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Phenotypic and Pathogenic Characterization of Leaf Fungi of Yam (Dioscorea spp) Varieties Grown In Côte D’Ivoire
2023
Ganemtore Souleymane | Camara Brahima | Kamara Adjata | Kassi Koffi Fernand Jean-Martial | Kone Daouda
Yam (Dioscorea spp) occupies an important place in the diet of the populations of Côte d’Ivoire. It is a real source of starch and therefore generates enormous commercial potential. However, the decrease in production due to pest attacks represents a real threat to this crop. This study was conducted with the aim of improving yam production in Côte d’Ivoire. To do so, isolations carried out on yam leaves showing symptoms of foliar diseases have allowed us to identify 9 fungal genera. These were Colletotrichum sp., Fusarium sp., Pestalotiopsis sp., Pestalotia sp., Botryodiplodia sp., Aspergillus sp., Mucor sp., Curvularia sp. and Phytophtora sp. Among these fungi, the genus Colletotrichum sp. was the most isolated with a rate of 56% followed by the Fusarium and Pestalotia genera (8%). Pathogenicity tests performed on healthy leaves of two yam varieties revealed that the Dioscorea alata is more susceptible to fungi compared to Dioscorea rotundata. The largest average diameter of necrosis was caused by Pestalotiopsis sp. (5.97 cm) on the Dioscorea alata variety while the smallest was caused by Colletotrichum sp.9 on Dioscorea rotundata (0.5 cm). Combatting these fungi need to be developed for effective management of leaf diseases of yam in Côte d’Ivoire.
Show more [+] Less [-]Molecular Survey of Toxoplasma gondii Infection in Aborted Fetuses of Sheep in the Iğdır Province of Türkiye
2023
Davut Koca | Özlem Orunç Kılınç | Adnan Ayan | Fatma Ertaş Oğuz | Ali Osman Turgut | Özge Oktay Ayan
Toxoplasma gondii, an obligatory intracellular protozoan parasite, can infect a wide range of warm-blooded animals, including livestock species. T. gondii is a zoonotic protozoan parasite that affects both humans and other warm-blooded animals. The aim of this study was to detect T. gondii by using PCR in the brain tissues of 60 aborted sheep fetuses from the Iğdır Province in Türkiye. For this purpose, 60 brain tissue samples of sheep were collected within the lambing seasons of 2023 in Iğdır, Türkiye. The DNA extraction was performed using the PureLink™ Genomic DNA Mini Kit from brain samples. The PCR was performed with the appropriate primers from the obtained DNA samples. T. gondii was found in the brain (16.6%) samples of aborted sheep fetuses. According to the present study, T. gondii infection can be one of the causes of fetus abortion of sheep in Iğdır province, Türkiye. This result emphasizes the need for vigilance and preventive measures in managing this potential public and animal health concerns.
Show more [+] Less [-]Insecticidal Effect of a Natural Turkish Diatomaceous Earth Formulation on Greater Wax Moth
2023
Mustafa Güneşdoğdu | Ali Bayram | Ali Arda Işıkber | Ahmet Şekeroğlu | Hüseyin Bozkurt
In this study, the insecticidal effect of the Detech® (Turkish Diatomaceous earth) DE formulation against Galleria mellonella L. was determined. The study was conducted in a laboratory environment with materials taken from beehives produced at Muş Alparslan University in 2022. Diatomaceous earth (DE) was applied in two different forms (dust and slurry DE) and as positive control GüveSavar®, which is currently used against some pests in beekeeping. DE concentrations of 0, 3, 5, and 7 g/m2 in different exposure times were tested for the control of G. mellonella larvae. As a result of all treatments, the highest mortality occurred at 7 g/m2 dust DE concentration. Larvae (3rd stage) exposed to 7 g/m2 concentration according to dust DE mortality rates reached 100% mortality after 40 hours. According to the results of the slurry DE, the larvae exposed to the slurry diatom at all concentrations achieved 100% mortality at the end of the 96 hours. When the dust and slurry DE results were examined, the direct use of dust formulations greatly accelerated the effectiveness against larvae. The study showed very promising results, suggesting that slurry DE and dust formulations could be a new alternative control method for Greater Wax Moth. In addition, for the first time, the insecticidal efficacy of DE against the honey bee pest, the greater wax moth, was determined.
Show more [+] Less [-]Comparison of Different Extraction Methods on the Recovery Efficiencies of Valuable Components from Orange Peels
2023
Mehmet Emin Argun | Aslıhan Öztürk | Mustafa Şamil Argun
Supercritical-CO2 extraction, Soxhlet extraction, and ultrasound-assisted extraction methods were conducted in this study to recover valuable components, specifically phenolic antioxidant compounds, from orange peels. Basic operating parameters such as temperature and pressure, which affect the extraction efficiency of phenolic substances in orange peel with supercritical-CO2, were designed using the central composite design methodology. In the Soxhlet and ultrasound-assisted extraction methods, 2-hour extraction processes were carried out using ethanol at different concentrations (50%, 80% and 100%) as a solvent. Yield comparison was made by performing total phenolic content, antioxidant activity and total flavonoid content analyses in the extracts. The total phenolic content (TPC) in the extracts was determined to be 5034 mg GAE/L for supercritical-CO2 extraction at 61.5°C and 20 MPa. In comparison, Soxhlet extraction yielded a TPC of 1728 mg GAE/L, while the ultrasound-assisted extraction method resulted in a TPC of 4056 mg GAE/L. It was determined that the optimum operating parameters of supercritical-CO2 extraction were 60°C and 26.4 MPa in case all the responses were maximized. The best phenolic recovery was obtained at 100% ethanol in Soxhlet extraction and 80% ethanol in ultrasound-assisted extraction. Although supercritical-CO2 extraction is an environmentally friendly application, the recovery rate of valuable components from raw materials is lower than in Soxhlet extraction and ultrasound-assisted extraction. However, since the volume of the extracts obtained from the supercritical-CO2 extraction is small, the ratio of phenolic compounds is higher.
Show more [+] Less [-]Mapping the Dispersion Pollution Load of Animal Waste and Investigating its Environmental Effects: The Case of Karaman
2023
Yusuf Alparslan Argun | Özgür Çakmakcı
Animal wastes are not stored correctly and are used haphazardly without control in agricultural lands. As a result, it causes many irreparable environmental pollution, especially water pollution. These wastes, which are described as diffuse pollution, cause pollution of both underground and surface water resources directly or indirectly and even make them unusable. In this research, waste generation as a result of animal breeding in Karaman province, its districts, neighborhoods and villages and the effects of animal wastes on environmental pollution were evaluated with distributed pollutant load calculations. In the study, the number of 1019277 ovine and 81368 bovine in Karaman in 2022 was used. The total nitrogen (TN) produced annually by the animals has been calculated as 1,723.23 tons/year, and the total phosphorus (TP) amount is determined as 124.23 tons/year. Additionally, for large ruminant animals, the annual total amount of dry manure is 130,305.77 tons, and for small ruminant animals, it is 41,984.27 tons. To prevent environmental pollution, these wastes should be stored in closed areas in compliance with standards, and processes such as composting, drying, and biogas production should be applied. By doing so, not only can environmental pollution be mitigated but also economic value can be obtained. The proper management and utilization of these wastes have high economic potential and can contribute to sustainable development, supporting the country’s economy. In addition, this study is a source for researchers working in the field in calculating the pollution load of animal wastes and is thought to be a guide for decision makers and practitioners.
Show more [+] Less [-]The Rational Use of Oxalic Acid Against to “Varroa Destructor”; Regional Scale Pilot Scheme
2023
Fatih Yılmaz | Sedat Sevin | Gökhan Akdeniz | Seyit Hasan Öztürk | Ahmet Kuvancı | Hasan Ese | Mücahit Buldağ | Gülden Ayvaz Baykal
Varroa destructor mite poses a serious problem for the future of bee populations around the world. Today, there are many commercial drugs with the same and different active ingredients on the market to chemically control over of Varroa destructor. More frequent chemical applications for against Varroa destructor increases stress resilience, colony losses, loss of yield and residue problems in bee products. The scope of this project is aimed to determine the appropriate control method of Varroa by investigating the efficiency values of the evaporation and dropping methods of Oxalic acid. Experimental area were chosen three different apiaries. 28 colonies were determined in each apiary and equalization studies (area with brood, number of bees with bees, age of queen bees, honey, pollen, etc.) were carried out in these colonies. The determined colonies were randomly divided into 4 groups as 7 colonies. The first group is the control group, the second group is applying 2 g of oxalic acid by vaporizing, the third group is 4% oxalic acid 5 ml of sugar syrup (1:1) is dropped between the frames, and in the fourth group, the fight against a drug that is determined by the beekeeper in the market without interfering with the beekeeper. In order to evaluate the data, samples were taken for four periods, before and after spraying in spring and autumn. While the varroa measurements in the group of syrup, vapor and spraying were found to be statistically less than the control group, the syrup, vapor and spraying groups were statistically similar in terms of varroa measurements. Oxalic acid syrup application showed higher efficiency in spring and autumn than vapor application. There is no statistically difference between both two-application method reveals that it can be used as an effective and safe alternative to chemical control against varroa.
Show more [+] Less [-]Screening of Promising Maize Varieties Against Maize Weevil (Sitophilus zeamais Motschulky) Under Storage Condition
2023
Sarjit Pandey | Dharma Raj Katuwal | Rashmi Aryal
The maize weevil (Sitophilus zeamais Motschulsky) causes significant quantitative and qualitative losses during storage. To identify resistant varieties of maize against this pest, an experiment was conducted in a Completely Randomized Design (CRD) with 11 varieties in free and no-choice conditions. The study measured weight loss, mean bored grain, debris, and weevil numbers at 30, 60, and 90 days. The findings showed that BG13Y-POP, Manakamana-7, and RML-19/RML-6 were the most resistant varieties, with weight loss percentages of 1.99%, 1.47%, and 1.74%, respectively, and final weevil numbers of 104, 72, and 73. Ganesh-2 and ZM-401 were the most susceptible varieties, with weight losses of 7.34% and 6.05%, respectively. The maximum debris weight was found in RML-761/RL-105 (1.98 g), while the minimum was found in Manakamana-7 (0.26 g). The highest number of bored grains was observed in Ganesh-2 (81), while the lowest number was observed in Rampur-4 (51). Similarly, ZM-401 (158) and Ganesh-2 (165) exhibited the highest weevil population, while the lowest count was found in Rampur-4 (72). Overall, using resistant varieties, such as Manakamana-7, BG13Y-POP, and RML-19/RML-6, can be an effective approach for reducing post-harvest losses from weevil infestation.
Show more [+] Less [-]Educational Venue from Design to Implementation Process; A Project by Faculty of Fine Arts, Selçuk University
2023
Mine Sungur | İbrahim Bakır
Throughout history, there has been a reciprocal relationship between humans and space. Even though there are numerous spaces covered by this ongoing relationship process, it has integrated and gained value with educational venues. Mainly because they closely monitor social, cultural, technological, and economic developments and pass on knowledge to future generations, educational spaces play a crucial role in the development of individuals as well as society. It is feasible to conclude from research on educational spaces that the physical environment has a major positive or negative impact on education. To solve the issue that the current Faculty of Fine Arts at Selçuk University could not sufficiently respond to user needs physically, it was decided to construct a new Faculty of Fine Arts building. The primary goal of the study is to design the building using user-oriented techniques that promote social interaction and showcase artistic identity throughout the design phase. A qualitative research approach, based on inspection and observations, was used in the study to gather data, documents, and reports on the topic and to ensure that the architectural programming stages advanced correctly and received ongoing feedback. Studies have confirmed the results, which show that artistic education in structures that provide users with distinct experiences is different from that provided in faculty buildings with a type plan scheme. The process, which involves collaboration between stakeholders from various disciplines and necessitates coordination, is also maintained in a coordinated fashion as a consequence of the study. Because of this, it is believed that buildings with comparable features can offer direction by offering a set of data that may also be reliable for design procedures that are prearranged.
Show more [+] Less [-]Potential Biological Control Agents against Soft Rot Diseases Caused by Pectobacteria on Some Sugar Beet Cultivars
2023
Mustafa Alparslan Umarusman | Kubilay Kurtuluş Baştaş
Sugar beet is one of the most economically important agricultural crops cultivated in many provinces of Turkey. Especially in recent years, there has been an increase in bacterial tuber rot due to factors related to climate change. In preliminary trials, soft rot disease by Pectobacterium caratovorum subsp. caratovorum (Pcc) and Pectobacterium betavasculorum (Pb) were detected predominantly in sugar beets in Central Anatolia. Today, some cultural measures and copper compounds are used against soft rot agents in sugar beet, but successful results cannot be obtained in preventing the disease. In this study, a total of 270 soil samples were taken from the rhizosphere of 10 different fields in 3 different periods in 3 different ecologically diverse districts (Çumra, Altınekin and Seydişehir) of Konya, one of the provinces with the highest amount of sugar beet production in Turkey. As a result of the isolations, a total of 3064 bacterial isolates were purified and 262 of them showed antibacterial activity against Pcc and Pb in vitro conditions. In addition, 15 antagonist bacteria with the highest inhibitory effect on the development of both pathogens were tested in greenhouse conditions, and according to the results obtained from here, 3 antagonists with the highest effect were tested in field conditions in the cultivation areas of 3 different districts named above. Biochemical, morphological and molecular diagnoses of antagonist bacteria with high efficacy were made. According to the results obtained, it has been concluded that rhizospheric bacteria with antagonistic effect have a success rate of 33-90% against Pcc and Pb pathogens, and that the biological products to be prepared in future studies can be used in ecological, climate friendly and within sustainable agricultural practices in sugar beet production areas.
Show more [+] Less [-]Göller Bölgesi’nde Doğal Olarak Yetişen Prangos platychlaena (Endemik) Uçucu Yağının Kimyasal Kompozisyonu
2023
Arif Şanlı | Tahsin Karadoğan | Fatma Zehra Ok
Bu çalışma, Türkiye’nin Göller Bölgesi’nde farklı lokasyonlarda yabani olarak yetişen Prangos platychlaena (endemik) bitkisinin meyvelerinin uçucu yağ oranını ve bileşenlerini belirlemek amacıyla yürütülmüştür. Türe ait meyve örnekleri iki farklı lokasyondan (Isparta, Sütçüler/Çandır ve Burdur, Bucak/Kızılkaya) sarı olum döneminde toplanmış, meyvelerin uçucu yağları hidrodistilasyon cihazı ile elde edilirken, uçucu yağ bileşenleri ise GC-MS cihazı kullanılarak belirlenmiştir. Çandır lokasyonunda yetişen bitkilerde meyve uçucu yağ oranı%0,16±0,02, Kızılkaya lokasyonunda ise%0,25±0,06 olarak belirlenmiştir. Çandır lokasyonundan alınan meyvelerin 54 bileşenden, Kızılkaya lokasyonundan alınan meyvelerin ise 44 bileşenden oluştuğu belirlenmiş, türün meyvelerinde toplam 73 farklı bileşen tespit edilmiştir. Uçucu yağı oluşturan bileşenler ve oranları lokasyonlara göre kalitatif ve kantitatif varyasyonlar göstermiştir. Her iki lokasyonda da uçucu yağı oluşturan ana bileşenler germacrene-D (%17,08-%20,24), β-bisabolene (%7,53-%17,83), β-copaene (%0,92-%11,70), caryophyllene oxide (%6,23-%6,30), β-farnesene (%3,21-%5,66), δ-cadinene (%3,50-%3,87) ve ledane (%3,35-%3,22) olarak tespit edilmiştir. Çalışmada türün yetiştiği bölgenin ekolojik faktörlerinin, özellikle rakımın uçucu yağ oranı ve bileşenleri üzerinde önemli bir etkiye sahip olduğu anlaşılmıştır.
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