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Antibacterial Activity and Chemical Composition of Essential Oils from Some Galium (Rubiaceae) Species Against Pathogenic Bacteria Full text
2017
Filiz Yağız | Rifat Battaloğlu | Sedef İlk | Ahmet Savran
In this work, chemical composition and antimicrobial activity of the essential oils of Galium incanum, Galium dieckii ve Galium aladaghense were firstly reported. The essential oils were obtained from the all parts of the plant by hydrodistillation and analyzed by using GC-MS. Antimicrobial activity of synthezied essential oils was carried out against 5 pathogen bacteria Escherichia coli (E. coli) ATCC 25922, Staphylococcus aureus (S. aureus) ATCC 25923, Pseudomonas syringae pv. tomato (P. syringae) DC300, Salmonella enterica serotype Typhmurium (S. typhmurium) SL 1344 and Streptococcus mutans (S. mutans) ATCC 25175. According to the results, it was determined that isolated essential oils comprised of 61 compounds. Compounds of essential oils included that structure monoterpene (8.2%), monoterpenoid (14.75%) and sesquiterpene (14.75%). Unclassified compounds have been identified as other compounds. From the antimicrobial activity was observed that the isolated essential oil from Galium incanum, Galium dieckii ve Galium aladaghense exhibited a potent inhibitory effect against all gram negative and gram positive bacteria with diameter of inhibition zones ranging from 4.3 to 12.3 mm. Essential oil of Galium aladaghense indicated that high antimicrobial activity on all bacteria than Galium incanum and Galium dieckii.
Show more [+] Less [-]Samanlarda Biyolojik Muamelelerle Lignoselüloz Kompleksin Sindirilebilirliğinin Artırılması Full text
2017
Aydan Atalar | Nurcan Çetinkaya
Samanların hücre duvarında bulunan lignoselüloz kompleksin parçalanması ile selüloz ve hemiselüloz gibi rumen fermantasyonuyla sindirilebilir fraksiyonlar elde edilir. Rumende sindirilmeyen ligninin biyoteknolojik metotlarla parçalanarak samandan yararlanılabilirliğin artırılması son yıllarda hayvan beslemecilerin odak noktası olan alanlarından birisi olmuştur. Lignoselüloz kompleksin biyolojik metotlarla muamelesinde bakteriler, mantarlar ve bu mikroorganizmalardan elde edilen enzimler kullanılmaktadır. Bakteri muamelesinde Mycobacterium, Arthrobacter ve Flavobacterium türü bakterilerin lignini parçalayabilme özelliğinden yararlanılmaktadır. Enzim muamelesi etkili olmasına rağmen yüksek maliyet nedeniyle uygulamada yer bulamamıştır. Mantar muamelesinde beyaz, kahverengi ve yumuşak çürüme yapan 3 tür mantar kullanılmaktadır. Kahverengi çürükçül mantarlar tercihen selüloz ve hemiselüloza saldırır, ancak lignini parçalayamaz. Beyaz çürükçül mantarlar lignine saldırarak lignol bağları ve aromatik halkayı parçalarlar. Beyaz çürükçül mantarlar selülaz, ksilanaz gibi hidrolitik enzimlerle polisakkaritleri ve lignin peroksidaz ve lakkaz gibi oksidatif ligninolitik enzimlerle lignini parçalarlar. Lignoselülozik materyalleri en iyi parçalayabilen mikroorganizmaların mantarlar olması ve maliyetin düşük olması nedeniyle özellikle beyaz çürükçül mantarların uygulama potansiyeli bulunmaktadır. Bu bildiride biyoçeşitliliğin sağladığı avantajla biyolojik metotlarla samanların sindirilebilirliğinin artırılması tartışılacaktır.
Show more [+] Less [-]Investigate of Physicochemical and Microbiological Quality of Yenice-Davutköy (Çanakkale) Irrigation Ponds Full text
2017
Kahraman selvi | Seda Özdikmenli Tepeli | Burcu İleri | Ramazan Yıldız | Mehmet Ali Yücel
The aim of this study is to determine seasonally water quality characteristics irrigation ponds. Surface water samples were collected from three different sampling points in Yenice and Davutköy irrigation ponds (NW Turkey) for four different seasons. The parameters of dissolved oxygen, pH, salinity, electrical conductivity, chemical oxygen demand, biochemical oxygen demand, ammonium, nitrite, nitrate, total hardness, chloride and sodium adsorption rate (SAR) were investigated for Yenice and Davutköy irrigation water quality. According the national and international water quality standards, physicochemical parameters were determined under permissible level. In addition, sampling ponds are located C1−S1 class of ABD salinity diagrams. This result means that salinity and sodium values of water are suitable for ecological system and can be using for irrigation. Furthermore, the microbial quality of irrigation water for the ponds were determined that rate of mesophyll and psychotropic bacteria are connected with temperature. It is detected that the term of low temperature is high level of psychotropic bacteria and the term of high temperature increase the number of mesophyll bacteria. Pearson correlation analysis between the results of the statistical psychotropic aerobic count and temperature significant was found a strong negative correlation (r= -0,635). Fecal pollution indicator as the number of E.coli stations in Davutköy pond in the summer sampling were determined 110 cfu/100ml and 50 cfu/100ml, respectively. E.coli was not detected other stations and sampling time. Finally, according to obtained analysis results are determined that water qualities for Yenice and Davutköy ponds are suitable for agriculture activities.
Show more [+] Less [-]Effect of Soaking and Boiling on Anti-nutritional Factors, Oligosaccharide Contents and Protein Digestibility of Newly Developed Bambara Groundnut Cultivars Full text
2017
Olaposi Adeleke | Oladipupo Qudus Adiamo | Olumide Samson Fawale | Gbeminiyi Olamiti
Newly developed Bambara groundnut (Vigna subterranea L.) seeds (Accessions No: TVSU 5 – Bambara Groundnut White (BGW) and TVSU 146 – Bambara Groundnut Brown (BGB)) were collected from International Institute of Tropical Agriculture (IITA), Nigeria, planted and harvested. The effects of processing methods (soaking and boiling) on anti-nutritional factors and oligosaccharides content and protein digestibility of BGW and BGB compared with Bambara groundnut commercial (BGC) seeds were investigated. Soaking and boiling significantly reduced the anti-nutritional factors of the samples and the effect increased as processing time was elongated. Sample BGC had lower anti-nutritional factors than BGW and BGB after soaking for 48 h. Tannin contents of the samples were reduced drastically by 99 % throughout the soaking periods. Greatest loss in raffinose level was observed in BGB (59%) and BGW (50%) after boiling for 60 min compared with BGC (43%). The loss in stachyose content of the samples varies with processing and BGC (59%) had greatest loss after boiling for 60 min while soaking for 48 h reduced that of BGB and BGW by 57 and 35%, respectively. Boiling for 60 min increased the in vitro protein digestibility of BGB (89.34 %) compared with BGW (87.48%) and BGC (82.89%). Overall, the results demonstrated that soaking and boiling of newly developed Bambara groundnut seeds could improve the nutritive quality of the seeds.
Show more [+] Less [-]Analyses of Dynamics in Dairy Products and Identification of Lactic Acid Bacteria Population by Molecular Methods Full text
2017
Aytül Sofu
Lactic acid bacteria (LAB) with different ecological niches are widely seen in fermented meat, vegetables, dairy products and cereals as well as in fermented beverages. Lactic acid bacteria are the most important group of bacteria in dairy industry due to their probiotic characteristics and fermentation agents as starter culture. In the taxonomy of the lactic acid bacteria; by means of rep-PCR, which is the analysis of repetitive sequences that are based on 16S ribosomal RNA (rRNA) gene sequence, it is possible to conduct structural microbial community analyses such as Restriction Fragment Length Polymorphism (RFLP) analysis of DNA fragments of different sizes cut with enzymes, Random Amplified Polymorphic DNA (RAPD) polymorphic DNA amplified randomly at low temperatures and Amplified Fragment-Length Polymorphism (AFLP)-PCR of cut genomic DNA. Besides, in the recent years, non-culture-based molecular methods such as Pulse Field Gel Electrophoresis (PFGE), Denaturing Gradient Gel Electrophoresis (DGGE), Thermal Gradient Gel Electrophoresis (TGGE), and Fluorescence In-situ Hybridization (FISH) have replaced classical methods once used for the identification of LAB. Identification of lactic acid bacteria culture independent regardless of the method will be one of the most important methods used in the future pyrosequencing as a Next Generation Sequencing (NGS) techniques. This paper reviews molecular-method based studies conducted on the identification of LAB species in dairy products.
Show more [+] Less [-]Determination of Main Plant Sterols in Turkish Bread Wheat (Triticum aestivum L.) by GC-MS Full text
2017
Halil Erdem | Yener K. Tosun | Mohammed Akkbik | Orhan Hazer
Plant sterols are belong to triterpenes family of natural products which includes more than 200 different types of plant sterols and more than 4000 other types of triterpenes. The optimization of method, specially the derivatization step as well as the corresponding analytical validation, is the main goal of this study. The optimum temperature, time and reagent volume of derivatization step were obtained at 60°C, 60 minutes and 50 µL, respectively. A rapid and sensitive gas chromatographic–mass spectrometric method was developed and validated for quantitative analysis of the most common plant sterols (β-sitosterol, campesterol and stigmasterol) in 20 Turkish bread wheat cultivars using GC-MS-SIM. Separation of β-cholestanol (I.S), campesterol, stigmasterol and β-sitosterol was achieved on Rxi (5Sil MS) column (60 m×0.25 mm). The limits of detection for β-sitosterol, campesterol and stigmasterol were 0.074, 0.054 and 0.064 mg kg-1, respectively with RSD ≤ 0.66%. The obtained concentrations of campesterol, stigmasterol and β-sitosterol from 20 Turkish bread wheat cultivars ranged from: 15.30 to 76.02, 4.27 to 23.23 and 303.21 to 682.66 mg kg-1, respectively.
Show more [+] Less [-]A Review on Food Production with 3 Dimensional (3D) Printing Technology Full text
2017
Celalettin Değerli | Sedef Nehir El
Three dimensional (3D) printing technology, have been quite popular in recent years. It came out first in the area of material production, but now, it has been applied on the other possible fields like food production. In this review, historical period of 3D printing, 3D printer types and working principles, studies on 3D food printing until today and the raw materials used in this studies were investigated. Studies on food printing was also categorised according to food types. Also, the impacts of 3D printing technology on food sector from the point of producer and consumer and future needs were discussed.
Show more [+] Less [-]The Importance of Drying for Valorization of 2-Phase Olive Pomace Full text
2017
Ulaş Baysan | Mehmet Koç | Figen Ertekin
With starting healthy consumption awareness in people throughout world, olive oil demand has increased and it is expected that this demand will increase day by day. As a result of increase in the demand for olive oil, the rise in amount of olive pomace that emerges after olive oil production is evident. The differences in olive oil production methods result in varied wastes in terms of property and quantity. Olive mill waste water and olive pomace possessing 35-40% moisture come out in 3-phase system while only olive pomace possessing 60-70% moisture comes out in 2-phase system. The quantity and pollution degree of waste water coming out in 3-phase system are considerably high from 2-phase system. Recycling of 2-phase olive pomace containing also olive mill waste water, which is highly harmful for environment and is generally discharged to nature without any treatment, is considerably important by processing. This review gives information about the necessity of drying of olive pomace and related studies with this subject.
Show more [+] Less [-]Willingness to Pay Additional Water Rate and Irrigation Knowledge of Farmers in Dinar Karakuyu Irrigation Areas in Turkey Full text
2017
Mevlüt Gül | Kutlan Uzunkaya
Water which has become commodity product which is an important product today. Turkey is not a water rich country. In this study, agricultural enterprises in the field of Irrigation Project in Dinar Karakuyu which was implemented in 1992 by DSI. The study analysed which factors affect the willingness to pay additional irrigation water rate with the help of logit model and the irrigation knowledge of farmers was determined by Likert scale. Dinar Karakuyu irrigation network has begun to lose the function in the region. It was supposed 100% irrigation rate but decreased by approximately 9% today. In this context, DSI (General Directorate of State Hydraulic Works) plans to rehabilitation work in the same area. The main material of this study was data obtained from 67 agricultural enterprises through a survey covered by the Irrigation Rehabilitation Project in the province of Afyonkarahisar Karakuyu Dinar. The data was gathered with the help of questionnaires which were answered by farmers in Karakuyu Dinar region. The results indicated that 74.6% of farmers were willingness to pay additional water charge. The data were statistically analysed with the use of the logit model. The model results show that agricultural income, farmers’ educational level, computer ownership, attendance of agricultural training activities, family size and agricultural experience were positive factors affect farmers’ willingness to pay additional water fee.
Show more [+] Less [-]Effects of Some Environmental Factors on Milking Time Milk Yield in Red Holstein Cows Full text
2017
Atakan Koc
The purpose of this study is to investigate the environmental factors on the milking time milk yield of Red Holstein cows. For this aim, 172,826 morning milk yield (MMY) and 172,771 evening milk yield (EMY) data collected between 2001 and 2010 from a dairy farm in Aydin Province, Turkey were used. The THI is over 72 from June to August in the region. The effects of calving month, calving year, parity, lactation month and milking month on MMY and EMY were found to be statistically significant. For every month, year and parity, the MMY means were higher than those of the EMY. The peak daily milk yield was determined on the 46th day of lactation (31.7±0.013 kg). For parity, the highest milk yield means were detected for the third parity: 13.69±0.023 kg for MMY and 11.70±0.021 kg for EMY. In conclusion, taking precautions to protect the cows from heat stress especially for the cows calved in summer and keeping the milking interval equal for whole year would help increasing the milk yield.
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