This study was conducted to analyze the consequence of prill fat and rumen protected choline (RPC) supplementation on performance of lactating Murrah buffaloes. Murrah buffaloes (n=18) were distributed into three groups (Control, T1 and T2) having six buffaloes in each, based on milk yield, fat per cent and stage of lactation. During the experimental period of 90 days, buffaloes in all the three groups were fed same basal diet, containing green fodder (Berseem), wheat straw and concentrate mixture to meet their requirements for maintenance and milk production. Buffaloes in Group T1 were daily supplemented with prill fat 2.5 percent of total DMI whereas; buffaloes in Group T2 were daily supplemented with 54 g rumen protected choline along with same quantity of prill fat fed as in Group T1. In contrast to the control group, overall milk yield (kg) of lactating Murrah buffaloes was significantly (P LT 0.01) increased by 0.99 and 1.78 kg in Group T1 and T2. The average 4 percent FCM (kg/d), ECM (kg/d) and fat yield (kg/d) was significantly (P LT 0.01) higher in Group T2 followed by Group T1 and Group control. There was no significant difference in milk composition of buffaloes except milk fat (percent) and total solids (percent) which were improved significantly (P LT 0.01) in supplemented groups. It was observed that prill fat supplementation in the ration helped to make better performance of early lactating Murrah buffaloes by enhanced milk yield and fat percent which can be further increased by supplementing the ration with rumen protected choline. Overall milk yield (kg) of lactating Murrah buffaloes was significantly (p LT 0.01) increased by 0.99 and 1.78 kg in T1 and T2 groups, as compared to the control group. The average 4 percent FCM (kg/d), ECM (kg/d) and fat yield (kg/d) was significantly (p LT 0.01) higher in T2 group followed by T1 and control group. There was no significant difference in milk composition of buffaloes except milk fat (percent) and total solids (percent) which were improved significantly (p LT 0.01) in supplemented groups. It was concluded that prill fat supplementation in the ration helped in improving performance of early lactating Murrah buffaloes by increasing milk yield and fat percent which can be further enhanced by supplementing the ration with rumen protected choline.