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Analyses of Dynamics in Dairy Products and Identification of Lactic Acid Bacteria Population by Molecular Methods
2017
Aytül Sofu
Lactic acid bacteria (LAB) with different ecological niches are widely seen in fermented meat, vegetables, dairy products and cereals as well as in fermented beverages. Lactic acid bacteria are the most important group of bacteria in dairy industry due to their probiotic characteristics and fermentation agents as starter culture. In the taxonomy of the lactic acid bacteria; by means of rep-PCR, which is the analysis of repetitive sequences that are based on 16S ribosomal RNA (rRNA) gene sequence, it is possible to conduct structural microbial community analyses such as Restriction Fragment Length Polymorphism (RFLP) analysis of DNA fragments of different sizes cut with enzymes, Random Amplified Polymorphic DNA (RAPD) polymorphic DNA amplified randomly at low temperatures and Amplified Fragment-Length Polymorphism (AFLP)-PCR of cut genomic DNA. Besides, in the recent years, non-culture-based molecular methods such as Pulse Field Gel Electrophoresis (PFGE), Denaturing Gradient Gel Electrophoresis (DGGE), Thermal Gradient Gel Electrophoresis (TGGE), and Fluorescence In-situ Hybridization (FISH) have replaced classical methods once used for the identification of LAB. Identification of lactic acid bacteria culture independent regardless of the method will be one of the most important methods used in the future pyrosequencing as a Next Generation Sequencing (NGS) techniques. This paper reviews molecular-method based studies conducted on the identification of LAB species in dairy products.
Show more [+] Less [-]Root Biomass Distribution with Root and Shoot Development at Different Growth Stages of Wild, Ancient and Modern Wheat Species
2017
Hayati Akman
This study was conducted to investigate root and shoot traits of wild, ancient and modern wheat genotypes belonging to 8 different species at long tubes under field weather conditions. It was found significant differences between genotypes with regard to root and shoot traits. The research results indicated that root biomass distribution of genotypes at GS 31 ad GS 69, respectively was found 59.2% and 56.3% in 0-30 cm, 76.7% and 71.9% in 0-60 cm. Modern wheats (Çeşit 1252, Konya 2002) and Triticum vavilovii (ancient wheat) had high root biomass distribution in top soil. In the study, species with AABBDD genomes had higher root length than those with AABB, AABBGG and AA genomes. Triticum dicoccoides, Triticum timopheevii and Triticum monococcum had lower values than other genotypes in terms of root length, crown root number, root biomass, shoot biomass and plant height at both growth stages (GS 31 and GS 69), while Triticum vavilovii and Triticum spelta (hexaploid) took part in the front. Accordingly, Triticum vavilovii and Triticum spelta may be used in breeding programs to improve new modern cultivars with high root and shoot traits.
Show more [+] Less [-]Fatty Acid and Sterol Compositions of Hatay Olive Oils
2017
Dilsat Bozdogan Konuskan
In this study, sterol and fatty acid compositions with the other quality criteria (free fatty acids, peroxide value, total chlorophyll and carotenoid content) of olive oil samples obtained from Halhalı, Gemlik and Sarı Hasebi varieties through two phase mechanical method (crushing, kneading and centrifuge) was determined. Oleic, palmitic, linoleic, stearic, palmitoleic, linolenic and arachidic acids were the determined as the main fatty acids in olive oil samples. It was determined that oleic acid contents of oil samples ranged between 66.25-76.14% and Sarı Hasebi had the highest oleic acid content. Sterol and fatty acid compositions of olive oil samples showed significantly statistical differences according to varieties. It was determined that the total sterol contents of oils ranged between 1025 and 1686 mg/kg and varieties with the highest and lowest total sterol content were Gemlik and Sarı Hasebi. Apparent β-sitosterol contents (β-sitosterol, Δ-5-avenasterol, Δ-5-24-stigmastadienol, klerosterol, sitostanol) were between 92.96 and 94.63%. Varieties with the highest and lowest apparent β-sitosterol contents were oils which belong to Halhalı and Sarı Hasebi varieties respectively. β -sitosterol (83.08-88.21%), Δ-5-avenasterol (4.82-6.97%) and campesterol (2.28-3.43%) were identified as the main sterol components. Erythrodiol + uvaol contents of olive oils varied between 2.28 and 3.43% and these values were within the limits established by Turkish Food Codex.
Show more [+] Less [-]The Relationship Between Sweeteners and Metabolic Diseases
2017
Nildem Kızılaslan
Sweeteners that sugar substitute is a food additive that provides a sweet taste like that of sugar while containing significantly less food energy. Some sugar substitutes are produced by nature, and others produced synthetically. Sweeteners can be classified as sugar alcohols (polyols), artificial sweeteners and natural sweeteners. The need to avoid the adverse effects of sugar cannot stop feeling the taste on humans has resulted in intense use of sweeteners nowadays. There are positive and negative opinions about the effects of their use on human health. The general tendency of food producers is to reduce the amount of sugar in the groats due to negative effects in parallel. Food sweeteners are intended for a large group of consumers, and one of the main points of the sector operators is to provide healthier, natural, nutritional and low calorie sweeteners to these consumers. In this study, we evaluated the sweeteners which are most commonly used. Many studies have been done to demonstrate the safety of sweeteners. There are also studies showing that there may be side effects even if there are few. Some sweeteners have been associated with a number of symptoms. There is a need for comparative long-term work in this area.
Show more [+] Less [-]Effects of Salinity Stress on Emergence and Seedling Growth Parameters of Some Maize Genotypes (Zea mays L.)
2017
Ömer Konuşkan | Hüseyin Gözübenli | İbrahim Atiş | Mehmet Atak
Maize is a very important cereal all over the world and generally cultivated in irrigated agricultural areas. Salinity affects adversely maize productivity in these areas. Maize is known as a salt sensitive species. The salt tolerance level identification in the large genetic resources and breeding populations is an important research topic for solving the salinity problem. The effects of NaCl stress on germination and seedling growth of fifteen maize genotypes were investigated in this study. Electrical conductivity (EC) values of the NaCl were settled 0 (distilled water), 4, 6, 8, 10 and 12 dS m-1. This investigation was performed as factorial arrangement of completely randomized design with four replications. Analysis of variance (ANOVA) showed that genotypes, salinity levels and interaction between genotype x salinity were significant for all the investigated treatments emergence index (EI), root dry weight (RDW), shoot dry weight (SDW), root length (RL), shoot length (SL), and salt tolerance indexes (STI). Results also revealed that the highest reduction in emergence index (59.7%) were obtained in the highest level (12 dS m-1) of salinity. The results further revealed that the cultivars named DKC 6589, PR31G98, and PR31A34 were the most tolerant genotypes than the others under NaCI stress.
Show more [+] Less [-]Determination of Phenological Properties and Effective Heat Summation Requirements of Some Apples Varieties in Ankara (Kalecik) Conditions
2017
Hülya Ünver
In this study, the phenological characteristics of some apple varieties grown in the Kalecik district of Ankara province, the number of days between effective heat summation requirements, and phenological phases were determined. The research was conducted on Royal Gala, Granny Smith, Jersey Mac, Spur Golden and Red Chief apple varieties. The threshold temperature of +10°C was accepted as the temperature value for the determination of the heat summation requirements of the varieties. The effective heat summation above +10°C during the growing season of varieties was determined as 819.1-1986.3°C days in 2015 and 865.1-2031.2°C days in 2016. The number of days from full bloom to harvest was 85-75 days in 2015 and 132-219 days in 2016.
Show more [+] Less [-]Differential Effects of a Vermicompost Fertilizer on Emergence and Seedling Growth of Tomato Plants
2017
Yurdagül Şimşek Erşahin | Ali Ece | Ebru Karnez
Vermicompost products have gained a great importance in plant nutrition over the years. They are reported to have plant growth promoting effects both in horticulture and field crops. The nutritional value and chemical properties of vermicomposts highly depend on the feedstock used in their production. The aim of this study was to evaluate vermicompost manure, derived from the mixture of cattle manure and kitchen scraps, on seed germination and growth of tomato seedlings (Lycopersicon lycopersicum Mill.). Four solid vermicompost amendment rates of 0, 10, 20, and 30% were applied in plastic trays. Vermicompost application delayed and reduced seed emergence in all application rates, while in general, vermicompost substitution promoted growth tomato seedlings up to 20% of application rate. The results showed that vermicompost substitutions greater than 20% had adverse effects on seedling emergence and seedling growth parameters, which was attributed to high EC of vermicompost induced by cattle manure. Results suggest that both physical and chemical properties of the feedstock used for vermicompost production should be taken into consideration in order to sustain high vermicompost quality to ensure targeted plant growth for horticultural and agricultural purposes.
Show more [+] Less [-]The Effects of Different Storage Conditions on the Radiometric and Element Content of the Taşköprü Garlic (Allium sativum L.)
2017
Aslı Kurnaz | Nezahat Turfan
Garlic is very rich plant in terms of carbohydrates, enzymes, sulphurous and phenolic compounds, proteins and vitamins. At the same time, natural and artificial radionuclides and heavy metals can be found in the garlic depending on the structure of the cultivated soil. Garlic, used as medicine in the treatment of most diseases, is also consumed as raw, powder, mash and volatile garlic oil. In this study, the effects of different storage conditions on the element and radionuclide content of the garlic were determined. The results obtained from this study were compared with the limit values of international institutions. As a result, the radionuclide concentrations and element contents of Taşköprü Garlic will not pose any threat to human health even if the garlic is exposed to different storage conditions.
Show more [+] Less [-]Asetolaktat Sentez İnhibitörü Herbisitlere Dayanıklı ve Duyarlı Kokarot (Bifora radians M. Bieb.) Popülasyonlarının Morfolojik ve Biyolojik Çeşitliliğinin Belirlenmesi
2017
Emine Kaya Altop | Hüsrev Mennan
Bifora radians M. Bieb. Dünya genelinde çok geniş yayılım alanı bulan Asya’ya özgü tek yıllık bir yabancı ottur. Geniş bir ekolojik toleransının ve yüksek rekabet yeteneğinin olması, B. radians’ı buğday yetiştiriciliği yapılan alanlarda sorun haline getirmiştir. Herbisitlere dayanıklılığın, popülasyon dinamiğinin bilinme gerekliliği ve demografik verilere duyulan ihtiyaçtan dolayı bitkilerdeki varyasyon çalışmaları önem arz etmektedir. Asetolaktat sentez inhibitörü herbisitlere dayanıklı 29 ve duyarlı 22 olmak üzere 51 lokasyondan toplanan B. radians popülasyonları morfolojik ve biyolojik farklılıklarına göre karşılaştırılmıştır. Sonuçlara göre parametre verileri dayanıklı ve hassas popülasyonlar arasında belirgin farklılığı göstermiş ve bu çeşitlilik benzer lokasyonlar için de geçerli olmuştur. B. radians genotipleri arasındaki morfolojik ve biyolojik çeşitlilik; coğrafi lokasyonlar, farklı aktif maddeli ve farklı etki tarzlarına sahip herbisit uygulamaları, yetiştirilen ürünler ve uygulanan farklı tarımsal uygulamalar nedeniyle meydana gelebilmektedir. Yabancı ot popülasyonları arasındaki farklılık biyolojik ve kimyasal mücadele çalışmalarını etkileyebilmektedir.
Show more [+] Less [-]Assessment of Irrigation Practices of Farmers Receiving Water from Open-Canal and Piped Irrigation Networks
2017
Nihan Körpe Aslan | Murat Tekiner
Open-canal irrigation networks have been transformed into closed-piped networks. Drip irrigation is the most common method used irrigations in Çanakkale province. The aim of study is to assess irrigation practices of farmers using different irrigation networks. There were not significant differences in irrigation practices of the farmers receiving water from both networks, but all were making mistakes and unconscious in their practices. An optimum schedule was also prepared to compare with farmer practices. While a farmer growing tomato in Kumkale village with piped-network was expected to apply 531 mm irrigation water as seasonal total, present farmer applied 1414 mm water; while a farmer growing tomato in Mahmudiye village with open-canal network was expected to apply a total of 457 mm, present farmer applied 492 mm irrigation water; while a farmer growing maize in Kumkale village with piped-network was expected to apply 512 mm, the present farmer applied 975 mm; finally a farmer growing maize in Mahmudiye village with open-canal network was expected to apply 379 mm water, the present farmer applied 228 mm irrigation water throughout the entire growing season. Irrigation water efficiency (IWUE) values indicating the effects of irrigation water quantities on yields were calculated as 2.5 kg/da/mm for tomato culture in Kumkale; 12.3 kg/da/mm for tomato culture in Mahmudiye; 0.7 kg/da/mm for maize culture in Kumkale and 8,7 kg/da/mm for maize culture in Mahmudiye village. Relative irrigation supply (RIS) values indicating how efficiently the precipitations were used were calculated as 2.98 for tomato culture in Kumkale; 1.41 for tomato culture in Mahmudiye; 2.40 for maize culture in Kumkale and 0.85 for maize culture in Mahmudiye village.
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