Se estudió la relación de la temperatura del agua con la distribución de las especies presentes en el área. Las observaciones fueron realizadas en tres secciones del río Lluta, donde se registraron las temperaturas a las 7.00 AM y 7.00 PM y se colectaron larvas y pupas en sustratos naturales como piedras y vegetación sumergida, procediendo posteriormente a su identificación taxonómica. Los datos obtenidos se analizaron estadísticamente utilizando el coeficiente de correlación de Pearson entre la temperatura media del agua y la cantidad de individuos colectados de cada especie. Las correlaciones obtenidas fueron en S. escomeli de r = 0,774, S. philippii de r = -0,442 y S. quechuanum r = -0,142. Durante el período de observación la especie S. escomeli presentó una correlación positiva intensa respecto a la temperatura del agua. | The relation of the temperature of the water with the distribution of the present species in the area was studied.. The observations were made in three sections of the Lluta River, where they registered the temperature to 7:00 A.M. and 7:00 P.M. and collected larvae and pupae in natural substrates like stones and submerged vegetation, coming later to its taxonomic identification. The collected data were analyzed statistically using the coefficient of correlation of Pearson between the average temperature of the water and the amount of individuals collected of each species. The obtained correlations were in S. escomeli of r = 0.774, S. philippii of r = -0.442 and S. quechuanum r = -0.142. During the period of observation S. lescomeli presented intense positive correlation with respect to the temperature of the water.

The goals of this study were to develop methodological changes for applying the bio-pesticide Teknar ® to control the black fly in streams with irregular flow and identify black fly species in three streams (Cafezal, Cambé, Tamapuã) in Southern Brazil. The only regular-flow stream Cafezal was the control stream; the other two streams had irregular flow and were selected to validate the new methodology. The traditional method for bio-insecticide application in the streams under study presented larval mortalities of 99.13% (Cafezal), 59.76% (Cambé), and 13.87% (Tamapuã) . As a result of the new methodology, bio-insecticide concentration was determined according to the specific irregularities of the Tamapuã and Cambé streams and mortality percentages increased to 99.43% and 99.32%, respectively. Among the 229,251 immature Simuliidae collected during the experiment, Grenieriella pruinosum and Chirostilbia pertinax were the most abundant.<p><p>Objetivando desenvolver adaptações metodológicas para aplicação de bioinseticida Teknar® no controle de simulídeos em ribeirões com fluxo de água irregular e identificar as espécies presentes, realizou-se experimentos nos ribeirões Cafezal, Cambé e Tamapuã, localizados no sul do Brasil. O primeiro, com fluxo de água regular de água, foi considerado como controle e os outros dois, com fluxos irregulares, foram as testemunhas para validação da nova metodologia proposta. Na metodologia tradicional, normalmente empregada para a aplicação do produto nos ribeirões considerados, foram alcançados as mortalidades larval de 99,13%, 59,76% e 13,87%, respectivamente. Para a nova metodologia, considerando no cálculo da concentração do produto aplicado as irregularidades específicas as ribeirões Cambe e Tamapuã, essas percentagens aumentaram para 99,43% e 99,32%. Durante os experimentos foram coletadas 229.251 imaturos, sendo Grenieriella pruinosum e Chirostilbia pertinax, as mais abundantes nesta região.