This research examined the effects of storage temperature and storage time on the somatic cell count (SCC) of milk from Anatolian buffaloes, which was measured with the DeLaval cell counter (DCC). Storage temperature and time are among the different factors that potentially affect the SCC of Anatolian buffalo milk. In this context, 20 milk samples were collected from Anatolian Buffaloes and analyzed. The milk samples were divided into two groups according to their measured level of SCC. These two groups were the low score (LT= 3.16 cell/ml) group and the high score (GT 3.16 cell/ml) group. The mean logSCC values of the low score and the high score groups were determined as 2.27+-0.045 and 4.06+-0.019 cells/ml, respectively. In this research, the effects of storage temperature (4 deg C, 21 deg C) and storage time (fresh milk, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and 24 h) on logSCC were determined to be statistically significant (P LT 0.01). Thus, increases in storage temperature and storage time were associated with an increase in the logSCC of the milk samples. In this study, the effects of storage temperature (4 deg C, 21 deg C) and storage time (fresh milk, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12 and 24 hours) on logSCC were determined to be statistically significant (P LT 0.01). Thus, increases in storage temperature and storage time were associated with an increase in the logSCC of the milk samples.

The present study was conducted to investigate the normal somatic cell count (SCC), associated factors in hygienic management and to define subclinical mastitis in buffaloes in Puducherry. Samples were collected from 50 clinically normal buffaloes stationed at different farms of Puducherry region. Somatic cell count was measured for all the milk samples using Newman-Lampert staining technique. The upper limit of SCC was determined as GT= 2, 00,000/ml of milk based on the mean +- 2SD of a total SCC. California mastitis test (CMT) was conducted for all the animals. In the present study subclinical mastitis was diagnosed on the basis of samples with SCCs GT= 2, 00,000/ml. In the present study 96 percent of the milk samples were negative for California mastitis test (CMT). The SCC of CMT positive quarter was significantly higher (P LT 0.01) than CMT negative quarters. Subclinical mastitis was found only in three buffaloes in the present study. Somatic cell counts were significantly higher in the buffaloes with subclinical mastitis. Regarding the hygiene, all the farms had poor management and improper housing. Proper education on hygienic management is need of the hour for buffalo owners of Puducherry region.

The present study was designed to correlate the milk pH, somatic cell count (SCC), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP) activities in buffalo milk with the udder health status. A total of 60 (Sixty) buffaloes were randomly selected irrespective of their age, breed, feeding practice, stage and season of lactation from the herd belonging to Purnadhadi buffalo unit, PGIVAS, Teaching Veterinary Clinical complex, PGIVAS, Veterinary Polyclinic, Akola and also buffaloes of farmers in and around Akola. After thorough clinical examination of each udder, about 30 ml of milk was collected in sterilized, clean, dry plastic bottles and after careful screening by CMT, categorized in normal, sub- clinical (1+), sub- clinical (2+), sub- clinical (3+) and clinical group containing 12 animals in each group. The pH was measured using a digital pH meter (E.I. Model 101E) and SCC was calculated in freshly collected milk. LDH and ALP activities were measured by spectrophotometric method. The milk pH, SCC, LDH and ALP activities were found proportional to the severity of the udder infection as detected by the CMT reactions.