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Analysis of Clade V MLO Gene Expressions in Hazelnut Leaves upon Exposure to Powdery Mildew Full text
2022
Ulku Baykal | Kadriye Özcan
Powdery mildew affecting European hazelnut Corylus avellana L. trees in Turkey is caused by the obligate biotrophic fungus Erysiphe corylacearum. This fungal disease causes significant economic losses by reducing the yield and quality of hazelnuts. Loss-of-function mutations in the mildew resistance locus o (MLO) gene family of many plants confer high levels of broad-spectrum resistance to powdery mildew. The proteins encoded by the genes at the MLO locus are divided into approximately seven different conserved clades. Among them, the phylogenetic clade V has been shown to be involved in PM susceptibility, as inactivation of these genes leads to long-term disease resistance in dicotyledons. In this study, we examined the temporal expression pattern of three hazelnut MLO genes, previously identified as clade V, in response to powdery mildew infection in C. avellana cv. Tombul. Leaves are the main tissue affected by the powdery mildew pathogen in hazelnut plants. Analysis of MLO expression in hazelnut leaves showed that CavMLO2 and CavMLO6 were significantly upregulated after challenge with E. corylacearum, providing preliminary evidence that they may be involved in PM susceptibility. Thus, these results present a basis for the isolation and use of relevant genes in plant breeding for disease resistance. Moreover, expression profiles of the clade V MLO genes are also important to identify candidate genes that need to be silenced or edited for future molecular studies to obtain resistant hazelnut varieties.
Show more [+] Less [-]The Effects of Some Nitrogen and Phosphorus Levels on Grain Yield and Some Yield Components of Sweet Sorghum (Sorghum saccharatum) Plant Full text
2022
Shiva - Sadighfard | Hakan - Geren
Sweet sorghum is a multi-purpose crop due to the use of all plant parts. The research was carried out under controlled conditions to evaluate the effect of fertilization management on growth, grain yield and yield characteristics of sweet sorghum grown as a second crop in Mediterranean climate conditions. The experiment was carried out two-factor randomized plot trial with 20 treatments combination of 5 nitrogen levels (0, 50, 100, 150, 200 kg N/ha) and 4 phosphorus levels (0, 50, 100, 150 kg P/ ha) with 3 replications. Some properties were investigated in the study such as, plant height, harvest index, thousand grain weight and grain yield per plant and some characteristics related to yield. The results shown that plant height of sweet sorghum increased with increasing N and P levels up to 200 kg N or 150 kg P per hectare, respectively, but increase beyond 100 kg N or P/ha was not found significant in general. Other features related to yield followed almost the same trend. Grain yield per plant of sweet sorghum increased linearly with increasing levels of N and P up to 200 kg N and 150 kg P/ha in both years. The combined application of 150 kg N + 150 kg P/ha produced highest grain yield.
Show more [+] Less [-]Factors Affecting Colony Losses in Honey Bees (Apis mellifera) Full text
2022
Halit Yücel | Kübra Ekinci | Altuğ Karaman | Halil Yaninar | Hülya Koca Karaman
Honey bees are a very important species in terms of economy, agriculture, and environment. In recent years, there has been a significant reduction in honey bee colonies in some parts of the world. Honeybee losses are not an unusual event, but there has been a significant reduction in honey bee colonies in many countries around the world. Due to the different social behaviors of honey bees, it is difficult to identify the main factors causing colony losses. According to the latest research, colony losses are mainly caused by parasites, diseases, bee keeping practices, and bee management including reproduction, changes in climatic conditions, agricultural practices and pesticide use, pesticides, nutrition, and beekeeping practices. In this review, the structure of prebiotic, probiotic, climate change, and vitollegen, which causes colony losses, is emphasized, and the potential solutions of these factors that will shed light on colony losses in honey bees from a different point of view are emphasized. In addition, bibliometric analysis was performed using the SCOPUS database to emphasize the importance of probiotic microorganisms and vitellogen.
Show more [+] Less [-]Investigation of TLR2 -EcoRV, NOD2- BsaHI ve IFNγ- HphI allele frequency distribution among Turkish Native Cattle Breeds Full text
2022
Yasemin Öner | Nezih Ata | Mustafa Karabaş | Onur Yılmaz
Changing climatic conditions and growing population size lead take precautions against potential risks. Researchers who study in the agricultural field, including animal production, focus on finding out variants and breeds resistant to diseases and environmental stress. Detecting gene regions that affect resistance to diseases and environmental stress might increase the performance of the selection in favor of these traits. Due to these reasons in this study, five Turkish native cattle breeds were investigated for SNPs belonging to TLR2, NOD2, and IFNγ (using EcoRV, BsaHI ve HphI enzymes, respectively) reported to be associated with paratuberculosis previously. In total, 169 animals were analyzed from Native Southern Yellow (NSY= 29), East Anatolian Red (EAR=35), Anatolian Grey (AG=36), South Anatolian Red (SAR=34), and Native Black (NB=35). The genotype and allele frequencies were calculated with the PopGene32 program, and Khi square test was performed to determine whether the populations were in Hardy-Weinberg equilibrium (HWE). While at TLR2- EcoRV and NOD2- BsaHI loci, two alleles (A and C, C and T, respectively), IFNγ- HphI locus was found to be monomorphic for the G allele. For TLR2- EcoRV locus, the C allele was found as predominant except NB breed, and the C allele was predominant for all breeds at NOD2- BsaHI locus. However, in all breeds, NOD2- BsaHI locus was at HWE, for TLR2- EcoRV NSY and SAR breeds showed derivation from HWE.
Show more [+] Less [-]Sustainable Agroforestry for Soil Chemical Properties Improvement and Nutrients Availability in Agriculture Landscape around Cyamudongo Isolated Forest, Rwanda Full text
2022
Concorde Nsengumuremyi | Eberhard Fischer | Donat Nsabimana | Marie Chantal Zaninka | JMV Senyanzobe | Bertrand Uwimana
The protected areas of Rwanda are facing various challenges resulting from the anthropogenic activities of the surrounding communities, especially in the adjacent area to Cyamudongo isolated rain forest, which results in soil degradation. Therefore, this study aims to broaden current knowledge on the impact of sustainable Agroforestry (AF) on soil-selected chemical and physical properties. To understand this, the permanent sample plots (PSPs) were established mainly in the designed four transects of four km long originating on the boundary of the Cyamudongo isolated rain forest following the slope gradient ranging from 1286 to 2015 m asl. A total number of 73 PSPs were established in the Cyamudongo study area. The Arc Map GIS 10.4 was used to design and map the sampling areas while GPS was used for localization of plots centers. Statistical significance was analyzed through R-software. The recorded soil pH means value across in Cyamudongo study area is 4.2, which is strongly acidic. The tests revealed that the soil pH, C, N, C: N ratio, OM, NH4+, NO3-+NO2-, PO43-, and CEC were significantly different in various soil depths. The pH, N, C: N ratio, CEC, NH4+, PO43-, and Al3+ showed a significant difference across land uses whereas the C and NO3-+NO2- did not show any statistical difference. All tested chemical elements showed a statistical difference as far as altitude ranges are concerned. The only NH4+, PO43-, and CEC showed significant differences with time whereas all other remaining chemical elements did not show any statistical significance. The soil pH was very strongly correlated with CEC, Mg, and Ca in cropland (CL) whereas it was strongly correlated in both AF and natural forest (NF) except for Mg, which was moderately correlated in AF. Furthermore, its correlation with K was strong in CL, and moderate in AF while it was weak in NF. Finally, the pH correlation with Na was weak in both AF and CL whereas it was negligible in NF.
Show more [+] Less [-]Free Radical Scavenging and Antinociceptive Activities of the Aqueous Extract from Matricaria chamomilla L. Flowers. Full text
2022
Chahrazed Kaoudone | Fatima Benchikh | Cherif Abdennour | Hassiba Benabdallah | Walid Mamache | Smain Amira
Background: Matricaria chamomilla L. (M. chamomilla) is a famous medicinal plant distributed worldwide. It is widely used in traditional medicine to treat all kinds of diseases, including infections, neuropsychiatric, respiratory, gastrointestinal, and liver disorders. It is also used as a sedative, antispasmodic, antiseptic, and antiemetic. Our aims in this study was thus to quantify the phenolic, flavonoids contents in the flower of this plant, and also to evaluate the in vitro antioxidant potential and the in vivo analgesic activity. Methods: The total phenolic and flavonoid contents of the plant aqueous extract (MCAqE) were estimated using the Folin–Ciocalteu and AlCl3 colorimetric methods, respectively. However, DPPH method was used to evaluate the in vitro antioxidant activity. Analgesic activity was tested by acetic acid induced writhing model in mice. Results: Quantitative determination of total polyphenols and flavonoids revealed that this extract contained 158.41±1.6 mg gallic acid equivalent/g of dry extract and 37.06±0.56 mg quercetin equivalent/g of dry extract, respectively. The antioxidant activity of the plant extract was important (IC50=3.08±0.25 mg/mL). MCAqE extract, at 400 mg/kg, showed analgesic activity (39.60±8.70%) against acetic acid induced pain in mice while the standard reference drug Diclofenac sodium exhibited 90.44±2.80% activity at 10 mg/kg dose.
Show more [+] Less [-]Determination of Fatty Acid Content of V. opulus Grown in Sivas, Kayseri and Yozgat Full text
2022
Alper Zöngür
The common name for Viburnum opulus in Türkiye is "gilaburu". Despite its bitter and acrid taste, it is consumed as fruit juice or products such as jam, marmalade, jelly. They have antioxidant properties as they contain high levels of phytocompounds such as anthocyanin, phenolics, triterpenoids and vitamins. In the study, the fruits of V. opulus grown in Sivas, Kayseri and Yozgat villages were found to contain palmitic acid (C 16:0), oleic acid (C 18:1), linoleic acid (C 18:2), myristic acid (C 14:0), palmitoleic acid (C 16:1), stearic acid (C 18:0), heptadesanoic acid (C17:1), eicosenoic acid (C 20:1), eicosadienoic acid (C 20:2), lauric acid (C 12: 0), margaric acid (C 17:0), pentadecanoic acid (C 15:0), caprylic acid (C 8:0) and gincoloic acid (C 15:1) were examined. In the study, it was observed that the dominant fatty acids were palmitic acid (C 16:0), oleic acid (C 18:1) and linoleic acid (C 18:2). Similarly, in the examples, lauric acid (C 12:0), margaric acid (C 17:0), pentadecanoic acid (C 15:0), caprylic acid (C 8:0) and gincoloic acid (C 15:1) found in trace amounts.
Show more [+] Less [-]The Effect of Drying Temperatures on Effective Diffusion-Activation Energy, Quality and Drying Kinetics of Rosehip Fruit of “Star” Type Full text
2022
Burcu Aksüt | Hakan Polatcı
Rosehip fruit, which is widely and naturally grown in many European countries as well as in Rosehip fruit, which is widely used in Turkey as well as in European countries and grows naturally, is a very rich species in terms of vitamin C value. Due to its benefits to human health, its consumption is increasing and its use in different forms is becoming widespread. In this study, the colour, effective diffusion-activation energy, phytochemical properties and thin-layer drying patterns of rosehip fruit of the ‘Yıldız’ variety were investigated after drying in a convective dryer at different temperatures (40, 50, 60 and 70°C). In the study, it was determined that 70°C temperature was statistically more suitable in terms of measured and calculated colour values. It has been determined that the effective diffusion value is affected by the drying temperature and the increase in temperature also increases the effective diffusion value. It has been determined that the effective diffusion value varies between 1.35×10-7-5.92×10-6 m2/sec. The activation energy value of dried rosehip samples was calculated as 69,41 kJ/mol. When the chemical analysis values were examined, when the SÇKM, total phenolic, total antioxidant substance and vitamin C values were evaluated together, it was found that the most appropriate drying temperature was 70°C statistically. Among all drying models, Midilli-Küçük and Yağcıoğlu models (R2: 0.9999) estimated the drying data best.
Show more [+] Less [-]An Overview on The Use of Lemna minor L. in the Field of Health and Nutrition Full text
2022
Eda Sönmez Gürer | Evren Algın Yapar
Lemna minor L. is an aquatic plant that is resistant to hard conditions, is a fast growing, rapidly multiplying vascular floating macrophyte, and is widely distributed in wetlands in our country and around the world. Lemna minor L., also known as duckweed, contains essential nutrients such as proteins, carbohydrates and fats, as well as various secondary metabolites that are beneficial for humans. Therefore, it is a remarkable source of alternative food. In addition to its traditional medical use, it is thought that taking into account in the development of herbal medicines and cosmetics today in the light of scientific studies will be beneficial in terms of developing value-added products and bringing them into the economy. In this review, studies and articles on the usability of Lemna minor L. in the field of health and nutrition are reviewed and presented with an overview.
Show more [+] Less [-]The Determination of Tolerances of Some Cotton Genotypes Against Wilt Disease (Verticillium dahliae Kleb.) Under The Ecological Conditions of Diyarbakir Full text
2022
Nurettin Baran | Mehtap Andırman | Flavien Shimira | Fildaus Nyirahabimana | Mefhar Gültekin Temiz
Verticillium wilt disease in cotton is a soil-borne disease that causes significant yield losses, and it is therefore important to identify disease-resistant genotypes. Current study was carried out in the experimental field of the Faculty of Agriculture, Dicle University (Diyarbakir) in the year 2020 to determine the tolerance levels of some cotton genotypes which contain different characteristics to wilt disease (Verticillium dahliae Kleb.). The experiment area has been naturally infected with Verticillium wilt pathogens. A total of 124 cotton genotypes, including four controls (DP-499, Edessa, ST-468, ES-1), were arranged in five blocks according to the Augmented Design. The indices of stem cross-section and the leaf-disease severity were found significant. The leaf-disease severity varied between 0.12-3.09 at 50-60 % at boll opening period, whilst stem cross-section values ranged between 0.36-2.30. Moreover, a positive correlation was found between the indices. In conclusion, G7, G12, G21, G22, G23, G41, G51, G55, and G101 were determined as tolerant genotypes to Verticillium wilt in terms of examining two parameters.
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