The hyper-androgen and huritism in face and body in women is important diseases. It can be used as a possible marker in patients with ovary polycystic syndrome (PCOS). This study was carried out to evaluate DHEA and Testosterone levels associated with huritism and PCOS was studied in serum samples from pregnant, and non-pregnant patients and controls attending clinic Specialized. The study included 38 samples divided into three groups: 21 patients subjects, 6 pregnant women, 15 non-pregnant women with PCOS syndrome, and 17 normal subjects as control of the same age group. The target  was selecting pregnant women due to changes in hormonal levels and physiological changes, as well as comparing them with non-pregnant women and control samples. The level of DHEAS was considerably higher in the median among pregnant women than in the control group and between non-pregnant and control groups without statistically significant differences between pregnant and non-pregnant values. Similarly, testosterone shows a statistically significant higher median among pregnant than control and also between non-pregnant and control groups without statistically significant difference between pregnant and non-pregnant values. In conclusion, this study detected a significant increase in testosterone and DHEA in non-pregnant women and then followed by pregnant women compared to the control, and age and the rise of these hormones are factors that cause the appearance of thick hair and thus cause polycystic ovary, which may lead to infertility.

The Borneo earless monitor lizard (Lanthanotus borneensis) is one of the endemic animals of Indonesia. In 2019, the IUCN listed L. borneensis as an ‘endangered’ species. The declining population of the L. borneensis is a major concern for conservation efforts. One of the efforts to conserve endangered wild animals is to know the gut microbiome profile and the relationship between the host and the bacterial community in the body, which is associated with an influence on health. This research aims to determine the gut microbiome profile of  animals with metagenomic analysis using Next-Generation Sequencing (NGS). This research was carried out by providing three feed types: earthworms, shrimp, and fish. Sample feces was collected by cloacal swabs and the DNA was sequenced using the Illumina NGS technique in the V4 16S rRNA region to examine the gut microbiome. The results of the metagenomic analysis showed differences in bacterial abundance in each feed treatment. The L. borneensis treated with earthworms and fish were dominated at the phylum level by Proteobacteria and Bacteriodota, while in shrimp, the phyla were Proteobacteria and Firmicutes. Index Shanon, Simpson, and the rarefaction curve also showed the highest bacterial diversity found in the fish treatment. This study can be used as a reference in designing suitable feed formulations for effective captive breeding for the conservation of L. borneensis

The present study emphasized the distribution and utilization of both wild and domesticated Musa spp. of the Nocte tribe from Tirap district, Arunachal Pradesh. An extensive field survey was conducted from July to October 2023. A total of 62 households were surveyed randomly with the help of a semi-constructed questionnaire and personal interview. About 69% of the informants were from the age group of 31-60 years. About 84% live in a nuclear family type. Maximum informants were male (74.19%) and were married (80.64%). Only 17.75% of the informants were illiterate and were farmers (46.77%). In this study, 13 wild Musa spp. were reported from Tirap district, and 4 Musa spp. were found to be cultivated by the tribe. Of these, 76.47% were wild, and the rest were domesticated. M. itinerens had a widespread distribution range and occurred in various habitats between 155 and 1,711 masl altitudes. The highest use percentage was found in the Edible use category with 33%, followed by Ceremonies and Rituals (19%), Commercial uses (12%), and both Other and Packing purposes (10% each). Among the plant parts, inflorescences had the highest usage with 30%, followed by leaf (24%), pseudo stem (21%), etc. Among all Musa spp., M. itinerens had the highest number of usages (14 uses), followed by M. nagensium (12 uses).

Soil microorganisms, especially diazotrophic bacteria, are vital for ecosystem sustainability, significantly contributing to nitrogen cycling and biodiversity conservation. Understanding the impact of different land-use practices on soil microorganisms, especially synthetic fertilizer addition, is essential for sustainable agriculture. This study compares soil properties, bacterial densities, and responses to fertilization in secondary forest soils and adjacent oil palm plantation soils located in PT Kerry Sawit Indonesia, Central Kalimantan. A greenhouse experiment was conducted using both secondary forest and oil palm plantation soils to evaluate the impacts of different synthetic fertilizers on nitrogen-fixing bacteria and soybean agronomic performance. Total soil bacteria and diazotrophic bacteria, essential for nitrogen fixation, were analyzed through microbiological assays and qPCR focusing on the nifH gene. Our findings show that oil palm plantation soils had higher levels of nitrogen, phosphate, and nitrate, while secondary forest soils had a higher abundance of diazotrophic bacteria. Furthermore, excessive nitrogen fertilization was found to reduce microbial density, negatively impacting plant sustainability, highlighting the importance of customized fertilizer management. The study highlights the intricate connections between land-use practices and microbial populations, providing valuable insights for balancing agricultural productivity with ecological sustainability.

The objective was to assess a range of stress indices to discern wheat genotypes resilient to drought stress, so forty-nine genotypes underwent scrutiny in both drought stress in rainfed conditions and non-stress settings (with supplementary irrigation), employing a 7 × 7 lattice layout with two replicates across years 2019 and 2020. The evaluation incorporated twenty stress indices anchored in yield under water stress (YS) and potential (YP) circumstances. Primary analysis indicated that eight indices (RDI, YSI, YI, K2STI, MRP, REI, RR and SSPI) did not give any new information, so they were eliminated in further analysis. Genotypes G33 (4234 kg ha-1) and G9 (2227 kg ha-1) were the best genotypes based on YP in 2019 and 2020, respectively. A positive association was observed between ATI and YP and between YS with DI and K1STI in the year 2019, while in the second year, such positive associations were not seen. We found some wheat genotypes G6, G9, G10 and G11 demonstrated high performance in both potential and rainfed conditions across two years, showing yield higher than 1,800 and 2,700 kg ha-1 for YS and YP, respectively, across both years. These genotypes were detected as the most tolerant genotypes by mean-based indices (TOL, HM, GMP, and MP) as well as SSI and ATI indices, so it can be concluded that these indices are more useful than other indices for identifying the most tolerant as well as the high yielding genotypes.

Burn injuries present a significant global health challenge, with the highest incidence rates reported in Southeast Asia, including Indonesia. Healing burn wounds is a complex and dynamic process involving various cellular and molecular mechanisms, prominently featuring the role of Vascular Endothelial Growth Factor (VEGF) in tissue regeneration and wound repair. VEGF is crucial for inducing and regulating angiogenesis and supplying oxygen and nutrients to the healing tissue. This study aims to evaluate the potential of pegagan (Centella asiatica) leaf extract cream 1%, 3%, and 5% daily for 14 days in enhancing VEGF expression and angiogenesis in second-degree burn wounds in mice (Mus musculus). This study investigates the application of C. asiatica extract cream on second-degree burn wounds in mice, comparing its effects on VEGF protein expression and angiogenesis to those of base cream and silver sulfadiazine cream, with outcomes evaluated using immunohistochemistry (IHC) and hematoxylin and eosin (HE) staining methods. Our findings suggest that C. asiatica extract cream promotes reduced burn wound size, significant upregulated VEGF expression, and enhanced angiogenesis in treating burn wounds compared to positive control, with a 5% dose having the best result. The study concludes that C. asiatica extract cream may effectively treat burn wound healing through enhancing  VEGF expression and angiogenesis.

Bst DNA polymerase possesses strand displacement activity, enabling isothermal DNA amplification without requiring a thermal cycler. This enzyme is utilized in the Loop-Mediated Isothermal Amplification (LAMP) method, which offers advantages in speed and simplicity over Polymerase Chain Reaction (PCR) method. The growing demand for Bst DNA polymerase highlights the need for cost-effective in-house production, as a commercial option is economically challenging. For that purpose, this study aims to construct and optimize the expression of Bst DNA polymerase from Geobacillus stearothermophilus in Escherichia coli. The expression constructs pET16b.BstHF vector was constructed using Gibson Assembly and expressed in E. coli BL21 (DE3). Optimal expression was achieved with 1 mM IPTG, induction at OD600 0.8 and 6-hour induction time. The purified enzyme was achieved with a protein yield of 2,175 mg/L culture and demonstrated effective polymerase activity for LAMP.

N-Methyl-N-Nitrosourea (MNU) is a compound that induces oxidative stress resulting in high levels of oxidants and damage to body cells. Red okra contains polyphenol and flavonoid active ingredients as antioxidants. This study aims to analyze the effect of ethanol extract of red okra pods (ROPE) on oxidant levels, antioxidant enzymes, and damage to rat kidney proximal tubule cells due to NMU. This study used 30 rats divided into six treatment groups, namely CN (normal), N (negative control; MNU 50 mg/kg BW), P (positive control; MNU and MTX 50 mg/kg BW), T1 (MNU and ROPE 50 mg/kg BW), T2 (MNU and ROPE 100 mg/kg BW), and T3 (MNU and ROPE 200 mg/kg BW). The treatment was carried out on all groups after eight weeks. The results indicate that malondialdehyde (MDA) and nitrogen oxide (NO) decrease with the ROPE treatment. The glutathione reductase (GSH) activity as an antioxidant enzyme increased T1 and T2, while glutathione peroxidase (GPx) showed an increase in T2 and T3. Furthermore, the biochemical marker of the rat kidney showed lower blood urea nitrogen (BUN) and creatinine (Cre) levels in all treatment groups. Then, the repair of damaged proximal tubule cells showed an increase in normal cells and lower swollen cells; however, there was a degradation in necrotic cells in T2 and T3. It can be indicated that the ROPE can act as an antioxidant that can reduce MDA and NO levels, increase GSH and GPx levels, and reduce damage to proximal renal tubule cells due to MNU.

Basal stem rot disease caused by Ganoderma boninense is a major problem for oil palm cultivation. The research was conducted to obtain biocontrol agents from rhizosphere of oil palm to control the disease as part of sustainable pest management in oil palm plantation. Rhizosphere bacteria were isolated from rhizospheres of healthy oil palm trees. Isolation of bacteria was done using serial dilution method. The isolated bacteria were then tested for its antifungal activity against G. boninense in vitro using dual culture assay. The ability of the bacteria to produce antifungal compound was also determined by culturing the bacteria on ISP2 liquid media. Once the bacterial cells were removed, the crude metabolites were then tested against G. boninense using agar well diffusion and toothpick colonization. The result showed that several isolates demonstrated strong antifungal activity against G. boninense. Some isolates were also able to degrade chitin and to solubilize phosphate. Furthermore, the crude metabolites produced by the rhizosphere bacteria demonstrated the ability to inhibit the growth of G. boninense in the agar diffusion method. Colonization of the G. boninense on toothpick following soaking in the crude metabolites was also inhibited. The isolated rhizosphere bacteria (BARK7 and BARK15 in which identified as Burkholderia sp.) showed promising ability to be developed as biocontrol agent for basal stem rot disease of oil palm.

Foxtail millet hybrid development encounters challenges due to its self-pollinating nature, small florets, and tool availability for confirming true hybrid. Our research aimed to assess flowering phenology and explore artificial hybridization employing SiDREB2 based-SNAP marker. The research consisted of three experiments. The first experiment focused on the panicle phenology of foxtail millet, while the second experiment focused on floret phenology and reproductive organs. The artificial hybridization successfulness was validated with SNAP markers based on the SiDREB2 gene in the third experiment. Observations on two genotypes (ICERI-5 and ICERI-6) revealed panicle initiation at 49–69 days after planting (DAP), with anthesis occurring 6 days after panicle emergence (DAPE). Florets exhibited the formation of reproductive organs at 3 DAPE, delineating distinct stages of pollen development leading up to anthesis. Our findings emphasized the significance of implementing male-sterile induction treatment at 4 DAPE to regulate pollen development for successful hybridization by 6 DAPE. The hybrid evaluation revealed varied germination rates and confirmed hybrid percentages across different crossing series, validated by SiDREB2-based SNAP marker. ICERI-5×Botok-4 exhibited high germination but a low confirmed hybrid percentage, while Botok-4×ICERI-5 and Botok-10×ICERI-6 showed the opposite trend. Reciprocal crossings showed unilateral incompatibility in confirmed hybrid percentage.