ABSTRACT Biomass produced in agricultural areas stores energy that can be used, contributing to regional development. Among the widely cultivated agricultural species is oats, destined for the production of not only grains and forage, but also biomass. The objective of this study was to characterize oat biomass in terms of the potential for energy generation considering the genetic and cultivation environment variability. Four field experiments were conducted in the state of Paraná and one in the state of São Paulo, Brazil, with black oat (Avena strigosa) and white oat (Avena sativa) cultivars. At the milky grain stage, plants were collected to quantify the production of shoot biomass and its qualitative variables for energy production and energy potential. Biomass yield varied between cultivars and cultivation sites. The mean higher calorific value was 17.9 MJ Kg-1, varying more between cultivation sites than between cultivars, being inversely proportional to the ash content. The contents of carbon, fixed carbon, volatile materials and nitrogen in the biomass did not vary between oat cultivars. The power generation potential varied widely between cultivars and cultivation sites, from 1557 to 3091 KWh ha-1, influenced mainly by the biomass yield, which overlaps the effects of the variations found in biomass quality. We concluded that oats are a species with high potential for use as an energy product, and the selection of the most productive cultivars regionally is crucial.