Rice is one of the world’s most consumed cereals, however, its production is affected by fungal diseases. Thus, the objective of this work was to evaluate the severity of diseases and grain yield potential of upland rice with silicon fertilization combined with seed microbiolization with Bacillus methylotrophicus isolates. Two experiments were conducted, one in Igarapé do Meio, Maranhão (MA), Brazil, with rice seeds of the variety Palha-Murcha and one in São Bento MA with rice seeds of the variety BRS-Primavera. A randomized block experimental design in a split-plot arrangement with five replications were used in both experiments, with agro-silicon rates (0.0, 1.0, 2.0, 4.0 and 6.0 Mg ha-1) in the plots and microbiolized and non-microbiolized rice seeds with B. Methylotrophicus at concentration of 108 CFU ml-1 in the subplots. The seedling emergence, grain yield, number of panicles, plant height, plant dry weight and severity of brown leafspot, leaf scald and grain spot were evaluated. Soil fertilization with agro-silicon affected positively the plant height of the variety Palha-Murcha and the number of panicles, plant dry weight and grain yield of the variety BRS-Primavera, and negatively the germination of the variety BRS-Primavera. Leaf scald severity in the variety BRS-Primavera reduced with microbiolized seeds with B. methylotropicus. Microbiolization with B. methylotropicus had no effect on severity of brown leafspot and grain spot in the varieties evaluated.

ABSTRACT Rice is one of the world’s most consumed cereals, however, its production is affected by fungal diseases. Thus, the objective of this work was to evaluate the severity of diseases and grain yield potential of upland rice with silicon fertilization combined with seed microbiolization with Bacillus methylotrophicus isolates. Two experiments were conducted, one in Igarapé do Meio, Maranhão (MA), Brazil, with rice seeds of the variety Palha-Murcha and one in São Bento MA with rice seeds of the variety BRS-Primavera. A randomized block experimental design in a split-plot arrangement with five replications were used in both experiments, with agro-silicon rates (0.0, 1.0, 2.0, 4.0 and 6.0 Mg ha-1) in the plots and microbiolized and non-microbiolized rice seeds with B. Methylotrophicus at concentration of 108 CFU ml-1 in the subplots. The seedling emergence, grain yield, number of panicles, plant height, plant dry weight and severity of brown leafspot, leaf scald and grain spot were evaluated. Soil fertilization with agro-silicon affected positively the plant height of the variety Palha-Murcha and the number of panicles, plant dry weight and grain yield of the variety BRS-Primavera, and negatively the germination of the variety BRS-Primavera. Leaf scald severity in the variety BRS-Primavera reduced with microbiolized seeds with B. methylotropicus. Microbiolization with B. methylotropicus had no effect on severity of brown leafspot and grain spot in the varieties evaluated. | RESUMO O arroz é um dos cereais mais consumidos em todo o mundo e sua produção é afetada por doenças fúngicas. Neste sentido objetivou-se avaliar a severidade de doenças e o potencial produtivo do arroz de terras altas em função da aplicação de silício e sementes microbiolizadas com isolados de Bacillus methylotrophicus. Foram realizados dois experimentos, em dois munícipios do estado do Maranhão, Brasil: um em Igarapé do Meio, com sementes de arroz da variedade Palha Murcha e outro em São Bento, com a variedade Primavera. Os experimentos foram instalados no delineamento em blocos casualizados, com cinco repetições, em parcelas subdivididas. As parcelas foram constituídas por cinco doses de agrosilício (0,0; 1,0; 2,0; 4,0 e 6,0 t ha-1) e as subparcelas foram constituídas por sementes de arroz microbiolizadas e não microbiolizadas com B. methylotrophicus, na concentração de 108 UFC ml-1. Os parâmetros avaliados foram emergência de plântulas, produtividade de grãos, número de panícula, altura de planta, massa de planta seca e severidade de mancha parda, escaldadura e mancha de grãos. As doses de agrosilício aplicadas no solo afetaram positivamente a altura de plantas, para a variedade Palha Murcha. Na variedade Primavera, as doses de agrosilício influenciaram de forma positiva o número de panículas, massa de planta seca e a produtividade de grãos e de forma negativa a germinação. Para esta variedade, a severidade da escaldadura foi reduzida quando as sementes foram microbiolizadas com B. methylotropicus. A microbiolização com B. methylotropicus não influenciou a severidade da mancha parda e a severidade da mancha de grãos, para as duas variedades de arroz.

The tropical dry areas have suffered the most severe anthropic pressures. This factor motivates studies aimed at characterizing and monitoring the soil quality to determine the management measures to apply and to suggest appropriate recovery procedures. The objective of this study was to evaluate the enzymatic activity of acid and alkaline phosphatases, urea and arylsulfatase in the superficial layers of soils in areas under different stages of forest regeneration, in Floresta-PE City.. Soil sampling was conducted at 0-5, 5-15 and 15-30 cm layer in the following areas: C-18 (vegetation regeneration for 18 years), P-13 (13 years), L-12 (12 years), C-9 (9 years), C-7 (7 years) and G-4 (4 years). The analytical methods used in the study were based on the incubation of the soil samples with a buffered solution of substrate specific for each enzyme. The methods were based on colorimetric determinations. The activity of acid and alkaline phosphatase and urease represented sensitive measures for detecting changes in soil quality at various stages of regeneration in tropical dry forests. The area with the longest duration of forest regeneration (C-18) showed higher enzyme activities. The soil enzymatic activities respond to different stages and management of forest regeneration in Brazilian tropical dry areas, namely Caatinga. The acid and alkaline phosphatase, arylsulfatase and urease increased with time of regeneration.

ABSTRACT The tropical dry areas have suffered the most severe anthropic pressures. This factor motivates studies aimed at characterizing and monitoring the soil quality to determine the management measures to apply and to suggest appropriate recovery procedures. The objective of this study was to evaluate the enzymatic activity of acid and alkaline phosphatases, urea and arylsulfatase in the superficial layers of soils in areas under different stages of forest regeneration, in Floresta-PE City.. Soil sampling was conducted at 0-5, 5-15 and 15-30 cm layer in the following areas: C-18 (vegetation regeneration for 18 years), P-13 (13 years), L-12 (12 years), C-9 (9 years), C-7 (7 years) and G-4 (4 years). The analytical methods used in the study were based on the incubation of the soil samples with a buffered solution of substrate specific for each enzyme. The methods were based on colorimetric determinations. The activity of acid and alkaline phosphatase and urease represented sensitive measures for detecting changes in soil quality at various stages of regeneration in tropical dry forests. The area with the longest duration of forest regeneration (C-18) showed higher enzyme activities. The soil enzymatic activities respond to different stages and management of forest regeneration in Brazilian tropical dry areas, namely Caatinga. The acid and alkaline phosphatase, arylsulfatase and urease increased with time of regeneration. | RESUMO As áreas tropicais secas são as que sofrem as mais severas pressões antrópicas. Este fator motiva estudos que objetivam caracterizar e monitorar a qualidade do solo visando determinar estratégias de manejo para os produtores. O objetivou-se avaliar a atividade enzimática das enzimas fosfatases ácida e alcalina, uréase e arilsulfatase nas camadas superficiais de solos em áreas sob diferentes estágios de regeneração florestal, no município de Floresta-PE. Amostras de solos foram coletadas nas camadas 0-5, 5-15 e 15-30 cm nas áreas C-18 (regeneração por 18 anos), P-13 (13 anos), L-12 (12 anos), C-9 (9 anos), C-7 (7 anos) e G-4(4 anos). Os métodos analíticos usados neste estudo foram baseados na incubação das amostras de solo com soluções tamponadas de substratos específicos de cada enzima. Os métodos foram baseados em determinações colorimétricas. As atividades das fosfatases e uréase foram sensíveis em detectar mudanças na qualidade de solos com vários estágios de regeneração em áreas secas tropicais. As áreas com maior tempo de regeneração (C-18) mostraram maiores atividades enzimáticas. As atividades enzimáticas do solo respondem a diferentes estágios e manejo de regeneração florestal nas áreas secas tropicais brasileiras, nomeadamente Caatinga. A fosfatase ácida e alcalina, arilsulfatase e urease aumentaram com o tempo de regeneração.

The objective of this study was to evaluate the effects of the addition of parts of leguminous trees on the growth and nutrition of maize (Zea mays L.), as well as on the chemical attributes of a degraded soil, 65 days after applying the residues. The experiment was conducted in pots, in a randomized block design with ten treatments and four replicates. The evaluated treatments were: T1 - No residues of leguminous trees, T2 - Leaves of Mimosa caesalpiniifolia, T3 - Branches of Mimosa caesalpiniifolia, T4 - Leaves + branches of Mimosa caesalpiniifolia, T5 - Leaves of Mimosa hostilis, T6 - Branches of Mimosa hostilis, T7 - Leaves + branches of Mimosa hostilis, T8 - Leaves of Gliricidia sepium, T9 - Branches of Gliricidia sepium and T10 - Leaves + branches of Gliricidia sepium. Pots were filled with soil from a degraded area and residues were added in the form of green mass after sowing the maize. Residues of leguminous trees positively influenced maize growth at 65 days after application and favored the accumulation of nitrogen, phosphorus and potassium in the shoots. Green fertilizers improved soil chemical attributes at 65 days after application, especially nitrate-N (NO3 --N), ammonium-N (NH4 +-N), total inorganic N (NO3 --N + NH4 +-N) and K, demonstrating that these species are good options for recovering degraded areas in the semi-arid region of Ceará.

RESUMO Este estudo teve como objetivo avaliar o efeito da adição de resíduos de partes de leguminosas arbóreas no crescimento e nutrição de plantas de milho (Zea mays L.), bem como nos atributos químicos de um solo degradado, 65 dias após a aplicação dos resíduos. O experimento foi realizado em vasos, em delineamento de blocos ao acaso, com dez tratamentos e quatro repetições. Os tratamentos avaliados foram: T1 - Sem resíduo de leguminosas, T2 - Folhas de Mimosa caesalpiniifolia, T3 - Galhos de Mimosa caesalpiniifolia, T4 - Folhas + galhos de Mimosa caesalpiniifolia, T5 - Folhas de Mimosa hostilis, T6 - Galhos de Mimosa hostilis, T7 - Folhas + galhos de Mimosa hostilis, T8 - Folhas de Gliricidia sepium, T9 - Galhos de Gliricidia sepium e T10 - Folhas + galhos de Gliricidia sepium. Os vasos foram preenchidos com solo de área degradada e os resíduos adicionados na forma de massa verde após a semeadura do milho. Resíduos de leguminosas arbóreas influenciaram positivamente o crescimento de plantas de milho já aos 65 dias após a aplicação e favoreceram o acúmulo de nitrogênio, fósforo e potássio na parte aérea de plantas. Os adubos verdes utilizados melhoraram atributos químicos do solo logo aos 65 dias após a aplicação, com destaque para o N-nitrato (N-NO3 -), N-amônio (N-NH4 +), nitrogênio inorgânico total (N-NO3 - + N-NH4 +) e K, demonstrando que essas espécies são boas opções para recuperação de áreas degradadas no semiárido cearense. | ABSTRACT The objective of this study was to evaluate the effects of the addition of parts of leguminous trees on the growth and nutrition of maize (Zea mays L.), as well as on the chemical attributes of a degraded soil, 65 days after applying the residues. The experiment was conducted in pots, in a randomized block design with ten treatments and four replicates. The evaluated treatments were: T1 - No residues of leguminous trees, T2 - Leaves of Mimosa caesalpiniifolia, T3 - Branches of Mimosa caesalpiniifolia, T4 - Leaves + branches of Mimosa caesalpiniifolia, T5 - Leaves of Mimosa hostilis, T6 - Branches of Mimosa hostilis, T7 - Leaves + branches of Mimosa hostilis, T8 - Leaves of Gliricidia sepium, T9 - Branches of Gliricidia sepium and T10 - Leaves + branches of Gliricidia sepium. Pots were filled with soil from a degraded area and residues were added in the form of green mass after sowing the maize. Residues of leguminous trees positively influenced maize growth at 65 days after application and favored the accumulation of nitrogen, phosphorus and potassium in the shoots. Green fertilizers improved soil chemical attributes at 65 days after application, especially nitrate-N (NO3 --N), ammonium-N (NH4 +-N), total inorganic N (NO3 --N + NH4 +-N) and K, demonstrating that these species are good options for recovering degraded areas in the semi-arid region of Ceará.

Knowledge of the nutrient uptake by crops at different stages of growth may provide the basis for rational application of fertilizers, which may lead to reduced production costs and improved use of fertilizers by plants. Based on this knowledge, we conducted this study to determine the biomass and nutrient accumulation curve of the onion cultivar Bella Vista in a system of transplanted seedlings. The experiment was carried out in Ituporanga-SC, Brazil, by using a randomized block design with three replicates. Eleven plant collections were carried out throughout the crop cycle. The variables evaluated included plant length; total dry biomass of leaves, bulbs, and roots; and concentrations of macro- and micronutrients. The maximum dry biomass accumulation in the shoots and bulbs at 140 days after transplanting (DAT) was 4.26 and 27.41 mg per plant, respectively, which contributed to 13% and 85% of the whole plant dry biomass at the end of the cycle, respectively. Potassium (K, 521.6 mg) was accumulated in the largest quantity per plant, followed by nitrogen (N, 465.7 mg), calcium (Ca, 253.3 mg), sulfur (S, 109.0 mg), magnesium (Mg, 86.9 mg), and phosphorus (P, 76.5 mg). The micronutrient accumulation was as follows: iron (Fe, 6,227.5 µg), boron (B, 902.3 µg), zinc (Zn, 573.7 µg), manganese (Mn, 573.7 µg), and copper (Cu, 241.4 µg).

ABSTRACT Knowledge of the nutrient uptake by crops at different stages of growth may provide the basis for rational application of fertilizers, which may lead to reduced production costs and improved use of fertilizers by plants. Based on this knowledge, we conducted this study to determine the biomass and nutrient accumulation curve of the onion cultivar Bella Vista in a system of transplanted seedlings. The experiment was carried out in Ituporanga-SC, Brazil, by using a randomized block design with three replicates. Eleven plant collections were carried out throughout the crop cycle. The variables evaluated included plant length; total dry biomass of leaves, bulbs, and roots; and concentrations of macro- and micronutrients. The maximum dry biomass accumulation in the shoots and bulbs at 140 days after transplanting (DAT) was 4.26 and 27.41 mg per plant, respectively, which contributed to 13% and 85% of the whole plant dry biomass at the end of the cycle, respectively. Potassium (K, 521.6 mg) was accumulated in the largest quantity per plant, followed by nitrogen (N, 465.7 mg), calcium (Ca, 253.3 mg), sulfur (S, 109.0 mg), magnesium (Mg, 86.9 mg), and phosphorus (P, 76.5 mg). The micronutrient accumulation was as follows: iron (Fe, 6,227.5 µg), boron (B, 902.3 µg), zinc (Zn, 573.7 µg), manganese (Mn, 573.7 µg), and copper (Cu, 241.4 µg). | RESUMO O conhecimento da extração de nutrientes pelas culturas, em diferentes estádios, pode fornecer subsídios para aplicação racional de fertilizantes, resultando na redução dos custos de produção e melhor aproveitamento dos fertilizantes pelas plantas. Desta forma, objetivou-se com este trabalho determinar a curva de acúmulo de fitomassa e nutrientes para a cebola cv. Bella Vista, em sistema de mudas transplantadas. O experimento foi instalado e conduzido no município de Ituporanga - SC, Brasil, utilizando o delineamento experimental utilizado foi em bloco ao acaso com três repetições. Foram avaliados o comprimento da planta, fitomassa seca total, das folhas, do bulbo e raízes e concentração de macro e micronutrientes. A máxima fitomassa seca de da parte aérea e bulbos aos 140 dias após o transplante foi de 4,26 e 27,41 mg por planta, respectivamente, o que contribuiu para 13% e 85% da massa seca total da planta no final do ciclo, respectivamente. O potássio (521,6 mg) foi acumulado em maior quantidade pela planta, seguido pelo nitrogênio (N, 465,7 mg), cálcio (Ca, 253,3 mg), ensofre (S, 109,0 mg), magnésio (Mg, 86,9 mg) e fósforo (P, 76,5 mg). Para os micronutrientes a ordem de acúmulo foi: ferro (Fe, 6.227,5 µg), boro (B, 902,3 µg), zinco (Zn, 573,7 µg), manganês (Mn, 573,7 µg), e cobre (Cu, 241,4 µg).

Green manure may lead to physical, chemical, and biological improvements to the soil. However, the information on its use in the Brazilian Northeast is scarce. The aim of this study was to assess the phenological cycle and phytomass productivity of seven legume species. This study was conducted in the second semester of 2015 in a completely randomized design with seven treatments and four replications. Plant height, fresh mass of shoot (FMS), dry mass of shoot (DMS), fresh mass of root (FMR), and dry mass of root (DMR) were determined at 100 days after sowing. Germination, flowering, and pod maturation were also assessed. The highest averages of FMS were observed in Crotalaria breviflora and Crotalaria mucronata, followed by Canavalia ensiformis, whereas the highest averages of DMS were observed in C. breviflora, C. ensiformis, C. mucronata, and Cajanus cajan cv. IAPAR 43. The highest averages of FMR were obtained by C. breviflora and C. mucronata, followed by C. cajan cv. Fava Larga. The species C. mucronata, C. cajan cv. IAPAR 43, and C. breviflora presented the highest averages of DMR. In addition, C. juncea, C. ensiformis, and C. cajan cv. IAPAR 43 were the earlier species regarding flowering, while C. cajan cv. Fava Larga was the later species. C. juncea was the earliest among all studied species regarding pod maturation. The tested legume species obtained promising initial results during the assessment period, being able to meet the expectations of biomass production and contributing to soil sustainability in this region.

ABSTRACT Green manure may lead to physical, chemical, and biological improvements to the soil. However, the information on its use in the Brazilian Northeast is scarce. The aim of this study was to assess the phenological cycle and phytomass productivity of seven legume species. This study was conducted in the second semester of 2015 in a completely randomized design with seven treatments and four replications. Plant height, fresh mass of shoot (FMS), dry mass of shoot (DMS), fresh mass of root (FMR), and dry mass of root (DMR) were determined at 100 days after sowing. Germination, flowering, and pod maturation were also assessed. The highest averages of FMS were observed in Crotalaria breviflora and Crotalaria mucronata, followed by Canavalia ensiformis, whereas the highest averages of DMS were observed in C. breviflora, C. ensiformis, C. mucronata, and Cajanus cajan cv. IAPAR 43. The highest averages of FMR were obtained by C. breviflora and C. mucronata, followed by C. cajan cv. Fava Larga. The species C. mucronata, C. cajan cv. IAPAR 43, and C. breviflora presented the highest averages of DMR. In addition, C. juncea, C. ensiformis, and C. cajan cv. IAPAR 43 were the earlier species regarding flowering, while C. cajan cv. Fava Larga was the later species. C. juncea was the earliest among all studied species regarding pod maturation. The tested legume species obtained promising initial results during the assessment period, being able to meet the expectations of biomass production and contributing to soil sustainability in this region. | RESUMO A adubação verde pode atribuir melhorias físicas, químicas e biológicas aos solos, porém, são escassas as informações de uso no nordeste brasileiro. Objetivou-se avaliar o ciclo fenológico e as produtividades de fitomassa de sete espécies de leguminosas. O trabalho foi conduzido durante o segundo semestre de 2015, em delineamento inteiramente casualizado, com sete tratamentos e quatro repetições. Aos 100 dias após semeadura (DAS) foram determinadas a altura das plantas, massa fresca (MFPA) e seca da parte aérea (MSPA), massa fresca (MFR) e seca da raiz (MSR). Avaliou-se também a germinação, florescimento e maturação das vagens. As maiores médias para MFPA foram obtidas pelas Crotalaria breviflora e Crotalaria mucronata, seguidas da Canavalia ensiformis. As mais produtivas para MSPA foram C. breviflora, C. ensiformis, C. mucronata, e Cajanus cajan Cv IAPAR 43. As maiores médias para MFR foram obtidas pelas C. breviflora e C. mucronata, seguidas do C. cajan Cv Fava Larga. A C. mucronata, C. cajan Cv IAPAR 43 e C. breviflora foram as espécies que obtiveram as maiores médias de MSR. A Crotalaria juncea, C. ensiformis e C. cajan Cv IAPAR 43 foram as espécies mais precoces em relação à floração, enquanto que C. cajan Cv Fava Larga foi a espécie mais tardia. C. juncea foi a mais precoce, entre todas as espécies estudadas, para a variável maturação das vagens. As espécies de leguminosas testadas obtiveram, durante o período de avaliação, resultados iniciais promissores, podendo atender as expectativas de produção de biomassa, além de contribuir para a sustentabilidade dos solos desta região.

RESUMO Objetivou-se com este trabalho avaliar os parâmetros fitomorfológicos da cultura da soja em um sistema de integração lavoura-pecuária e inoculados com Azospirillum brasilense. O experimento foi conduzido em Santa Maria, em dois agrícolas (2012/2013 e 2013/2014), num delineamento experimental de blocos casualizados, com três repetições. No período de inverno a pastagem de aveia preta e azevém foi manejada com ovinos em diferentes sistemas de pastejo: (I) - pastejo convencional (PC), onde os animais permaneciam na pastagem por todo o período experimental sem um controle da altura da pastagem, (II,III e IV) - altura da pastagem na saída dos animais de 10, 20 e 30 cm e uma testemunha sem pastejo (SP), três doses de nitrogênio (0, 50 e 100 kg ha-1) e com ou sem inoculação (Azospirillum brasilense). A semeadura da soja foi realizada em semeadura direta em 16 de novembro de 2012 e 01 de dezembro de 2013, utilizando-se a cultivar BMX Potência RR. Em cada parcela, se utilizou sete fileiras de plantas com espaçamento de 0,45 m, sendo que em quatro fileiras as sementes foram inoculadas com a bactéria Azospirillum brasilense. Foram avaliadas variáveis fitomorfológicas e a produtividade de grãos. Em sistema de integração lavoura-pecuária a cultura da soja possui melhores produtividades quando estabelecida sobre pastagem de aveia preta e azevém manejadas com alturas de pastejo entre 20 e 30 cm. As áreas sob pastejo convencional no inverno proporcionam plantas de soja menores, com menores inserções de primeira e última vagem. A inoculação com Azospirillum brasilense não deve ser usada isoladamente na cultura da soja. | ABSTRACT The objective of this study was to evaluate the phytomorphological parameters of soybean in a crop-livestock system inoculated with Azospirillum brasilense. The experiment was conducted in Santa Maria, Brazil, for two agricultural years (2 Paper extracted from the doctoral thesis of the first author. 012/2013 and 2013/2014) in a randomized complete block design with three replications. In the winter, the black oat and ryegrass pasture was managed with sheep under different grazing systems: (I) - a conventional grazing (CG) system, where the animals remained in the pasture throughout the experimental period without any pasture height control; (II, III, and IV) - systems with post-grazing pasture heights of 10, 20, and 30 cm, respectively; and (NG) - one control without grazing. All treatments received two nitrogen doses (50 and 100 kg ha-1) and inoculation or no inoculation (A. brasilense). The soybean cultivar BMX Potência RR was sown using no-tillage on 16 November 2012 and 1 December 2013. In each plot, seven rows of plants with spacing of 0.45 m were used, and in four rows, the seeds were inoculated with the bacterium A. brasilense. The phytomorphological variables and grain productivity were evaluated. In a croplivestock system, soybean has better productivity when established on black oat and ryegrass pasture managed with post-grazing pasture heights of between 20 and 30 cm. Areas under conventional grazing in the winter led to smaller soybean plants with lower first and last pod heights. Inoculation with A. brasilense should not be used alone in soybean cultivation.

The objective of this study was to evaluate the phytomorphological parameters of soybean in a crop-livestock system inoculated with Azospirillum brasilense. The experiment was conducted in Santa Maria, Brazil, for two agricultural years (2 Paper extracted from the doctoral thesis of the first author. 012/2013 and 2013/2014) in a randomized complete block design with three replications. In the winter, the black oat and ryegrass pasture was managed with sheep under different grazing systems: (I) - a conventional grazing (CG) system, where the animals remained in the pasture throughout the experimental period without any pasture height control; (II, III, and IV) - systems with post-grazing pasture heights of 10, 20, and 30 cm, respectively; and (NG) - one control without grazing. All treatments received two nitrogen doses (50 and 100 kg ha-1) and inoculation or no inoculation (A. brasilense). The soybean cultivar BMX Potência RR was sown using no-tillage on 16 November 2012 and 1 December 2013. In each plot, seven rows of plants with spacing of 0.45 m were used, and in four rows, the seeds were inoculated with the bacterium A. brasilense. The phytomorphological variables and grain productivity were evaluated. In a croplivestock system, soybean has better productivity when established on black oat and ryegrass pasture managed with post-grazing pasture heights of between 20 and 30 cm. Areas under conventional grazing in the winter led to smaller soybean plants with lower first and last pod heights. Inoculation with A. brasilense should not be used alone in soybean cultivation.

ABSTRACT Some forages release allelopathic substances into the environment, and may prevent consortium with arboreal species in pastures. The objective of this work was to evaluate photosynthetic characteristics of pequizeiro plants (hereafter pequi) influenced by concentrations of residues of the forage species Urocholoa decumbens, Melinis minutiflora and Paspalum notatum. The treatments consisted of pequi cultivation under aerial residues of the three forage species mixed to the substrate in four concentrations (1, 2, 3 and 4% mass/mass), plus an additional treatment (comparative control) with pequi cultivated on the substrate without waste. The following parameters were evaluated: photosynthetic rate, stomatal conductance, transpiration rate, relationship between internal and external CO2 concentration (Ci/Ca), maximum quantum yield, effective quantum yield of FS II, electron transport rate, nonchemical quenching and chlorophyll index, concerning the content of chlorophyll a, b and total in pequi plants at 50 and 100 days after transplanting (DAT), and the relative production of dry matter at 100 DAT. At 50 DAT, the following photosynthetic variables were affected in pequi plants: A, gs, chlorophyll a, chlorophyll b and total chlorophyll when cultivated in the presence of forage residues. At 100 DAT, the following photosynthetic variables were affected in pequi plants: A, Fv/Fm, ETR, NPQ, chlorophyll a, chlorophyll b and total chlorophyll, when cultivated in the presence of forage residues. Pequi plants had reduced relative dry matter yield when grown in the presence of U. decumbens. This variable was also affected when pequi was grown in increasing concentrations of residues of the species U. decumbens, M. minutiflora and P. notatum. | RESUMO Algumas forrageias liberam no ambiente substancias alelopáticas, podendo inviabilizar o consórcio com espécies arbóreas em pastagens. O objetivo deste trabalho foi avaliar características fotossintéticas do pequizeiro influenciadas por concentrações de resíduos das espécies forrageiras Urocholoa decumbens, Melinis minutiflora e Paspalum notatum. Os tratamentos consistiram do cultivo de pequi sob resíduos da parte aérea das três espécies forrageiras misturadas ao substrato em quatro concentrações (1, 2, 3 e 4% massa/massa), mais um tratamento adicional (testemunha comparativa) com pequi cultivado no substrato sem resíduos. Foi avaliada a taxa fotossintética, condutância estomática, taxa transpiratória, relação entre a concentração interna e externa de CO2 (Ci/CA), rendimento quântico máximo, rendimento quântico efetivo do FS II, taxa de transporte de elétrons, quenching não fotoquímico e índice clorofilog referente ao conteúdo das clorofilas a, b e total em plantas de pequi aos 50 e 100 dias após o transplantio (DAT) e a produção relativa referente à matéria seca aos 100 DAT. Aos 50 DAT, as plantas de pequi foram afetadas quanto as variáveis fotossintéticas A, g s , clorofila a, clorofila b e clorofila total, quando cultivadas na presença de resíduos das forrageiras. Aos 100 DAT, as plantas de pequi foram afetadas quanto as variáveis fotossintéticas A, Fv/Fm, ETR, NPQ, clorofila a, clorofila b e clorofila total, quando cultivadas na presença de resíduos das forrageiras. O pequizeiro tem produção de matéria seca relativa reduzida quando cultivado na presença de resíduos da espécie U. decumbens. Essa variável também é afetada quando o pequi é cultivado em concentrações crescentes de resíduos das espécies U. decumbens, M. minutiflora e P. notatum.

Some forages release allelopathic substances into the environment, and may prevent consortium with arboreal species in pastures. The objective of this work was to evaluate photosynthetic characteristics of pequizeiro plants (hereafter pequi) influenced by concentrations of residues of the forage species Urocholoa decumbens, Melinis minutiflora and Paspalum notatum. The treatments consisted of pequi cultivation under aerial residues of the three forage species mixed to the substrate in four concentrations (1, 2, 3 and 4% mass/mass), plus an additional treatment (comparative control) with pequi cultivated on the substrate without waste. The following parameters were evaluated: photosynthetic rate, stomatal conductance, transpiration rate, relationship between internal and external CO2 concentration (Ci/Ca), maximum quantum yield, effective quantum yield of FS II, electron transport rate, nonchemical quenching and chlorophyll index, concerning the content of chlorophyll a, b and total in pequi plants at 50 and 100 days after transplanting (DAT), and the relative production of dry matter at 100 DAT. At 50 DAT, the following photosynthetic variables were affected in pequi plants: A, gs, chlorophyll a, chlorophyll b and total chlorophyll when cultivated in the presence of forage residues. At 100 DAT, the following photosynthetic variables were affected in pequi plants: A, Fv/Fm, ETR, NPQ, chlorophyll a, chlorophyll b and total chlorophyll, when cultivated in the presence of forage residues. Pequi plants had reduced relative dry matter yield when grown in the presence of U. decumbens. This variable was also affected when pequi was grown in increasing concentrations of residues of the species U. decumbens, M. minutiflora and P. notatum.

RESUMO O arranjo espacial e a densidade populacional das culturas componentes quando bem estruturados podem contribuir para aumentar os rendimentos das culturas em relação ao monocultivos. Assim, o objetivo deste trabalho foi avaliar o desempenho produtivo da cenoura e do feijão-caupi em sistema consorciado sob diferentes arranjos espaciais e densidades populacionais. Essa pessquisa foi conduzida na fazenda experimental "Rafael Fernandes" da Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brasil. O delineamento experimental foi de blocos completos casualizados com quatro repetições, em esquema fatorial 3 x 4 em que o primeiro fator foi três arranjos espaciais (2: 2, 3:3 e 4:4) e o segundo fator quatro densidades populacionais de feijão-caupi (100, 80, 60 e 40% da população recomendada no cultivo solteiro - PRCS). O experimento foi adubado com flor-de-seda (Calotropis procera (Ait.) R.Br.), uma espécie espontânea do bioma Caatinga. As características avaliadas na cenoura foram: produtividade total e comercial de raízes e produtividade classificada de raízes. Para o feijão-caupi foi avaliado as seguintes características: número de vagens por metro quadrado, comprimento e produtividade de vagens verdes, número de grãos por vagem, peso de 100 grãos e produtividade de grãos verdes. A vantagem monetária corrigida foi utilizada para avaliar a viabilidade dos sistemas consorciados. Não se observou influencia dos arranjos espaciais entre as culturas componentes no desempenho agroeconômico do consórcio de cenoura x feijão-caupi. A mais alta performance agroeconômica do consórcio cenoura x feijão-caupi foi obtida na densidade populacional de caupi de 42% da PRCS. | ABSTRACT Spatial arrangement and population density of component cultures, when well structured, may contribute to increased crop yields relative to monocultures. Thus, the objective of this work was to evaluate the productive performance of carrot and cowpea in an intercropping system under different spatial arrangements and population densities. This research was conducted on the “Rafael Fernandes” experimental farm of the Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil. The experimental design was a randomized complete block with four replicates, where the treatments were arranged in a 3 × 4 factorial scheme, in which the first factor was three spatial arrangements (2:2, 3:3, and 4:4) and the second factor was four different population densities of cowpea (100%, 80%, 60%, and 40% of the recommended population in the single crop [RPSC]). Rooster tree Calotropis procera (Ait.) R.Br., a spontaneous species of the „Caatinga‟ biome, was used as fertilizer. The characteristics evaluated in carrot were as follows: total and commercial productivity of roots and classified root production. The following characteristics were evaluated for cowpea: number of pods per square meter, length and yield of green pods, number of grains per pod, 100grain weight, and yield of green grains. The modified monetary advantage was used to evaluate the feasibility of the intercropped systems. Spatial arrangements between the component cultures did not influence the agroeconomic performance of the carrot-cowpea intercropping system. The highest agro-economic performance of the carrot-cowpea intercropping was obtained in the population of cowpea, which was 42% of the RPSC.

Spatial arrangement and population density of component cultures, when well structured, may contribute to increased crop yields relative to monocultures. Thus, the objective of this work was to evaluate the productive performance of carrot and cowpea in an intercropping system under different spatial arrangements and population densities. This research was conducted on the “Rafael Fernandes” experimental farm of the Universidade Federal Rural do Semi-Árido (UFERSA), Mossoró, RN, Brazil. The experimental design was a randomized complete block with four replicates, where the treatments were arranged in a 3 × 4 factorial scheme, in which the first factor was three spatial arrangements (2:2, 3:3, and 4:4) and the second factor was four different population densities of cowpea (100%, 80%, 60%, and 40% of the recommended population in the single crop RPSC). Rooster tree Calotropis procera (Ait.) R.Br., a spontaneous species of the „Caatinga‟ biome, was used as fertilizer. The characteristics evaluated in carrot were as follows: total and commercial productivity of roots and classified root production. The following characteristics were evaluated for cowpea: number of pods per square meter, length and yield of green pods, number of grains per pod, 100grain weight, and yield of green grains. The modified monetary advantage was used to evaluate the feasibility of the intercropped systems. Spatial arrangements between the component cultures did not influence the agroeconomic performance of the carrot-cowpea intercropping system. The highest agro-economic performance of the carrot-cowpea intercropping was obtained in the population of cowpea, which was 42% of the RPSC.

ABSTRACT The low use of technologies by farmers and the occurrence of biotic and abiotic stresses are limiting factors for cowpea production in the Brazilian Northeast region. The tolerance of genotypes to drought is an alternative to decrease the negative effects of stresses on cowpea production. Thus, the objective of this work was to identify parents and combinations of cowpea genotypes with high probability of generating segregating populations with tolerance to drought. Six cowpea genotypes were crossed in a complete diallel cross design, totaling 30 F2 populations, which were evaluated together with their parents in an experiment under water deficit at the experimental field of the Embrapa Mid-North, Teresina, State of Piauí, Brazil. A triple lattice incomplete block experiment design was used, with three replications, with experimental plot consisting of six 2-m plant rows. Fifteen plants per plot were sampled to evaluate their agronomic characteristics, whose results were subjected to analysis of variance and means were used to estimate the general and specific combining abilities. The genotypes showed significant differences in all characteristics evaluated, denoting the genetic variability of the population. The additive effects were more important than the non-additive effects, and maternal inheritance was detected. The genotypes BRS Xiquexique, Pingo-de-Ouro-1-2 and MNC99-510F-16-1 were the most promising for use in recurrent selection programs for tolerance to water deficit. The hybrid combinations (1) BRS Paraguaçu X (4) CNCx-698-128G, (2) Pingo-de-Ouro-1-2 X (3) BRS Xiquexique, (3) BRS Xiquexique X (5) Santo-Inácio, (4) CNCx-698-128G X (6) MNC99-510F-16-1 and (5) Santo-Inácio X (4) CNCx-698-128G showed potential for generating superior lineages regarding bean production and tolerance to water deficit. | RESUMO A baixa adoção de tecnologias pelos agricultores e a ocorrência de estresses bióticos e abióticos são fatores que limitam a produção do feijão-caupi na região Nordeste do Brasil. A tolerância de genótipos à seca é uma das formas para diminuir os efeitos negativos dos estresses sobre a produtividade. O objetivo deste trabalho foi identificar genitores e combinações promissoras com maior probabilidade de gerarem populações segregantes de feijão-caupi tolerantes à seca. Foi realizado um dialelo completo envolvendo seis genótipos de feijão-caupi, totalizando 30 populações F2, que foram avaliadas, juntamente com seus genitores, em um ensaio sob déficit hídrico, no campo experimental da Embrapa Meio-Norte, em Teresina-PI. Utilizou-se o delineamento experimental látice triplo, com parcela de 6 linhas de 2 m, sendo amostradas 15 plantas por parcela. Foram avaliados caracteres agronômicos e submetidos à análise de variância e, utilizando-se das médias, obtiveram-se as estimativas de capacidade geral e específica de combinação. Observou-se diferenças significativas para todos os caracteres, evidenciando a existência de variabilidade genética na população. Os efeitos aditivos foram mais importantes que os efeitos não aditivos, sendo também constatada a presença de herança materna. Os genótipos BRS Xiquexique, Pingo de Ouro-1-2 e MNC99- 510F-16-1 foram os mais promissores para utilização em programa de seleção recorrente visando tolerância ao déficit hídrico. As combinações híbridas (1) BRS Paraguaçu X (4) CNCx 698-128G, (2) Pingo de Ouro-1-2 X (3) BRS Xiquexique, (3) BRS Xiquexique X (5) Santo Inácio, (4) CNCx 698-128G X (6) MNC99-510F-16-1 e (5) Santo Inácio X (4) CNCx 698-128G, possuem potencial simultâneos para extração de linhagens superiores quanto à produtividade de grãos e tolerantes ao déficit hídrico.

The low use of technologies by farmers and the occurrence of biotic and abiotic stresses are limiting factors for cowpea production in the Brazilian Northeast region. The tolerance of genotypes to drought is an alternative to decrease the negative effects of stresses on cowpea production. Thus, the objective of this work was to identify parents and combinations of cowpea genotypes with high probability of generating segregating populations with tolerance to drought. Six cowpea genotypes were crossed in a complete diallel cross design, totaling 30 F2 populations, which were evaluated together with their parents in an experiment under water deficit at the experimental field of the Embrapa Mid-North, Teresina, State of Piauí, Brazil. A triple lattice incomplete block experiment design was used, with three replications, with experimental plot consisting of six 2-m plant rows. Fifteen plants per plot were sampled to evaluate their agronomic characteristics, whose results were subjected to analysis of variance and means were used to estimate the general and specific combining abilities. The genotypes showed significant differences in all characteristics evaluated, denoting the genetic variability of the population. The additive effects were more important than the non-additive effects, and maternal inheritance was detected. The genotypes BRS Xiquexique, Pingo-de-Ouro-1-2 and MNC99-510F-16-1 were the most promising for use in recurrent selection programs for tolerance to water deficit. The hybrid combinations (1) BRS Paraguaçu X (4) CNCx-698-128G, (2) Pingo-de-Ouro-1-2 X (3) BRS Xiquexique, (3) BRS Xiquexique X (5) Santo-Inácio, (4) CNCx-698-128G X (6) MNC99-510F-16-1 and (5) Santo-Inácio X (4) CNCx-698-128G showed potential for generating superior lineages regarding bean production and tolerance to water deficit.

ABSTRACT Cowpea is one of the major food crops in Northeast Brazil, where it is commonly cultivated in the semi-arid regions with limited water availability. It is important to study the elicitors associated with cowpea to mitigate any deleterious effects of abiotic stress on the initial establishment of this crop. In this study, we aimed to evaluate the morphophysiological changes in cowpea cultivars under osmotic stress with seeds soaked in salicylic acid. The germination test was conducted in B.O.D germination chambers. The seeds of three cowpea cultivars: BRS Tumucumaque, BRS Aracê, and BRS Guariba, were germinated at five osmotic potentials (0.0; -0.2; -0.4; -0.6, and -0.8 MPa) after three pre-treatments: pre-soaking in deionized water, pre-soaking in salicylic acid, and without pre-soaking. The following parameters were evaluated: germination, germination speed index, seedling height, total phytomass, contents of chlorophyll ‘a’ and ‘b’, carotenoid content, electrolyte leakage, water content, and proline content. Our results indicate that salicylic acid promotes reduction in the harmful effects of abiotic stress, which is reflected in the increase in germination percentage, seedling height, and chlorophyll and carotenoid content as well as in the adjustment of electrolyte leakage and increase in proline content under induced water stress conditions. The cultivar BRS Guariba proved to be more tolerant to water deficit during germination and initial growth stages, when the seeds were treated with salicylic acid (1 mM). | RESUMO O feijão-caupi é uma das principais culturas alimentares no Nordeste do Brasil, onde é geralmente cultivada nas regiões semiáridas com pouca disponibilidade de água. É importante estudar os elicitores associados ao caupi para mitigar os efeitos deletérios do estresse abiótico no estabelecimento inicial desta cultura. Neste estudo, buscaram-se avaliar as alterações morfofisiológicas em cultivares de caupi sob estresse osmótico com sementes embebidas em ácido salicílico. O teste de germinação foi realizado em câmaras de germinação B.O.D. As sementes de três cultivares de caupi: BRS Tumucumaque, BRS Aracê e BRS Guariba foram germinadas em cinco potenciais osmóticos (0,0; -0,2; -0,4; -0,6 e -0,8 MPa) após três pré-tratamentos: pré-imersão em Água desionizada, pré-imersão em ácido salicílico e sem pré-imersão. Foram avaliados os seguintes parâmetros: germinação, índice de velocidade de germinação, altura da plântula, fitometria total, conteúdo de clorofila 'a' e 'b', conteúdo de carotenóides, vazamento eletrolítico, teor de água e conteúdo de prolina. Os resultados indicam que o ácido salicílico promove a redução dos efeitos nocivos do estresse abiótico, o que se reflete no aumento da porcentagem de germinação, altura da plântula e teor de clorofila e carotenóides, bem como no ajuste do vazamento eletrolítico e aumento do teor de prolina sob condições de estresse hídrico. A cultivar BRS Guariba mostrou-se mais tolerante ao déficit de água durante a germinação e estágios de crescimento inicial, quando as sementes foram tratadas com ácido salicílico (1 mM).

Cowpea is one of the major food crops in Northeast Brazil, where it is commonly cultivated in the semi-arid regions with limited water availability. It is important to study the elicitors associated with cowpea to mitigate any deleterious effects of abiotic stress on the initial establishment of this crop. In this study, we aimed to evaluate the morphophysiological changes in cowpea cultivars under osmotic stress with seeds soaked in salicylic acid. The germination test was conducted in B.O.D germination chambers. The seeds of three cowpea cultivars: BRS Tumucumaque, BRS Aracê, and BRS Guariba, were germinated at five osmotic potentials (0.0; -0.2; -0.4; -0.6, and -0.8 MPa) after three pre-treatments: pre-soaking in deionized water, pre-soaking in salicylic acid, and without pre-soaking. The following parameters were evaluated: germination, germination speed index, seedling height, total phytomass, contents of chlorophyll ‘a’ and ‘b’, carotenoid content, electrolyte leakage, water content, and proline content. Our results indicate that salicylic acid promotes reduction in the harmful effects of abiotic stress, which is reflected in the increase in germination percentage, seedling height, and chlorophyll and carotenoid content as well as in the adjustment of electrolyte leakage and increase in proline content under induced water stress conditions. The cultivar BRS Guariba proved to be more tolerant to water deficit during germination and initial growth stages, when the seeds were treated with salicylic acid (1 mM).