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GENETIC DIVERSITY AMONG AND WITHIN BRAVE BEAN (Capparis flexuosa L.) POPULATIONS ASSESSED USING RAPD MARKERS Full text
2019
Jorge Xavier de Almeida | Mailson Monteiro do Rêgo | Elizanilda Ramalho do Rêgo | Ana Paula Gomes da Silva
GENETIC DIVERSITY AMONG AND WITHIN BRAVE BEAN (Capparis flexuosa L.) POPULATIONS ASSESSED USING RAPD MARKERS Full text
2019
Jorge Xavier de Almeida | Mailson Monteiro do Rêgo | Elizanilda Ramalho do Rêgo | Ana Paula Gomes da Silva
Brave bean (Capparis flexuosa L.) is a Caatinga species that is used as forage, mainly during the dry season when some plant species lose their leaves. The aim of this study was to assess genetic diversity within and among brave bean populations using Random Amplified Polymorphic DNA (RAPD) markers. Brave bean leaves were collected from 30 accessions in the following municipalities of Paraíba state, Brazil: Barra de Santa Rosa (BSR), Cuité (C), São João do Cariri (SJC), Damião (D), Baraúna (B), and Picuí (P). DNA extraction followed the standard methodology of CTAB with modifications. RAPD analyses were carried out using 18 primers, and polymorphism of the amplified DNA fragments was visualized using agarose gel electrophoresis. Data were used to calculate Jaccard Similarity Coefficient values, which were then used to group samples with the Unweighted Pair Group Method with Arithmetic Mean. Cophenetic Correlation Coefficient, Stress, and Distortion Coefficient values were also calculated from these analyses. Band polymorphism was generated with 14 primers, but the sampled populations showed low numbers of polymorphic loci (27 in BSR, 18 in C, 7 in SJC, 9 in D, and 0 in B and P). The highest polymorphic information content was found in samples from the BSR (9 groups), C (22 groups), SJC (7 groups), and D (6 groups) municipalities. In the interpopulation analysis, 34 groups were formed, the matrices of which showed high cophenetic correlations (0.95 to 0.98), but low stress (12.9 to 17.45%) and distortion (3.05%). Therefore, results showed that there was genetic variability both among and within brave bean populations.
Show more [+] Less [-]GENETIC DIVERSITY AMONG AND WITHIN BRAVE BEAN (Capparis flexuosa L.) POPULATIONS ASSESSED USING RAPD MARKERS Full text
2019
Almeida Neto, Jorge Xavier de | Rêgo, Mailson Monteiro do | Rêgo, Elizanilda Ramalho do | Silva, Ana Paula Gomes da
RESUMO O feijão-bravo (Capparis flexuosa) é uma espécie nativa da Caatinga e é utilizada como forrageira, principalmente na estação seca onde parte das espécies vegetais perdem suas folhas. Objetivou-se estudar a diversidade genética dentro e entre populações de feijão-bravo usando marcadores RAPD. O estudo foi realizado no Laboratório de Biotecnologia Vegetal do CCA/UFPB. Foram coletadas folhas de 30 acessos de feijão-bravo/população nos seguintes municípios do estado da Paraíba: Barra de Santa Rosa (BSR), Cuité (C), São João do Cariri (SJC), Damião (D), Baraúna (B) e Picuí (P). A extração de DNA seguiu a metodologia de utilização do CTAB com modificações. Análises de RAPD foram realizadas usando 18 primers e o polimorfismo de fragmentos de DNA amplificados foi visualizado em gel de agarose após eletroforese. Os dados foram submetidos ao Coeficiente de Similaridade Jaccard, agrupados seguindo a UPGMA e calculou-se o Coeficiente de Correlação Cofenética, Estresse e Distorção. 14 primers geraram polimorfismo de banda, no entanto, as populações apresentaram baixo número de locos polimórficos (27 em BSR, 18 em C, 7 em SJC, 9 em D, 0 em B e P) sendo o maior conteúdo de informação polimórfica foi encontrado nos municípios de BSR (9 grupos), C (22 grupos), São João do Cariri (7 grupos) e D (6 grupos). Formou-se 34 grupos na análise interpopulacional. As matrizes formadas apresentaram altos índices de correlação cofenética (0,95 a 0,98), baixo estresse (12,9 a 17,45%) e distorção (3,05%). Portanto, há variabilidade genética entre e dentro de populações de feijão-bravo. | ABSTRACT Brave bean (Capparis flexuosa L.) is a Caatinga species that is used as forage, mainly during the dry season when some plant species lose their leaves. The aim of this study was to assess genetic diversity within and among brave bean populations using Random Amplified Polymorphic DNA (RAPD) markers. Brave bean leaves were collected from 30 accessions in the following municipalities of Paraíba state, Brazil: Barra de Santa Rosa (BSR), Cuité (C), São João do Cariri (SJC), Damião (D), Baraúna (B), and Picuí (P). DNA extraction followed the standard methodology of CTAB with modifications. RAPD analyses were carried out using 18 primers, and polymorphism of the amplified DNA fragments was visualized using agarose gel electrophoresis. Data were used to calculate Jaccard Similarity Coefficient values, which were then used to group samples with the Unweighted Pair Group Method with Arithmetic Mean. Cophenetic Correlation Coefficient, Stress, and Distortion Coefficient values were also calculated from these analyses. Band polymorphism was generated with 14 primers, but the sampled populations showed low numbers of polymorphic loci (27 in BSR, 18 in C, 7 in SJC, 9 in D, and 0 in B and P). The highest polymorphic information content was found in samples from the BSR (9 groups), C (22 groups), SJC (7 groups), and D (6 groups) municipalities. In the interpopulation analysis, 34 groups were formed, the matrices of which showed high cophenetic correlations (0.95 to 0.98), but low stress (12.9 to 17.45%) and distortion (3.05%). Therefore, results showed that there was genetic variability both among and within brave bean populations.
Show more [+] Less [-]STATISTICAL MULTIVARIATE ANALYSIS APPLIED TO ENVIRONMENTAL CHARACTERIZATION OF SOIL IN SEMIARID REGION Full text
2019
Antônio Italcy de Oliveira | Luiz Alberto Ribeiro Mendonça | Sávio de Brito Fontenele | Adriana Oliveira Araújo | Maria Gorethe de Sousa Lima Brito
Soil is a dynamic and complex system that requires a considerable number of samples for analysis and research purposes. Using multivariate statistical methods, favorable conditions can be created by analyzing the samples, i.e., structural reduction and simplification of the data. The objective of this study was to use multivariate statistical analysis, including factorial analysis (FA) and hierarchical groupings, for the environmental characterization of soils in semiarid regions, considering anthropic (land use and occupation) and topographic aspects (altitude, moisture, granulometry, PR, and organic-matter content). As a case study, the São José Hydrographic Microbasin, which is located in the Cariri region of Ceará, was considered. An FA was performed using the principal component method, with normalized varimax rotation. In hierarchical grouping analysis, the “farthest neighbor” method was used as the hierarchical criterion for grouping, with the measure of dissimilarity given by the “square Euclidean distance.” The FA indicated that two factors explain 75.76% of the total data variance. In the analysis of hierarchical groupings, the samples were agglomerated in three groups with similar characteristics: one with samples collected in an area of the preserved forest and two with samples collected in areas with more anthropized soils. This indicates that the statistical tool used showed sensitivity to distinguish the most conserved soils and soils with different levels of anthropization.
Show more [+] Less [-]POST-HARVEST QUALITY OF OZONATED MACAUBA FRUITS FOR BIODIESEL PRODUCTION Full text
2019
Gutierres Nelson Silva | José Antônio Saraiva Grossi | Marcela Silva Carvalho | Samuel de Melo Goulart | Lêda Rita D'Antonino Faroni
POST-HARVEST QUALITY OF OZONATED MACAUBA FRUITS FOR BIODIESEL PRODUCTION Full text
2019
Gutierres Nelson Silva | José Antônio Saraiva Grossi | Marcela Silva Carvalho | Samuel de Melo Goulart | Lêda Rita D'Antonino Faroni
The presence of microorganisms, especially during storage, can lead to the rapid deterioration of macauba fruits (Acrocomia aculeata) and reduce the quality of extracted oil for biodiesel production. An alternative to ensure the quality of the oil is the use of ozone gas, a oxidizing agent that has high antimicrobial and sanitizing power. The objective of this study was to evaluate the influence of high concentrations of ozone gas on macaúba fruit and its effect on the quality of the oil throughout storage for the production of biodiesel. Ozonation was performed 20 days after harvest using fruits with and without the epicarp. Ozone was applied at a concentration of 18.0 mg L-1 and a flow of 1.5 L min-1 for 10 h. After ozonation, the fruits were stored for different periods, and the physical-chemical parameters of the oil were evaluated. The ozonation of fruits without the epicarp visually reduced microorganism attack. In general, the ozonation process of macauba fruits maintained the visual characteristics of the fruits and the physical-chemical characteristics of the oil for biodiesel production compared to untreated fruits. The average acidity values of the oil were lower in the treatments with the ozone gas. Removal of the epicarp tends to facilitate the ozonation process, potentially by improving gas penetration into the fruit.
Show more [+] Less [-]POST-HARVEST QUALITY OF OZONATED MACAUBA FRUITS FOR BIODIESEL PRODUCTION Full text
2019
Silva, Gutierres Nelson | Grossi, José Antônio Saraiva | Carvalho, Marcela Silva | Goulart, Samuel de Melo | Faroni, Lêda Rita D'Antonino
RESUMO A presença de micro-organismo, especialmente durante o armazenamento, pode levar à deterioração rápida dos frutos de macaúba (Acrocomia aculeata) e reduzir a qualidade do óleo extraído para a produção de biodiesel. Uma alternativa para garantir a qualidade do óleo é o uso de gás ozônio, um poderoso agente oxidante que possui alto poder antimicrobiano e sanitizante. O objetivo deste estudo foi avaliar a influência do ozônio gasoso em alta concentração (18,0 mg L-1) em frutos de macaúba (com e sem epicarpo) e seu efeito sobre a qualidade do óleo durante todo o armazenamento para a produção de biodiesel. A ozonização foi realizada 20 dias após a colheita, utilizando frutas com e sem epicarpo. O ozônio foi aplicado na concentração de 18,0 mg L-1 e fluxo de 1,5 L min-1 durante 10 h. Após a ozonização, os frutos foram armazenados por diferentes períodos e foram avaliados os parâmetros físico-químicos do óleo. A ozonização de frutos sem epicarpo reduziu visualmente o ataque de micro-organismos. Em geral, o processo de ozonização de frutos de macauba manteve as características visuais dos frutos e físico-químicas do óleo para produção de biodiesel em relação ao controle não tratado. Os valores médios de acidez do óleo foram menores nos tratamentos com o gás ozônio. A remoção do epicarpo tende a facilitar o processo de ozonização, já que a retirada pode facilitar a penetração do gás no fruto. | ABSTRACT The presence of microorganisms, especially during storage, can lead to the rapid deterioration of macauba fruits (Acrocomia aculeata) and reduce the quality of extracted oil for biodiesel production. An alternative to ensure the quality of the oil is the use of ozone gas, a oxidizing agent that has high antimicrobial and sanitizing power. The objective of this study was to evaluate the influence of high concentrations of ozone gas on macaúba fruit and its effect on the quality of the oil throughout storage for the production of biodiesel. Ozonation was performed 20 days after harvest using fruits with and without the epicarp. Ozone was applied at a concentration of 18.0 mg L-1 and a flow of 1.5 L min-1 for 10 h. After ozonation, the fruits were stored for different periods, and the physical-chemical parameters of the oil were evaluated. The ozonation of fruits without the epicarp visually reduced microorganism attack. In general, the ozonation process of macauba fruits maintained the visual characteristics of the fruits and the physical-chemical characteristics of the oil for biodiesel production compared to untreated fruits. The average acidity values of the oil were lower in the treatments with the ozone gas. Removal of the epicarp tends to facilitate the ozonation process, potentially by improving gas penetration into the fruit.
Show more [+] Less [-]PRODUCTIVE VIABILITY AND PROFITABILITY OF CARROT-COWPEA INTERCROPPING USING DIFFERENT AMOUNTS OF Calotropis procera Full text
2019
Francisco Bezerra Neto | Maiele Leandro da Silva | Jailma Suerda Silva de Lima | Aurélio Paes Barros Júnior | Italo Nunes Silva | Aridênia Peixoto Chaves
PRODUCTIVE VIABILITY AND PROFITABILITY OF CARROT-COWPEA INTERCROPPING USING DIFFERENT AMOUNTS OF Calotropis procera Full text
2019
Francisco Bezerra Neto | Maiele Leandro da Silva | Jailma Suerda Silva de Lima | Aurélio Paes Barros Júnior | Italo Nunes Silva | Aridênia Peixoto Chaves
Intercropping system and the use of green manure with spontaneous species has been an alternative farming method applied to the productive sector of vegetables in the northeastern semi-arid region. The objective of this work was to determine which amount of Calotropis procera must be incorporated into the soil to provide the highest productive performance of the component crops and increase the profitability of the carrot and cowpea intercropping. The experimental design used was randomized complete blocks with five replicates. The treatments consisted of four amounts of C. procera incorporated into the soil: 10, 25, 40 and 55 t ha-1 on a dry basis. The characteristics evaluated in this intercropping system were: commercial productivity of carrot roots, yield of cowpea green grains, land equivalent ratios for component crops and for the intercropped system, score of the canonical variable of the association, and the economic indicators of gross income, net income, rate of return, and net profit margin. The maximum agronomic efficiency of the carrot x cowpea intercropping was reached at the land equivalent ratio of 1.12, using 43.39 t ha-1 of C. procera biomass incorporated in the soil, while the maximum economic efficiency of the carrot and cowpea crops association was obtained at the net income of R$ 17,856.43 ha-1, in the amount of 40.60 t ha-1 of C. procera biomass added to the soil.
Show more [+] Less [-]PRODUCTIVE VIABILITY AND PROFITABILITY OF CARROT-COWPEA INTERCROPPING USING DIFFERENT AMOUNTS OF Calotropis procera Full text
2019
Bezerra Neto, Francisco | Silva, Maiele Leandro da | Lima, Jailma Suerda Silva de | Barros Júnior, Aurélio Paes | Silva, Italo Nunes | Chaves, Aridênia Peixoto
RESUMO Sistema consorciado e uso de adubação verde com espécies espontâneas tem sido um método alternativo de cultivo aplicado ao setor produtivo de hortaliças na região semiárida nordestina. O objetivo deste trabalho foi determinar qual quantidade de Calotropis procera deve ser incorporada ao solo para proporcionar o melhor desempenho produtivo das culturas componentes e aumentar a rentabilidade do consórcio de cenoura e caupi. O delineamento experimental utilizado foi de blocos completos casualizados com cinco repetições. Os tratamentos consistiram de quatro quantidades de C. procera incorporadas ao solo: 10, 25, 40 e 55 t ha-1, em base seca. As características avaliadas neste sistema consorciado foram: produtividade comercial de raízes de cenoura, produtividade de grãos verdes de caupi, índices de uso eficiente da terra das culturas componentes e do consórcio, escore da variável canônica da associação e os indicadores econômicos renda bruta, renda líquida, taxa de retorno e margem de lucro líquido. A máxima eficiência agronômica do consórcio cenoura x caupi foi alcançada no índice de uso eficiente da terra de 1,12, utilizando-se 43,39 t ha-1 de biomassa de C. procera incorporada ao solo, enquanto a eficiência econômica máxima da associação cenoura x caupi foi obtida com a renda líquida de R$ 17.856,43 ha-1, na quantidade de 40,60 t ha-1 de biomassa de C. procera adicionada ao solo. | ABSTRACT Intercropping system and the use of green manure with spontaneous species has been an alternative farming method applied to the productive sector of vegetables in the northeastern semi-arid region. The objective of this work was to determine which amount of Calotropis procera must be incorporated into the soil to provide the highest productive performance of the component crops and increase the profitability of the carrot and cowpea intercropping. The experimental design used was randomized complete blocks with five replicates. The treatments consisted of four amounts of C. procera incorporated into the soil: 10, 25, 40 and 55 t ha-1 on a dry basis. The characteristics evaluated in this intercropping system were: commercial productivity of carrot roots, yield of cowpea green grains, land equivalent ratios for component crops and for the intercropped system, score of the canonical variable of the association, and the economic indicators of gross income, net income, rate of return, and net profit margin. The maximum agronomic efficiency of the carrot x cowpea intercropping was reached at the land equivalent ratio of 1.12, using 43.39 t ha-1 of C. procera biomass incorporated in the soil, while the maximum economic efficiency of the carrot and cowpea crops association was obtained at the net income of R$ 17,856.43 ha-1, in the amount of 40.60 t ha-1 of C. procera biomass added to the soil.
Show more [+] Less [-]SPATIALIZATION OF ELECTRICAL CONDUCTIVITY AND PHYSICAL HYDRAULIC PARAMETERS OF SOILS UNDER DIFFERENT USES IN AN ALLUVIAL VALLEY Full text
2019
Iug Lopes | Abelardo Antônio de Assunção Montenegro
SPATIALIZATION OF ELECTRICAL CONDUCTIVITY AND PHYSICAL HYDRAULIC PARAMETERS OF SOILS UNDER DIFFERENT USES IN AN ALLUVIAL VALLEY Full text
2019
Iug Lopes | Abelardo Antônio de Assunção Montenegro
Evaluating spatial variability of hydraulic properties and salinity of soils is important for an adequate agricultural management of alluvial soils, and protection of riparian vegetation. Thus, the objective of this work was to evaluate the accuracy of geophysical techniques for indirect measurements of apparent electrical conductivity (ECa), using an electromagnetic induction equipment (EM38®), and soil physical hydraulic parameters and their spatial interrelations. The study was carried out at the Advanced Research Unit of the UFRPE, in the Brígida River Basin, in Panamirim, state of Pernambuco, Brazil, in the second half of 2016. This river had a 100 m wide riparian forest strip transversely to the river bank on both sides of the river. A regular 20×10 m grid with 80 points was used to evaluate the soil hydraulic conductivity and ECa. The geostatistics showed the spatial dependence and the dependence of the soil attributes, their spatialization, and precise mapping through indirect readings. Most of the variability (86%) in soil electrical conductivity was explained by indirect readings using the EM38®. Ranges of 80 m, 380 m, and 134 m were found for soil moisture, ECa, and hydraulic conductivity, respectively, presenting strong spatial dependence. The results showed the importance of riparian forests to the maintenance of soil moisture and porosity to the improvement of soil water infiltration capacity even under severe water deficit conditions and soil subsurface layers.
Show more [+] Less [-]SPATIALIZATION OF ELECTRICAL CONDUCTIVITY AND PHYSICAL HYDRAULIC PARAMETERS OF SOILS UNDER DIFFERENT USES IN AN ALLUVIAL VALLEY Full text
2019
Lopes, Iug | Montenegro, Abelardo Antônio de Assunção
ABSTRACT Evaluating spatial variability of hydraulic properties and salinity of soils is important for an adequate agricultural management of alluvial soils, and protection of riparian vegetation. Thus, the objective of this work was to evaluate the accuracy of geophysical techniques for indirect measurements of apparent electrical conductivity (ECa), using an electromagnetic induction equipment (EM38®), and soil physical hydraulic parameters and their spatial interrelations. The study was carried out at the Advanced Research Unit of the UFRPE, in the Brígida River Basin, in Panamirim, state of Pernambuco, Brazil, in the second half of 2016. This river had a 100 m wide riparian forest strip transversely to the river bank on both sides of the river. A regular 20×10 m grid with 80 points was used to evaluate the soil hydraulic conductivity and ECa. The geostatistics showed the spatial dependence and the dependence of the soil attributes, their spatialization, and precise mapping through indirect readings. Most of the variability (86%) in soil electrical conductivity was explained by indirect readings using the EM38®. Ranges of 80 m, 380 m, and 134 m were found for soil moisture, ECa, and hydraulic conductivity, respectively, presenting strong spatial dependence. The results showed the importance of riparian forests to the maintenance of soil moisture and porosity to the improvement of soil water infiltration capacity even under severe water deficit conditions and soil subsurface layers. | RESUMO A caracterização da estrutura de variabilidade espacial de propriedades hidráulicas e da salinidade do solo é de grande importância para um adequado manejo agrícola de vales aluviais e para proteção da vegetação ciliar. Dessa forma, o objetivo deste trabalho foi a verificação da precisão de medições indiretas da condutividade elétrica aparente (CEa) utilizando o EM38®, bem como de parâmetros físico-hídricos do solo, e suas inter-relações espaciais. O estudo foi desenvolvido na Unidade Avançada de Pesquisa da UFRPE, localizada na Bacia do Rio Brígida, município de Panamirim-PE, no período do segundo semestre de 2016, possuindo uma faixa de mata ciliar de 100 m de largura, transversalmente a cada margem do rio. Adotou-se uma área com malha regular 20 x 10 m, totalizando 80 pontos, onde foram feitas avaliações da condutividade hidráulica e a calibração da CEa. A utilização da geoestatística permitiu identificar a dependência espacial e a dependência de todos atributos estudados, possibilitando sua espacialização e a utilização das leituras indiretas no mapeamento de precisão. Verificou-se que 86% da variabilidade da condutividade elétrica do solo pode ser explicada pelas leituras indiretas com o equipamento de indução eletromagnética (EM38®). Os valores de alcance foram de 80 m, 380 m e 134 m foram obtidas para a umidade do solo, CEa e condutividade hidráulica, respectivamente, as quais apresentaram forte dependência espacial. Verificou-se a relevância da mata ciliar na manutenção de umidade e porosidade do solo e, principalmente, contribuição para uma maior capacidade de infiltração do solo, mesmo para condições severas de escassez, e para as camadas sub-superficiais do mesmo.
Show more [+] Less [-]GENETIC DIVERSITY ASSESSMENT AMONG TALL COCONUT PALM Full text
2018
SOBRAL, KAMILA MARCELINO BRITO | QUEIROZ, MANOEL ABÍLIO DE | LEDO, CARLOS ALBERTO DA SILVA | LOIOLA, CARINA MENDES | ANDRADE, JÉSSICA BARROS | RAMOS, SEMÍRAMIS RABELO RAMALHO
ABSTRACT The tall coconut (Cocos nucifera L.) has great socioeconomic importance in Brazil and was first introduced on the coast of the north-eastern region, where it has been exploited in a semi-extractivist manner. The goal of this study was to quantify the genetic divergence between accessions introduced and preserved at the International Coconut Genebank for Latin America and the Caribbean, estimate the efficiency of descriptors used in the discrimination of the accessions, and indicate the essential descriptors for the activities of characterisation and evaluation. The accessions used were: Polynesia Tall; Tonga Tall; West African Tall; Rennel Tall; Rotuma Tall; Vanuatu Tall; Malayan Tall and Brazilian Tall Praia-do-Forte. Thirty-five quantitative descriptors recommended for the species were used. Genetic divergence was estimated by the Mahalanobis’s generalised distance and the cluster analysis was performed using the unweighted pair group method with arithmetic mean (UPGMA). The relative importance of the descriptors was measured according to Singh and Jolliffe’s methods, and the variables were selected taking into consideration the matching information in the two methods, eliminating those that were discarded in the two procedures. The agronomic characteristics indicated that the first canonical variable explained 90.25% of total variance. The most efficient descriptors for detecting the genetic divergence were: fruit equatorial circumference; nut polar and equatorial circumference; quantity of liquid endosperm; total fruit weight; nut weight; stem height; girth of stem at 1,5m height; number of leaflets; and number of bunches. The most dissimilar accessions according to the agronomic characteristics were Rotuma Tall and West African Tall, which can be primarily indicated as genitors for the formation of segregating populations in breeding programmes. | RESUMO O coqueiro-gigante (Cocos nucifera L.) tem grande importância socioeconômica no Brasil e foi primeiramente introduzido no litoral da região Nordeste, onde é explorado de forma semi-extrativista. O objetivo desse trabalho foi quantificar a divergência genética entre os acessos introduzidos e conservados no Banco Internacional de Germoplasma de Coco para América Latina e Caribe, verificar a eficiência dos descritores utilizados na discriminação dos acessos e indicar os essenciais para etapas de caracterização e avaliação. Foram avaliados os acessos: Gigante-da-Polinésia; Gigante-de-Tonga; Gigante-do-Oeste-Africano; Gigante-de-Rennel; Gigante-de-Rotuma; Gigante-de-Vanuatu; Gigante-da-Malásia e Gigante-da-Praia-do-Forte. Utilizou-se 35 descritores quantitativos recomendados para a espécie. A divergência genética foi estimada pela distância generalizada de Mahalanobis e o agrupamento foi realizado pelo método UPGMA. A importância relativa dos descritores foi mensurada de acordo com os métodos de Singh e Jolliffe e as variáveis foram selecionadas levando-se em consideração as informações coincidentes nos dois métodos, eliminando-se aqueles que foram descartados em ambos os procedimentos. Utilizando as características agronômicas detectou-se que a primeira variável canônica explicou 90,25% da variância total. Os descritores mais eficientes para detecção da divergência genética foram: circunferência equatorial do fruto, circunferência polar e equatorial da noz, volume do endosperma, peso total do fruto e noz, altura de estipe, circunferência do estipe a 150 cm do solo, número de folíolos e número de cachos por planta. Os acessos mais dissimilares pelas características agronômicas foram o Gigante-de-Rotuma e Gigante-do-Oeste-Africano, os quais podem ser prioritariamente indicados como genitores para a formação de populações segregantes em programas de melhoramento.
Show more [+] Less [-]FRUIT YIELD AND NUTRITIONAL CHARACTERISTICS OF SWEET PEPPER GROWN UNDER SALT STRESS IN HYDROPONIC SYSTEM Full text
2018
LIMA, NADIELAN DA SILVA | SILVA, ÊNIO FARIAS DE FRANÇA E | MENEZES, DIMAS | CAMARA, TEREZINHA RANGEL | WILLADINO, LILIA GOMES
ABSTRACT Irrigation with brackish water is common in the semiarid region of the Brazilian Northeast, potentiating the process of salinization of the soil in these areas. The hydroponic system stands out as an important strategy for crop management in semiarid regions, since crops show greater tolerance to the salt stress under hydroponic conditions, which minimize the effects of soil salinization. Thus, the objective of this work was to evaluate the effects of salt stress on the growth, fruit yield and mineral nutrition of sweet pepper in hydroponic system, using brackish water and coconut fiber substrate. The experiment was conducted in Recife, State of Pernambuco, Brazil (8°3'15''S, 34°52'53''W). A randomized block experimental design in a 5x2 factorial arrangement with five replications was used, consisting of five water salinity levels of the nutrient solution (2.0; 3.5; 4.5; 5.5 and 6.5 dS m-1) and two sweet pepper cultivars (Bruno and Rubia). The increasing salinity level of the nutrient solution decreased the fruit yield of the cultivars evaluated, reduced the potassium, phosphorus and sulfur, and increased the sodium and chlorine contents in the leaf tissues. The Ca contents of the cultivar Bruno decreased with increasing salinity, and increased in the cultivar Rubia. The cultivar Rubia stood out with the highest fruit yield and ionic selectivity, accumulating high amounts of potassium and calcium and maintaining low Na and Cl contents. The fruit yield of the cultivar Rubia (39.9 t ha-1) grown under solution with electrical conductivity of up to 3.5 dS m-1, denotes the potential viability of growing this cultivar under hydroponic conditions. | RESUMO A irrigação com águas salobra, frequente na região semiárida do nordeste brasileiro, potencializa o processo de salinização do solo nessas áreas. O cultivo hidropônico destaca-se como uma importante estratégia de convivência com o semiárido, uma vez que as culturas apresentam maior tolerância ao estresse salino nestas condições de cultivo, as quais minimizam os efeitos da salinização dos solos. O objetivo deste trabalho foi avaliar os efeitos da salinidade sobre o crescimento, produção e nutrição mineral de pimentão em cultivo hidropônico utilizando água salina em substrato de fibra de coco. O experimento foi conduzido em Recife, Pernambuco, Brasil (8°3'15'' S, 34°52'53'' W). Foi utilizado o delineamento experimental em blocos casualizados com fatorial 5x2, com cinco repetições, consistindo em cinco níveis de salinidade da solução nutritiva (2,0; 3,5; 4,5; 5,5 e 6,5 dS m-1) e duas cultivares de pimentão (Bruno e Rúbia). O incremento dos níveis de salinidade da solução nutritiva diminuiu a produção de frutos das cultivares avaliadas, reduziu os teores de potássio, fósforo e enxofre e aumentou os teores de sódio e cloro no tecido foliar. O teor de Ca da cv. Bruno decresceu em função do aumento da salinidade, e a apresentou aumento na cv. Rúbia. A cv. Rúbia destacou-se pela maior produtividade e maior eficiência na seletividade iônica, sendo capaz de acumular mais potássio e cálcio, e manter menores teores de Na e Cl. A produtividade da cv. Rúbia (39,9 t ha-1) quando cultivada em solução com condutividade elétrica de até 3,5 dS m-1 , indica a viabilidade potencial de seu cultivo em condições hidropônicas.
Show more [+] Less [-]GAS EXCHANGE AND PHOTOCHEMICAL EFFICIENCY IN LIMA BEAN GENOTYPES GROWN IN COMPACTED SOILS Full text
2018
SOUZA, RENATO FRANCISCO DA SILVA | SANTOS, DJAIL | PEREIRA, WALTER ESFRAIN | MACEDO, FABRÍCIO LOPES DE | VENDRUSCOLO, JHONY
RESUMO Os efeitos da compactação do solo no crescimento e produtividade das culturas têm sido muito estudados nos últimos anos, porém, as respostas fisiológicas das culturas à compactação não têm recebido a devida atenção. Objetivou-se avaliar o efeito da compactação do solo nas trocas gasosas e na eficiência fotoquímica de genótipos de fava (Phaseolus lunatus L.) de diferentes hábitos de crescimento. O delineamento experimental utilizado foi em blocos casualizados no arranjo fatorial 3 × 4, sendo três genótipos de fava (Branca pequena, Orelha de vó e Roxinha) e quatro níveis de compactação (densidades de solo de 1,1; 1,3; 1,5 e 1,7 g cm-3), com quatro repetições. Aos 38 dias após a semeadura, analisaram-se as seguintes variáveis: taxa fotossintética (A), transpiração foliar (E), condutância estomática (gs), concentração interna de CO2 (Ci), eficiência instantânea no uso da água (EUA), eficiência intrínseca no uso da água (EIUA), eficiência instantânea de carboxilação (EiC) e eficiência fotoquímica (Fo, Fm, Fv e Fv/Fm). Os dados foram submetidos à análise de variância a 5% de probabilidade pelo teste F. Os genótipos apresentaram redução na taxa fotossintética com aumento da compactação do solo. Houve efeito da compactação do solo na eficiência fotoquímica apenas para o genótipo ‘Orelha de vó’, com ajustes do modelo linear para Fm e Fv, e quadrático para relação Fv/Fm. O genótipo ‘Orelha de vó’ atingiu a maior taxa de E e gs nas densidades de solo 1,24 e 1,29 g cm-3, respectivamente. Em relação a taxa fotossintética, o genótipo ‘Roxinha’ é mais eficiente que o genótipo ‘Branca pequena’ na densidade do solo de 1,7 g cm-3. | ABSTRACT The effects of soil compaction on crop growth and productivity have been well studied in recent years, however, studies on the physiological responses of crops to compaction are scarce. The objective of this study was to evaluate the effect of soil compaction on gas exchange, and photochemical efficiency of lima bean (Phaseolus lunatus L.) genotypes of different growth habits. The experimental design was a randomized block in a 3×4 factorial arrangement, with three lima bean genotypes (Branca-Pequena, Orelha-de-Vó and Roxinha) and four compaction levels (soil densities of 1.1, 1.3, 1.5 and 1.7 g cm-3), with four replications. The following variables were evaluated at 38 days after sowing: photosynthetic rate (A), leaf transpiration (E), stomatal conductance (gs), internal CO2 concentration (Ci), instantaneous water use efficiency (WUE), intrinsic water use efficiency (iWUE), instantaneous carboxylation efficiency (iCE) and photochemical efficiency (Fo, Fm, Fv and Fv/Fm). The data were subjected to analysis of variance at 5% probability by the F test. The genotypes showed a reduction in the photosynthetic rate with increasing soil compaction. The soil compaction affected the photochemical efficiency of the genotype Orelha-de-Vó, with the Fm and Fv fitting to the linear model, and the Fv/Fm fitting to the quadratic model. The genotype Orelha-de-Vó had the highest rate of E and gs at the soil densities of 1.24 and 1.29 g cm-3, respectively. Regarding the photosynthetic rate, the genotype Roxinha is more efficient than Branca-Pequena at the soil density of 1.7 g cm -3.
Show more [+] Less [-]GENETIC DIVERGENCE AMONG COWPEA GENOTYPES BASED ON CHARACTERS ASSOCIATED WITH THE GREEN POD AND GRAIN MARKET Full text
2018
TORRES FILHO, JOSÉ | OLIVEIRA, CHRISTIANE NORONHA GOMES DOS SANTOS | SILVEIRA, LINDOMAR MARIA DA | NUNES, GLAUBER HENRIQUE DE SOUSA | PEREIRA, CARLA CAROLINE ALVES | SILVA, ANTÔNIA ELIZIANA AUGUSTA DA
GENETIC DIVERGENCE AMONG COWPEA GENOTYPES BASED ON CHARACTERS ASSOCIATED WITH THE GREEN POD AND GRAIN MARKET Full text
2018
TORRES FILHO, JOSÉ | OLIVEIRA, CHRISTIANE NORONHA GOMES DOS SANTOS | SILVEIRA, LINDOMAR MARIA DA | NUNES, GLAUBER HENRIQUE DE SOUSA | PEREIRA, CARLA CAROLINE ALVES | SILVA, ANTÔNIA ELIZIANA AUGUSTA DA
ABSTRACT The objective of this study was to evaluate genetic divergence among cowpea genotypes and to select parents for crosses aimed at the fresh pod and grain market. Two experiments were carried out during 2014, corresponding to two sowing times, in the municipality of Mossoró, State of Rio Grande do Norte. Twenty-three cowpea genotypes were evaluated in a randomized complete block design with four replicates. Fifteen descriptors were used to quantify divergence, using the Mahalanobis distance as a measure of dissimilarity, obtained from the genotypic mean predicted by the Restricted Maximum Likelihood/Best Linear Unbiased Prediction (REML/BLUP) method. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) hierarchical method was used to group genotypes and the Singh criterion to quantify the contribution of traits to genetic divergence. The genotype × environment interaction (G × E) influenced divergence, both in the contribution of traits and in the grouping of genotypes. In the experiment 1, the genotypes were distributed among four groups. In the experiment 2, less discrimination occurred and the genotypes were represented by only two groups. When joint analysis of two evaluations was carried out based on two sowing times, genotypes were represented by six groups. The G × E interaction influences the contribution of traits and the grouping of cowpea genotypes in the study of divergence. The genetic divergence of the set of cowpea genotypes evaluated is mainly due to green grain and pod yield. BRS Aracê and BRS Xiquexique cultivars are the most divergent among the genotypes studied, representing 75% of the recommended crosses. | RESUMO O objetivo deste trabalho foi avaliar a divergência genética entre genótipos de feijão-caupi e selecionar genitores para cruzamentos visando o mercado de vagens e grãos verdes. Foram conduzidos dois experimentos, correspondendo a duas épocas de semeadura, no município de Mossoró, Rio Grande do Norte, ano 2014. Foram avaliados 23 genótipos de feijão-caupi em delineamento de blocos casualizados com quatro repetições. Foram utilizados 15 descritores para quantificar a divergência, utilizando como medida de dissimilaridade a distância de Mahalanobis, obtida a partir da média genotípica predita pelo método REML/BLUP. Utilizou-se o método hierárquico UPGMA para agrupar os genótipos e o critério de Singh para quantificar a contribuição dos caracteres para a divergência genética. A interação genótipos x ambientes influenciou a divergência, tanto na contribuição dos caracteres quanto no agrupamento dos genótipos. Na primeira época de cultivo os genótipos foram distribuídos em quatro grupos. Na segunda época ocorreu menor discriminação, sendo que os genótipos formaram apenas dois grupos. Ao realizar-se o agrupamento com base na análise conjunta das duas avaliações, realizadas nas duas épocas de semeadura os genótipos foram agrupados em seis grupos. A interação genótipos x ambientes influencia a contribuição das características e o agrupamento dos genótipos de feijão-caupi no estudo de divergência. A divergência genética do conjunto de genótipos de feijão-caupi avaliados é devida principalmente a produtividades de grãos verdes e de vagens verdes. As cultivares BRS Aracê e BRS Xiquexique foram os genótipos mais divergentes entre os estudados, fazendo parte de 75% dos cruzamentos recomendados.
Show more [+] Less [-]GENETIC DIVERGENCE AMONG COWPEA GENOTYPES BASED ON CHARACTERS ASSOCIATED WITH THE GREEN POD AND GRAIN MARKET Full text
2018
JOSÉ TORRES FILHO | CHRISTIANE NORONHA GOMES DOS SANTOS OLIVEIRA | LINDOMAR MARIA DA SILVEIRA | GLAUBER HENRIQUE DE SOUSA NUNES2 | CARLA CAROLINE ALVES PEREIRA | ANTÔNIA ELIZIANA AUGUSTA DA SILVA
The objective of this study was to evaluate genetic divergence among cowpea genotypes and to select parents for crosses aimed at the fresh pod and grain market. Two experiments were carried out during 2014, corresponding to two sowing times, in the municipality of Mossoró, State of Rio Grande do Norte. Twenty-three cowpea genotypes were evaluated in a randomized complete block design with four replicates. Fifteen descriptors were used to quantify divergence, using the Mahalanobis distance as a measure of dissimilarity, obtained from the genotypic mean predicted by the Restricted Maximum Likelihood/Best Linear Unbiased Prediction (REML/BLUP) method. The Unweighted Pair Group Method with Arithmetic Mean (UPGMA) hierarchical method was used to group genotypes and the Singh criterion to quantify the contribution of traits to genetic divergence. The genotype × environment interaction (G × E) influenced divergence, both in the contribution of traits and in the grouping of genotypes. In the experiment 1, the genotypes were distributed among four groups. In the experiment 2, less discrimination occurred and the genotypes were represented by only two groups. When joint analysis of two evaluations was carried out based on two sowing times, genotypes were represented by six groups. The G × E interaction influences the contribution of traits and the grouping of cowpea genotypes in the study of divergence. The genetic divergence of the set of cowpea genotypes evaluated is mainly due to green grain and pod yield. BRS Aracê and BRS Xiquexique cultivars are the most divergent among the genotypes studied, representing 75% of the recommended crosses.
Show more [+] Less [-]GENOTYPE BY ENVIRONMENT INTERACTION IN COWPEA LINES USING GGE BIPLOT METHOD Full text
2018
SOUSA, MASSAINE BANDEIRA E | DAMASCENO-SILVA, KAESEL JACKSON | ROCHA, MAURISRAEL DE MOURA | MENEZES JÚNIOR, JOSÉ ÂNGELO NOGUEIRA DE | LIMA, LAÍZE RAPHAELLE LEMOS
GENOTYPE BY ENVIRONMENT INTERACTION IN COWPEA LINES USING GGE BIPLOT METHOD Full text
2018
SOUSA, MASSAINE BANDEIRA E | DAMASCENO-SILVA, KAESEL JACKSON | ROCHA, MAURISRAEL DE MOURA | MENEZES JÚNIOR, JOSÉ ÂNGELO NOGUEIRA DE | LIMA, LAÍZE RAPHAELLE LEMOS
RESUMO O método GGE Biplot é eficiente em identificar genótipos favoráveis e ambientes ideais para avaliação. Portanto, o objetivo deste trabalho foi avaliar a interação genótipo por ambientes (G×A) de linhagens elite de feijão-caupi nos ensaios de valor de cultivo e uso (VCU) da Embrapa Meio-Norte, realizados nas regiões de Cerrado do Brasil, por meio de analises via GGE Biplot. Avaliou-se a produtividade de grãos em 40 genótipos de feijão-caupi, sendo 30 linhagens e 10 cultivares, durante três anos (2010, 2011 e 2012) em três locais: Balsas (BAL), São Raimundo das Mangabeiras (SRM) e Primavera do Leste (PRL). Os dados foram submetidos a análises de variância, a partir da qual foram obtidas as médias ajustadas para realizar a análise via GGE-Biplot. Os resultados gráficos revelam que houve variação no comportamento dos genótipos nos locais avaliados ao longo dos anos. As linhagens MNC02-675F-4-9 e MNC02-675F-4-10 apresentaram desempenhos de um genótipo ideal, com máxima produtividade aliada à boa estabilidade nos locais de avaliação. Houve a formação de três mega-ambientes que englobaram ambientes correlacionados positivamente. As linhagens MNC02-675F-4-9, MNC02-675F-9-3 e MNC02-701F-2 apresentaram o melhor desempenho médio dentro de cada mega-ambiente. O ambiente PRL10 e as linhagens próximas a este ambiente, como MNC02-677F-2, MNC02-677F-5 e a cultivar testemunha BRS-Marataoã, puderam ser classificados com maior confiabilidade, determinados basicamente pelos efeitos genotípicos, com G×A reduzida. A maioria dos ambientes avaliados foram ideais para avaliação da G×A, discriminando bem os genótipos. Portanto, a análise GGE-Biplot, permitiu selecionar genótipos com adaptabilidade e desempenhos superiores para ambientes específicos. | ABSTRACT The GGE Biplot method is efficien to identify favorable genotypes and ideal environments for evaluation. Therefore, the objective of this work was to evaluate the genotype by environment interaction (G×E) and select elite lines of cowpea from genotypes, which are part of the cultivation and use value tests of the Embrapa Meio-Norte Breeding Program, for regions of the Brazilian Cerrado, by the GGE-Biplot method. The grain yield of 40 cowpea genotypes, 30 lines and 10 cultivars, was evaluated during three years (2010, 2011 and 2012) in three locations: Balsas (BAL), São Raimundo das Mangabeiras (SRM) and Primavera do Leste (PRL). The data were subjected to analysis of variance, and adjusted means were obtained to perform the GGE-Biplot analysis. The graphic results showed variation in the performance of the genotypes in the locations evaluated over the years. The performance of the lines MNC02-675F-4-9 and MNC02-675F-4-10 were considered ideal, with maximum yield and good stability in the locations evaluated. There mega-environments were formed, encompassing environments correlated positively. The lines MNC02-675F-4-9, MNC02-675F-9-3 and MNC02-701F-2 had the best performance within each mega-environment. The environment PRL10 and lines near this environment, such as MNC02-677F-2, MNC02-677F-5 and the control cultivar (BRS-Marataoã) could be classified as those of greater reliability, determined basically by the genotypic effects, with reduced G×E. Most of the environments evaluated were ideal for evaluation of G×E, since the genotypes were well discriminated on them. Therefore, the selection of genotypes with adaptability and superior performance for specific environments through the GGE-Biplot analysis was possible.
Show more [+] Less [-]GENOTYPE BY ENVIRONMENT INTERACTION IN COWPEA LINES USING GGE BIPLOT METHOD Full text
2018
MASSAINE BANDEIRA E SOUSA | KAESEL JACKSON DAMASCENO-SILVA | MAURISRAEL DE MOURA ROCHA | JOSÉ ÂNGELO NOGUEIRA DE MENEZES JÚNIOR | LAÍZE RAPHAELLE LEMOS LIMA
The GGE Biplot method is efficien to identify favorable genotypes and ideal environments for evaluation. Therefore, the objective of this work was to evaluate the genotype by environment interaction (G×E) and select elite lines of cowpea from genotypes, which are part of the cultivation and use value tests of the Embrapa Meio-Norte Breeding Program, for regions of the Brazilian Cerrado, by the GGE-Biplot method. The grain yield of 40 cowpea genotypes, 30 lines and 10 cultivars, was evaluated during three years (2010, 2011 and 2012) in three locations: Balsas (BAL), São Raimundo das Mangabeiras (SRM) and Primavera do Leste (PRL). The data were subjected to analysis of variance, and adjusted means were obtained to perform the GGE-Biplot analysis. The graphic results showed variation in the performance of the genotypes in the locations evaluated over the years. The performance of the lines MNC02-675F-4-9 and MNC02-675F-4-10 were considered ideal, with maximum yield and good stability in the locations evaluated. There mega-environments were formed, encompassing environments correlated positively. The lines MNC02-675F-4-9, MNC02-675F-9-3 and MNC02-701F-2 had the best performance within each mega-environment. The environment PRL10 and lines near this environment, such as MNC02-677F-2, MNC02-677F-5 and the control cultivar (BRS-Marataoã) could be classified as those of greater reliability, determined basically by the genotypic effects, with reduced G×E. Most of the environments evaluated were ideal for evaluation of G×E, since the genotypes were well discriminated on them. Therefore, the selection of genotypes with adaptability and superior performance for specific environments through the GGE-Biplot analysis was possible.
Show more [+] Less [-]