Currently, the spread of micro- and nanoplastics in the food chain and the environment has become a relevant and social issue. They enter the human body mainly through food, water and drinks packaged in various plastic materials, bottles or containers. It has been established that most urban water sources are contaminated with microplastics due to wastewater. The main objective of the article was to study the awareness of microplastic sources, exposure levels and potential health risks associated with microplastic contamination of food and water by surveying a heterogeneous audience of Internet users. The preventive focus of the survey included: 1) holding an action at the medical faculty of KRSU “Day without plastic”; 2) development, publication and distribution of an information leaflet in the Internet community on the key sources of microplastics in the daily human diet. The test group consisted of 502 Internet users. The author's questionnaire included 26 questions: personal data (5 questions), the size of microplastics, sources of information, an assessment of the risk factors for microplastics entering the body, its content in food and water and the impact on human health (a list of 16 diseases associated with microplastics is provided). Statistical processing of the obtained results was carried out using SPSS version 28.0.1 software. Questionnaires of 502 respondents aged 14–50 years were obtained through an online survey from October 2023 to January 2024 using Google forms. The demographic structure reflected the predominance of female representatives - 52.4%, men accounted for 47.6%. In terms of education level, students dominated among the subjects, incomplete higher education (67.3%), then secondary (14.8%), higher (9.9%) and primary education (8.8%). Most of the respondents lived in the city (80.3%), compared to rural areas (19.7%). Since the survey involved young people, 49.8% of respondents correctly answered the question that microplastics are small particles measuring 5 microns. 50.2% of respondents answered incorrectly, indicating sizes of 10 and 15 microns. Respondents noted that microplastics are most often found in bottled water (29.5%), tea bags (17.3%), natural water (10.3%), fish (8.1%), salt (6.3%), seafood (5.5%) and honey (4.5%). All of the above were noted by 29.5% of respondents. Most respondents to the question: which vegetables are most often contaminated with microplastics, answered that none (38.5%), followed by root vegetables (30.4%), onions (13.1%), cabbage (9.7%) and legumes (8.3%). Among the presented sample - the correct answer is - root crops are most susceptible to contamination. The results of the survey show that in everyday life 45.8% of respondents most often used plastic bags (55.7%), food packaging (18.7%), then cosmetics and hygiene products (17.4%), toys (5.9%) and cutlery (2.3%). Living in a “plastic world”, Internet users somehow do not notice it, to the question of how often the respondents use plastic products, 64.7% of them answered that every day, 26.7% - sometimes, 8.6% — rarely. As the results of the survey showed, most often Internet users received information about the harm of microplastics from social networks 26.5%, Google search engine — 20.7%, scientific articles — 18.9%, news — 17.1%, while communicating with friends, family — 5.5%. At the same time, 11.3% of respondents were not interested in the problem. When comparing the assessment of awareness of the direct and indirect impact of plastic on human health during plastic production and consumer use of plastics, they were aware (46.0% and 37.1%, respectively), slightly aware (31.9% and 37.8%) and did not know (22.1% and 25.1%). The risk that plastic pollution poses to wildlife, as well as to human health, well-being and prosperity, was assessed by respondents as average. 34.6% of respondents were in favor of actively reducing the use of plastic products. 33.7% of survey participants are still at the level of awareness of the problem and attempts to reduce plastic consumption. 31.7% find it difficult to answer, since they do not see an alternative. When asked about awareness of the impact of microplastics (the silent killer) on the development of 16 specific diseases, respondents were most aware of: digestive diseases, allergies, chronic inflammation, respiratory problems, cancer and reproductive problems.

Most fossil fuel-derived polymers used for food packaging are non-biodegradable and induce pollution by microplastic, calling for safer material. Here we review microbial production and applications of pullulan, a unique biopolymer produced by fermentation of agro-residues, using a strain named Aureobasidium pullulan. Chemically modified pullulan is widely used in food, pharmaceuticals, biomedical, and cosmetics. Compared to conventional polymers, pullulan increases the tensile strength 6–37-folds and increases the bioadhesion time 72–120-folds. Pullulan has been recently produced from agro-based waste with yields as high as 58-69 g/L.

In recent years, the presence of microplastics (MPs) and nanoplastics (NPs) has been assessed in several environmental matrices, including the marine environment and agricultural soil, suggesting those pollutants are likely to enter the food web. However, there is still a severe lack of information about the occurrence of plastic particles in our food, partially due to the multidimensionality of the data necessary to fully describe MP contamination and the consequent difficulty in validating analytical methods. In this review, consisting of two parts, preliminary results about the presence of MPs in food, water, and beverages are summarized (Part I) and several approaches for the characterization of micro- and nano-sized plastic particles are reported and discussed (Part II). The information gathered in this manuscript highlights the need for a more comprehensive knowledge of MP/NP occurrence along the food chain in order to assess the food safety risk related to those contaminants and implement strategies for their monitoring in products intended for human consumption. Therefore, an outlook of the field towards a coherent, consistent, and policy-relevant data collection and standardization is included in this review.

Microplastics are extremely complex, and as the food chain comes full circle, it is dreaded that these could have a deleterious influence on humans. Although the risk of plastics to humans is not yet established, their occurrence in food and water destined for human consumption has been reported. The prevalence of micro-sized plastics in the ecosystem and living organisms, their trophic transfer along the food web, and the discernment of food species as competent indicators have become research priorities. The scale of the issue is massive, but what are the main culprits and causes, and could there be a solution in sight for this global problem? Despite the massive amount of research in the field, a collation of available data and pertinent hazard evaluation remains difficult. In order to identify the knowledge gaps and exposure pathways, several traits related to food chain assessment are presented with the goal of properly evaluating and managing this emerging risk. We apprehend three possible noxious consequences of small plastic particles, firstly, due to the plastic particles themselves; secondly, due to the extrication of tenacious organic pollutants adsorbed onto the plastics; and thirdly, due to the leaching of components such as monomers and additives from the plastics. The exigency for the standardization of protocols to bring about consistency in data collection and analysis, involving solutions, stakeholder costs, and benefits, are discussed. Harmonized methods will enable meticulous assessment of the impacts and threats that microplastics pose to the biota and increase the comparability between studies. We emphasize the contribution of the “honest broker” in science, providing an overarching analysis to devise the most viable solutions to microplastic pollution for private and public leadership to utilize.

Despite the established presence of microplastics in consumable products and inhalable air and the associated health hazards, the actual extent of human exposure to microplastics is currently unknown. We estimated exposure to microplastics through 24-h composite sampling of drinking water, cooked food, and respirable air. Daily average exposures of 382 ± 205, 594 ± 269, and 1036 ± 493 particles per person were observed through drinking water, air, and food, respectively. Our estimates suggest an average daily exposure of 2012 ± 598 microplastic particles per person via these dominant routes. Considering the variety of common consumer plastics, the plastic intake was calculated to be 122.25 ± 177.38 to 202.80 ± 294.25 mg per person per day. Food ingestion was observed to be the major pathway, with fragments as the dominant particle type, followed by fibers and spherical beads. The major polymers identified in the food samples were polyethylene terephthalate, polystyrene, polynorbornene, nylon, polychloroprene, and copolymer polyacrylamide. These results provide a realistic estimate of urban exposure to microplastics and may be helpful in their risk characterization.

Microplastics pervade the hydrosphere and inevitably come into contact with aquatic organisms. The study reports quantitative data on absorption and excretion of polystyrene microspheres 2 and 10 µm in diameter by zooplankton and fish larvae on the example of Artemia salina L. and Acipenser rithenus L. At the initial concentration of 500 µg/L, A. salina accumulated 2 and 10 µm particles in amounts up to 0.103 and 0.151 ng/individual, respectively, at a similar rate. The mass content of large-sized particles in A. salina was significantly higher (p < 0.01) compared to small-sized particles throughout the experiment. Artemia salina and A. rithenus larvae did not accumulate microplastics in the gastrointestinal tract over a period of 96 and 72 h, respectively. Consumption of microplastics by A. ruthenus larvae with A. salina through the food chain was slower and less pronounced in mass than their direct absorption from water. The rates of absorption of 2 and 10 μm particles by fish attained 0.9 and 8.22 ng/individual/h from water, and 0.06 and 0.23 ng/individual/h with food, respectively. In the models of water pollution and food chain transfer, A. ruthenus larvae consumed more 10 µm particles in mass compared to 2 µm particles (p < 0.05) and at a higher rate. For 2 µm particles, the excretion time for 50% of particles from the gastrointestinal tract of fish (T50) was 32–33 h, whereas for 10 µm particles, the excretion of particles consumed with food was slower (T50=45 h) compared to that of particles absorbed directly from water (T50=25 h). The data obtained can be used to simulate transport and circulation of microplastics of different sizes in the environment.

Microplastics pervade the hydrosphere and inevitably come into contact with aquatic organisms. The study reports quantitative data on absorption and excretion of polystyrene microspheres 2 and 10 µm in diameter by zooplankton and fish larvae on the example of Artemia salina L. and Acipenser rithenus L. At the initial concentration of 500 µg/L, A. salina accumulated 2 and 10 µm particles in amounts up to 0.103 and 0.151 ng/individual, respectively, at a similar rate. The mass content of large-sized particles in A. salina was significantly higher (p &lt; 0.01) compared to small-sized particles throughout the experiment. Artemia salina and A. rithenus larvae did not accumulate microplastics in the gastrointestinal tract over a period of 96 and 72 h, respectively. Consumption of microplastics by A. ruthenus larvae with A. salina through the food chain was slower and less pronounced in mass than their direct absorption from water. The rates of absorption of 2 and 10 μm particles by fish attained 0.9 and 8.22 ng/individual/h from water, and 0.06 and 0.23 ng/individual/h with food, respectively. In the models of water pollution and food chain transfer, A. ruthenus larvae consumed more 10 µm particles in mass compared to 2 µm particles (p &lt; 0.05) and at a higher rate. For 2 µm particles, the excretion time for 50% of particles from the gastrointestinal tract of fish (T50) was 32–33 h, whereas for 10 µm particles, the excretion of particles consumed with food was slower (T50=45 h) compared to that of particles&nbsp;absorbed directly from water (T50=25 h). The data obtained can be used to simulate transport and circulation of microplastics of different sizes in the environment.

In this study, surface seawater, sediment and zooplankton samples were collected from three different sampling stations in Marseille Bay (NW Mediterranean Sea) and were analyzed for both microplastics and organic plastic additives including seven phthalates (PAEs) and nine organophosphate esters (OPEs). PAE concentrations ranged from 100 to 527 ng L⁻¹ (mean 191 ± 123 ng L⁻¹) in seawater, 12–610 ng g⁻¹ dw (mean 194 ± 193 ng g⁻¹ dw) in sediment and 0.9–47 μg g⁻¹ dw (mean 7.2 ± 10 μg g⁻¹ dw) in zooplankton, whereas OPE concentrations varied between 9 and 1013 ng L⁻¹ (mean 243 ± 327 ng L⁻¹) in seawater, 13–49 ng g⁻¹ dw (mean 25 ± 11 ng g⁻¹ dw) in sediment and 0.4–4.6 μg g⁻¹ dw (mean 1.6 ± 1.0 μg g⁻¹ dw) in zooplankton. Microplastic counts in seawater ranged from 0 to 0.3 items m⁻³ (mean 0.05 ± 0.05 items m⁻³). We observed high fluctuations in contaminant concentrations in zooplankton between different sampling events. However, the smallest zooplankton size class generally exhibited the highest PAE and OPE concentrations. Field-derived bioconcentration factors (BCFs) showed that certain compounds are prone to bioaccumulate in zooplankton, including some of the most widely used chlorinated OPEs, but with different intensity depending on the zooplankton size-class. The concentration of plastic additives in surface waters and the abundance of microplastic particles were not correlated, implying that they are not necessarily good indicators for each other in this compartment. This is the first comprehensive study on the occurrence and temporal variability of PAEs and OPEs in the coastal Mediterranean based on the parallel collection of water, sediment and differently sized zooplankton samples.

In this study, surface seawater, sediment and zooplankton samples were collected from three different sampling stations in Marseille Bay (NW Mediterranean Sea) and were analyzed for both microplastics and organic plastic additives including seven phthalates (PAEs) and nine organophosphate esters (OPEs). PAE concentrations ranged from 100 to 527 ng L-1 (mean 191±123 ng L-1) in seawater, 12 to 610 ng g-1 dw (mean 194±193 ng g-1 dw) in sediment and 0.9 to 47 μg g-1 dw (mean 7.2±10 μg g-1 dw) in zooplankton, whereas OPE concentrations varied between 9-1013 ng L-1 (mean 243±327 ng L-1) in seawater, 13-49 ng g-1 dw (mean 25±11 ng g-1 dw) in sediment and 0.4-4.6 μg g-1 dw (mean 1.6±1.0 μg g-1 dw) in zooplankton. Microplastic counts in seawater ranged from 0 to 0.3 items m-3 (mean 0.05±0.05 items m-3). We observed high fluctuations in contaminant concentrations in zooplankton between different sampling events. However, the smallest zooplankton size class generally exhibited the highest PAE and OPE concentrations. Field-derived bioconcentration factors (BCFs) showed that certain compounds are prone to bioaccumulate in zooplankton, including some of the most widely used chlorinated OPEs, but with different intensity depending on the zooplankton size-class. The concentration of plastic additives in surface waters and the abundance of microplastic particles were not correlated, implying that they are not necessarily good indicators for each other in this compartment. This is the first comprehensive study on the occurrence and temporal variability of PAEs and OPEs in the coastal Mediterranean based on the parallel collection of water, sediment and differently sized zooplankton samples.