The use of landfill leachate (LL) to produce biomass for energy and biofuel purposes is an alternative that minimizes environmental degradation. In this context, the objective of this work was to evaluate the effect of using different rates of LL in the fertigation of sunflower (Helianthus annuus L.) crops. The experiment was conducted in a randomized block design with five treatments and four replications-20 experimental plots. The treatments consisted of control with 100% water from the water supply (WWS) (T1); 80% WWS + 20% LL (T2); 60% WWS + 40% LL (T3); 40% WWS + 60% LL (T4); and 20% WWS + 80% LL (T5). The sunflower crops were grown in a eutrophic Red-Yellow Argisol for 81 days, in Mossoró RN, Brazil. The plant characteristics evaluated-plant height, number of leaves, steam diameter and capitulum diameter-was determined. All variables evaluated presented statistical differences between treatments. In general, plants in the treatment 60% WWS + 40% LL presented better performance regarding the characteristics evaluated.

RESUMO O uso do percolado de aterro sanitário (PATS) na produção de biomassa para fins energético e biocombustível é uma alternativa que minimiza a degradação ambiental. Neste contexto, objetivou-se neste trabalho, analisar o efeito da aplicação de distintas proporções de percolado de aterros sanitários no cultivo de girassol (Helianthus annuus L.). O experimento foi conduzido com delineamento em blocos casualizados, com cinco tratamentos T1 - 100 % de água da rede de abastecimento - AA (Testemunha); T2 - 80 % de AA e 20 % de PATS; T3 - 60 % de AA e 40 % de PATS; T4 - 40 % de AA e 60 % de PATS; e, T5 - 20 % de AA e 80 % de PATS, e quatro repetições, totalizando 20 parcelas experimentais. O girassol foi cultivado em ARGISSOLO Vermelho - Amarelo eutrófico, durante 81 dias em Mossoró/RN. Durante o ciclo do girassol foram determinadas as características vegetativas altura de planta (AP), número de folhas (NF), diâmetro do caule (DCaule) e do capítulo (DCapítulo). Comprovou-se que para todas as variáveis estudadas houve diferença estatística entre os tratamentos. Em geral, o tratamento 60 % de AA e 40 % de PATS apresentou melhor desempenho com relação às características vegetativas do girassol. | ABSTRACT The use of landfill leachate (LL) to produce biomass for energy and biofuel purposes is an alternative that minimizes environmental degradation. In this context, the objective of this work was to evaluate the effect of using different rates of LL in the fertigation of sunflower (Helianthus annuus L.) crops. The experiment was conducted in a randomized block design with five treatments and four replications-20 experimental plots. The treatments consisted of control with 100% water from the water supply (WWS) (T1); 80% WWS + 20% LL (T2); 60% WWS + 40% LL (T3); 40% WWS + 60% LL (T4); and 20% WWS + 80% LL (T5). The sunflower crops were grown in a eutrophic Red-Yellow Argisol for 81 days, in Mossoró RN, Brazil. The plant characteristics evaluated-plant height, number of leaves, steam diameter and capitulum diameter-was determined. All variables evaluated presented statistical differences between treatments. In general, plants in the treatment 60% WWS + 40% LL presented better performance regarding the characteristics evaluated.

The aim of this study was to evaluate the effect of the root of vetiver grass (Chrysopogon zizanioides (L.) Robert) on the soil Resistance to penetration (RP) in the margins of the lower São Francisco River. Vetiver grass seedlings were planted on the riverbank margins in order to increase soil resistance to erosion processes, and RP assessments were made at soil depths of 0-0.20, 0.20-0.40, and 0.40-0.60 m in different situations (near the plants and within rows). RP data was obtained using an automated penetrometer. Gravimetric moisture data was also collected, as well as particle size data by densimetry, and root density data by the monolith method. The results of the RP, moisture, and root density studies were subjected to analysis of variance (p <0.05) and then the averages were compared by the Tukey test using SISVAR software. The samples next to the vetiver grass showed an average RP of 1793.94 kPa and an average moisture content of 11.78%, statistically differing from that of the sampled points within rows. RP and soil moisture did not differ statistically at the depth assessed. The vetiver grass produced an adequate ground cover, leading to higher water retention and hence a reduced resistance to penetration.

ABSTRACT The aim of this study was to evaluate the effect of the root of vetiver grass (Chrysopogon zizanioides (L.) Robert) on the soil Resistance to penetration (RP) in the margins of the lower São Francisco River. Vetiver grass seedlings were planted on the riverbank margins in order to increase soil resistance to erosion processes, and RP assessments were made at soil depths of 0-0.20, 0.20-0.40, and 0.40-0.60 m in different situations (near the plants and within rows). RP data was obtained using an automated penetrometer. Gravimetric moisture data was also collected, as well as particle size data by densimetry, and root density data by the monolith method. The results of the RP, moisture, and root density studies were subjected to analysis of variance (p <0.05) and then the averages were compared by the Tukey test using SISVAR software. The samples next to the vetiver grass showed an average RP of 1793.94 kPa and an average moisture content of 11.78%, statistically differing from that of the sampled points within rows. RP and soil moisture did not differ statistically at the depth assessed. The vetiver grass produced an adequate ground cover, leading to higher water retention and hence a reduced resistance to penetration. | RESUMO O objetivo deste estudo foi avaliar o efeito da raiz de capim vetiver (Chrysopogon zizanioides (L.) Robert) sobre a resistência à penetração (RP) das margens do Baixo São Francisco. Mudas de vetiver foram plantadas no talude do rio, a fim de aumentar a resistência do solo aos processos erosivos, com avaliações de Resistência à Penetração (RP) realizadas em profundidades de 0 a 0,20, 0,20 a 0,40, e 0,40-0,60 m em diferentes situações (próximo à planta e, nas entre linhas). Dados de RP foram obtidos utilizando um penetrômetro automatizado. Amostras de solo foram coletadas para a determinação da umidade assim como da textura do solo, que foi determinada por densimetria, a densidade das raízes pelo método monólito. Os resultados do RP, umidade, e estudos de densidade de raiz foram submetidos a análise de variância (p <0,05) e, em seguida, as médias foram comparadas pelo teste de Tukey utilizando o software SISVAR. As amostras próximas ao vetiver apresentaram uma RP média de 1793,94 kPa e umidade média de 11,78%, diferindo estatisticamente dos pontos amostrados nas entrelinhas. RP e umidade do solo não diferiram estatisticamente com a profundidade do solo. O capim vetiver produziu uma cobertura do solo adequada, levando a uma maior retenção de água e, consequentemente, uma redução da resistência à penetração.

Brackish waters represent great potential for profitable agricultural production; however, productive usage depends on the adoption of proper cultural practices as well as a culture tolerant of salinity, which can require some restrictions related to soil and crop production. Given the lack of information pertaining to hydroponic melon culture, the objective of this study was to investigate physiological changes promoted by the use of brackish water in the preparation of the nutrient solution for melon (Cucumis melo L., cv. AF 015) growth in coconut fiber substrate under greenhouse conditions in Mossoró-RN, a semiarid region of Brazil. The experimental design was completely randomized, with 12 treatments arranged in a 4 x 3 factorial scheme, with 4 salt concentration levels (1.1 - control, 2.5, 4.0 and 5.5 dS m-1) and 3 exposition times (vegetative growth: 10-30 days after transplanting, DAT; flowering: 31 to 50 DAT; and fruiting and ripening: 51-70 DAT, which are the assessment phases of physiological maturation). Increasing salt concentrations in the nutrient solution reduced photosynthetic efficiency, stomatal conductance and transpiration, but increased the intercellular CO2 concentration in melon plants. A salt concentration in the low to intermediate range (2.5 dS m-1) resulted in the best water use efficiency by melon crops.

RESUMO A água salobra pode representar um grande potencial para a produção agrícola rentável, com base na adoção de práticas culturais adequadas e tolerância das culturas à salinidade, contrapondo aos problemas produtivos e de solo que normalmente ocasionam. Devido à escassez de informações sobre o cultivo hidropônico de melão, o presente estudo teve o objetivo de investigar as alterações fisiológicas provocadas pelo uso de água salobra no preparo da solução nutritiva para o cultivo de melão (Cucumis melo L., cv. AF 015) em ambiente protegido e em substrato de fibra de coco, em Mossoró-RN, região semiárido do Brasil. O delineamento experimental utilizado foi o inteiramente casualizado, com 12 tratamentos arranjados em esquema fatorial 4 x 3, sendo 4 níveis de concentração de sais (1,1 dS m-1 - controle, 2,5, 4,0 e 5,5 dS m-1) e 3 fases de desenvolvimento (vegetativo: 10-30 dias após o transplantio, DAT; floração: 31-50 DAT e frutificação: 51-70 DAT, que representam as fases de avaliação da maturação fisiológica) com três repetições. O aumento da concentração de sal da solução nutritiva reduziu a eficiência da fotossíntese, da condutância estomática e da transpiração e aumentou a concentração intracelular de CO2 em plantas de melão. A melhor eficiência do uso da água pela cultura do melão cultivada em fibra de coco foi alcançada na solução nutritiva com concentração de 2.5 dS m-1. | ABSTRACT Brackish waters represent great potential for profitable agricultural production; however, productive usage depends on the adoption of proper cultural practices as well as a culture tolerant of salinity, which can require some restrictions related to soil and crop production. Given the lack of information pertaining to hydroponic melon culture, the objective of this study was to investigate physiological changes promoted by the use of brackish water in the preparation of the nutrient solution for melon (Cucumis melo L., cv. AF 015) growth in coconut fiber substrate under greenhouse conditions in Mossoró-RN, a semiarid region of Brazil. The experimental design was completely randomized, with 12 treatments arranged in a 4 x 3 factorial scheme, with 4 salt concentration levels (1.1 - control, 2.5, 4.0 and 5.5 dS m-1) and 3 exposition times (vegetative growth: 10-30 days after transplanting, DAT; flowering: 31 to 50 DAT; and fruiting and ripening: 51-70 DAT, which are the assessment phases of physiological maturation). Increasing salt concentrations in the nutrient solution reduced photosynthetic efficiency, stomatal conductance and transpiration, but increased the intercellular CO2 concentration in melon plants. A salt concentration in the low to intermediate range (2.5 dS m-1) resulted in the best water use efficiency by melon crops.

The genetic variability within the crop species Ipomoea batatas is broad, hence, in order to support future breeding programs it is of the utmost importance that germplasm banks be created, conserved, and characterized. Therefore, the objective of this work was to rescue and evaluate the genetic divergence in sweet potato accessions collected in traditional communities of Vale do Ribeira Paulista. Sweet potato samples were collected from quilombos, indigenous villages, caiçaras communities, and small farms. The study was conducted between February 2013 and August 2014 in a randomized block design with three replications. Genetic material included 95 collected accessions and two commercial cultivars. Morphological characteristics of the accessions were evaluated and distances in the genetic distance matrix were estimated by means of multi-category variables, the data being subsequently clustered by the Tocher method. Analysis of the relative contribution of each characteristic and phenotypic correlation of descriptors was also performed. Results evidenced wide genetic diversity among the sweet potato accessions collected in Vale do Ribeira, which were not grouped according to the collection point. The descriptors that contributed more than 60% of genetic diversity included: leaf size, general leaf profile, immature leaf color, petiole pigmentation, predominant branch color, branch secondary color, stem length, cortical thickness, predominant periderm color, and periderm color intensity. Correlations between morphological descriptors was observed in 22.26% of the paired traits.

ABSTRACT The genetic variability within the crop species Ipomoea batatas is broad, hence, in order to support future breeding programs it is of the utmost importance that germplasm banks be created, conserved, and characterized. Therefore, the objective of this work was to rescue and evaluate the genetic divergence in sweet potato accessions collected in traditional communities of Vale do Ribeira Paulista. Sweet potato samples were collected from quilombos, indigenous villages, caiçaras communities, and small farms. The study was conducted between February 2013 and August 2014 in a randomized block design with three replications. Genetic material included 95 collected accessions and two commercial cultivars. Morphological characteristics of the accessions were evaluated and distances in the genetic distance matrix were estimated by means of multi-category variables, the data being subsequently clustered by the Tocher method. Analysis of the relative contribution of each characteristic and phenotypic correlation of descriptors was also performed. Results evidenced wide genetic diversity among the sweet potato accessions collected in Vale do Ribeira, which were not grouped according to the collection point. The descriptors that contributed more than 60% of genetic diversity included: leaf size, general leaf profile, immature leaf color, petiole pigmentation, predominant branch color, branch secondary color, stem length, cortical thickness, predominant periderm color, and periderm color intensity. Correlations between morphological descriptors was observed in 22.26% of the paired traits. | RESUMO A variabilidade genética existente dentro da espécie Ipomoea batatas é amplo, assim, visando dar suporte a futuros programa de melhoramento genético da espécies é de suma importância que bancos de germoplasma sejam formados, conservados e caracterizados. Desta forma, objetivou com este trabalho resgatar e avaliar a divergência genética em acessos coletados em comunidades tradicionais do Vale do Ribeira Paulista. A condução do estudo foi realizada em campo da Fazenda da UNESP-Registro entre fevereiro/2013 a agosto/2014. O experimento foi conduzido em blocos casualizados, com 95 acessos coletados e duas cultivares comerciais, com três repetições. Foram avaliadas características morfológicas da parte aérea e da raiz dos acessos segundo a metodologia de Huamán. A estimativa da matriz da distância genética foi realizada por meio das variáveis multicategoricas, sendo posteriormente os dados agrupados pelo método de Tocher. Também realizou-se análise da contribuição relativa de cada característica e correlação fenotípica dos descritores. De acordo com os resultados, pode-se concluir que há ampla diversidade genética entre os acessos de batata-doce coletados no Vale do Ribeira; os acessos não agruparam em função do ponto de coleta; os descritores que contribuíram com mais de 60% da divergência genética foram o tamanho da folha, perfil geral da folha, cor da folha imatura, pigmentação do pecíolo, coloração predominante das ramas, cor secundária das ramas, comprimento da haste, espessura do córtex, cor predominante da periderme e intensidade da cor da periderme; A correlação entre os descritores morfológicos foi verificada em 22,26% dos pares.

Safflower crops is usually intended for production of seeds to oil extraction. The objective of this work was to evaluate the chlorophyll index, biometric characteristics and water use efficiency of safflower plants grown under different nitrogen and potassium rates and controlled soil water tension. The experiment was conducted in a greenhouse, in pots containing 8 dm3 of a Oxisol. A complete randomized block experimental design in a 5x5 factorial arrangement was used, with four replications, consisting of five nitrogen rates (0, 60, 120, 180 and 240 mg dm-3) and five potassium rates (0, 50, 100, 150 and 200 mg dm-3). The safflower number of leaves, stem diameter, chlorophyll index, number of capitula, shoot, root and total dry weights, water consumption and water use efficiency were evaluated. Data were subjected to analysis of variance (F test) at 5% of probability and to polynomial regression. The nitrogen and potassium fertilization, as independent factors, affected the safflower plants. The chlorophyll index, biometric characteristics and water use efficiency of safflower showed better responses under nitrogen rates of 82 to 168 mg dm-3. The responses of chlorophyll index, biometric characteristics and water use efficiency of safflower increased 10 to 60% with potassium fertilization.

ABSTRACT Safflower crops is usually intended for production of seeds to oil extraction. The objective of this work was to evaluate the chlorophyll index, biometric characteristics and water use efficiency of safflower plants grown under different nitrogen and potassium rates and controlled soil water tension. The experiment was conducted in a greenhouse, in pots containing 8 dm3 of a Oxisol. A complete randomized block experimental design in a 5x5 factorial arrangement was used, with four replications, consisting of five nitrogen rates (0, 60, 120, 180 and 240 mg dm-3) and five potassium rates (0, 50, 100, 150 and 200 mg dm-3). The safflower number of leaves, stem diameter, chlorophyll index, number of capitula, shoot, root and total dry weights, water consumption and water use efficiency were evaluated. Data were subjected to analysis of variance (F test) at 5% of probability and to polynomial regression. The nitrogen and potassium fertilization, as independent factors, affected the safflower plants. The chlorophyll index, biometric characteristics and water use efficiency of safflower showed better responses under nitrogen rates of 82 to 168 mg dm-3. The responses of chlorophyll index, biometric characteristics and water use efficiency of safflower increased 10 to 60% with potassium fertilization. | RESUMO O cártamo destina-se à produção de grãos para a obtenção de óleo vegetal. Objetivou-se avaliar o índice de clorofila, as características biométricas e a eficiência no uso da água de plantas de cártamo sob doses de nitrogênio e potássio. O experimento foi conduzido em casa de vegetação, em vasos contendo 8 dm3 de um Latossolo Vermelho. O delineamento experimental foi blocos casualizados, com esquema fatorial 5x5 correspondente a cinco doses de nitrogênio (0, 60, 120, 180 e 240 mg dm-3) e cinco doses de potássio (0, 50, 100, 150 e 200 mg dm-3), com quatro repetições. Avaliou-se o número de folhas, diâmetro de caule, índice de clorofila, número de capítulos, massa seca da parte aérea, massa seca de raízes, massa seca total, consumo de água e eficiência no uso da água. Os dados foram submetidos a 5% de probabilidade de erro a análise de variância pelo teste F e regressão polinomial. O índice de clorofila, as características biométricas e a eficiência no uso da água das plantas de cártamo apresentaram melhores respostas nas doses de nitrogênio entre 82 a 168 mg dm-3. A adubação potássica nas plantas de cártamo proporcionou respostas crescentes com incrementos de 10 a 60% para o índice de clorofila, nas características biométricas e na eficiência de uso da água. As respostas a adubação com nitrogênio e potássio no cártamo ocorrem com efeito isolado.

Spatial variability of soil attributes affects crop development. Thus, information on its variability assists in soil and plant integrated management systems. The objective of this study was to assess the spatial variability of the soil apparent electrical conductivity (ECa), electrical conductivity of the saturation extract (ECse), water content in the soil (θ) and soil texture (clay, silt and sand) of a sugarcane crop area in the State of Pernambuco, Brazil. The study area had about 6.5 ha and its soil was classified as orthic Humiluvic Spodosol. Ninety soil samples were randomly collected and evaluated. The attributes assessed were soil apparent electrical conductivity (ECa) measured by electromagnetic induction with vertical dipole (ECa-V) in the soil layer 0.0.4 and horizontal dipole (ECa-H) in the soil layer 0.0-1.5 m; and ECse, θ and texture in the soil layers 0.0-0.2 m and 0.2-0.4 m. Spatial variability of the ECa was affected by the area relief, and had no direct correlation with the electrical conductivity of the saturation extract (ECse). The results showed overestimated mean frequency distribution, with means distant from the mode and median. The area relief affected the spatial variability maps of ECa-V, ECa-H, ECse and θ, however, the correlation matrix did not show a well-defined cause-and-effect relationship. Spatial variability of texture attributes (clay, site and sand) was high, presenting pure nugget effect.

ABSTRACT Spatial variability of soil attributes affects crop development. Thus, information on its variability assists in soil and plant integrated management systems. The objective of this study was to assess the spatial variability of the soil apparent electrical conductivity (ECa), electrical conductivity of the saturation extract (ECse), water content in the soil (θ) and soil texture (clay, silt and sand) of a sugarcane crop area in the State of Pernambuco, Brazil. The study area had about 6.5 ha and its soil was classified as orthic Humiluvic Spodosol. Ninety soil samples were randomly collected and evaluated. The attributes assessed were soil apparent electrical conductivity (ECa) measured by electromagnetic induction with vertical dipole (ECa-V) in the soil layer 0.0.4 and horizontal dipole (ECa-H) in the soil layer 0.0-1.5 m; and ECse, θ and texture in the soil layers 0.0-0.2 m and 0.2-0.4 m. Spatial variability of the ECa was affected by the area relief, and had no direct correlation with the electrical conductivity of the saturation extract (ECse). The results showed overestimated mean frequency distribution, with means distant from the mode and median. The area relief affected the spatial variability maps of ECa-V, ECa-H, ECse and θ, however, the correlation matrix did not show a well-defined cause-and-effect relationship. Spatial variability of texture attributes (clay, site and sand) was high, presenting pure nugget effect. | RESUMO A variabilidade espacial dos atributos do solo, interferem sobre o desenvolvimento dos cultivos. Assim, o conhecimento dessa variabilidade permite o manejo integrado de solo e planta. Objetivou-se determinar a variabilidade espacial da condutividade elétrica aparente (CEa), condutividade elétrica do extrato de saturação (CEes), conteúdo de água (θ) e textura (argila, silte e areia) do solo em uma área cultivada com cana-de-açúcar, no Estado de Pernambuco. A área de estudo possui cerca de 6,5 ha e o solo da área é um Espodossolo Humilúvico órtico. As amostras de solo foram avaliadas em 90 pontos de amostragem distribuídos aleatoriamente. Foram amostrados os seguintes atributos: condutividade elétrica aparente (CEa) medida por indução eletromagnética (dipolo vertical: CEa-V e dipolo horizontal: CEa-H) nas camadas de 0,0-0,4 m e 0,0-1,5 m de profundidade respectivamente. Os demais atributos foram medidos nas camadas de 0,0-0,2 m e 0,2-0,4 m de profundidade (CEes, θ, argila, silte e areia). A variabilidade espacial da condutividade elétrica aparente do solo medida por indução eletromagnética (CEa-V e CEa-H) foi influenciada pelo relevo, não apresentando relação direta com a condutividade elétrica do extrato de saturação do solo (CEes). Os atributos em estudo apresentaram distribuição de frequência com média superestimada, com valores de média se distanciando dos valores de moda e mediana. O relevo influenciou os mapas de variabilidade espacial da CEa-V, CEa-H, CEes e θ, apesar da matriz de correlação não demonstrar relação de causa e efeito bem definida. Os atributos texturais (argila, site e areia) apresentaram elevada variabilidade espacial, apresentando efeito pepita puro (EPP).

The objective of this work was to evaluate the interaction of microorganisms and phosphorus dosages in the development of gliricidia. The experimental design was completely randomized with six treatments (control, native microbial inoculant, and four arbuscular mycorrhizal fungi isolates: UFLA05 - Gigaspora albida, UFLA351 - Rhizoglomus clarum, UFLA372 - Claroideoglomus etunicatum, and UFLA401 - Acaulospora morrowiae), with four replicates. The parameters evaluated were: height plant, the number of branches, shoot and root dry mass matter, root length and volume, leaf phosphorus, mycorrhizal colonization, the number of spores of the arbuscular mycorrhizal fungi, the number of nodules of nitrogen fixing bacteria, and the presence of endophytic dark septate fungi, after 95 days of inoculation. The high mycorrhizal colonization of gliricidia does not guarantee an increase in biomass, which depends on the interaction of the arbuscular mycorrhizal fungi, the endophytic dark septate fungi, the nitrogen fixing bacteria, and the endophytic bacteria. Gliricidia was responsive to the inoculation of the native microbiota, UFLA372 and UFLA401. Mycorrhizal colonization by UFLA401 was influenced by the presence of nitrogen fixing bacteria. Gliricidia was not responsive to the inoculation of UFLA05 and UFLA351. The presence of the endophytic dark septate fungi did not inhibit mycorrhization and the formation of nodules of nitrogen fixing bacteria in gliricidia.

ABSTRACT The objective of this work was to evaluate the interaction of microorganisms and phosphorus dosages in the development of gliricidia. The experimental design was completely randomized with six treatments (control, native microbial inoculant, and four arbuscular mycorrhizal fungi isolates: UFLA05 - Gigaspora albida, UFLA351 - Rhizoglomus clarum, UFLA372 - Claroideoglomus etunicatum, and UFLA401 - Acaulospora morrowiae), with four replicates. The parameters evaluated were: height plant, the number of branches, shoot and root dry mass matter, root length and volume, leaf phosphorus, mycorrhizal colonization, the number of spores of the arbuscular mycorrhizal fungi, the number of nodules of nitrogen fixing bacteria, and the presence of endophytic dark septate fungi, after 95 days of inoculation. The high mycorrhizal colonization of gliricidia does not guarantee an increase in biomass, which depends on the interaction of the arbuscular mycorrhizal fungi, the endophytic dark septate fungi, the nitrogen fixing bacteria, and the endophytic bacteria. Gliricidia was responsive to the inoculation of the native microbiota, UFLA372 and UFLA401. Mycorrhizal colonization by UFLA401 was influenced by the presence of nitrogen fixing bacteria. Gliricidia was not responsive to the inoculation of UFLA05 and UFLA351. The presence of the endophytic dark septate fungi did not inhibit mycorrhization and the formation of nodules of nitrogen fixing bacteria in gliricidia. | RESUMO O objetivo deste trabalho foi avaliar a interação de micro-organismos no desenvolvimento da gliricídia. O delineamento experimental utilizado foi inteiramente aleatorizado composto por seis tratamentos (controle; inoculante microbiano nativo e quatro isolados fúngicos micorrízicos arbusculares: UFLA05 - Gigaspora albida, UFLA351 - Rhizoglomus clarum, UFLA372 - Claroideoglomus etunicatum e UFLA401 - Acaulospora morrowiae), com quatro repetições. Os parâmetros avaliados foram: altura da planta, número de ramos, massa da matéria seca da parte aérea e da raiz, comprimento e volume de raiz, teor de fósforo foliar, colonização micorrízica, número de esporos de fungos micorrízicos arbusculares, número de nódulos de bactérias fixadoras de nitrogênio, presença de fungos endofíticos "dark septate", após 95 dias da inoculação. A elevada colonização micorrízica da gliricídia não garante incremento da biomassa, a depender da interação de fungos micorrízicos arbusculares, de fungos endofíticos “dark septate”, de bactérias fixadoras do nitrogênio e de bactérias endofíticas. A gliricídia foi responsiva à inoculação da microbiota nativa, UFLA372 e UFLA401. A colonização micorrízica por UFLA401 foi influenciada pela presença de bactérias fixadoras do nitrogênio. A gliricídia não foi responsiva à inoculação de UFLA05 e UFLA351. A ocorrência de fungos endofíticos “dark septate” não inibiu a micorrização e a formação de nódulos de bactérias fixadoras do nitrogênio, em gliricídia.

Cowpea (Vigna unguiculata L. Walp.) is one of the most important legumes produced in tropical and subtropical regions throughout the world, especially in the developing countries in Africa, Latin America, and Asia. It is the main source of protein, calories, dietary fiber, minerals, and vitamins for a large segment of the world population. Cowpea is also a potential functional food with a range of bioactive compounds, including phenolic compounds. This legume is grown mainly in the North and Northeast regions of Brazil, but is also consumed in other regions, and is thus important for the farmers who depend on this crop for income. This study identified and quantified phenolic compounds in the cowpea cultivar BRS Xiquexique. Such quantification reveals the functional characteristics of cowpeas, mainly as a source of antioxidants, which will be essential to add value to this food and to expand its forms of consumption. The extracts were analyzed using an HPLC model LC-20AT, equipped with a manual injector. For the HPLC analysis, standard solutions were prepared with pure phenolic acids such as gallic acid, quercetin, caffeic acid, chlorogenic acid, ferulic acid, p-coumaric acid, catechin, and epicatechin. The major phenolic compounds identified were catechin (2.07± 0.329 mg 100 g-1), epicatechin (0.48 ± 0.130 mg 100 g-1), gallic acid (67.19 ± 6.200 mg 100 g-1), ferulic acid (32.07 ± 0.753 mg 100 g-1), and chlorogenic acid (3.08 ± 0.489 mg 100 g-1). We observed that the BRS Xiquexique cultivar contains functional phenolic compounds, especially gallic acid and ferulic acid, demonstrating the antioxidant potential of cowpea.

RESUMO O feijão-caupi (Vigna unguiculata [L.] Walp.) é uma das mais importantes leguminosas produzidas em regiões tropicais e subtropicais do mundo, principalmente nos países em desenvolvimento da África, América Latina e Ásia. Para um grande segmento da população mundial é a principal fonte de proteínas, calorias, fibras alimentares, minerais e vitaminas. Possui ainda compostos bioativos, destacando-se os compostos fenólicos. É produzido no Norte e Nordeste, consumido também em outras regiões do país e apresenta grande importância para os agricultores que sobrevivem desta cultura. O objetivo do presente estudo foi identificar e quantificar os compostos fenólicos no feijão-caupi, cultivar BRS Xiquexique. Tal determinação permitirá conhecer características funcionais do feijão-caupi, como fonte de antioxidantes, o que será de primordial importância para agregar valor funcional e nutricional a este alimento e ampliar as formas de consumo. Os extratos foram analisados por CLAE em equipamento modelo LC-20AT, com injetor manual. Para as análises foram preparadas soluções padrões com os ácidos fenólicos puros, tais como: ácido gálico, quercetina, ácido cafeico, ácido clorogênico, ácido ferúlico, ácido p-cumárico, catequina e epicatequina. Foram identificados catequina (2,07 ± 0,329 mg 100 g-1), epicatequina (0,48 ± 0,130 mg 100 g-1), ácido gálico (67,19 ± 6,200 mg 100 g-1), ácido ferúlico (32,07 ± 0,753 mg 100 g-1) e ácido clorogênico (3,08 ± 0,489 mg 100 g-1) em concentrações importantes. Concluiu-se que a cultivar BRS Xiquexique contêm compostos funcionais, destacando-se o ácido gálico e o ácido ferúlico com maiores teores, comprovando o potencial antioxidante do feijão-caupi. | ABSTRACT Cowpea (Vigna unguiculata [L.] Walp.) is one of the most important legumes produced in tropical and subtropical regions throughout the world, especially in the developing countries in Africa, Latin America, and Asia. It is the main source of protein, calories, dietary fiber, minerals, and vitamins for a large segment of the world population. Cowpea is also a potential functional food with a range of bioactive compounds, including phenolic compounds. This legume is grown mainly in the North and Northeast regions of Brazil, but is also consumed in other regions, and is thus important for the farmers who depend on this crop for income. This study identified and quantified phenolic compounds in the cowpea cultivar BRS Xiquexique. Such quantification reveals the functional characteristics of cowpeas, mainly as a source of antioxidants, which will be essential to add value to this food and to expand its forms of consumption. The extracts were analyzed using an HPLC model LC-20AT, equipped with a manual injector. For the HPLC analysis, standard solutions were prepared with pure phenolic acids such as gallic acid, quercetin, caffeic acid, chlorogenic acid, ferulic acid, p-coumaric acid, catechin, and epicatechin. The major phenolic compounds identified were catechin (2.07± 0.329 mg 100 g-1), epicatechin (0.48 ± 0.130 mg 100 g-1), gallic acid (67.19 ± 6.200 mg 100 g-1), ferulic acid (32.07 ± 0.753 mg 100 g-1), and chlorogenic acid (3.08 ± 0.489 mg 100 g-1). We observed that the BRS Xiquexique cultivar contains functional phenolic compounds, especially gallic acid and ferulic acid, demonstrating the antioxidant potential of cowpea.

Bacillus spp. have been used against diseases caused by bacteria that affect cultured shrimp, providing beneficial effects on the host shrimps by altering their microbial community, and improving zootechnical indexes. The objective of this work was to evaluate the effects of a diet supplemented with Bacillus cereus-a bacterium with probiotic potential-on post-larvae Litopenaeus vannamei shrimps grown in laboratory. The experiment lasted for fifteen days and consisted of six treatments-control (T1), probiotic (T2), Vibrio parahaemolyticus (VP) (T3), probiotic and VP (T4), V. alginolyticus (VA) (T5), and probiotic and VA (T6). The survival rate, weight gain, colonization capacity of the probiotic bacteria, pathogen count, and histopathological lesions were evaluated. There was no significant difference (p=0.05) in survival between treatments. The groups with pathogens and without probiotics presented lower weight gain. The result of the Bacillus cereus count in the treatments T2, T4 and T6 were significantly different (p<0.05), the probiotic bacteria were more aggressive in competing for space and nutrients when compared to V. parahaemolyticus than when compared to V. alginolyticus. Animals fed with the probiotic presented lower counts of these pathogens than those fed without the probiotic (p<0.05). No histopathological lesions were found in the organs and tissues of the shrimps. Bacillus cereus showed a high colonizing capacity in post-larvae shrimps, causing a significant reduction of pathogens, probably by secreting antimicrobial substances and the competitive exclusion, which justifies their use as probiotic bacteria.

RESUMO Bacillus spp. têm sido utilizados contra bacterioses que acometem camarão marinho cultivado, proporcionando efeito benéfico sobre o hospedeiro, alterando a comunidade microbiana intestinal e melhorando índices zootécnicos. O objetivo deste trabalho foi avaliar os efeitos de uma dieta suplementada com Bacillus cereus, bactéria com potencial probiótico em pós-larvas de Litopenaeus vannamei cultivados em laboratório. O experimento teve duração de quinze dias e consistiu de seis tratamentos: T1 controle; T2 somente com probiótico; T3 com Vibrio parahaemolyticus (VP); T4 com probiótico e com VP; T5 com V. alginolyticus (VA); e T6 com probiótico e com VA. Foram avaliados a sobrevivência, o ganho de peso, a capacidade de colonização das bactérias probióticas, contagem de patógenos e lesões histopatológicas. Não foi verificada diferença significativa (P=0,05) entre os tratamentos na sobrevivência. Os grupos desafiados com patógenos e sem uso de probióticos foram os que apresentaram menor ganho de peso. Na contagem de Bacillus cereus, houve diferença significativa (P<0,05) entre os tratamentos T2, T4 e T6 , observando-se que o probiótico competiu melhor por espaço e nutrientes quando confrontado com V. parahaemolyticus do que com V. alginolyticus. Os animais alimentados com ração suplementada de probiótico, apresentaram contagem inferior daqueles alimentados sem o uso (P<0,05). Não foram observadas lesões histopatológicas nos órgãos e tecidos dos animais. O Bacillus cereus demonstrou uma alta capacidade de colonizar pós-larvas de camarão, causando uma diminuição significativa de patógenos, provavelmente pela secreção de substâncias antimicrobianas e/ou por exclusão competitiva, justificando seu uso como uma bactéria probiótica. | ABSTRACT Bacillus spp. have been used against diseases caused by bacteria that affect cultured shrimp, providing beneficial effects on the host shrimps by altering their microbial community, and improving zootechnical indexes. The objective of this work was to evaluate the effects of a diet supplemented with Bacillus cereus-a bacterium with probiotic potential-on post-larvae Litopenaeus vannamei shrimps grown in laboratory. The experiment lasted for fifteen days and consisted of six treatments-control (T1), probiotic (T2), Vibrio parahaemolyticus (VP) (T3), probiotic and VP (T4), V. alginolyticus (VA) (T5), and probiotic and VA (T6). The survival rate, weight gain, colonization capacity of the probiotic bacteria, pathogen count, and histopathological lesions were evaluated. There was no significant difference (p=0.05) in survival between treatments. The groups with pathogens and without probiotics presented lower weight gain. The result of the Bacillus cereus count in the treatments T2, T4 and T6 were significantly different (p<0.05), the probiotic bacteria were more aggressive in competing for space and nutrients when compared to V. parahaemolyticus than when compared to V. alginolyticus. Animals fed with the probiotic presented lower counts of these pathogens than those fed without the probiotic (p<0.05). No histopathological lesions were found in the organs and tissues of the shrimps. Bacillus cereus showed a high colonizing capacity in post-larvae shrimps, causing a significant reduction of pathogens, probably by secreting antimicrobial substances and the competitive exclusion, which justifies their use as probiotic bacteria.