Habitat analysis, include biophysics environmental conditions of swamp buffalo (Bubalus bubalis) has been conducted in June until December 2017 in Rambutan (Banyuasin) and Ogan Ilir (Indralaya) district, South Sumatra and the laboratory of Animal Physiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The purpose of research is to know swamp buffalo habitat characteristics at the district of Rambutan (Banyuasin) and Indralaya (Ogan Ilir) by physical, chemical, soil and vegetation conditions in the habitat of buffalo. The sampling method of buffalo was used purposive sampling method wheareas vegetation sampling using the quadrant method or plot. Data analysis techniques used are in qualitative and quantitative. The data is then presented in the form of tables and diagrams and narratives to interpret the data. Based on identification and field observations of vegetation conditions, Rambutan vegetation was consist of 15 species and Indralaya vegetation was consist of 9 species for swamp buffalo food. Temperature inside and outside of cage in Indralaya is 22.3 to 31.3 deg C while temperature in Rambutan is 23.2 to 32.8 deg C. Potential of hydrogen soil is 5.10 to 5.60 and always inundated by water. It can be concluded that the form of lowland biophysics environmental. Conditions of swamp buffalo dominated by grasses belonging to the family of Cyperaceae, Graminae, Fabaceae and Poaceae as the main source of food, while the state of the temperature is very supportive for buffalo habitat.