of the crude methanolic extract of Ruta montana L. in Wistar albino female rats at 393.18 mg/kg. This allows the plant to be classified as moderately toxic. The subacute toxicity study of the methanolic crude extract of Ruta montana L. in female Wistar albino rats treated with 100 mg/kg (≈ 1/4 LD50) and intraperitoneally showed a significant increase in body weight of the rats treated at the 4th week. Animals treated and sacrificed after 30 days showed a disturbance of the relative mass of the organs. Biochemical parameters of hepatic function assessment showed a significant increase in PAL with elevation of AST and ALT, whereas those of renal function revealed a significant decrease in creatinine with an increase in urea. Hematologic parameters recorded a decrease in RBC, HGB and HCT. The histological sections of the treated rats reveal the existence of blood congestion in the central veins and liver tissues, foci of necrosis and steatosis in the liver, blood congestion and some glomerular atrophy in the kidneys, as well as blood congestions and developed follicles without oocytes in the ovaries.
Show more [+] Less [-]Farmers' Views on Organic Grape Production in Adıyaman Province: Method Adoption and Problems Full text
2023
Aybüke Kaya | Songül Salık
Healthy life in a sustainable environment is possible with accessible food production. The Covid-19 epidemic is a serious threat worldwide. This epidemic has revealed the importance of agricultural products. One of the most important areas of the epidemic affecting the whole world is exports; however, increased demand for agricultural products and exports in the epidemic process in Turkey. Turkey has a say in the trade of seedless dried grapes in the world. Seedless dried grape is on the healthy products list of the WHO. In 2019/2020, it had a share of 36% of world exports (with 246 thousand tons of export). This study was performed to evaluate their thoughts and farmers’ problems with the adoption of organic farming in Besni district of Adıyaman. The research conducted in-depth interviews with the farmers producing organic grapes. Also, a face-to-face survey was conducted with 50 farmers. According to the findings, the land width of the farmers is not much. It has an average growing area of 37.52 decares. An average yield of 1.808 kg da-1 is obtained from this area. Serious differences have been found between conventional and organic farming. Moreover, government supports, high product prices, income, health, sustainability, and environmental protection are reasons farmers switch to organic farming. However, farmers argue that organic farming is less costly than conventional farming. Access to chemicals and marketing are major problems. As a result, farmers should be informed about organic farming. Additionally, it is thought that these studies will increase the productivity and product quality of the farmers. It is predicted that it will prevent rural to urban migration in the region.
Show more [+] Less [-]Comprehensive Evaluation of the Clinical Efficacy of an Anti-Diabetic Polyherbal Formulation Full text
2023
Hasib Sheikh | Md. Khairul Alam | Md. Abdus Samad
Background: There are several clinical studies examining the health advantages of several single medicinal herbs utilized in traditional blood glucose-lowering treatments. But very few or no studies on herbal formulations were made as Polyherbal for the same goal. As a result, it is now necessary to confirm that patients with hyperglycemia can benefit from such Polyherbal medicines as Dolabi. Methods: This prospective open-label, herbal coded test drug-controlled, randomized trial was conducted at the Munshiganj and Dhaka area in Bangladesh. We enrolled 108 male and 104 female patients of 30-70 years with primary and moderate hyperglycemia. They were recruited from the OPD of an Unani & Ayurvedic hospital in Munshiganj and different Unani clinics in Dhaka, Bangladesh after fulfilling the inclusion criteria. Patients were randomly assigned to receive metformin hydrochloride 500 mg two times daily and 2 tablets of Dolabi two times daily by using a random numbers table with the help of an assistant. Blood samples, height, weight, blood pressure, and personal data were recorded—laboratory results were obtained at the study baseline, after 1.5 months and after 3 months of intervention. Results: In the case of the test drug, results showed a significant decrease in blood glucose level between the baseline and after 3 months, in males, it was from 9.83±1.17 to 7.72±1.06 mg/dL for fasting glucose, from 16.60±2.35 to 8.23±1.17 mg/dL for 2 hours PP glucose, from 9.33±1.17 to 7.45±2.03 percent for HbA1c and for Insulin it reduces from 183.10±27.59 to 168.10±29.59 pmol/ L. The control drug metformin hydrochloride also showed a significant decrease in blood glucose level between baseline and after 3 months, in the case of males it was from 9.99±2.52 to 6.97±1.76 mg/dL for fasting glucose, from 17.43±5.05 to 7.89±2.42 mg/dL for 2 hours PP glucose, from 10.43±2.36 to 6.87±1.18 percent for HbA1c and for Insulin it reduces from 198.75±30.61 to 183.75±30.61p mol/L. In the case of females the test drug showed a significant reduction in fasting glucose, 2 hours PP glucose, HbA1c and Insulin between the baseline and after 3 months, it was from 10.02±1.11 to 7.78±0.93 mg/dL, from 16.88±2.21 to 8.16±1.11 mg/dL, from 9.84±1.04 to 7.45±1.03 percent and from 199.47±30.90 to 173.47±30.90 mg/dL respectively. In the case of females, the control drug showed a significant reduction in fasting glucose, 2 hours PP glucose, HbA1c and Insulin between baseline and after 3 months, it was from 10.18±1.92 to 6.71±1.59 mg/dL, from 18.70±3.88 to 7.60±3.74 mg/dL, from 10.58±1.08 to 6.98±1.08 percent and from 200.00±31.83 to 188.00±31.83 mg/dL respectively. Conclusions: We can infer the following from the present study’s findings: The polyherbal formulation Dolabi is able to reduce the blood glucose level. It can be an effective drug for primary hyperglycemic patients.
Show more [+] Less [-]Nutritional Composition and Apparent Metabolizable Energy (AME) Value of Black Soldier Fly Larvae (Hermetia illucens L.) Full-Fat Meal for Broiler Chickens Full text
2023
Dumeshi Ellawidana | Ruvini Kamalika Mutucumarana | Darshanee Ruwandeepika H.A. | Manjula Sumith Magamage
Black soldier fly larvae (BSFL; Hermetia illucens L.) is a proven high-cost protein source replacer and could be grown in a range of bio-degradable waste materials where hardly incorporated into broiler diets locally. The present study was aimed to assess the nutritional composition of BSFL, and apparent metabolizable energy (AME) value of BSFL meal provided to broiler chickens. BSFL full-fat meal produced from kitchen waste as a substrate were examined for their proximate composition, minerals and fatty acid profile. Eighty, 21-d old unsexed Cobb-500 broiler chickens (BW±SD: 665.8 ±14.3 g) were assigned randomly into 16 battery cages (04 replicates, five birds/replicate). A maize-soybean meal-based diet was used as the basal diet which was partially substituted by pre-analyzed BSFL meal at the rates of 5%, 10%, and 15% to produce three test diets. Birds were fed in a completely randomized design for 7-d with a 4-d adaptation period. Excreta were collected for three days from day 25 to 28. The results envisaged that the crude protein (CP) and ether extract (EE) contents of the kitchen waste were 12.3%, and 10.5%, respectively. BSFL meal when analyzed had 34.4% CP and 47.3%, EE. The fatty acid (FA) profile of the kitchen waste was more or less similar to that of BSFL‘s meal. The estimated AME of the BSFL full-fat meal fed for broilers was estimated to be 15.7 MJ/kg. The BSFL full-fat meal can be utilized sustainably in feed formulation and has a high potential to replace costlier feed ingredients.
Show more [+] Less [-]Polygalacturonase Production by Sarocladium strictum T4 Isolate using Apricot Pulp as Substrate in Non-Sterile Culture Conditions Full text
2023
Ruhşen Aydın Karaağaç | Mehmet Nuri Aydoğan
In this study, 34 molds showing pectin degradation activity were isolated from the soil of orchards, by several tests. By using these isolates, pectinase group enzymes; studies on pectin lyase and polygalacturonase production were performed in the batch system and under non-sterile culture conditions. 5 isolates denoting polygalacturonase activity were coded as: T1, T2, T3, T4, T5 and the best polygalacturonase activity among these isolates was determined as 354.4 U/L in T4 isolate. Thus, optimization analyses continued by the use of this isolate. Initial apricot pulp concentration, temperature, pH and incubation period were tested as optimization parameters. The effects on enzyme activities were investigated by changing the initial apricot pulp concentration in the range of 5-100 (g/L), and in this regard, polygalacturonase activity was determined as 397.4 U/L at 50 g/L. In addition, the pH parameter was analyzed in each unit between pH = 3 – 8, and the temperature was tested by increasing 5 units in the range of 5-25°C. Consequently, the maximum polygalacturonase activity was determined as 405.7 U/L at pH 5 and 406.3 U/L at 15°C. Besides, the effect of the incubation period was studied within 1-5 days and the maximum polygalacturonase activity was determined as 429.0 U/L on the 4th day (after 96 hours). As a result, the above-mentioned T4 isolate, with which the optimization studies were conducted, was identified as Sarocladium strictum (Top ekinküfü) T4 by molecular methods.
Show more [+] Less [-]Effect of Time and Temperature Storage on the Quality of unpasteurized Prickly Pear Juice Enriched with Hydro-soluble Opuntia ficus indica seeds Extract Full text
2023
Amira Sakhraoui | Noureddine Touati | Soraya Hihat
This study aimed to evaluate the effect of incorporating hydrosoluble Opuntia ficus indica seeds extract in unpasteurized prickly pear juice and monitoring its stability. For this purpose, titratable acidity (TA), total soluble solids (TSS), browning index (BI), total phenolic compounds (TPC), total flavonoids (TF), antiradical activity (DPPH), ferric reducing antioxidant power (FRAP) and microbial analysis were monitored for both enriched and controlled juices during different time and temperature storage. Before storage, the enriched juice values were respectively 0.096±0.001%, 14.1±0.01%, 0.756±0.01, 133.3±3.4mgGAE/100ml, 5.58±0.07mgQE/100ml, 95.89±14.27mgGAE/100ml and 59.34±5.52mgGAE/100ml for TA, TSS, BI, TPC, TF, DPPH and FRAP; while 0.16±0%, 14.1 ±0.001%, 1.2±0.01, 88.39±4.2mgGAE/100ml, 3.98±1.003mgQE/100ml, 51.08±14.27 mgGAE/100ml and 50.33±5.16mgGAE/100ml for the control juice. The microbial analysis revealed the absence of microorganisms even the juices were unpasteurized. Moreover, the results revealed that the enrichment attenuated significantly the effect of storage; indeed, the use of the prickly pear seeds extract in combination with the juices can be a good alternative to enhance the shelf life of unpasteurized prickly pear juice, and improve their quality attributes as well as to minimize the unwanted changes in the nutritional and organoleptic properties.
Show more [+] Less [-]Carpinus orientalis Mill. Fidanlarının Kalite Özellikleri ve Morfogenetik Varyasyonlar Full text
2023
Ebru Atar | Fahrettin Atar | Deniz Güney
Çalışmada, farklı yükseltiden temin edilen tohumlardan yetiştirilmiş 1+0, 2+0, 2+1 ve 2+2 yaşlarındaki doğu gürgeni (Carpinus orientalis Mill.) fidanlarının bazı morfolojik özelliklerinin belirlenmesi, morfolojik özelliklere bağlı olarak varyasyonların ortaya koyulması ve gürbüzlük indisi değerlerine göre kalite sınıflarının tespit edilmesi amaçlanmıştır. Materyal olarak doğu gürgeni türünün doğal yayılış alanı olan Trabzon-Maçka havzasında deniz seviyesinden 1200 m’ye kadar olan üç farklı yükselti basamağından (Maçka-1, Maçka-2, Maçka-3) toplanan tohumlardan yetiştirilmiş fidanlar kullanılmıştır. Farklı yaşlardaki fidanlarda kök boğaz çapı ve fidan boyu değerleri ölçülmüştür. Ayrıca fidanlara ait ölçülen morfolojik karakterler ile gürbüzlük indisi değeri hesaplanmış ve buna göre fidan kalite sınıflandırması yapılmıştır. Çalışma sonucunda ortalama fidan boyları fidan yaşının artmasına bağlı olarak sırasıyla 20,11 cm, 36,85 cm, 55,31 cm ve 82,74 cm olarak elde edilmiştir. Fidanlara ait ortalama kök boğaz çağı değerleri ise fidan yaşının artmasına göre sırasıyla 2,98 mm, 6,26 mm, 8,68 mm ve 12,08 mm olarak belirlenmiştir. Yapılan varyans analizi sonucunda ölçülen parametreler bakımından populasyonlar arasında istatistiksel olarak anlamlı farklılıkların olduğu tespit edilmiştir. 2+0 yaşlı fidanlar haricinde diğer yaştaki fidanlarda ölçülen morfolojik parametrelere ilişkin en yüksek varyasyon katsayıları genel olarak en yüksek rakımda yer alan Maçka-3 populasyonunda elde edilmiştir. Fidan yaşının artmasına paralel olarak kök boğaz çapı ve fidan boyu arasındaki korelasyon katsayısı değeri genel olarak azalmıştır. Gİ değerlerinin yükseltinin artmasına bağlı olarak genellikle azaldığı ve yüksek rakımlı populasyonların gürbüzlük indisine göre daha kaliteli fidanlara sahip olduğu belirlenmiştir.
Show more [+] Less [-]Comparative Fatty Acid Compositions of Tissues of Rainbow Trout (Oncorhynchus mykiss) with Different Ploidy and Sex Full text
2023
Birol Baki | Dilara Kaya Öztürk
The purpose of this study was to evaluate the fatty acid contents in various tissues (fillet, liver, gonad) of different ploidy (triploid and diploid) and sex (female and male) rainbow trout (Oncorhynchus mykiss) in the breeding season. In the study, diploid and triploid rainbow trouts belonging to the same age group (3+) were used. Fish were fed with commercial feed containing 45% crude protein and 20% crude fat until satiation. At the end of the 75-day study, biometric measurements of the fish were made and the tissues were stored in a deep freezer until biochemical and fatty acid analysis. The first finding of this study identified that ploidy (triploid and diploid) affects the biochemical and fatty acid composition of rainbow trout. The second major finding was that the polyunsaturated fatty acid values were higher and the saturated fatty acid values were lower in all tissues (especially female gonads) than other fatty acids. The results also indicate that the comparative among the biochemical and fatty acid composition of the fillet, liver, and gonad of rainbow trout is further illuminated by these data.
Show more [+] Less [-]Determination of Production and Marketing Behaviors of Producers Selling Products to the Turkish Grain Board, (Kırşehir Province Mucur District Micro Field Study) Full text
2023
Hasan Gökhan Doğan | Aybüke Bulut
The aim of this study is to examine the socio-economic characteristics of producers who sell wheat to the Turkish Grain Board (TGB), which is an interventionist organization in the purchase and sale of grain in Türkiye, as well as some of their behaviors regarding production, marketing preferences, and their relations with TGB. The sample of the study consisted of 100 wheat producers in the Mucur district of Kırşehir province. The obtained data were interpreted by converting them into tables, cross-tabulations, and graphs. The data in the cross tables were statistically interpreted using Chi-square analysis. It can be said that producers are not satisfied with TGBs’ purchasing practices for some reasons. The most important reason for this is that quality-based purchasing practices have an extremely negative impact on prices. It was determined that they were not satisfied, and this resulted in high price reductions in quality-based purchasing, and as a result, there was distrust in analysis practices. Quality-based purchasing policy is a method that allows wheat quality characteristics to be determined with various devices in a short time. This method, which is decisive in quality classification and product pricing scale, must be explained correctly to producers. Otherwise, the producer ’s trust in the TGB may gradually decrease. This can be achieved through initiatives taken by the institution or through various publications and training activities. One of the most important results of this study is that TGBs and decision-makers are more sensitive to local producers. For decision-makers to maintain their influence on the producer, they must carefully examine the details of the processes with an inductive approach, starting from the bottom up. This situation is considered critical in terms of food security.
Show more [+] Less [-]The Comparative Analysis of a Developed Swing Beater and Conventional Beater of a Palm Nut Cracking Machine Full text
2023
Oluwatobi Okunola | Olawale John Olukunle | Oluwafemi Adeyemi Adetola | Waleola Akinfiresoye
Based on high dependent of many processing company on palm kernel oil, high quality palm nut cracking machine is not only necessary but also important to revitalize the production of palm kernel in other to meup with ever increasing industrial demand. Different palm kernel beaters; Swing beater (SB) and the conventional type known as rigid beater (RB) of an existing palm kernel cracker were investigated using the moisture content (7, 17, 26% (db)), five different speeds (970, 1200, 1450,1750, 2430rpm) and three different average nut sizes (14.5, 22.15, 29.43mm) of palm kernel nut. Approximately, six thousand palm nuts of Tenera specie were collected, dried, cleaned and sorted to evaluate the machine. Result shows that, the maximum quality efficiency recorded for Swing beater was 89.5% at 17% moisture content (db), 29.4 mm average nut size and 970 rpm machine speed. Similarly, for the performance of rigid beater, the maximum quality efficiency of 71.5% was recorded at 26% moisture content (db), 29.4 mm average nut size and 970 rpm machine speed. The effect of nut size was however not significant on the quality efficiency however it was generally observed that quality efficiency increased with increase in the nuts size. The machine speed has a significant effect on the quality efficiency. Generally, the quality of the kernel recovered decreased as the machine speed decreased. Beater configuration has significant effect on the quality efficiency of the palm nut cracking machine at 5% significant level. Swing beater can be used instead of conventional beater (rigid beater) to crack palm nut for higher quality of whole kernel recovery.
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