ABSTRACT Water deficit is a current problem of global relevance. This is a problem in Brazil, especially in the Semiarid region, which undergoes severe droughts for much of the year. This requires the search for alternatives, such as the planned wastewater use, which is a successful alternative in developed countries that is applied for various purposes, including agriculture. However, despite the increasing growth of agriculture in Brazil, there are no established control and monitoring policies to minimize its impacts on the environment and human health. The country does not have specific legislation that defines wastewater quality parameters for domestic sewage. The objective of this work was to compile international guidelines and legislations that address this issue and compare them with the national normative resolutions for quality of wastewater and its different uses. The most relevant and locally applicable parameters described in these documents were listed, and the reference limits were defined by comparing them with those found in the scientific literature applied to the Brazilian context. This compilation represents the first step towards the incorporation of regulations for water reuse, having the Brazilian Semiarid region as a first test field, but with expandable application to different regions of the country. | RESUMO O déficit hídrico é uma problemática atual de relevância mundial. No Brasil, em especial no semiárido, essa realidade alcança níveis elevados de seca em boa parte do ano, sendo necessária a busca de alternativas mitigadoras, como o reúso planejado de águas residuárias, alternativa instaurada com sucesso nos países desenvolvidos, aplicado para diversos fins, inclusive na agricultura. No cenário nacional, mesmo diante do acentuado crescimento dessa atividade, não existem, políticas definidas de controle e monitoramento que visam minimizar impactos causados ao meio ambiente e à saúde humana. Ademais, o país não dispõe de legislação específica que contemple parâmetros da qualidade de água de reúso proveniente de esgoto doméstico. Nesse sentido, este trabalho teve como objetivo analisar as diretrizes e legislações internacionais que tratam dessa temática associadas às normativas nacionais que discorrem sobre a qualidade da água de reúso em suas diversas aplicações. Foram elencados os parâmetros de maior relevância e aplicabilidade local, descritos nesses documentos, e os limites de referência definidos a partir da comparação com a bibliografia científica aplicada ao contexto brasileiro. Essa compilação representa o primeiro passo para uma futura incorporação regulamentar da atividade de reúso de águas, tendo como campo primário de aplicação o semiárido, mas expansível às diversas regiões do país.

ABSTRACT The objective was to evaluate the production, chemical composition and hygienic -sanitary parameters of the milk of Murrah buffaloes raised in the northeastern semi-arid region. Data from 13,752 observations of lactations collected from the years of 2013 to 2016 from a herd in Rio Grande do Norte were used. The milk quality through chemical composition (contents of fat, protein, lactose, and total solids), somatic cell counts (CCS), and milk production was evaluated. Data were submitted to Pearson correlation, factorial analysis, multivariate analysis of variance per year, and per period (dry, rainy and transition) and canonical discriminant analysis by year and period. A high and positive correlation was observed for total solids and fat (0.91) and positive correlation for protein and total solids, yield, and lactose. Through factor analysis, four factors were selected that explained 88% of the total variation. The first factor was considered "milk quality factor, " the second factor as "milk production factor," and the third factor as " sanity factor." There was significant effect based on year but not significant effect based on period for the studied variables. In the canonical discriminant analysis per year, the discriminant power variables were (CCS), milk production and total solids. For periods were protein, production, lactose, fat, and total solids. Multivariate analysis was efficient in evaluating the production and chemical composition of buffalo milk and can be safely used in future studies. | RESUMO Objetivou-se avaliar a produção, composição química e parâmetro de qualidade higiênico sanitária do leite de búfalas da raça Murrah criadas no semiárido nordestino. Foram utilizados dados de 13.752 observações de lactações coletados dos anos de 2013 a 2016 em plantel no Rio Grande do Norte. Foram avaliadas a qualidade do leite através da composição química (teores de gordura, proteína, lactose, e sólidos totais), contagem de células somáticas (CCS) e produção do leite. Os dados foram submetidos a correlação de Pearson, análise fatorial, análise de variância multivariada por ano e por período (seco, chuvoso e transição) e análise discriminante canônica por ano e por período. Correlação alta e positiva foi observada para sólidos totais e gordura (0,91), e correlação positiva para proteína e sólidos totais, produção e lactose. Por meio da análise fatorial, foram selecionados 3 fatores que explicaram 88% da variação total. O primeiro fator foi considerado "fator qualidade do leite", o segundo fator como "fator produção de leite" e o terceiro como "fator sanidade". Houve efeito significativo de ano e não significativo de períodos para as variáveis estudadas. Na análise discriminante canônica por ano as variáveis de poder maior discriminante foram (CCS), produção de leite e sólidos totais. Dentro de período, as variáveis mais discriminantes foram proteína, produção, lactose, gordura e sólidos totais. As análises multivariadas mostram-se eficiente na avaliação da produção e composição química do leite búfala, podendo ser usada com segurança em futuros estudos dessa natureza.

RESUMO A umbu-cajazeira (Spondias sp.), frutífera nativa do Nordeste brasileiro, produz frutos muito valorizados pelas agroindústrias. O objetivo deste trabalho foi avaliar a aplicação de agroquímicos e lesões no enraizamento e na formação de mudas/estacas, foi conduzido na Embrapa em Pacajus, CE, em blocos ao acaso, com cinco tratamentos: T1 = testemunha, T2 = estacas feridas + Sela Gel®, T3 = estacas feridas + Radimax®, T4 = estacas incisadas + Sela Gel® e T5 = estacas incisadas + Radimax®. Os ambientes (blocos): pleno sol; teto com plena sombra; sombrite 50% nas laterais e no teto; e sombrite 50% no teto. As parcelas tinham oito estacas, totalizando 160 estacas. Dos propágulos retirados de plantas, preparam-se estacas lenhosas, com 25 cm de comprimento e médias de 29,7 mm de diâmetro e 8,7 gemas/estaca. Em seguida foram tratadas com solução de hipoclorito de sódio a 0,05%/4 minutos; depois fez-se os ferimentos e as incisões e aplicou-se o Sela Gel® por pincelamento e o Radimax® por imersão em solução de 10 mL/L d'água/10 minutos. O plantio foi em vasos plásticos pretos, com capacidade 6L, cheios com vermiculita + pó de cascas de coco + bagana de carnaúba triturada + cascas de arroz carbonizada (2:1:1:1, v/v), previamente pasteurizados. Entre os tratamentos e os blocos houve diferenças significativas. A incisão e a aplicação do Sela Gel® induziram a formação de calo, o enraizamento e a formação de mudas vigorosas, enquanto o Radimax® não induziu o enraizamento. | ABSTRACT Umbu-caja (Spondias sp.) is a fruit tree native to the Northeast region of Brazil, which is grown because of its highly valuable fruits for fruit processing industries. The objective of this work was to evaluate the effect of agrochemical applications and cutting types on the rooting and development of Spondias sp. plantlets. The experiment was conducted at the experimental field of the Embrapa Tropical Agroindustry, in Pacajus, CE, Brazil. A randomize block experimental design was used, with five treatments - control (T1); cuttings with two lesions at the base (CL) + rooting gel (RG) (Sela Gel®) (T2); CL + rooting fertilizer (RF) (Radimax®) (T3); cuttings with two transversal cross sections at the base (CS) + RG (T4); and CS + RF (T5). The environments (blocks) used were: full sun; 100% shade ceiling; 50% shade walls and ceiling; and 50% shade ceiling. The plots consisted of eight cuttings, totaling 160 cuttings. Propagules were taken from Spondias sp. plants to make woody cuttings with length of 25 cm, diameter of 29.7 mm, and mean bud per cutting of 8.7. All cuttings were immediately treated with a 0.05% sodium hypochlorite solution for four minutes before making the lesions and transversal cross sections and applying the treatments. The RG was applied by brushstroke, and the RF was applied by immersion in an aqueous solution of 10 mL L-1 for 10 minutes. The cuttings were planted into 6-liter black plastic pots filled with a pasteurized mixture of vermiculite, coconut husk powder, ground leaf fibers of Copernicia prunifera, and carbonized rice husk (2:1:1:1; v v-1). The treatments and blocks significantly affected the rooting and development of cuttings. The treatment with CS and application of the RG (T4) promoted root callus formation, rooting, and vigorous development of plantlets, whereas the RF (T3 and T5) failed to promote root formation.

RESUMO O cultivo do maracujazeiro-azedo Passiflora edulis (Sims, 1818) vem obtendo destaque na fruticultura nacional devido ao aumento do consumo tanto da fruta fresca, como processada. Contudo, perdas pelo ataque de percevejos (Hemiptera: Coreidae) vêm preocupando fruticultores da região sul do Brasil. O objetivo deste estudo foi avaliar a ocorrência e o controle de percevejos em pomar de maracujazeiro-azedo P. edulis, através da utilização de inseticidas naturais e sintéticos nas safras de 2013/14 e 2014/15 em Araquari/SC. A ocorrência de percevejos foi estudada através de amostragens utilizando a técnica do quadro de madeira com dimensão de 0,25 m2. As avaliações foram realizadas semanalmente durante as duas safras. As espécies e a distribuição dos percevejos nas diferentes partes da planta foram quantificadas. Os inseticidas utilizados foram: Neenmax (Azadiractina 1,0%) na dose de 10 mL/L; Orobor N1 (Ácido cítrico N 1,0% + B 0,20%) 2 mL/L; Decis 25 EC (Deltametrina 2,5%) 1 mL/L, diluídos em solução aquosa. Foram observados exemplares de três espécies de percevejos, sendo 86% Diactor bilineatus (Hemiptera: Coreidae); 13,5% Holhymenia histrio (Hemiptera: Coreidae) e 0,5% de Nezara viridula (Hemiptera: Pentatomidae). D. bilineatus apresentou alta frequência, constância e dominância durante as safras avaliadas. Os percevejos encontravam-se 64% sobre os frutos, 18,5% sobre as folhas, 7,3% sobre os botões florais, 5,8% sobre as flores e 4,4 % sobre os ramos. A pulverização com Azadiractina e Deltametrina foram eficientes para reduzir a infestação de percevejos e ocorrência de frutos murchos no pomar de maracujazeiro-azedo. | ABSTRACT Sour passion fruit (Passiflora edulis Sims, 1818) crops in Brazil are drawing attention due to the national production, and the increasing consumption of this fruit as fresh and processed products. However, fruit losses due to damages caused by stink bugs (Hemiptera: Coreidae) are concerning to fruit growers in southern Brazil. The objective of this study was to evaluate the population fluctuation and control of stink bugs in P. edulis crops grown in the 2013-2014 and 2014-2015 crop seasons in Araquari, SC, Brazil, using natural and synthetic insecticides. The stink bug population was evaluated using the 0.25 m2 frame technique. The evaluations were carried out weekly during the two crop seasons. The species and distribution of stink bugs in the different parts of the plant were evaluated. The insecticides and rates used were: Neenmax (azadirachtin 1.0%) at 10 mL L-1; Orobor N1 (N-citric acid 1.0% + 0.20% B) at 2 mL L-1; and Decis 25 EC (deltamethrin 2.5%) at 1 mL L-1, diluted in aqueous solution. Specimens of three stink bug species where found: 86% Diactor bilineatus (Hemiptera: Coreidae); 13.5% Holhymenia histrio (Hemiptera: Coreidae), and 0.5% Nezara viridula (Hemiptera: Pentatomidae). The D. bilineatus species presented higher frequency, constancy, and dominance during the evaluated crop seasons; 64% of the stink bugs were found on fruits, 18.5% on leaves, 7.3% on floral buds, 5.8% on flowers, and 4.4% on branches. The use of azadirachtin or deltamethrin was efficient to reduce the stink bug infestation and the number of P. edulis withered fruits.

Sunflower can be an economically viable crop in the Northeast region of Brazil depending on the use of appropriate irrigation and fertilization managements. The objective of this work was to evaluate production components of sunflower plants (Helianthus annuus L.) of the BRS-324 cultivar subjected to different organic fertilizer rates (goat manure) and irrigation water depths in two crop cycles (November 2014 to February 2015; and August to November 2015). The experiment was conducted in a randomized block design with a split-split-plot arrangement, consisting of five organic fertilizer rates (OFR) (0, 300, 600, 900, and 1.200 mL plant-1 week-1) and five irrigation water depths (IWD) (33; 66; 100; 133, and 166% of the class A tank evaporation), with three blocks. The highest achene yield (1,220.78 kg ha-1) in the first cycle was found using OFR of 1,200 mL plant-1 week-1 and IWD of 134.9% (524.9 mm); and the highest yield (882.07 kg ha-1) in the second crop cycle was found using the highest OFR combined with IWD of 166% (843.0 mm). The use of goat manure as organic fertilizer had no effect on the sunflower oil content; however, this variable was affected by the irrigation water depths used. Protein content was higher in the second crop cycle (14%) when using IWD of 100% and OFR of 536 mL plant-1 week-1.

The accumulation and transformation of organic matter in the soil are fundamental for the maintenance and improvement of the chemical, physical, and biological attributes of the soil, and consequently, for the tropical forests functioning. The objective of this study was to evaluate the influence of secondary forests with different successional stages on soil organic matter, carbon and nitrogen stocks, and soil fertility. Three areas of seasonal semideciduous forest, located in Pinheiral - RJ, with different successional stages were selected: initial stage forest - ISF, medium stage forest - MSF, and advanced stage forest - ASF. The values of total carbon and nitrogen, C and N stocks, δ13C (‰) isotope, chemical and granulometric fractionation of soil organic matter, and soil fertility were determined. Based on the values of δ 13C (‰), it was verified that most of the soil carbon of the different successional stages comes from forest species (C3 plants). The areas with the highest succession time (MSF and ASF) possess higher levels of carbon and nitrogen contents and stocks, and carbon associated with minerals compared to ISF. In addition, ASF showed increased phosphorus, fulvic acid, humic acid, whereas soil density exhibited reduced values when compared to that of other areas. The MSF showed, in general, greater fertility of the soil. This study emphasizes the importance of secondary forests that, even in the initial stages of succession, have great potential to store and stabilize organic carbon in the soil.

Monosporascus root rot and vine decline (MRRVD) is an important disease in the melon crop in Brazil. In our research, we evaluated the reaction of 45 melon genotypes to M. cannonballus in two successive cropping seasons in a greenhouse. Melon seedlings were transplanted into naturally infested soil. The evaluation of the genotypes was performed after 55 days, using a score scale to group each genotype into one of five classes and to calculate the disease severity index. None of the genotypes showed an immune reaction to M. cannonballus, regardless of the season. In the first cycle, only 28.9% ('AF 464', 'Auraprince', 'DRG 2278', 'Estoril', 'Guaporé', 'Kousto', 'Mabel', 'Magisto', 'Massaï', 'Nectar', '8530', 'RZ 34130' and 'RZ 34292') had a similar reaction to the high resistance to the MRRVD. Most genotypes (66.7%) behaved as moderately resistant while 4.4% were susceptible and none were highly susceptible. Conversely, in the second cycle, no genotype showed a reaction similar to high resistance while 42.2% behaved as moderately resistant, 48.9% as susceptible, and 8.9% as highly susceptible. The severity levels ranged from 7.5 to 60% and 27.5 to 92.5% for the first and second cycles, respectively. The genotypes 'DRG 2278', 'Estoril', 'Guaporé', 'Kousto', 'Mabel', 'Massaï', 'RZ 34130' and 'RZ 34292' were identified as highly resistant and moderately resistant in the first and second cycles of cultivation, respectively. Therefore, these genotypes are promising sources of resistance to M. cannonballus and should be preferred in commercial fields infested with the pathogen.

Nitrogen fertilization is a limiting factor for grapevine production; its excess or deficiency can cause changes in fruit quantity and quality. Thus, the objective of this work was to evaluate productive parameters of grapevines of the Isabel-Precoce cultivar subjected to nitrogen and organic fertilization in the western semiarid region of the state of Rio Grande do Norte (RN), Brazil. The experiment was conducted at the Experimental Farm of the Federal Rural University of the Semi-Arid Region, in Mossoró, RN, Brazil, during two production cycles. The soil of the area used was classified as dystrophic Red Argissolo (Udult) of sandy texture. The experiment was conducted in a randomized block design with six replications, using a 5×2 factorial arrangement, with 5 nitrogen fertilizer rates (0, 30, 60, 90, and 120 kg ha-1) and two organic fertilizer rates (0, and 20 m3 ha-1 of bovine manure). The production components number of bunches per plant, grape production per plant, grape yield, weight, length and width of bunches, number of berries per bunch, and ten-berry weight were evaluated. The nitrogen rates and organic fertilizer rates used were significant (p=0.05) for all evaluated components. The N rate of 60 kg ha-1 combined with the organic fertilization generated the best results for the analyzed variables. N rates from 60 to 120 kg ha-1, applied singly or combined with organic fertilizer, hindered the production components of the Isabel-Precoce grapevines.

RESUMO Considerável número de doenças que acometem a melancia (Citrullus lanatus) são causadas por patógenos habitantes do solo no Brasil e no mundo. O objetivo do presente trabalho foi identificar e quantificar a frequência de fungos fitopatógenos associados a "podridão de raízes e declínio de ramas" em melancia, presentes nas raízes de plantas daninhas nas principais áreas produtoras no estado do Rio Grande do Norte. Foram coletadas 10 amostras de raízes de espécies de plantas daninhas de maior prevalência em 16 áreas produtoras de melancia. As plantas foram identificadas e, calculada a respectiva frequência em campo. Foi realizado o isolamento fúngico das raízes, sendo posteriormente identificados os principais fungos associados ao declínio de ramas. Foram identificadas 13 espécies de plantas daninhas, pertencentes a nove famílias botânicas. As espécies de plantas daninhas que apresentaram as maiores frequências em campo foram Amaranthus spinosus (25,0%), Trianthema portulacastrum (18,8%), Commelina sp. (18,8%) e Boerhavia diffusa (12,5%). Das raízes das plantas daninhas que apresentaram maior frequência foram isolados os gêneros Macrophomina, Rhizoctonia e Monosporascus. O primeiro foi isolado de 12 espécies de plantas, o segundo de quatro espécies e o terceiro de duas espécies. | ABSTRACT Many watermelon (Citrullus lanatus) diseases are caused by soilborne pathogens in Brazil and worldwide. The goal of this study was to identify and quantify the frequency of phytopathogenic fungi associated with watermelon root rot and vine decline that were also present in the roots of weeds in the major watermelon production regions in the state of Rio Grande do Norte, Brazil. We collected root samples from 10 of the most prevalent weed species in 16 watermelon producing areas. The plants were identified and their frequencies in the fields were calculated. The fungi found in the weed roots were isolated and the main genera associated with watermelon vine decline were identified. We identified 13 weed species belonging to nine botanical families. The weed species with the highest frequencies found in the field were Amaranthus spinosus (25.0%), Trianthema portulacastrum (18.8%), Commelina sp. (18.8%), and Boerhavia diffusa (12.5%). The fungi Macrophomina, Rhizoctonia, and Monosporascus were isolated from the roots of the weed plants. While Macrophomina was isolated from 12 different types of plants, Rhizoctonia and Monosporascus were isolated from four and two different plant species, respectively.