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Preliminary report on cryopreservation of Thai swamp buffalo embryos : Manual and automatic methods
1989
Techakumphu, M. | Lohachit, C | Chantaraprateep, P. (Chulalogkron Univ., Bangkok (Thailand). Faculty of Veterinary Science Dept. of Obstetrics Gynaecology and Reproduction)
Total of nine swamp buffalo embryos at the stages of two-cell embryo, 16-cell embryo, compacted morula, blastocyst and hatched blastocyst were frozen to -196 deg. Celsius by manual and automatic methods. The embryos were cooled slowly from room temperature to -7 deg. Celsius at the rate of 1.0 deg. Celsius/min from -7 deg. Celsius to -15 deg. Celsius or -30 deg. Celsius at the rate 0.3 deg. Celsius/min before plunging into liquid nitrogen. The post-thawed morphology showed undamaged embryos (grade A) could be obtained after both manual and automatic freezing, and also partially damaged (grade B) and totally damaged embryos (grade C). The percentage of each group was 22.2 % (2/9), and 55.6 % (5/9), respectively. This was the first report on cryopreservation of swamp buffalo embryos. Further studies on biabiliity after transfer should follow
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