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Bazı Anadolu Yerli Sığır Irklarının MtDNA D-loop Dizi Analizi: Genetik Çeşitlilik ve Popülasyon Geçmişinin Değerlendirilmesi Full text
2023
Müge Doğan | Mehmet Nizamlıoğlu
Bazı Anadolu Yerli Sığır Irklarının MtDNA D-loop Dizi Analizi: Genetik Çeşitlilik ve Popülasyon Geçmişinin Değerlendirilmesi Full text
2023
Müge Doğan | Mehmet Nizamlıoğlu
Maternal kalıtımın moleküler göstergesi olan mitokondrial DNA, genomik DNA‘ya oranla daha hızlı evrimleşmesi, rekombinasyonun olmayışı ve türlerin coğrafi dağılımına göre farklılıklar göstermesi gibi özelliklerinden dolayı popülasyon genomiği, filogenetik ve filocoğrafik çalışmalarda sıklıkla tercih edilen belirteçlerden birisidir. Bu çalışmada Anadolu'da yetiştirilen bazı yerli sığır ırklarının, ırk içi ve ırklar arası genetik çeşitlilikleri ile filogenetik ilişkilerinin ortaya konulması amacıyla mitokondrial DNA D-Loop bölgesi dizi analizleri gerçekleştirildi. Öncelikle araştırmaya konu olan sığır ırklarının örnekleme çalışması tamamlandı. Standart Fenol/Kloroform Yöntemi kullanılarak DNA izolasyonu yapılan örneklerin mitokondrial DNA D-Loop bölgesinin yükseltgenmesi ise Polimeraz Zincir Reaksiyonu ile yapıldı. DNA dizi analizi sonrası yerli sığır ırklarına ait veriler GenBANK’ tan alınan referans mitokondrial DNA dizileri ile birlikte hizalandı. Haplotip ve nükleotid çeşitlilikleri ile popülasyon içi ve popülasyonlar arası genetik ve filogenetik ilişkileri değerlendirildi. Elde edilen veriler ile Anadolu yerli sığır ırklarının, nükleotid ve haplotip çeşitliliği, haplotip sayıları ile popülasyon içi ve popülasyonlar arası varyasyonun oldukça yüksek olduğu belirlendi. Anadolu da yetiştirilen yerli sığır ırklarının filogenetik olarak Asya ve Avrupa sığır ırkları arasında yer aldığı sonucuna varıldı.
Show more [+] Less [-]The battle against rural poverty and other challenges of development: Empirical analysis of women empowerment programme of Justice, Development and Peace Movement (JDPM) in Osun State, Nigeria | La batalla en contra de la pobreza rural y otros retos de desarrollo: Análisis empírico del programa de justicia y empoderamiento femenino, movimiento de paz y desarrollo (JDPM) en el estado de Osun, Nigeria Full text
2016
Faborode, Helen | Alao, Titus Olugbenga
The vital role played by women in agriculture and non-farm activities for achieving food security and economic growth led to the recognition of women as a vital instrument by both government and non-governmental organizations in the ‘battle’ against rural poverty and other challenges of development process. Hence, this article analysed the women empowerment programme of Justice, Development and Peace Movement (JDPM) of Osogbo Catholic Diocese in Osun State with a view to enhancing its effectiveness. The impact of the programme on the beneficiaries was assessed on four randomly selected communities from each of the three administrative zones of Osogbo, Ila and Ilesa. Twenty-five percent of the participants were proportionately sampled from each community selected, making 104 respondents. Structured interview schedule was performed for each data collection from programme beneficiaries while seven key informants were interviewed among the workers of the Diocese. Descriptive statistics (frequency counts, mean, percentages and standard deviation) and Pearson correlation was used to make inferences. Some of the results revealed that members of all religions practiced in the area benefitted from the programme. Results also revealed that age (r= -0.514), group size (r= -0.448), years of schooling (r=0.407) were significantly related to achievement of programme objectives at 0.01 level of significance while number of community associations (r=0.201), size of enterprise (r=0.448), and income (r=0.205) had significant relationship at 0.05 level of significance. The study also revealed that the programme had made the beneficiaries self reliant through skill acquisition but faced with financial constraint to start or expand the businesses learnt. It was recommended that the beneficiaries should be linked with financial institutions or banks where they could access loan with ease. | El rol vital ejercido por la mujer en la agricultura y en actividades no agrícolas para el establecimiento de la seguridad alimentaria y el crecimiento económico, ha llevado al reconocimiento de la mujer como un instrumento vital tanto para el gobierno como para organizaciones no gubernamentales en la batalla en contra de la pobreza rural y otros retos de procesos de desarrollo. Por lo tanto, esta investigación analizó el programa de justicia y empoderamiento femenino, el movimiento de paz y desarrollo (JDPM) de la diócesis católica de Osogbo en el Estado de Osun, Nigeria con el fin de mejorar su eficacia. El impacto del programa sobre los beneficiarios fue evaluado en cuatro comunidades seleccionadas aleatoriamente de cada una de las tres zonas administrativas de Osogbo, Ila e Ilesa. El 25% de los participantes fueron proporcionalmente muestreados de cada una de las comunidades seleccionadas obteniendo 104 encuestados. La entrevista estructurada fue presentada para cada colecta de datos a partir del programa de beneficiarios, mientras que siete informantes clave eran entrevistados entre los trabajadores de la diócesis. La estadística descriptiva (frecuencia, promedio, porcentajes y desviación estándar), así como la correlación Pearson fueron utilizados para realizar inferencias. Algunos de estos resultados revelaron que los miembros de todas las regiones practicaban en el área beneficiada del programa. Los resultados también revelaron que la edad (r= -0.514), el tamaño del grupo (r= -0.448), los años de escolaridad (r=0.407) estaban significativamente relacionados con el establecimiento de los objetivos del programa con un nivel de significancia del 0.0, mientras que el número de asociaciones comunitarias (r=0.201), el tamaño de la empresa rural (r=0.448), y los ingresos (r=0.205) presentaron una relación significativa con un nivel del 0.05 de significancia. El estudio también reveló que el programa ha hecho independientes a los beneficiarios gracias a la adquisición de herramientas pero también al enfrentarse con restricciones financieras para iniciar o expandir su negocio. Se recomendó que los beneficiarios deberían estar vinculados con instituciones financieras o bancos donde podían acceder a préstamos con facilidad.
Show more [+] Less [-]The battle against rural poverty and other challenges of development: Empirical analysis of women empowerment programme of Justice, Development and Peace Movement (JDPM) in Osun State, Nigeria Full text
2016
Faborode, Helen(Obafemi Awolowo University Department of Agricultural Extension and Rural Development) | Olugbenga Alao, Titus(Osun State University Department of Agricultural Economics and Extension College of Agriculture)
The vital role played by women in agriculture and non-farm activities for achieving food security and economic growth led to the recognition of women as a vital instrument by both government and non-governmental organizations in the ‘battle’ against rural poverty and other challenges of development process. Hence, this article analysed the women empowerment programme of Justice, Development and Peace Movement (JDPM) of Osogbo Catholic Diocese in Osun State with a view to enhancing its effectiveness. The impact of the programme on the beneficiaries was assessed on four randomly selected communities from each of the three administrative zones of Osogbo, Ila and Ilesa. Twenty-five percent of the participants were proportionately sampled from each community selected, making 104 respondents. Structured interview schedule was performed for each data collection from programme beneficiaries while seven key informants were interviewed among the workers of the Diocese. Descriptive statistics (frequency counts, mean, percentages and standard deviation) and Pearson correlation was used to make inferences. Some of the results revealed that members of all religions practiced in the area benefitted from the programme. Results also revealed that age (r= -0.514), group size (r= -0.448), years of schooling (r=0.407) were significantly related to achievement of programme objectives at 0.01 level of significance while number of community associations (r=0.201), size of enterprise (r=0.448), and income (r=0.205) had significant relationship at 0.05 level of significance. The study also revealed that the programme had made the beneficiaries self reliant through skill acquisition but faced with financial constraint to start or expand the businesses learnt. It was recommended that the beneficiaries should be linked with financial institutions or banks where they could access loan with ease | El rol vital ejercido por la mujer en la agricultura y en actividades no agrícolas para el establecimiento de la seguridad alimentaria y el crecimiento económico, ha llevado al reconocimiento de la mujer como un instrumento vital tanto para el gobierno como para organizaciones no gubernamentales en la batalla en contra de la pobreza rural y otros retos de procesos de desarrollo. Por lo tanto, esta investigación analizó el programa de justicia y empoderamiento femenino, el movimiento de paz y desarrollo (JDPM) de la diócesis católica de Osogbo en el Estado de Osun, Nigeria con el fin de mejorar su eficacia. El impacto del programa sobre los beneficiarios fue evaluado en cuatro comunidades seleccionadas aleatoriamente de cada una de las tres zonas administrativas de Osogbo, Ila e Ilesa. El 25% de los participantes fueron proporcionalmente muestreados de cada una de las comunidades seleccionadas obteniendo 104 encuestados. La entrevista estructurada fue presentada para cada colecta de datos a partir del programa de beneficiarios, mientras que siete informantes clave eran entrevistados entre los trabajadores de la diócesis. La estadística descriptiva (frecuencia, promedio, porcentajes y desviación estándar), así como la correlación Pearson fueron utilizados para realizar inferencias. Algunos de estos resultados revelaron que los miembros de todas las regiones practicaban en el área beneficiada del programa. Los resultados también revelaron que la edad (r= -0.514), el tamaño del grupo (r= -0.448), los años de escolaridad (r=0.407) estaban significativamente relacionados con el establecimiento de los objetivos del programa con un nivel de significancia del 0.0, mientras que el número de asociaciones comunitarias (r=0.201), el tamaño de la empresa rural (r=0.448), y los ingresos (r=0.205) presentaron una relación significativa con un nivel del 0.05 de significancia. El estudio también reveló que el programa ha hecho independientes a los beneficiarios gracias a la adquisición de herramientas pero también al enfrentarse con restricciones financieras para iniciar o expandir su negocio. Se recomendó que los beneficiarios deberían estar vinculados con instituciones financieras o bancos donde podían acceder a préstamos con facilidad
Show more [+] Less [-]Probiyotik Bakterilerin Stress Koşullarının Azaltılmasında Alternatif Proses Teknolojileri Full text
2023
Deniz Aksöz | Tülay Özcan
Probiyotik mikroorganizmalar sıcaklık, pH, oksijen ve su aktivitesi gibi faktörler ile birlikte ürün matriksi ve gıda işlem proseslerinden büyük ölçüde etkilenmektedir. Isıl işleme alternatif teknolojiler, artan probiyotik canlılığı, prebiyotik stabilite ve daha yüksek biyoaktif bileşik konsantrasyonuna sahip yenilikçi gıda ürünlerini tasarlamak için son yıllarda giderek önem kazanmaktadır. Gelişen bu alternatif teknolojilerin probiyotik ürünlere uygulanması, bakteri canlılığını arttırmakla birlikte, probiyotik fermantasyonu ile kolesterolün bağlanması, Caco-2 hücrelerine yapışma, anjiyotensin dönüştürücü enzim (ACE) inhibitörünü artırma, antioksidan, antimikrobiyal aktivite ve sistolik kan basıncını düşürme gibi biyolojik etkileri iyileştirmektedir. Ayrıca, bu teknolojiler ile fermantasyon kinetikleri optimize edilmekte, bakteri faaliyetleri sonucunda bakteriosin, oligosakkarit, peptit, fenolik bileşik, flavonoidler gibi bileşikler üretilmekte, fermente edilmekte, korunmakta, biyoaktif (vitamin ve mineral biyoyararlılığı) ve duyusal özellikler iyileştirilmektedir. Son yıllarda artan sağlık etkilerine sahip postbiyotikler elde etmek için de gelişen bu teknolojiler uygulanmaktadır. Çalışmalar, alternatif işleme teknolojilerinin gıdalardaki probiyotik, prebiyotik ve postbiyotiklerin terapötik etkisini artırdığını göstermektedir. Bu derlemede, ısıl işleme alternatif teknolojilerin probiyotik fermantasyonu üzerindeki etkisi incelenmektedir.
Show more [+] Less [-]In Vitro Shoot Bioassay of Salt Tolerant International Potato Center Bred Potato Genotypes for Assessing Their Salinity Tolerance Full text
2023
Md. Habibur Rahman | Deen Mohammad Deepo | Md Mazahul Islam | Md. Abul Bashar | Kamrun Nahar Sheuly | Khalid Syfullah | Md. Ekramul Hoque | Md Moshrraf Hossain Molla
In Vitro Shoot Bioassay of Salt Tolerant International Potato Center Bred Potato Genotypes for Assessing Their Salinity Tolerance Full text
2023
Md. Habibur Rahman | Deen Mohammad Deepo | Md Mazahul Islam | Md. Abul Bashar | Kamrun Nahar Sheuly | Khalid Syfullah | Md. Ekramul Hoque | Md Moshrraf Hossain Molla
The aim of the experiment is to study in vitro regeneration efficiency of international potato center (CIP)-bred salt tolerant potato genotypes under salt stress condition and to identify effective potato genotype(s) for saline belt areas of Bangladesh. An in vitro shoot bioassay of eight CIP-bred potato genotypes viz. CIP 102, CIP 106, CIP 111, CIP 117, CIP 124, BARI Alu 72 (CIP 139), and BARI Alu 73 (CIP 127) and CIP 136 were used. In this study, single node of these genotypes was cultured in MS media supplemented with 0, 80, 100, 120, 140 and 160 mM NaCl. Among the eight genotypes, BARI Alu 72 (CIP 139) showed the highest tolerance against salinity up to 160 mM NaCl (14.61 dS/m) for all studied parameters (except shoot and root initiation) with the highest plant height (9.67 cm), leaves number (13.60), nodes number (9.50), root length (6.50 cm), roots number (7.80), fresh weight of shoot (536.1 mg) and root (205.60 mg). On the other hand, CIP 106 was found the most susceptible genotype against salinity showing its highest salinity tolerance up to 120 mM NaCl (10.96 dS/m) with maximum plant height (7.17 cm), leaves number (12.50), nodes number (6.50), root length (7.50 cm), roots number (9.7), fresh weight of shoot (572.3 mg) and root (250 mg). The experiment's findings corroborated CIP's findings that they were salt tolerant, as well as recommended for their cultivation suitability in saline-affected area in Bangladesh.
Show more [+] Less [-]Efecto de la solarización sobre Rhizoctonia spp. en semilleros de tomate, Lycopersicum esculentum, var. tropic Full text
1990
Ruíz, Leyder J. | Bravo Otero, Nelson
A research was carry out in Palmira (Valle, Colombia) to test the solarization (poliethylene mulch). The experimental design consisted in "random blocks" and ten treatments obtained from the following factors combination: soil solarization during 2, 4 and 6 weeks, chemical treatrnent (Dazornet), control, all of them with and without application of rabbit manure (2 kg). The plot size was 1.44 m2. The soil was inoculated with the mixture of four isolations of Rhizoctonia solani which were pathogenics to the tomato (Tropic Variety). Temperatures of 49.7oC and 44.8oC at 5 and 10 cm deep respectively were reached in the soil under solarization treatment. The control reached temperatures of 41.6 y 38.8oC at the same depths. Rhizoctonia population was reduced to 0.8 colony formation units (C F U) per 100 g of soil in the solarization treatment, during the first experiment (september to october 1987). In the second experiment (january to february 1988) the CFU was reduced to zero. The chemical treatment reduced the CFU to zero in both experiments. The control plots showed and average of 24.6 CFU during both experiments. The number of seedlings per row and the number of healthy plants (93% in both experiments) was greater in the soil under chemical and solarization treatment than the control treatment. This showed 52.3 % and 88.7% of healthy plants in both experiments. The plots where the manure was applied the number of plants per row decreased in 3.3 (solarization) and 15.3 % (chemical), during the first experiment. The second experiment showed that the number of plants per row increased in 19.2% and 9.5% to the same treatments. | En Palmira (Valle, Colombia) se comparó la solarización del suelo (cubrimiento con láminas de polietileno) durante 2, 4 y 6 semanas, con un producto químico (Dazomet) para la desinfestación de semilleros abonados con conejinaza (2 kg en 1.44 m2) y sin abonar, en presiembra. El suelo se inoculó con una mezcla de cuatro aislamientos de Rhizoctonia solani patogénicos a tomate variedad Tropic. En los suelos solarizados se alcanzaron temperaturas de 49.7 y 44.8 C a 5 y 10 cm de profundidad respectivamente y 41.6 y 38.8C en los testigos a las mismas profundidades. La solarización redujo la población de Rhizoctonia, en promedio, a 0.8 U.F.C (unidades formadoras de colonias) por 100 g de suelo, en el primer ensayo (septiembre-octubre de 1987) y a cero en el segundo ensayo (enero-febrero de 1988); el Dazomet la redujo a cero en los dos ensayos. El testigo presentó un promedio de 24.6 U.F.C para los dos ensayos. El número de plántulas por surco fue mayor en los suelos tratados (solarización y producto químico) lo mismo que el porcentaje de plántulas sanas (93 %), en los dos ensayos; el testigo presentó 52.3 y 88.7% plántulas sanas en los dos ensayos respectivamente. En los semilleros abonados el número de plántulas por surco fue menor en 3.3 (solarización) y 15.3 % (producto químico) para el primer ensayo, pero fue mayor, 19.2 y 9.5%, en el segundo ensayo.
Show more [+] Less [-]Characterization of Lactose-Free Dulce de Leche Full text
2023
Olcay Mercan | Zerrin Yüksel
The aim of the study is to be determine the effect of lactose hydrolysis and sugar content on physicochemical properties, sensory profile and HMF (5-hydroxymethylfurfural) content in Dulce de Leche (DL). Lactose free (lactose-hydrolysed) dairy products as well as low sugar products have been developed to supply consumer demand. Two different sucrose concentrations, 16% and 20%, were used in milk jam samples produced by the traditional method. For the formation of desired colour and flavour in the Dulce de Leche, Maillard reaction products are mainly responsible. HMF is Maillard reaction indicator analysed in this work. Fat, protein, solid content, ash, lactic acid and pH analysis were carried out. Sucrose, glucose, fructose and lactose concentrations were determined. Lightness, yellowness and redness as colour parameters of DL samples were evaluated and sensory analyses were also performed. It has been revealed that lactose hydrolysis causes significant changes in the color parameters and sensory profile of the samples. In this study, less or no HMF was detected in the lactose hydrolyzed DL samples compared to the control samples.
Show more [+] Less [-]Gıda Sistemlerinde Yenilebilir Filmler ve Kaplamalar Full text
2023
Semra Bozkurt | Özgül Altay | Mehmet Koç | Figen Kaymak Ertekin
Sentetik ambalajların çevre üzerindeki olumsuz etkilerinin azaltılması ve gıdaların doğal yollarla korunmasına yönelik tüketici taleplerinin artması bu alanda alternatif yöntemlerin araştırılmasına öncülük etmiştir. Ambalaj teknolojisindeki yeniliklerden biri olan yenilebilir film ve kaplamalar, nem, gaz, yağ ve aroma geçişine karşı bariyer işlevi görerek gıdaların raf ömrünü uzatmak ve kalitesini iyileştirmek amacıyla kullanılmaktadır. Gıdaların yüzeyine ince bir tabaka şeklinde uygulanan veya ambalaj materyali olarak kullanılabilen yenilenebilir film ve kaplamaların üretiminde protein, polisakkarit ve lipid gibi farklı biyopolimerler kullanılmaktadır. Bu biyopolimerlere ek olarak yenilebilir film ve kaplamaların mekanik ve fonksiyonel özelliklerini geliştirmek amacıyla plastikleştirici madde, emülsifiyerler, antioksidan ve antimikrobiyal maddelerden de yararlanılabilmektedir. Filmlerin yapılma tekniği, kullanılan kaplama materyali tipi, kullanılan plastikleştirici madde cinsi ve konsantrasyonu, film hazırlama aşamasında yapılan kurutma işlemi ve çevresel faktörler (sıcaklık, bağıl nem, basınç) yenilebilir film veya kaplamaların fiziksel ve mekanik özelliklerini etkileyen önemli faktörlerdir. Bu derleme makalede yenilebilir film ve kaplamalar için kullanılan film materyalleri, film hazırlama yöntemleri, filmlerin yapısını etkileyen çevresel ve yapısal faktörler bir arada derlenmiş, bu faktörlerin filmlerin kalite özellikleri üzerindeki etkileri değerlendirilmiştir. Aynı zamanda yenilebilir filmlerin gıdalarda kullanım alanları da değerlendirilmiştir.
Show more [+] Less [-]Use of Probiotics for Safe Quail Meat Production Full text
2023
Prodip Kumar Sarkar | Dip Majumder Ridoy | Mehedi Islam Moon | Swapon Kumar Fouzder
Safe meat production is an important aspect to avoid human health hazards. The use of probiotics in poultry is an important tool to produce safe meat among several established biotechnological approaches. In this experiment, we studied the effects of probiotics for producing safe Japanese quail meat. 150 Japanese quail chicks were reared for a period of six weeks using various doses of probiotics (0, 0.5, 1, 1.5, and 2g per litre of water). The chicks were randomly distributed into five treatment groups with three replications each. The number of birds in each replication was 10. After rearing six weeks, significantly high body weight was found at probiotic concentrations of 1, 1.5, and 2g per litre of water. The feed intake in various treatments did not differ significantly, but comparatively better feed conversion ratios were observed at probiotic treatments. Water quality was not significantly differed as a result of addition of probiotics to the water. The reason for this better growth performance is probably due to the multiple benefits of probiotics in poultry. Probiotics could have maintained gut health with better nutrient utilization and availability that might have been led to higher body weight gain in the quail. In future experiments, challenging the birds with diseases or comparing probiotics with antibiotic growth promoters is required to ensure the efficiency of probiotics.
Show more [+] Less [-]Effect of Different Organic and Inorganic Fertilizers on Spring Rice Var. (Hardinath 1) Production in Rural Gorkha, Nepal Full text
2023
Sandesh Adhikari | Sudip Tiwari | Binaya Baral | Sandeep Gouli | Shiva Chandra Dhakal | Manisha Shrestha
The effect of different organic and inorganic fertilizers had a significant effect on the yield of spring rice. A field experiment was carried out to determine the effects of different organic and inorganic fertilizers on the yield of spring rice var Hardinath-1 at Siranchowk rural municipality-4, Gorkha during the spring season of 2021 with four replications and five treatments: T1[Recommended inorganic fertilizer Nitrogen, Phosphorous, and Potassium (@100:30:30 kg/ha)], T2[75% recommended inorganic fertilizer + 25% recommended organic fertilizer: i.e. (Farm Yard Manure (6 ton /ha)], T3(50% recommended inorganic fertilizer + 50% recommended organic fertilizer), T4(25% recommended inorganic fertilizer + 75% recommended organic fertilizer ), and T5 (Recommended organic fertilizer) using simple Randomized Complete Block Design with 3*3 m of individual plot size. A biometrical observation like plant height (95.33 cm), and the number of tillers (340.75/ m2) were found significantly highest on T2. Similarly, yield attributing characteristics like the number of effective tillers (315.75/m2), several filled grains per panicle (150.25), and highest panicle length (25.06 cm) were also significantly highest on T2. The highest grain yield (8.27 ton/ha), straw yield (12.14 ton/ha), and harvest index (0.40) were also observed on T2. Total net return was found highest on T2: 287330 Nepalese Rupees(NPR) incurring the cost of fertilizer Rs NPR 18940 per hectare returning the gross return of NPR 268390 per hectare. In conclusion, T2 was the best combination of other biofertilizer combinations. So, the spring rice productivity can be enhanced via the adoption of 75% recommended inorganic fertilizer + 25% recommended organic fertilizer in fertilizer management practice at Chorkate, Gorkha.
Show more [+] Less [-]Exploring the Efficacy of Essential Oils in Laboratory Conditions for Controlling Mediterranean Fruit Fly Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) Full text
2023
Esengül Özdemir
The Mediterranean fruit fly (Medfly), Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), poses a significant threat to agriculture worldwide. This study examines the potential insecticidal effects of essential oils from Mentha arvensis and Cinnamomum zeylanicum on controlling C. capitata under laboratory conditions. Even at low concentrations, toxicity assays indicated that both essential oils significantly increased the mortality of adult Medflies. The concentration-dependent effect of these oils on C. capitata mortality is demonstrated, with Mentha arvensis achieving 100% mortality within 48 hours at 1% concentration and Cinnamomum zeylanicum exhibiting rapid efficacy, reaching a low LC50 value after only 1 hour of application. The concentration and application time of essential oils were found to have a significant impact on their effectivness. This study highlights the potential of essential oils for controlling C. capitata populations. Essential oils offer a sustainable and eco-friendly alternative for managing C. capitata but further studies are necessary for their successful incorporation into integrated pest management programs.
Show more [+] Less [-]The Effects of Seed Infestation by Fusarium proliferatum on Root and Crown Rot, Plant Growth and Phenolic Compounds in Roots of Some Pumpkin (Cucurbita pepo L.) Cultivars Full text
2023
Ebru Sevinç | Nuray Özer
This study investigates the reactions of four summer pumpkin cultivars (cvs. Çağlayan, Mert Bey, Sena Hanım, TG38) to root and crown rot caused by Fusarium proliferatum by taking into account criteria such as disease severity, plant growth (number of leaves, height, dry and fresh weight of shoot) and the accumulation of phenolic compounds in the roots. Seeds of each cultivar were inoculated with the pathogen and left to develop for 1 month at 25oC in a controlled climate room. The content of phenolic compounds in ethanolic root extracts was determined using high-performance liquid chromatography (HPLC). Cv. Sena Hanım had the lowest disease severity (4.40%) among the cultivars, followed by cvs. Çağlayan (10.62%) and Mert Bey (11.07%). Plants developed from inoculated seeds of cvs. Çağlayan and Sena Hanım had no decrease in the number of leaves and in length, fresh and dry weight of shoots in comparison to the control (plants from non-inoculated seeds), while cv. Mert Bey demonstrated a decrease at very low rates in shoot fresh and dry weight (2.24% and 0.77%, respectively). The phenolic compound that exhibited the highest increase in root extracts of cv. Sena Hanım compared to the control among the cultivars was p-coumaric acid (6.57-fold). This study demonstrates that p-coumaric acid can play an important role in the resistance of pumpkin to seed infestation by F. proliferatum.
Show more [+] Less [-]Determination of Probiotic Viability in Yoghurts Produced with Acid Adapted Lactobacillus acidophilus ATCC 4356 and Bifidobacterium bifidum ATCC 11863 During Refrigerated Storage Full text
2023
Şehriban Oğuz | Seval Andiç | Neşe Badak | Tekin Demir
Microorganisms have various stress response systems to maintain their viability when exposed to different stress conditions. In this study, Lactobacillus acidophilus ATCC 4356 and Bifidobacterium bifidum ATCC 11863 strains, used in probiotic yoghurt production, were subjected to acid (lactic and hydrochloric acid) stress to induce acid tolerance response (ATR). Yoghurts produced with both acid-adapted and non-adapted strains were stored at +4°C for 21 days. During the storage period, the pH and titratable acidity values of the yoghurts were measured, and the viability levels of the probiotic strains in the yoghurts were determined. In all yoghurt groups, a decrease in pH values and an increase in titratable acidity were observed during storage. The highest viability levels of the probiotic strains were detected on the first day of storage. Lactic acid-adapted Lb. acidophilus ATCC 4356 and B. bifidum ATCC 11863 in yoghurt showed growth at a level of 8.08 ± 0.12 and 8.08 ± 0.09 log10 Cfu/g at the first day of storage, respectively. Additionally, hydrochloric acid-adapted Lb. acidophilus ATCC 4356 and B. bifidum ATCC 11863 in yoghurt exhibited growth at levels of 7.90 ± 0.08 and 5.99 ± 0.03 log10 Cfu/g, respectively. The viability of acid-adapted Lb. acidophilus ATCC 4356 and B. bifidum ATCC 11863 showed a decrease similar way to that of the control group (non-acid adapted) during the storage period.
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