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Rhizobium sp. – a Potential Tool for Improving Protein Content in Peas and Faba Beans Full text
2017
Šenberga, Alise | Dubova, Laila | Alsiņa, Ina | Strauta, Liene
Rhizobium sp. – a Potential Tool for Improving Protein Content in Peas and Faba Beans Full text
2017
Šenberga, Alise | Dubova, Laila | Alsiņa, Ina | Strauta, Liene
Legume seed inoculation prior to sowing is a well-known practice in agriculture. Nitrogen fixation, due to the symbiotic relationship between legumes and rhizobia, improves the productivity of legumes. Rhizobia strain specificity can be observed very often, leading to differences in the total protein content. In this study two faba bean cultivars (‘Karmazyn’ and ‘Bartek’) and five pea cultivars (‘Retrija’, ‘Zaiga’, ‘Lāsma’, ‘Vitra’ and ‘Bartek’) were tested using various rhizobia strains. In addition, strain effectivity was observed in four different soil types. Overall, the protein content increase was observed after seed inoculation with Rhizobium sp. Rhizobia strain and plant cultivar interaction specification was observed. Plant cultivar appeared to have a decisive role in the formation of protein content when inoculated with Rhizobium sp. From these pilot experiments, it can be concluded that, when choosing Rhizobium sp. strains for legume inoculation, soil type also should be considered. Rhizobia has the potential to be used as a commercial preparation intended for increasing legume protein content, alongside with increased legume yield; however, different rhizobia strains should be mixed together to achieve the optimal result.
Show more [+] Less [-]Rhizobium sp. – a potential tool for improving protein content in peas and faba beans Full text
2017
Senberga, A., Latvia Univ. of Agriculture, Jelgava (Latvia) | Dubova, L., Latvia Univ. of Agriculture, Jelgava (Latvia) | Alsina, I., Latvia Univ. of Agriculture, Jelgava (Latvia) | Strauta, L., Latvia Univ. of Agriculture, Jelgava (Latvia)
Legume seed inoculation prior to sowing is a well-known practice in agriculture. Nitrogen fixation, due to the symbiotic relationship between legumes and rhizobia, improves the productivity of legumes. Rhizobia strain specificity can be observed very often, leading to differences in the total protein content. In this study two faba bean cultivars (‘Karmazyn’ and ‘Bartek’) and five pea cultivars (‘Retrija’, ‘Zaiga’, ‘Lāsma’, ‘Vitra’ and ‘Bartek’) were tested using various rhizobia strains. In addition, strain effectivity was observed in four different soil types. Overall, the protein content increase was observed after seed inoculation with Rhizobium sp. Rhizobia strain and plant cultivar interaction specification was observed. Plant cultivar appeared to have a decisive role in the formation of protein content when inoculated with Rhizobium sp. From these pilot experiments, it can be concluded that, when choosing Rhizobium sp. strains for legume inoculation, soil type also should be considered. Rhizobia has the potential to be used as a commercial preparation intended for increasing legume protein content, alongside with increased legume yield; however, different rhizobia strains should be mixed together to achieve the optimal result.
Show more [+] Less [-]Evaluation of patatin content in proteins of potato genotypes grown in Latvia Full text
2021
Berga, Dace | Sterna, Vita | Sokolova, Elina | Taskova, Inese | Seile, Sanita | Dimante, Ilze | Skrabule, Ilze
Potato proteins contains essential amino acids in considerably high concentration, therefore potatoes are considered to be one of the most valuable plant origin food for human consumption. Patatin forms one of the largest group of potato proteins with high potential to be used in food industry as a novel food. This study has been performed to approbate patatin determination method for evaluation of protein quality of potato genotypes, as well as evaluate patatin relative abundance (PRA) for breeding programmes to create in the future potato cultivars with higher value and potential to develop new products. The evaluation of patatin was performed in following steps– extraction proteins from potato, determination of patatin concentration and calculation of its relative abundance in proteins. Separation of patatin from potato tubers was made using extraction by SDS extraction buffer and determination of patatin in organically and conventionally (with differnt N suply) grown samples of 20 potato genotypes. The results of one-year study showed that patatin relative abundance of different cultivars varied from 1.65% to 50.2% and it was significantly different among genotypes. The nitrogen content of soil and maturity type of potato did not affect PRA significantly. Results provide impetus for further research.
Show more [+] Less [-]Fungi Causing Storage Rot of Apple Fruit in Integrated Pest Management System and their Sensitivity to Fungicides Full text
2016
Grantina-Ievina, Lelde
Apple fruit rot can be caused by several fungi. In Northern Europe, the most common storage rot, Bull’s eye rot, is caused by Neofabraea spp., bitter rot by Colletotrichum spp., brown rot by Monilinia fructigena, grey mould is caused by Botrytis cinerea and Fusarium rot by several Fusarium species. Blue mold decay caused by Penicillium expansum is an important disease in several European countries. Incidence of different causal agents may vary depending on cultivar, climate during growing season and agricultural practices. The main objective of the study was to obtain baseline information about apple rot-causing fungi, their incidence during fruit storage and to evaluate the fungicide sensitivity of most of isolated fungal species. The study was performed during the storage period of apples after the growth season of 2013. Rotten apples were sorted in the storage and part of them was brought to the laboratory in order to obtain fungal isolates. Fungi were identified according to the morphological characteristics and sequencing of the ITS1-5.8S-ITS2 region. During storage in February and March the total percentage of rotten apples in various cultivars varied from 3.6 to 58.9%. All post-harvest diseases described in Northern Europe were detected. In part of the storehouses apple rot caused by Cadophora luteo-olivacea was observed. Alternaria spp. and Cladosporium spp. were detected on few apples as secondary infection agents. Using the most often isolated fungal species, sensitivity tests were performed against five commonly used fungicides. In general, the sensitivity of tested fungi to the fungicides was high with exception of several Neofabraea and Alternaria isolates.
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