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Detection of foot and mouth disease virus shedding in milk of apparently healthy buffaloes and cattle of Punjab, Pakistan
2019
Zeeshan Nawaz(Government College University, Faisalabad (Pakistan). Department of Microbiology) E-mail:zeeshannawaz@gcuf.edu.pk | Abu Baker Siddique(Government College University, Faisalabad (Pakistan). Department of Microbiology) | Muhammad Asif Zahoor(Government College University, Faisalabad (Pakistan). Department of Microbiology) | Bilal Aslam(Government College University, Faisalabad (Pakistan). Department of Microbiology) | Muhammad Kashif Zahoor(Government College University, Faisalabad (Pakistan). Department of Zoology) | Shahid Ali(Government College University, Faisalabad (Pakistan). Department of Microbiology) | Sultan Ali(University of Agriculture, Faisalabad (Pakistan). Institute of Microbiology)
Foot and mouth disease is among the top listed livestock diseases causing severe economic losses. The aim of the present study was to determine the seroprevalence of FMD and detection of FMD virus shedding in milk of apparently healthy buffaloes and cattle of Pakistan. A total of 30 dairy farms were selected and registered in rural areas of Punjab consisting of minimum 15 animals. A total of 180 serum samples were collected and subjected to Non structural proteins (NSP) ELISA. The milk of sero-positive animals were collected and detected for the presence of FMD virus using reverse transcriptase PCR (RT-PCR). The results of current study showed overall seroprevalence 71.66 percent (129/180) with 65.38 percent in buffaloes and 76.47 percent in cattle. The FMD virus was detected in 24.03 percent (31/129) of sero positive samples. Among the FMD virus positive samples 65.51 percent belongs to serotype O and 35.48 percent belongs to Asia I, while none of the other serotypes were detected. The detection of FMD virus from the milk of apparently healthy buffaloes and cattle is an alarming situation and it may be considered as a potential role in the transmission of FMD.
Show more [+] Less [-]Interferon-gamma messenger RNA level in peripheral blood mononuclear cells of naive bovines after foot and mouth disease virus vaccination and challenge
2019
S.D. Audarya(Nanaji Deshmukh Veterinary Science University, Madhya Pradesh (India). College of Veterinary Science and Animal Husbandry. Department of Veterinary Microbiology) E-mail:asd_vet@yahoo.com | A. Sanyal(Indian Veterinary Research Institute Campus, Uttarakhand (India)) | J.K. Mohapatra(Indian Veterinary Research Institute Campus, Uttarakhand (India)) | L.K. Pandey(Indian Veterinary Research Institute Campus, Uttarakhand (India)) | R. Kumar(Indian Immunologicals Limited, Andhra Pradesh (India). Research and Development Centre. Foot-and-Mouth Disease Virus Laboratory) | M. Kankipati(Indian Immunologicals Limited, Andhra Pradesh (India). Research and Development Centre. Foot-and-Mouth Disease Virus Laboratory) | Y. Shanmugam(Indian Immunologicals Limited, Andhra Pradesh (India). Research and Development Centre. Foot-and-Mouth Disease Virus Laboratory) | A. Jangham(Indian Immunologicals Limited, Andhra Pradesh (India). Research and Development Centre. Foot-and-Mouth Disease Virus Laboratory) | N.B. Singanallur(Australian Animal Health Laboratory, Victoria (Australia). CSIRO-Health and Biosecurity) | B. Pattnaik(Indian Veterinary Research Institute Campus, Uttarakhand (India))
The study evaluated the antibody response and interferon-gamma (IFN-gamma) messenger ribonucleic acid (mRNA) expression levels in naive bovine calves from a Foot-and-Mouth Disease virus (FMDV) potency test. Peripheral blood mononuclear cells (PBMCs) were separated from blood samples and total RNA was extracted. Two step quantitative (Real time-polymerase chain reaction (RT-PCR) method was used for measurement of IFN-gamma specific mRNA. Plasma samples were screened to detect anti-non structural proteins (NSP) antibodies for FMDV in 3AB NSP antibody enzyme linked immunosorbent assay (ELISA). Plasma samples were found free for anti- NSP antibodies for FMDV in vaccinated bovine calves as evaluated in 3AB NSP antibody ELISA test. Increase in mRNA expression level of IFN-gamma after 15 days post vaccination (DPV) was found unequivocally in all five vaccinated calves (more than two-fold increase in 4 out of 5 calves). Liquid phase blocking ELISA (LPBE) test results also revealed highest humoral antibody response on 15 DPV. Monovalent FMDV vaccination elicited highest antibody titre on 15 DPV in correlation with elevated IFN-gamma mRNA level. Humoral and cellular immunity both play important role in protection against FMDV. The study will help in further understanding the role played by both the humoral and cellular immune response against FMDV.
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