The use of fluidized-bed bioreactors in waste and drinking water treatment has several advantages, the most significant of which is the specific removal rate, which is an order of magnitude higher than that of equivalent activated sludge processes. In this paper, the usual concept of nitrification-denitrification in separated units is replaced by a new concept in which the two processes are performed together in a single high-performance fluidized-bed. Based on the former nitrifying reactor, new equipment was designed. This reactor contained a fluidized bed with separated aerobic and anoxic sections for nitrification and denitri fication respectively. This was accomplished by the use of different-diameter sand as carrier material and appropriate reactor shape, recirculation, feed and aeration conditions. The reactor (20 L fluidized-bed volume) was operated for 3 months. It was fed with synthetic waste water (50 L/h) containing 25-40 mg NH₄ ⁺-N/L. Propionic acid and ethanol in a 1:4 ratio were used as the carbon source (2.3 g C/L) for deni trification, fed in at different points of the reactor. Ammonium removal reached 50%, while denitrification was 75%. The total nitrogen removal rate was 0.8-1.2 kg N/m ³.d. A new simple hydrostatic pressure method was used to monitor biofilm thickness in the fluidized bed. During the experiments the oxidation-reduc tion potential (ORP) was tested as a tool to monitor reactor performance; its use for the control of the process was found to be limited.

Microbes live throughout the soil profile. Microbial communities in subsurface horizons are impacted by a saltwater–freshwater transition zone formed by seawater intrusion (SWI) in coastal regions. The main purpose of this study is to explore the changes in microbial communities within the soil profile because of SWI. The study characterizes the depth-dependent distributions of bacterial and archaeal communities through high-throughput sequencing of 16S rRNA gene amplicons by collecting surface soil and deep core samples at nine soil depths in Longkou City, China. The results showed that although microbial communities were considerably impacted by SWI in both horizontal and vertical domains, the extent of these effects was variable. The soil depth strongly influenced the microbial communities, and the microbial diversity and community structure were significantly different (p < 0.05) at various depths. Compared with SWI, soil depth was a greater influencing factor for microbial diversity and community structure. Furthermore, soil microbial community structure was closely related to the environmental conditions, among which the most significant environmental factors were soil depth, pH, organic carbon, and total nitrogen.