Pathogenic microorganisms are a serious threat to global health, that involves high costs of the treatment and their fight. We proposed to evaluate PAW antimicrobial activity against the microorganisms food-borne pathogens. PAW It was observed that due to the reactive species in water shows a post-discharge an antimicrobial effect on food-borne pathogens showed until a few days after discontinuation of plasma discharge in water. The logarithmic reduction was higher than 5log10 after an exposure time of 5-min, exhibiting a powerful bactericidal effect. Significant reduction in bacterial population were achieved in all type strains, demonstrating the effectiveness of this new approach to treat the food borne pathogens. Thus, non-thermal plasma activated water can be a promising alternative to traditional disinfection applied in food industry.