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PCR Based Detection of Shiga Toxin Producing E. coli in Commercial Poultry and Related Environments
2015
Homaira Anzum Himi | Md. Shafiullah Parvej | M. Bahanur Rahman | K M Nasiruddin | Wahedul Karim Ansari | Md. Mostakin Ahamed
Shiga toxin (Stx)-producing E. coli (STEC) is the most important foodborne pathogen which is the causal agent of mild diarrhea, bloody diarrhea, hemolytic-uremic syndrome (HUS) in human. The present study was designed to determine the prevalence and identification of Shiga toxin (Stx)-producing E. coli in poultry, detection of its source of infection in poultry and transmission pattern to human. For this purpose a total of 150 samples (cloacal swab-60, feed -15, water-15 and egg -60) were collected and analyzed in bacteriology laboratory by cultured in different bacteriological media followed by gram’s staining, biochemical tests and Polymerase Chain reaction (PCR). The PCR was performed by targeting 16s rRNA gene and shiga toxin producing gene in E. coli. Out of 150 collected samples, E. coli was found in 81 (54%) samples. Presence of E. coli was 100% in both feed (n=15) and egg (n=60), whereas 10% in cloacal swab (n=6). Water samples were totally free of E. coli. The stx2 gene was detected in all samples whether all samples were negative for stx1 gene. The study revealed that, poultry feed acts as a source of E. coli infection in poultry, which may be transmitted to environment and human via meat or eggs. Antibiotic sensitivity test revealed that isolated bacteria were highly sensitive to Ciprofloxacin.
Show more [+] Less [-]Study on Climatic Variation and Its Effect on Vegetable Type Soybean Genotypes at Khumaltar, Lalitpur in the Last Ten Years
2015
Santosh Raj Tripathi | Jiban Shrestha | Jagat Devi Ranjit | Reshma Neupane
Soybean (Glycine max L. Merril) is widely grown in the mid hills as intercrop with maize or in paddy bunds, while it is gaining popularity as sole crop in terai and inner terai. Mean temperature at Khumaltar during soybean growing period was mostly fluctuating; but we observed an increasing trend in temperature. Amount of rainfall was not changed dramatically but number of rainy days was decreased during study period. Rainfall during germination time increase soil moisture which also increase germination and found higher early stand. Days from sowing to 50% flowering and 90% maturity were short in the case of higher minimum temperature and low rainfall. Among the genotypes, AGS-377, AGS-378, AGS-379 and Tarkari Bhattmas-1 were more sensitive. However, seed yield decreased in the case of higher temperatures and low rainfall. Cool night temperatures and high moisture increased disease incidence in soybean which, eventually reduced yield. In last three years, plant suffered from moisture stress during early vegetative stage and high moisture during late vegetative stage which reduced seed yield and seed weight. In conclusion, we found that genotypes like AGS- 360, Sathiya and Tarkari Bhatmas-1 are very sensitive to climatic variation.
Show more [+] Less [-]The Structure, Problems and Solutions of Fruit Sapling Producing Enterprises in Hatay Province
2015
Nuran Tapkı | Faruk Emeksiz | Erdal Dağıstan
In this study, examined the structure and problems of fruit sapling producing enterprises and offers solutions in The Hatay Province. The data were obtained from 92 enterprises by the method of full count and face to face with producers. Of the 51.15% population in creating family business primary school graduates, the proportion of university graduates remained at 8.62%. The rate of agricultural engineer employment in the businesses is 2.18%. While 64.05% of the enterprisers the father's profession, this profession began taking 3.37's% of nursery education. Average potential family labour is the 4.22 male labour forces in enterprises. The family labour utilization rate was calculated as 50.71%. Of the 88.48% in fruit sapling businesses, 5.32's% in other agricultural productions and 5.22's% in non-agricultural jobs of total used men workforce. The businesses have average total land size of 25.74 acres which it has established 12 acres of fruit sapling production land. While 74.58% of the total fruit sapling land is property, 25.42's% is for rent situation. The operating capital is calculated as the average of 229.308 TL. The most common species of fruit sapling production were citrus (46.20%) and olive trees (35.29%). The problems faced by most of the farms in the region were high input prices, excessive bureaucratic procedures preventing memberships into the union, an inadequate amount of support provided by the state, production-threatening diseases of plants and difficulties in finding qualified staff in the region. The results show that enterprises need to increase their production capacity, to unite under one organization to facilitate the resolution of problems.
Show more [+] Less [-]Development of a PCR/RLB Test for Meat Source Authentication in Processed Meat and Meat Products
2015
Ibrahim Abbasi | Al-Shareef Abdel-Kareem | Matouk Imad | Akkawi Muataz
Concern over food authenticity has increased as a result of an increase in the consumption of processed foods containing meat or animal products. This raises a number of issues where the presence of pork in such foods is considered unacceptable in most Muslim and Jewish communities around the world. It also applied to the prohibition of beef consumption among Hindus. In order to ensure the absence of unwished meat products or mixing of meats from different sources in processed foods, a specific and sensitive test is essential. For this purpose we developed a molecular test based on DNA amplification by polymerase chain reaction (PCR) of the cytochrome b gene followed by reverse line blot analysis (RLB). Using this method many samples may be treated simultaneously and meat origins can easily be detected from processed foods or foods containing mixed meat sources; also, added pork components such as fat may be identified by this methodology. The PCR/RLB method is considered to be a sensitive and specific technique; it can detect one nucleotide change within the PCR-amplified DNA segment.
Show more [+] Less [-]A Research on Red Meat Consumption and Preferences: A Case Study in Tekirdağ Province
2015
Ebru Onurlubaş | Neslihan Yılmaz | Hasan Gökhan Doğan | Halil Kızılaslan
In this study, 384 persons have been subjected to questionnaire made in order to determine the red meat consumption and preferences of the people living in the central district of Tekirdağ province. In the study it was determined that all the consumers consumed red meat. According to the findings of the research, the annual red meat consumption per capita was determined to be 34.22 kg. Considering the red meat consumption of the people subjected to research, it was determined that beef meat was the most preferred kind among all the other kinds of red meat. In the study, it was determined that in red meat buying place preference consumers prefer traditional retailers such as butcher been specialized. Consumers prefer red meat due to be the most nutritious, respectively be healthy, delicious, habit and easy to access. It was determined that 47.5% of consumers participated in the study were ready to pay extra for red meat in the food safety. It was determined 75.6% people participated in the study consume more red meat if the price of red meat cheapens. A logit model was used for analyzing the factors that influence the red meat consumption of the families participating in this research. According to the logit model results, it was determined that the families’ red meat consumption amount is affected from statistical variables such as; number of family members, education level, spouse's employment status, income, cheapening of the price of red meat .
Show more [+] Less [-]The Nutritive and Feeding Value of Olive Cake for Ruminants
2015
Gürhan Keleş
The factor affecting nutritive and feeding value of olive cake (OC) was evaluated and, some suggestion was made regarding using olive cake in ruminant nutrition. It is evaluated that the nutritive value of OC can be able to support maintenance requirement of ruminant, although its nutritive value is affected by different factors. However, when taking into consideration of expense needed for preservation of OC, it is wise to use OC in nutrition after de-stoning. The crude protein, ether extract, NDF, lignin and non-fiber carbohydrates content of partly de-stoned OC (POC) was determined as 75 (56-93), 126 (68-184), 571 (443-700), 222 (174-269) and 141 (55-227) g/kg dry matter (DM). These values show that POC is a valuable source of ruminant feed. Besides, high and quality ether extract content of OC separates it from other feed and made it a special feed. Treatment with alkali increases the degradability and in vivo digestibility parameters of OC. Therefore, ensiling allowing the treatment with additives is best option to preservation of OC. Even, additional extraction of oil from POC decreases its nutritive value; studies showed that POC after additional oil extraction has still favorable nutritive value. Studies showed no adverse effect when POC substitute 20% of ration of feedlot lamb. For dairy animal, it is evaluated using POC to provide 15-20 g/kg DM oil when it substitute of 10-20% of total ration allow to produce more milk with increased fat and quality. In conclusion, de-stoning process turns OC to a valuable feed source, allows reduce feed cost, and increase animal performance and product quality. However, because nutritive value of OC affecting from very different factors, it can be propose analyze its nutritive value before using it in ruminant nutrition.
Show more [+] Less [-]A Comparative Study of Some Estimation Methods in Simple Linear Regression Model for Different Sample Sizes in Presence of Outliers
2015
Soner Çankaya | Samet Hasan Abacı
The aim of this study was to compare some estimation methods (LS, M, S, LTS and MM) for estimating the parameters of simple linear regression model in the presence of outlier and different sample size (10, 20, 30, 50 and 100). To compare methods, the effect of chest girth on body weights of Karayaka lambs at weaning period was examined. Chest girth of lambs was used as independent variable and body weight at weaning period was used as dependent variable in the study. Also, it was taken consideration that there were 10-20% outliers of data set for different sample sizes. Mean square error (MSE) and coefficient of determination (R2) values were used as criteria to evaluate the estimator performance. Research findings showed that LTS estimator is the best models with minimum MSE and maximum R2 values for different size of sample in the presence of outliers. Thereby, LTS method can be proposed, to predict best-fitted model for relationship between chest girth and body weights of Karayaka lambs at weaning period, to the researches who are studying on small ruminants as an alternative way to estimate the regression parameters in the presence of outliers for different sample size.
Show more [+] Less [-]Fixing Methods of Type in Sheep and Goat Breeding
2015
Hilal Tozlu Çelik | Mustafa Olfaz
Improvement studies are conduct to increasing of productivity native sheep breeds. This studies requires being quite comprehensive and disciplined. Improvement studies to achieve the desired characteristics and in which phase of finish is crucial. In this review, we focus on obtaining and fixing type in order to need to be implemented methods in sheep and goats.
Show more [+] Less [-]Absorption Of Dietary Lipid Components
2015
Abdulkadir Hurşit
Although the digestion and absorption of lipids that are necessary for the survival of living organisms are well known in general terms, nevertheless how different lipids to be digested, how it is distributed into the bloodstream, and how to be used by the cells, are unknown issues by most non specialist people. In recent years, knowledge of lipid digestion and absorption has expanded considerably. More insight has been gained in the mechanism of action of H + pump as a transport system in fatty acid absorption. New knowledge gained on the structure-function relationship and mechanisms of action of several lipolytic enzymes in the gastrointestinal tract. This work intends to give an up to date view of lipid digestion and absorption.
Show more [+] Less [-]Doğal Antioksidanların Sınıflandırılması ve İnsan Sağlığına Etkileri
2015
Cemal Kasnak | Recep Palamutoğlu
Gelişen ve durmaksızın ilerleyen teknoloji, çevre kirliliği, radyasyon, kontamine sular, tarım ilaçları, ağır metaller ve canlı hücrelerdeki oksijen metabolizması gibi birçok etken insan vücudunda kaçınılmaz olarak serbest radikallerin oluşumuna neden olmaktadırlar. Serbest radikaller, oksijenin oldukça reaktif formları olup, vücut hücrelerini tahrip etmektedir. Bu da kalp damar hastalıkları, kanser, katarakt, diyabet, karaciğer tahribatı ve diğer pek çok hastalığa davetiye çıkarmaktadır. Bu hastalıklara çözüm getirmek öncelikle serbest radikallerin olumsuz etkilerini bertaraf etmek ve hastalıkların oluşumunu engellemekle gerçekleşebilir. İnsan vücudunda bunu engelleyebilecek antioksidan savunma sistemi mevcut olsa da karşılaşılan çevresel etmenler bu savunma direncini düşürmekte ve çoğu kez yetersiz bırakmaktadır. Zayıflayan antioksidan savunma sistemlerimizi doğal ve dengeli beslenerek güçlendirebiliriz. Bu makalede serbest radikallerin neden olduğu hastalıklara karşı direnç gösteren en önemli endojen ve ekzojen doğal antioksidanların sınıflandırılması ve insan sağlığına etkileri incelenmiştir.
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