Aiming to study the genetic control of Papaya ringspot virus, type watermelon (PRSV-W) in watermelon, the cultivar Crimson Sweet (P1 – susceptible) and L26 derived from PI 244019 (P2 – resistant), as well as the resulting populations F1, F2, RC11 and RC21 of the cross of both lines were evaluated. The trials were carried out in a greenhouse, and the evaluations were done using artificial inoculations with PRSV-W isolates. The seedling symptoms were recorded using a graded scale, and the serological evaluation was done with specific antiserum using indirect ELISA. The estimated variances of the populations were used to obtain the genetic (σ 2 G), the environmental (σ 2 E), phenotypic (σ 2 F2), additive (σ 2 A) and dominance (σ 2 D) variances as well as the broad (h2 a) and narrow sense (h2 r) heritabilities. The hypothesis of monogenic inheritance was tested under different presumed average degrees of dominance as well as using the maximum likelihood. The distribution of resistant plants in the segregating populations was different from a distribution based on monogenic inheritance for all presumed average degrees of dominance, therefore, the hypothesis of monogenic inheritance was rejected indicating that this character in the line L26 is controlled by more than one major gene with the presence of modifiers. The additive-dominant model was adequate to explain the type of gene action involved, and the epistatic effects were not important in the expression of the resistance. The estimated average degree of dominance indicated complete dominance. The broad sense heritabilities for the two variables analyzed were intermediate.

– The objective of this study was to evaluate the effects of different water depths and nitrogen fertilization levels applied by fertigation on the physical attributes of guava fruits cv. Paluma. The study was carried out taking into account factors water depths (1144, 1465, 1785 and 2106 mm) and nitrogen fertilization levels (50, 100, 150 and 200 kg ha-1 ). The water depths 1730 and 1750 mm increased production and the average weight of guava fruit in, respectively, 25.1 and 31.6%. The applications 178 and 152 kg N ha-1 increased at 67.1% in the production of fruits and 16.3% the average fruit weight. The combined application of 150 kg N ha-1 and 1789 mm of water amounted to 63.3% of the total weight of fruit. The equatorial diameter of the fruit was influenced by individual water depths applied evidencing an increase of up to 26.8%. Already combinations 200 kg N ha-1 and 1746 mm of water increased the longitudinal diameter of the fruits by 18.77%. The application of water depth to 1144 mm, associated with 143 kg N ha-1 gave index of the most satisfactory way to guava fruit.

The objective of this Study was to evaluate the effect of organic fertilizer and cuts on the chemical composition and in vitro digestibility of the air pornunça biomass. The experimental design of randomized blocks in a split plot with four courts, two fertilization, and four replications. The cuts were made in February, June and October 2011 and February 2012. The fertilizer was with cattle and sheep manure, applied in May 2010 and March 2011 in the amount of 20 Mg ha-1 . We evaluated the structural components of the shoot, such as leaf blade, petiole, branch and full (leaf blade and petiole more branch) of pornunça. The manure beef and lamb did not influence (P>0.05) the chemical composition of the structural components of the shoot. Differences were noted between the cuts, except for dry matter (DM) of the leaf blade and the full component of ether extract (EE), neutral detergent fiber (NDF), acid detergent fiber (ADF) and hemicellulose (HC). There was a significant (P<0.05) for the in vitro digestibility of the MS of the structural components in different cuts, with the highest digestibility for structural components in the cut made in February 2011. Lower crude protein was found in the petiole and components branch. The pornunça should be considered as an alternative food for animals according to the chemical composition of the values presented in this study, with the leaf surface of the plant part that presents the best nutritional value.

Despite the knowledge that you have about the benefits of the humus of the worms or ver- micompost, there is a need to evaluate the technical feasibility of using other sources of carbon for worms. The objective was to evaluate the possibility of using straw of coffee to vermicompost production, the effects of their use on worms and on the quality of humus obtained. The evaluated treatments consisted of: (1 straw of coffee (SC) 100%; 2 SC 75% + manure (MA); 3 50% SC + 50% MA; 4 25% SC + 75% MA; 5 100% MA). The straw of coffee and the manure were incubated for 64 days to make them available to feed the worms and more 84 days to digest the substrate. The straw of coffee increased the number of worms without resulting in harmful effects. The percentage increment of the straw in the constitution of the vermicompost increased the availability of N, K, Ca and Cu, but it reduced the availability of Mg, S, Mn, P, Fe and Zn. Aiming o the medi- um supply of nutrients, the proportion of the 50% of straw of coffee and 50% of manure is designated.

This study aimed to evaluate the in vitro behavior of the fungus Fusarium guttiform in different conditions of temperature and luminosity and determine the most efficient inoculation method for evaluating the resistance of pineapple to fusariosis. To determine the in vitro behavior, two experiments was performed, with the same design, conduction, and evaluation. It was used a completely randomized experimental design in a factorial arrangement of 3x3 (three temperatures and three light regimes), with five replications. The evaluations of inoculation methods were conducted in a completely randomized design with factorial arrangement 3x4 (methods x distance of inoculation). D leaves of pineapple, cultivar Pearl, were inoculated at two, five, eight and eleven centimeters from the base by the methods of infected toothpick, mycelium disc without injury and mycelium disc with injury on the leaf. There was no significant difference on in vitro behavior of the isolates analyzed. The indicated temperature and luminosity for the multiplication of F. guttiform were 25°C and 12 hours, respectively, since they showed significant my celial growth and increased production of reproductive structures. The indicated method for evaluation of resistance to fusariosis in pineapple was the inoculation by infected toothpick at a distance between 2 - 11 cm from the base of the leaf. It becomes necessary to perform an injury at the site to be inoculated for the infection of the pathogen F. guttiform occurs.

The mechanical harvesting causes injuries on seeds and may affect their quality. Different threshing mechanisms and their adjustments may also affect the intensity of impacts that machines cause on seeds. So, this study aimed at diagnosing and evaluating the effect of two combines: the first one with a threshing system of axial flow and the other one with a threshing system of tangential flow, under adjustments of concave opening (10 mm, 30 mm and 10 mm for a combine with axial flow and 3.0 mm, 15 mm and 3.0 mm for a combine with tangential flow) and three cylinder rotations on the quality of soybean seeds harvested at two moisture contents. Soybean seeds of cultivar 'ND 4910' were harvested at 16.6% moisture (mid - morning) and 13.7% moisture in the afternoon. The seeds quality was evaluated by germination tests, germination speed index (GSI), germination rate, moisture content, percentage of purity and vigor by tetrazolium test. Despite the combine, the results showed that the mechanical injury has most reduced seeds quality, at 16.6% moisture content, concave opening of 30 mm (axial) and 10 mm (tangential) and cylinder rotation of 1100 rpm (axial) and 1000 (tangential), both with the highest rotations used. The combine with tangential flow had the highest degree of seeds purity. When seeds moisture content at harvest was close to 13.7%, there was the highest seed injury, while, at 16.6%, there was the highest number of crushed soybeans, regardless the combine adjustment.

This study sought to estimate (co)variance and genetic parameters for birth weight (BWT) and weaning weight (WWT) in Santa Ines sheep. A total of 2,111 records were obtained from EMBRAPA/CPATC experimental herds, dating from the years 1998 to 2008. (Co)variance parameters were obtained through a two - trait analysis with the Gibbs sampling algorithm using the MTGSAM program. The mixed model included the environmental effects of sex, contemporary group and type of birth, in addition to residual, direct and maternal additive effects. Mean estimates of direct heritability for BWT and WWT were 0.25 and 0.09, respectively. Mean estimates of maternal heritability were 0.34 for BWT and 0.24 for WWT. The genetic correlation be- tween BWT and WWT was 0.14. The results suggest that breeding Santa Ines sheep for meat production must take into consideration direct and maternal additive genetic effects.

– The downy mildew, caused by the fungus Pseudoperonospora cubensis is a major foliar disease that attacks the melon crop in the rainy season in northeastern Brazil. The objective of this study was to identify and characterize morphologically resistant melon accessions to P. cubensis. Thirty-six accessions and four commercial cultivars were evaluated in a randomized block design with three replications. The plot was composed of seven plants. The evaluation was conducted in the under field conditions with a diagrammatic scale and calculating the disease percentage index (DPI). Resistance sources and references genotypes were characterized morphologically. The accessions C-RN-2, C-SE-2, C-CE-2, C-PE-2, C-PI-1, and C-BA-2 show resistance to P. cubensis and are promising for use in programs breeding for resistance to downy mildew although its fruits have reduced commercial quality.

The objective was to evaluate the growth of clones of forage cactus, subjected to different conditions of water availability in the Brazilian Semiarid, during a year of climate anomaly (biennium 2012-2013). The experiment was carried out in the first production year of the second crop cycle (March 2012 to February 2013), in the district of Serra Talhada, State of Pernambuco. The experiment was carried out in a randomized block design using a factorial arrangement 3x3 with three replications, being used three intervals of application of a fixed irrigation depth (7.5 mm), (7, 14 and 28 days), and three forage cactus clones (IPA: IPA Sertânia; MIU: Míuda and, OEM: Orelha de Elefante Mexicana). Eight biometric campaigns were performed during the experiment period for morphological analysis of the plants and cladodes along the time. It was found that water availability conditions showed no significant influence (P>0.05) on the majority of the absolute and relative values of the growth variables of the three forage cactus clones. However, when comparisons were performed among different clones, regardless of the water availability conditions, it was observed that, in terms of absolute values, the OEM had the highest means, differing only from the MIU clone. Regarding evaluations over time, there were increased growth rates in the last months of the crop cycle because of the occurrence of rainfall, which in conjunction with the application of the irrigation treatments promoted the best biometric increments for the OEM and IPA Sertânia clones.

This study aimed to evaluate the culture of Litopenaeus vannamei marine shrimp fed with dif- ferent protein levels diets in heterotrophic systems with and without probiotic addition. It was adopted a com- pletely randomized design with 4x2 factorial arrangement, using four dietary protein levels (20, 25, 30 e 35% CP), as the first factor (P20, P25, P30, and P35), and probiotic addition in the water, as the second factor (P20 Pro , P25 Pro P30 Pro e P35 Pro ). For this were used 24 fiberglass tanks (800 L working volume) stocked with 300 shrimp m - 3 (initial weight 1.55±0.01 g). Water quality parameters were analyzed periodically and showed no significant differences, except nitrite that was influenced by the protein levels ( P<0.05 ). After 50 culture days, shrimp final weight averaged 7.2±0.4 g ( P≥0.05 ). The interaction of protein levels vs. probiotic addition influenced significantly ( P<0.05 ) the survival (70.6 to 90.0%) and final biomass (1.3 - 2.0 Kg m - 3 ). In Litopenaeus vannamei intensive culture with the utilization of biofloc as the supplemental food, it is possible to reduce the protein levels of feed from 35 to 25%, without compromising the shrimp growth performance and water quality of the culture.