Powdery mildew affecting European hazelnut Corylus avellana L. trees in Turkey is caused by the obligate biotrophic fungus Erysiphe corylacearum. This fungal disease causes significant economic losses by reducing the yield and quality of hazelnuts. Loss-of-function mutations in the mildew resistance locus o (MLO) gene family of many plants confer high levels of broad-spectrum resistance to powdery mildew. The proteins encoded by the genes at the MLO locus are divided into approximately seven different conserved clades. Among them, the phylogenetic clade V has been shown to be involved in PM susceptibility, as inactivation of these genes leads to long-term disease resistance in dicotyledons. In this study, we examined the temporal expression pattern of three hazelnut MLO genes, previously identified as clade V, in response to powdery mildew infection in C. avellana cv. Tombul. Leaves are the main tissue affected by the powdery mildew pathogen in hazelnut plants. Analysis of MLO expression in hazelnut leaves showed that CavMLO2 and CavMLO6 were significantly upregulated after challenge with E. corylacearum, providing preliminary evidence that they may be involved in PM susceptibility. Thus, these results present a basis for the isolation and use of relevant genes in plant breeding for disease resistance. Moreover, expression profiles of the clade V MLO genes are also important to identify candidate genes that need to be silenced or edited for future molecular studies to obtain resistant hazelnut varieties.

Sugar beet is an important agricultural and industrial crop that contributes to Turkey’s economy. It is not only the source of sugar production in our country but also become an important sector with seed production. The seeds are one of the most important issues directly affecting the root yield and quality of sugar beet. There are a lot of registered sugar beet varieties and sufficient seed production in Turkey. According to the data in 2020 year, the seeds of sugar beet varieties were produced with a sowing area of 11.891 decare, seed production was 2.317 tons and 198 kg/da in seed yield. The seed amount is completely belonging to the seed companies operating in the private sector. Besides, 413 tons of sugar beet seeds were imported and 11.312 million dollars was paid, while 143 tons of seeds were exported and 1.584 million dollars income was obtained in 2020. The sugar beet plant produces leaves above the ground and root in the first year, and grows the stem, branch, flower and seeds in the second year. Therefore, seed production needs a long time and intensive labor requirement. In order to shorten this two-year period in seed production of sugar beet, firstly seedlings belonging to male and female lines of a hybrid variety are grown and then, the seed production is performed by transplanting these seedlings. In this review, commercial hybrid seed production of sugar beet is explanied in detail.

As a part of prospecting bioactive molecules from natural resources, the phytochemical composition and antioxidant properties of extracts of leaves and berries oilcake of Pistacia lentiscus L., collected from two sites (Mechat and Bordj-Ali) in the northeast region of Algeria, were investigated. Dried leaves and berries oilcake obtained after removing the oily part by mean of Soxhlet apparatus were macerated in methanol in order to recover their respective active extracts. The phytochemical content analysis showed that lentisk leaves of both sites were relatively rich in polyphenols, flavonoids, flavonols and proanthocyanidins. The phytochemical content of berries oilcake extract was lower than that of leaves, yet, samples of Bordj-Ali displayed higher values for all assayed phytochemicals compared to those of Mechat. The P. lentiscus leaves extract exhibited a strong radical-scavenging activity (IC50 = 10.46 µg/mL) against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals and a substantial inhibitory activity against H2O2 free radicals (20.23 - 25.92%). Furthermore, these extracts displayed a very strong reducing power (EC50 = 28.08 µg/mL) and total antioxidant capacity (104.07 - 159.39 mg EAA/g DW). The observed results correlated positively with total phytochemical content strongly plead in favor of valorization of this wild plant as a potential natural source of active biomolecules for food, cosmetics, and medicinal industry sectors.

Bu çalışmanın amacı atık bir ürün olan elekaltı fasulyenin yumurtlayan bıldırcın rasyonlarında ham ve kavrulmuş olarak kullanılmasının performansa, yumurta kalitesine ve serum metabolik profiline etkisini incelemektir. Bu amaçla 70 günlük yaşta toplam 160 adet dişi Japon bıldırcını 4 seviye (0, 6, 12 ve 18) elekaltı fasulyenin ve 2 işlem tekniğinin (ham ve kavurulmuş) oluşturduğu 8 muamele grubuna 5 tekerrürlü olarak rastgele dağıtılmıştır. Kontrol grubu ile karşılaştırıldığında yem tüketimi ve yemden yararlanma oranı %12 elekaltı fasulye seviyesinde olumsuz etkilenmiş ve %18 seviyesinde olumsuz etki daha da belirgin olmuştur. Yumurta kitlesi ve kabuk kırılma direnci rasyonda elekaltı fasulye kullanımından istatistiki olarak etkilenmiş ve bu parametreler için en iyi sonuçlar %6 seviyesinde elde edilmiştir. Kabuk kalınlığı %12 ve 18 elekaltı fasulye seviyesinde önemli derecede azalmıştır. Bıldırcınların serum glukoz konsantrasyonu rasyonda %18 elekaltı fasulye kullanımı ile ve serum fosfor konsantrasyonu ise %6 seviyesinde elekaltı fasulye kullanımı ile önemli derecede artmıştır. Elekaltı fasulyenin kavrulması ile yumurta verimi, yumurta kitlesi ve yem değerlendirme ile serumun total protein, albümin ve fosfor konsantrasyonları önemli derecede iyileşmiştir. Elekaltı fasulye seviyesi ve işlem tekniğinin oluşturduğu interaksiyonları sadece kabuk kalınlığına ve serum glukoz konsantrasyonunu istatistiki olarak etkilemiştir. Çalışmadan elde edilen sonuçlara göre elekaltı fasulye yumurtlayan bıldırcın rasyonlarında %6 seviyesine kadar kullanılabileceği ve kavurma işleminin performans ve serum parametrelerini iyileştirdiği gözlenmiştir.

Leonardite and compost are used as soil conditioner materials. Gyttja is a material which contains 30-40% lime and 40-50% organic matter with a reserve of approximately 4.8 billion tons in Afşin-Elbistan Thermal Power Plant basin. These materials are used in the production of organomineral (OMG) fertilizers. Zinc (Zn) is one of the micronutrients that plants need. Available Zn deficiency is observed in approximately 49% of Turkish soils. The aim of this study was to determine the Zn adsorption properties of gyttja, leonardite and compost used in OMG fertilizer production. The study was conducted under laboratory conditions. In order to determine the optimum adsorption conditions, the effect of pH, particle size, temperature, shaking time and solution concentration were determined. Then, adsorption properties were determined using linear Langmuir isotherm under optimum conditions. According to Langmuir isotherm, the maximum sorption capacity (qmax) was found to be 14.10 mgg-1 for gyttja, 13.74 mgg-1 for leonardite and 15.67 mgg-1 for compost. In conclusion, Zn sorption values of gyttja, leonardite and compost were consistent with Langmuir and Freunlich isotherms. The maximum sorption capacity (qmax) of these materials is very close to each other. When these data are taken into consideration, it is seen that 1 kg of organic material can sorbed about 15 g Zn. It is useful to take these basic data into account in OMG fertilizer production and sustainable Zn fertilization.

It has been determined in many research results that plant growth promoting rhizobacteria (PGPR) affect yield, plant growth and fruit quality and play an important role. However, the use of biostimulants in agricultural production in Malatya is negligible. In order to contribute to the region's producers, a research was planned directly in the producer's garden in the Malatya/Arguvan region, which is an important melon production center. A trial was established with Arguvan (Narmikan) melon type and Kırkağaç 637 melon cultivars, which are the most grown local cultivars in the summer period of plant growth-promoting rhizobacteria in irrigated and non-irrigated melon cultivation in Malatya-Arguvan conditions, and the trial was repeated for two consecutive years. In the research, Bacillus subtilis, Bacillus megatorium, Enterococcus spp. and cocktail bacteria solutions, which are a mixture of these three bacteria were used. Inoculation of bacteria into seeds was done by soaking the seeds in bacterial solution for 24 hours. The effects of the use of bacteria on the fruit quality of melon in cultivation with the method of seed sowing in the field were determined. In the study, the presented of which are resulted in fruit only pomological porperties ; fruit height, fruit weight, fruit flesh thickness, fruit shell thickness, fruit diameter, seed cavity diameter, pH and WTSS contents were examined, and two-year results were presented. The effects of different bacteria on the fruit pomological properties were not found statistically significant except for the TSS values of Kırkağaç 637 cultivars grown under non-irrigated conditions. The effects of different bacteria on the fruit pomological characteristics of Kırkağaç 637 melons grown under non-irrigated conditions were found statistically significant, although there were differences in other parameters, it was not statistically significant. Bacillus subtilis bacteria application has been the prominent bacterial application in terms of fruit characteristics.

In this study, dog traffic in the animal shelter of Sivas province was investigated both quantitatively on the basis of years and the reasons for abandonment, as well as the attitudes and behaviors of the animal owners. In the study, besides the dog traffic records of the stray animal rehabilitation center operated by Sivas Municipality, the survey data made with the owners who left their dogs there were analyzed. According to the four-year records (2019-2022), the average number of dogs staying in the Sivas Municipality Animal Shelter is 1058, while the average number of dogs collected from the street per month is 50.7, the average number of spayed female and male dogs per month is 17.33 and 33.44, respectively. The average number of adopted female and male dogs was 2.8 and 4.3 respectively and, dog deaths per month in the shelter was 21.9. Every month, an average of 15 dogs were released back to the point where they were taken after the spayed and treated at the animal shelter. In addition, a face-to-face survey was conducted with a total of 50 people who left their dogs in the shelter and 15 questions were asked to the participants, including the reasons for adopting/abandonment and form of care-feeding. As a result, it can be said that it would be beneficial to deal with the process in many different provinces with more detailed studies in terms of the ever-increasing number of dog acquisitions in the society and the prevention of their abandonment.

Urban roads play a significant role for analyzing the complex structure of the city. The climatic features of the city provide information about the comfort of the inhabitants. The climate changes may be observed on a micro-scale due to the increase of impermeable surfaces. Surfaces such as concrete and asphalt contribute to the formation of an urban heat island as they store the temperature. This study aims to determine the effects of plant material, which is a significant element of urban roads, on temperature arising from surface materials. In this context, the effect of Ficus retusa-nitida on the formation of temperature caused by the surface material has been revealed. The study was carried out in Iskenderun, the second largest district of Hatay province in terms of population, in 3 stages in a periold of 6-months between July and December 2020. In the first stage, the studies on the subject were brought together and the points to be measured in the area were determined. At this stage, asphalt (shade-under F.retusa-nitida, sun) and parquet (shade-under F.retusa-nitida, sun) surfaces were selected at a total of 8 points located mutually on the main road axis. The second stage of the study is the stage where surface temperature measurements are made. Measurements were performed once a month at 06:00, 09:00; 12:00, 15:00; 18:00; 21:00 and 24:00 using an infrared thermometer at a height of 150 cm from the surface with 3 repetitions. At the third stage, all data were transferred to GIS using ArcGIS 10.5 software and modelled by using the Kriging Interpolation Method. In line with the results obtained, suggestions for the selection of surface material and the use of plant materials on urban roads have been developed.

The obtention of homozygous lines through in situ parthenogenesis via gamma irradiation method is a comprehensive application in vegetables. However, there are a limited number of studies on in situ parthenogenesis in ornamentals. Therefore, in situ parthenogenetic capacity of more species needs to be examined. For this purpose, the effects of pollination with gamma-irradiated pollen on in vitro ovule cultured in Cyclamen persicum L. were evaluated in this study. Flower buds were collected before anthesis and irradiated at different doses 50, 100, 150, 200, 300, and 450 Gy of gamma-ray using a Co-60 source. The control group was pollinated with non-irradiated pollen. Fruits were harvested 30 days after pollination, and isolated ovule explants were cultured on four different mediums in in vitro. M0 was control group containing half-strength MS basal media; M1 media additionally has 10 g/L maltose, 1.0 g/L proline, 2.0 g/L peptone, 200.0 mg/L spermidine and 0.5 mg/L kinetin; in addition to basal medium, M2 media additionally contains 10 g/L maltose, 1.0 g/L proline, 2.0 g/L peptone, 200.0 mg/L spermidine, 0.4 mg/L gibberellic acid (GA3) and 0.4 mg/L N6-benzyl adenine (BA). M3 media additionally contains 2.0 mg/L 2,4-D and 0.8 mg/L 6-( γ, γ-dimethylallylamino) purine (2iP). Plantlets started to form 10-12 weeks after the beginning of culture. The effects of nutrient media, irradiation dose, and their interactions on plant formation were statistically significant. The lowest plantlet regeneration (0.33%) was obtained from ovule explants exposed to 50 Gy gamma rays and cultured on M3 media while the highest plantlet regeneration (2.66%) was obtained from ovule explants cultured on M1 media 30 days after pollination with non-irradiated pollen grains. According to stomatal observations, there were no statistical differences between donor plant and in vitro regenerated plantlets.

This study aimed to investigate the presence of Aflatoxin M1 (AFM1) in raw buffalo milk and buffalo milk products such as cheese, yogurt, cream, and ice cream by ELISA technique. In the study, 175 samples were investigated, and it was determined that 146 (83.43%) of the samples had AFM1 concentrations below the LOD (limit of detection) value. In 29 samples (16.57%), the AFM1 concentration exceeded the LOD value, and in 7 (4%) of these, the AFM1 concentration was above the legal limits (50 ng/L). While AFM1 was not detected in raw buffalo milk samples, the presence of AFM1 in cheese, yogurt, cream, and ice cream samples was found to be 2.85%, 2.85%, 62.85%, and 14.3%, respectively. 2.85% of cheese samples and 17.1% of cream samples were found contaminated with AFM1 above the allowed legal limits (50 ng/L). As a result, it was determined that the potential for exposure to AFM1 by consuming buffalo cream was higher than the consumption of buffalo milk and other buffalo milk products. However, the detection of AFM1 above legal limits in buffalo milk products poses a serious risk to public health. Therefore, it is extremely necessary for public health to develop effective monitoring programs to control aflatoxin contamination in milk and new strategies to maintain inspections.