Study was undertaken to investigate the fertility of frozen semen from three Nili-Ravi buffalo bulls using three different extenders. Semen was extended in milk-yolk-glycerol, lactose-yolk-glycerol and fructose-lactose-yolk-glycerol, kept in 0.5 ml straws and frozen in liquid nitrogen. Only normal cyclic buffaloes were inseminated with the post-thaw semen. Fertility was based on pregnancy test through rectal palpation at 45 to 50 days post insemination. Milk-yolk-glycerol extender showed the best fertility result (49.82 percent) among all the extenders (45.68 percent for lactose-yolk-glycerol and 39.01 % for fructose-lactose-yolk-glycerol)

In the present investigation, plasma levels and pharmacokinetics of cephaloridine were investigated in buffalo calves following intramuscular administration (10 mg/kg) 6 h after its iv injection (10 mg/kg). The appreciable plasma concentration of 2.78+-0.28 ug ml-1 was recorded at 1 min and the peak plasma level (31.6+-1.50 ug ml-1) was measured at 30 min. The absorption half-life and elimination half-life were 0.037+-0.011 and 2.24+-0.30 h, respectively. In general the disposition behaviour of cephaloridine when given by repeated administration did not vary significantly from its single intramuscular dose.

Dry matter requirement was estimated on 24 growing Indian buffalo calves fed concentrate and wheat straw to supply two levels (75 and 100 %) of protein and three levels (90, 100 and 110 %) of energy as per NRC (1976) for 500 g daily gain in a 2x3 factorial design. The dry matter intake (DMI) recorded at about 100 kg and 250 kg mean body weight was used for deriving multiple regression using DMI (Y) an independent variable and body weight or metabolic body weight (X1) and average daily gain (X2) as dependent variables. The prediction equations were: 1) Y= -0.9956+0.1036 X1 +0.0015 X2, 2. Y= -0.0489+0.02344 X1 +0.0015 X2, 3) Y= -4.9946+0.1315 X1 +0.002665 X2 and 4) Y= -1.782+0.019 X1 +0.00355 X2. There was positive correlation between DMI with body weight or metabolic body weight and ADG for all the 4 equations and R2 was 0.32; 0.32; 0.66 and 0.56 respectively. In spite of great differences in correlation coefficients, equations 2, 3 and 4 were found to be useful for predicting dry matter requirement from 100 to 400 kg body weight and from zero to 750 g ADG. However, equation 1 may be useful for 100 to 200 kg body weight

Study was carried out to find out the association between physical traits and production characteristics in 25 Surti buffaloes. Out of the six traits studied only height at withers (r=-0.446) and distance between pin-bones (r=+0.455) were found to be significantly related the milk yield but their regression coefficient was of little practical value. None of the other characters studied showed any association with milk yield or fat percent in Surti buffaloes.

Infestation by leeches (Hirudinia sp.) was observed in Murrah buffaloes of Song Be, Vietnam, mostly during the humid-hot season. Percentage incidence of attachment was about 31.14, 22.75, 15.75, 10.78, 7.19, 5.39, 4.19 and 2.99 in the region of udder and teats, inguinal region, vulva and vagina, nasal cavity, lips and chin, base of the ears, naval region and neck and brisket, respectively. After blood sucking for different periods, mean body weight of leeches was 338.12, 291.85, 245.51, 204.17, 139.53, 118.82, 103.80 and 98.73 g in the corresponding order of attachments. The body weight of partly and fully fed leeches ranged from lowest 12 g detached from neck and brisket region to highest 517 g fallen after full feeding from the udder and teats.

Total of 1189 records of Egyptian buffaloes at Mehallet Mousa farm belonging to Ministry of Agriculture were used to study the influence of year and season of calving and parity on each of days open, calving interval and dry period were studied. The estimate of the phenotypic and genetic parameters of these traits were also investigated. Least squares analysis of variance showed a highly significant effect of year and season of calving and parity on all traits (P