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Toxic Effect of Four Different Plant Extracts on Potato Tuber Moth [(Phthorimaea operculella(Zeller) (Lepidoptera: Gelechiidae)] Eggs and Larvae Full text
2019
Ayşe Yeşilayer | Melike Deniz
Two different thyme species (Thymus vulgaris L. and Origanum majorana L.), sage (Salvia officinalis L.) and lavender (Lavandula officinalis L.) extracts were tested against pre-adult stage of Potato tuber moth-PTM [(Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae)], which is an important quarantine pest. Each plant extracts prepared with ethanol were applied to larvae and eggs in three different concentrations 3%, 5 and 10 ml/L. In the egg treatments at the highest concentration (10% ml/L), inhibition rate was observed in Lavander and T. vulgaris 73.7%, in O. majorana 67.5% and in sage 66.2%, respectively. Results indicated that the mean number of hatching eggs were highly decreased as concentration increased, the highest decreased was observed with treated 10% concentration. of lavender and T. vulgaris. Observation were for 7 days. In the insecticidal toxicity study of against the larval stage, mortality were determined at the highest concentration (10% ml/L) in O. majorana 91.2%, Lavander 90%, T. vulgaris 87%, and Sage 83.7% at the end of 7th day. It was also determined that the larval mortality rate was higher with the increasing of extracts of 4 different plant depending on time. As a result, in this study, ovicidal toxicity of plant extracts was recorded against to on egg stage and it was that impact of insecticidal against to on larval stage also.
Show more [+] Less [-]Changes within the Daytime of Essential Oil Content and Composition of Zahter (Thymbra spicata L.) Grown in Ermenek Conditions Full text
2019
Hasan Maral | Saliha Kırıcı
Thymbra spicata var. spicata is known as “Zahter ”, “Sater” or “Karabaş thyme in the Southeastern Anatolia Region of Turkey and leaves and flowers are consumed as spices and tea, while young shoots are consumed as salads.This study was carried out to determine the change in essential oil content and chemical compositionduring the daytime of the Thymbra spicata plant in Ermenek district of Karaman.In order to determine the diurnal variation, the harvest was done at 5 different hours during the daytime (6:00, 9:00, 12:00, 15:00 and 18:00) in the flowering period. The harvested plants were dried in the shade and the essential oil contents were determined by Clevenger apparatus by hydro distillation and analyzed by GC-MS. The highest content of essential oil was obtained at 18.00 hours with 2.96% and the lowest one’s was obtained at 12.00 hours with 2.20%.The main components of the essential oil were determined as carvacrol (23.29% - 39.89%), thymol (16.24% - 21.52%) and γ-terpinene (18.01% - 20.90%).
Show more [+] Less [-]Weed Flora of Cherry, Walnut, Apple, Almond and Pear Orchards in Northwestern Marmara Region of Turkey Full text
2019
Bahadır Şin | Lerzan Öztürk | Nur Sivri | Gürkan Güvenç Avcı | İzzet Kadıoğlu
A study covering cherry, walnut, apple, almond and pear orchards in Edirne, Kırklareli and Tekirdağ provinces was carried out in 2015-2018 with the aim of determination of weed flora, weed frequencies and densities. Total of 64 species belonging to 30 families were identified in fruit tree orchards. Among all the families the most members were in Asteraceae 13 species, Poaceae, 11 species, Brassicaceae 5 species and Lamiaceae 3 species families. The dominant weed species were Amaranthus retroflexus L., Polygonum aviculare L., Capsella-bursa pastoris L. (Medik)., Concolvulus arvensis L., Lepidium draba L., Raphanus raphanistrum L., Chenopodium album L., Cichorium intybus L. in Tekirdağ while Datura stramonium L. distribution in Edirne Uzunköprü and Kırklareli was remarkably high. Semi parazitic weed Viscum album and full parasite Cuscuta campestris Yunck were also detected in some orchards. V. album was widespread among pear trees while Cuscuta campestris Yunck was detected only in suckers emerged in apple and cherry orchards. According to density A. retroflexus has received the first order with 76 plant/m2, following C. album with 54 plant/m2, Lactuca serriola with 27 plant/m2, D. stramonium L. with 24 plant/m2, Portulaca oleracea with 21 plant/m2
Show more [+] Less [-]Using Generalized Procrustes Analysis for Evaluation of Sensory Characteristic Data of Lamb Meat Full text
2019
Gazel Ser
Generalized Procrustes Analysis (GPA) is a multivariate statistic method that is used at the evaluation of sensory analyses in the food industry. GPA provides benefit in terms of decreasing the difference between the panellists and bringing the data obtained from different panellists together. In this study, the aim was to determine the effect of a pre-slaughter fasting period on sensory characteristics of lambs fed with different rations using GPA. Semi-trained panellists formed from twenty-six persons were requested for evaluation of the meat samples such as tenderness, juiciness, flavour and overall liking rated on a scale of 1(extremely dislike) to 9 (extremely good). The first two factors obtained by GPA explained 66.74% of total variability. As a result of the analysis, it was determined that 12 h and 24 h fasting of lambs fed barley supplemented with alfalfa hay were less preferable when compared to lambs fed alfalfa hay only. In addition, lambs in both groups with 48 h fasting were preferred less by the panellists. In conclusion, GPA analysis provides useful data concerning the sensitivity of each panellist in a sensory panel test.
Show more [+] Less [-]Molecular Detection and Characterization of New Emerging Viruses by PCR Analysis in Hatay and Tekirdag Vineyards Full text
2019
Hamide Deniz Kocabağ | Kadriye Çağlayan | Mona Gazel
The improvements on the next generation sequencing or high-trough output technologies allowed the discovery of several unknown viruses in grapevines and also in other plants . The most important new emerging grapevine viruses were identified as Grapevine pinot gris virus (GPGV), Grapevine syrah virus 1 (GSyV-1), Grapevine red blotch-associated virus (GRBaV) and Grapevine roditis leaf discoloration virus (GRLDaV). The aim of the present study was to investigate the occurrence and characterization of these viruses in Tekirdag and Hatay viticulture production areas by PCR and DNA sequencing analyses. Totally 191 and 111 grapevine samples showing both virus-like symptoms and symptomless were collected from Tekirdağ and Hatay provinces, respectively. Among the tested samples GPGV and GSyV-1 were detected in both local and imported cultivars by the infection rate of 43.62 % and 1.04% in Tekirdağ , respectively. In Hatay provice, only GSyV-1 was detected by the infection rate of 0.9 % and all tested samples were negative for GPGV, GRBaV, GRLDaV. RT-PCR results showed that DNA fragments of 411 bp, 302 bp and 618 bp corresponding to the part of the coat protein (CP), movement protein (MP) and the replicase genes of GPGV were successfully amplified in Tekirdağ samples. All PCR products of GPGV were directly sequenced on both strands. All the nucleotide sequences of CP, MP and 5’ UTR and N-terminus of replicase genes shared the highest sequence identity with different GPGV isolates deposited in Genbank
Show more [+] Less [-]Nutritional and Health Consequences of Bauhinia variegata Full text
2019
Muhammad Yasir Naeem | Senay Ugur
Public health around the globe is still under the major threats and of some serious infectious diseases though a marvelous progress carried out in the field of human medicines. Therefore, use of products from natural sources as medicinal agent probably antecede in human history. The advancement and knowledge of various useful plants and their properties, functions and its use over synthetic drugs has increased in recent years. Bauhinia variegata L. (Kachnar) is an ornamental flowering plant within the Leguminosae family. Hairy branches of the plant are widely used in various traditional remedies to cure vast range of disease infections. Several plant portions, like roots, stem and stem bark, leaves, buds and flowers, are not only popular in different disease treatment but also useful in the manufacture of fibers, gum and to conserve the nature. The phytochemical screening exposed that B. variegata consist of various important secondary metabolites like flavonoids, terpenoids, cardiac glycosides and steroids, saponins and tannins compounds that are appropriate to be used in the treatment of various biological activities, such as antibacterial, antidiabetic, antitumor, antimicrobial, anticancer, antiulcer, anticarcinogenic effects, anti-inflammatory, nephro and hepatoprotective effects and wound healing effect. The current review is to demonstrate the medicinal, nutritional and biological importance of B. variegata as multidimensional effects on various diseases.
Show more [+] Less [-]Fındık Üreticilerinin Tarım Sigortası Yaptırmaya Yönelik Davranışları ve Bunu Etkileyen Faktörlerin Belirlenmesi: Giresun İli Bulancak İlçesi Örneği Full text
2019
Hayriye Sibel Gülse Bal | Rüveyda Yüzbaşıoğlu | Esra Kaplan
Bu araştırmada amaç, Giresun İli Bulancak İlçesi fındık üreticilerinin tarım sigortası yaptırma durumları ve fındıkta sigorta yaptırmayı etkileyen bazı faktörlerin belirlenmesidir. 138 üretici ile çalışılmıştır. Bölgede fındık üretim alanlarında don, dolu ve rüzgârdan dolayı sık sık ürün kayıplarıyla karşılaşılmaktadır. Araştırmaya dâhil olan fındık üreticilerinin %97,83’ü doğal afetle karşılaşmış ve kayba uğramıştır. Üreticilerinin %84,78’i tarım sigortası hakkında bilgi sahibidir. Ancak çoğu (%60,87) tarım sigortası yaptırmamaktadır. Bu araştırmada tarım sigortası yaptırmada etkili olan faktörler Binary Logit analizi ile test edilmiştir. Üreticilerin sigorta yaptırmada istatistiki olarak olası etkili olan faktörler ise; üreticilerin sahip oldukları arazi büyüklüğü, yıllık tarımsal gelirleri, sürekli afet riski ve maliyetlerin yüksek olma durumu olarak belirlenmiştir.
Show more [+] Less [-]Plant Growth, Yield and Sprout Quality in Brussels Sprouts Sowed in Different Periods in Çukurova Region Conditions Full text
2019
İbrahim Burak Yılmaz | Nebahat Sarı
In this study, plant growth, yield and sprout quality were investigated by planting at three different periods in three different Brussels sprout varieties in Çukurova Region. In this research, the effects of four different sowing and planting times on plant height (cm), main shoot diameter (mm), leaf number (number/plant), yield (g/m2), sprout weight (g), sprout diameter (mm), sprout height (mm), vitamin C (mg/100 g), antioxidant capacity [μmol trolox equivalents (TE)/g] and total phenolic content [mg gallic acid equivalent (GAE)/100 g] were investigated in Franklin Fı, Maximus Fı and Divino Fı Brussels sprout varieties. According to the research results; Maximus Fı variety was found to be longer, thicker and more leafy than the other varieties in terms of plant height, main shoot diameter and number of leaves. Based on yield, Maximus Fı variety was recorded to be more efficient (584.88 g/m2) than the other varieties (367.97 g/m2 and 259.82 g/m2 in Franklin Fı and Divino Fı, respectively). Maximus Fı was superior than the other Brussels sprout varieties, Franklin Fı and Divino Fı in terms of sprout weight, sprout diameter and sprout height. When the planting times were evaluated, it was recorded that the 1st period was more suitable in terms of both yield and sprout characteristics compared to the other periods. In the vitamin C content, antioxidant capacity and total phenolic content, Franklin Fı was showed higher rates than the other varieties and more nutrient content was detected in the late plantings.
Show more [+] Less [-]Testing of Reproducibility and Consistency of Commonly Used Five Different Antioxidant Capacity Methods on Turnip Juice Full text
2019
Metin Konuş | Can Yılmaz | Nizamettin Özdoğan | Doğan Çetin | Nurhan Didem Kızılkan | Abdussamet Kayhan
Antioxidants, usually contain phenolic groups, molecules may prevent the formation of free radicals or by blocking the radicals to prevent damage to the cell. Antioxidant molecules can be produced by the body or taken into the body through foods. Today, there are many different methods used to measure antioxidant capacity. In these methods, measurements are usually carried out by adding the tested substances into a solution containing free radicals. These methods can give different results depending on the characteristics of the reactant used. In this study, the reproducibility and consistency of five different antioxidant capacity methods (DPPH, ABTS, galvinoxil, phosphomolybdenum and FRAP) in commercially available and homemade turnip samples were tested. At the end of the study, antioxidant activities of the tested samples were evaluated. According to the evaluation of the antioxidant activities (from higher to lower) of these samples, the results of, DPPH, galvinoxyl, phosphomolybdenum and FRAP methods were very similar. However, ABTS method results showed difference compared to other method results. As a result of study, reproducibility and consistency of results of DPPH, galvinoxyl, phosphomolybdenum and FRAP methods were determined.
Show more [+] Less [-]Genetiği Değiştirilmiş Organizmaların Tespiti ve Ölçümünde Kullanılan Farklı Gerçek Zamanlı PCR Kimyasallarının Karşılaştırılması Full text
2019
Leyla Bener | Mustafa Ersal | Berkant İ. Yıldız
Gerçek zamanlı kantitatif polimeraz zincir reaksiyonu (q-PCR), hem gen ekspresyonu analizinde hem de rutin Deoksiribo Nükleik Asit (DNA) ölçümünde nükleik asit miktarının belirlenmesi için kullanılan ileri moleküler bir yöntemdir. Gıda ve yem ürünlerinde genetiği değiştirilmiş organizma (GDO) kalıntıları için etiketleme eşiğinin Japonya’da %5, Avrupa Birliği’nde ise %0,9 olduğu göz önüne alındığında, doğru bir ölçüm metodu şarttır. GDO bileşenlerinin tespiti, kesin miktar tayini ve besin matrislerinde eser miktardaki kalıntısının tespit edilmesi q-PCR’da mümkündür. Bu amaçla çeşitli q-PCR kimyasalları kullanılmaktadır. Bunlar; interkalasyon boyaları, primer bazlı kimyasallar ve prob bazlı kimyasallar olarak üç gruba ayrılmaktadır. Marketlerde GDO ürünlerinin artan sayısıyla birlikte, her örnek için gerçekleştirilen analiz sayısı ve bu nedenle analiz maliyetleri artmaktadır. Bunun için GDO çalışmalarında, GDO’ların varlığının miktarını belirlemede hızlı ve ekonomik olan uygulanabilir taramalar yapılabilmesi için geliştirilmiş tespit yöntemlerine ihtiyaç duyulmaktadır. Bu çalışmada, q-PCR kimyaları ekonomikliği, verimliliği ve uygulanabilirliği açısından karşılaştırılmıştır.
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