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Protective Effect of Vitamin C (Ascorbic Acid) Supplementation on Post-Thaw Motility and Fertility of Cryopreserved Rainbow Trout (Oncorhynchus mykiss) Sperm
2020
Uğur Yavuz | Yusuf Bozkurt
Ascorbic acid (Vitamin C) is one of the important antioxidants, which naturally present in seminal plasma of fish. On the other hand, whether its effect may improve sperm quality following cryopreservation process still remains its uncertainty. Thus, the present study aimed to analyse the effect of different extenders supplemented with different ascorbic acid concentrations on post-thaw motility and fertility of frozen-thawed rainbow trout (Oncorhynchus mykiss) sperm. Selected sperm samples were pooled and diluted at 1:3 ratios with two different extenders (E) composing such as (E-1) 300 mM glucose, 10% egg yolk and 10% DMSO and (E-2) 0.6 mM sucrose and 10% DMSO. Each extender was supplemented with vitamin C at 1, 5 and 10 mM concentrations. Following dilution, the sperm was loaded into 0.25 ml straws and frozen in liquid nitrogen vapour. The straws were then plunged into liquid nitrogen for storage. Fertilization was carried out using the dry fertilization technique. Highest post-thaw motility (50±5.77) and fertilization (56±1.00) results were obtained with the extender-1 (E-1) containing 10% DMSO concentration. In conclusion, the present study indicated that addition of ascorbic acid to the extenders improved rainbow trout sperm motility resulting higher fertilization of the eggs.
Show more [+] Less [-]Effect of Different Media on Short Term Storage of Leaping Mullet (Liza saliens (Risso, 1810)) Sperm
2020
Serhat Engin | Muammer Kürşat Fırat | Osman Özden | Cüneyt Suzer | Müge Hekimoğlu | Onurkan Antepli | Şahin Saka
The present study aimed to evaluate the activation characteristics of the leaping mullet spermatozoa and diluted with four different media (D1, D2, D3, D4) for 250 h at 4±2°C. The semen was collected from nine wild fish in Homa Lagoon, İzmir, Turkey in July, during reproductive season. In each trial (n = 9), semen samples were collected and mixed to reduce sperm quality variation to obtain the required gametes for the experiment. The semen was activated with natural seawater (salinity of 34, pH 7.9 and 4±2°C) to determine: motility time, motility rate and viability of sperm. Dilution with inactivation media 1:3 increased motility time of diluted semen than control group. At the end of the experiment, no significant difference was found for the duration of survival in the D2 and D4 groups. The highest survival rate was found in D2 group.
Show more [+] Less [-]Herbisit Uygulamaları Yapılan Amaranthus retroflexus L. Bitkilerinden Elde Edilen Tohumların Çimlenme Özellikleri
2020
Oğuzhan Cerit | Derya Öğüt Yavuz
Şeker pancarı yetiştiriciliğinde Amaranthus retroflexus L. önemli yabancı otlardan biridir. Çalışmada, ekim öncesi chloridazon (C), çıkış öncesi ve sonrası metamitron (M) ile çıkış sonrası ethofumesate + phenmedipham + desmedipham + lenacil (EPDL) etkili maddeli herbisitlerin ve bazı kombinasyonlarının uygulanması sonucu hayatta kalan A. retroflexus bitkilerinden toplanan tohumların çimlenme kapasitelerinin belirlenmesi amaçlanmıştır. Toplam çimlenme oranı (%), normal/anormal çimlenme oranı (%), ortalama çimlenme zamanı (gün) ve tohum ağırlığı (g) parametreleri belirlenmiştir. Sonuç olarak; chloridazon + ethofumesate + phenmedipham + desmedipham + lenacil 2, chloridazon + metamitron (çıkış sonrası) ve metamitron (çıkış öncesi) + metamitron (çıkış sonrası) kombinasyonlarına maruz kalan A. retroflexus bitkilerinin uygulama yapılmamış bitkilerden toplanan tohumlara kıyasla çimlenme özellikleri önemli ölçüde etkilenmiştir. Çimlenme özellikleri bakımından, en düşük toplam çimlenme oranı %85 ile metamitron (çıkış öncesi) + metamitron (çıkış sonrası) kombinasyonundan elde edilmiştir.
Show more [+] Less [-]Effects of Drought and Temperature Stress on Germination and Seedling Development of Sunflower
2020
Nurgül Ergin | Mehmet Demir Kaya
This study was aimed to determine the responses of some sunflower hybrids to drought and low temperature stresses during germination and seedling development period. Sunflower hybrids of Sanbro MR, 63LE113, 64LL62 and Meteor were used in the study. Germination percentage, mean germination time, root and shoot length, root / shoot ratio, seedling fresh and dry weight of the sunflower hybrids were investigated at two temperatures (low 15°C and optimum 25°C) and drought stresses (distilled water, -1.5, -3.0, -4.5 and -6.0 bar) induced by polyethylene glycol 6000. The results of the research showed that there were significant differences among the sunflower hybrids in terms of the investigated characteristics. Increased drought stresses led to decreasing germination percentage, root and shoot length, seedling fresh weight, but caused a prolonged germination time and increased seedling dry weight. No significant change was observed in the germination and seedling development of the sunflower up to drought of -3,0 bar. The root / shoot ratio of sunflower hybrids increased at 15°C. The Sanbro MR had the fastest and highest germination along with the highest seedling fresh and dry weight at low temperature and higher drought stresses. It was concluded that sunflower hybrid Sanbro MR gave better performance under drought and low temperature conditions than the other hybrids.
Show more [+] Less [-]Algae and Macrophytes Flora of Karamenderes Stream (Çanakkale, Turkey)
2020
Hüseyin Erduğan | Rıza Akgül | Ali Rahmi Fırat | Şükran Yalçın Özdilek
In this study, macroalgae, microalgae and Bryophyta and Magnoliophyta members were investigated in Karamenderes Stream. The sampling was carried out between the dates of 19-23 July 2012, 09-10 October 2012 and 16-18 May 2013 and the sampling was made from eight stations, which were designated on the river and composed of different habitats. Microalgae flora is given as planktonic, epilitic, epipelic and epiphytic. A total of 107 taxa were identified. A total of 83 taxa were determined from microalgae. Of these, 32 are planktonic, 45 are epilitic, 57 are epiphytic and 60 are epipelic algae flora. Also 9 macroalgae, 1 Bryophyta and 14 Magnoliophyta members were determined.
Show more [+] Less [-]Floristic Patterns and Qualities of Forage Species from Mountainous Rangeland in the Middle Black Sea Region of Turkey
2020
İbrahim Aydın | Betül Pak | Duygu Algan | Nuh Ocak
This study was carried out to investigate the floristic features (family, preference by grazers, growth form and response to grazing) and qualities of forage species collected from mountainous rangeland (Akdağ Mountain, Ladik) in the middle Black Sea region of Turkey. Forage samples were collected five times by 15-day intervals from the before-flowering stage to the after-flowering stage in 2015 and 2016. The total number of species was 105 species belonging to 73 genera and related to 26 families. Of the total species, 20 were from Poaceae (19.0%), 30 were from Poaceae (28.6%). Rests of the species belonged to other families (52.4%) dominated by families such as Asteraceae (13/55) and Lamiaceae (6/55), of which 26 species were weeds harmful to animals. While the percentages of decreaser, increaser and invader species were 16.2, 12.4 and 71.4, those of annual, biennial and perennial species were 31.9, 2.9 and 65.2, respectively. The number of species preferred by grazing was 74 (70.5%), while the number of non-preferable species was 31 (29.5%). The ME (MJ kg-1), RFQ and quality category of legumes, grass, and other botanical families were found as 8.88±0.07, 130.9±3.05 and very good, 8.00±0.07, 83.2±1.62 and good, and 8.98±0.07, 141.0±3.62 and premium, respectively. These results can be used as a management tool to improve rangeland quality and sustainability. The evidence from this study is that floristic pattern is not merely a result of invader forage species, but also might be a beneficial result that deserves further study for mountainous rangelands.
Show more [+] Less [-]Consumers’ Preference and Perception of the Different Broiler Housing and Feeding Systems Among Staff and Students in a State University (Eskişehir Osmangazi University)
2020
Canan Kop Bozbay
In this study, an investigation of the preference and perception of the different broiler housing and feeding systems among staff and students of Eskisehir Osmangazi University, Turkey was undertaken. A structured questionnaire was administered with a face-to-face to 790 randomly selected respondents [257 academic staff (academicians), 166 non-academic staff (worker with a secondary school national graduate diploma) and 367 undergraduate students (students)]. Most respondents preferred chicken (63.3%) and beef (32.0%) meats to fish meat (4.7%). The number of academicians preferred broiler meat (16.8%) was lower than those of students (52.6%) and workers (30.6%). There were significant differences among respondents in terms of preference and perception of different production sources of chicken meat. The proportion of academicians who preferred conventional broiler meat (68.2%) was higher than students (25.4%) and workers (6.4%). The impact of mediatic information (disinformation) about broiler meat relating to ingredients and/or feed additives used in broiler nutrition was higher on academics and students compared to workers. The results of the study indicated that as the level of education increases, the negative perception increased due to false media reports about chicken meat production and subsequently, the preference for chicken meat decreased.
Show more [+] Less [-]Presence of Aflatoxin M1 in Cube Cheeses Produced in Sivas Region
2020
Sema Ağaoğlu | Süleyman Alemdar | Nazlı Ercan
In this study, the presence and residue levels of aflatoxin M1 (AFM1) were investigated in traditionally produced cube cheeses in Sivas and its region. For this purpose, 90 pieces of cube cheese were used as a material. Cheese samples were collected periodically in November and December 2016 from central sales location and surrounding villages. ELISA technique was applied in aflatoxin analyses. According to the analysis results, AFM1 was detected in total of the cube cheeses. The lowest level of AFM1 was found to be 2.16 ng/kg, the highest was 53.94 ng/kg and the mean was 6.36±0.87 ng/kg. AFM1 level vas determined one cheese sample above to the limit set by European Union.
Show more [+] Less [-]Effects of Adding Vermicompost to the Tort-Perlite Mixture on The Development of Pepper Seedlings
2020
Sezer Şahin | Naif Geboloğlu | Hakan Kartal
The aim of this study was to determine the effects of vermicompost application on the growth of pepper seedlings. Fertilizer doses and application time applied to plants are among the most important factors affecting seedling quality in seedling production. Vermicompost products have many advantages from plant development to soil regulating effects, antioxidant effect and the use of all kinds of wastes (plant, animal, industrial, etc.) in production. Seedling mortar (70% peat and 30% perlite), which was constituted in the study, constituted the control group of the study. Pepper seedlings were grown by adding different doses of vermicompost (5, 10, 20%) to this control group. The properties of the vermicompost used were pH: 7.5, EC: 2.15 dS m-1, C / N: 18.71, organic matter: 64%, N: 1.25%, K2O: 1.02%, P2O5: 0.55 %). In the study, full fertilizer (EC: 1) dose and semi fertilizer (EC: 2) dose were applied in fertilization. Köylüm pepper varieties was used in the study. Total output rate (%), stem length (cm), stem diameter (mm), number of leaves (number), stem length (cm), wet and dry leaf weight (g), wet and dry body weight (g), wet and dry root weight (g) properties were determined. According to the control group, 10 and 20% vermicompost application of pepper seedlings wet weight, dry body weights and wet and dry root dry weights were statistically increased. The first emergence of plant seedlings and their marketable times were withdrawn early by vermicompost application. At the end of the study, it was determined that some of the properties examined with vermicompost application to control mortar increased. Vermicompost application showed good performance in working as an organic fertilizer that can be mixed with peat and perlite mixture.
Show more [+] Less [-]Planning Conditions of Milking Parlours Under a Separate Roof from The Milking Cow Barns: A Simple Milking Parlour Project
2020
Ünal Şirin | Sedat Karaman | Fatih Mehmet Kızıloğlu
Milking parlours are concrete constructions where milk is milked from cows, stored and transferred. These create the last stage of the work flow chart in dairy cattle farms. Quality of milk is important in the production of milk and dairy products owing to the fact that milk is one of the basic food requirements of people. The quality of milk depends on the genetic conditions of the cows as well as their well-being, the appropriate level of care and environmental conditions and milk being obtained in a clean environment which isolated from negative external factors. Milking parlours should be planned under a separate roof from the dairy cattle barns in order to ensure hygiene conditions, facilitate the work flow and reduce the negative effects on the health of cows and people. However, not including very large enterprises, it is planned as an add-on to other items under the same roof with the dairy cattle barn due to reasons such as decreasing the cost in the small, medium and majority of large-scale enterprises and the society does not care about hygiene conditions. Production in this wise is not appropriate to ensure the requirements about hygiene conditions in the production of milk and dairy products, since the milking parlours are directly under the environmental conditions of the barn. In this paper, it is explained the criteria to be considered in the process to plan and design milking parlour and it is aimed the generalization of milking parlour be built as a construction separate from the dairy barn, with an exemplary milking parlour project with 2×12 fishbone milking system.
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