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Acceptable microorganisms concentration in a semen sample for in vitro embryo production Full text
2006
D'Angelo, Magali(Instituto Biológico de São Paulo Centro de Sanidade Animal Laboratório de Biologia Celular) | Pavão, Danielle L.(Instituto Biológico de São Paulo Centro de Sanidade Animal Laboratório de Biologia Celular) | Melo, Gisele Martins(Instituto Biológico de São Paulo Centro de Sanidade Animal Laboratório de Biologia Celular) | Rojas, Natali(Instituto Biológico de São Paulo Centro de Sanidade Animal Laboratório de Biologia Celular) | Souza, Rosimeire Joaquim(Instituto Biológico de São Paulo Centro de Sanidade Animal Laboratório de Biologia Celular) | Athayde, Christiani(Instituto Biológico de São Paulo Centro de Sanidade Animal Laboratório de Biologia Celular) | Galuppo, Andrea Giannotti(Instituto Biológico de São Paulo Centro de Sanidade Animal Laboratório de Biologia Celular) | Pinheiro, Eliana Scarcelli(Instituto Biológico de São Paulo Centro de Sanidade Animal Laboratório de Doenças Bacterianas da Reprodução)
The aim of this study was to report that the acceptable concentration of microorganisms in a semen sample for insemination may not be safe for an in vitro fertilization procedure. It seems that the semen sample should be completely germ-free, because of the excellent microorganism proliferation condition promoted by the in vitro environment. | O objetivo foi relatar que a concentração de microrganismos aceitável para uma amostra de sêmen utilizada para inseminação pode não ser segura para a realização de fertilização in vitro. Aparentemente a amostra deve ser isenta da presença de contaminantes, pois a condição in vitro promove ambiente favorável para seu crescimento.
Show more [+] Less [-]Effects of Atrazine on Soil Microorganisms Full text
2006
Ljiljana Radivojević | Ljiljana Šantrić | Radmila Stanković-Kalezić
Effects of the herbicide atrazine on soil microorganisms was investigated. Trials were set up in laboratory, on a clay loam soil. Atrazine was applied at 8.0, 40.0 and 80.0 mg/kg soil rates. The abundance of total microorganisms, fungi, actinomycetes, cellulolytic microorganisms and amino-heterotrophs was recorded. Soil samples were collected 1, 7, 14, 21, 30 and 60 days after atrazine treatment for microbiological analyses.The results showed that the intensity of atrazine effect on soil microorganisms depended on treatment rate, exposure time and group of microorganisms. Atrazine had an inhibiting effect on cellulolytic microorganisms and amino-heterotrophs. Initially, it inhibited fungiand actinomycetes but its effect turned into a stimulating one once a population recovered. Atrazine had a stimulating effect on total abundance of microorganisms.
Show more [+] Less [-]Effects of atrazine on soil microorganisms
2006
Radivojevic, Lj.(Institut za pesticide i zastitu zivotne sredine, Beograd (Serbia)) | Santric, Lj.(Institut za pesticide i zastitu zivotne sredine, Beograd (Serbia)) | Stankovic-Kalezic, R.(Institut za pesticide i zastitu zivotne sredine, Beograd (Serbia))
Effects of the herbicide atrazine on soil microorganisms was investigated. Trials were set up in laboratory, on a clay loam soil. Atrazine was applied at 8.0, 40.0 and 80.0 mg/kg soil rates. The abundance of total microorganisms, fungi, actinomycetes, cellulolytic microorganisms and amino-heterotrophs was recorded. Soil samples were collected 1, 7, 14, 21, 30 and 60 days after atrazine treatment for microbiological analyses. The results showed that the intensity of atrazine effect on soil microorganisms depended on treatment rate, exposure time and group of microorganisms. Atrazine had an inhibiting effect on cellulolytic microorganisms and amino-heterotrophs. Initially, it inhibited fungi, and actinomycetes but its effect turned into a stimulating one once a population recovered. Atrazine had a stimulating effect on total abundance of microorganisms.
Show more [+] Less [-]Phylogeny of Acetate-Utilizing Microorganisms in Soils along a Nutrient Gradient in the Florida Everglades Full text
2006
Chauhan, Ashvini | Ogram, Andrew
The consumption of acetate in soils taken from a nutrient gradient in the northern Florida Everglades was studied by stable isotope probing. Bacterial and archaeal 16S rRNA gene clone libraries from eutrophic and oligotrophic soil microcosms strongly suggest that a significant amount of acetate is consumed by syntrophic acetate oxidation in nutrient-enriched soil.
Show more [+] Less [-]Simultaneous P-solubilizing and biocontrol activity of microorganisms: potentials and future trends Full text
2006
Vassilev, Nikolay | Vasileva, Marii︠a︡ | Nikolaeva, Iana
Phosphate (P)-solubilizing microorganisms as a group form an important part of the microorganisms, which benefit plant growth and development. Growth promotion and increased uptake of phosphate are not the only mechanisms by which these microorganisms exert a positive effect on plants. Microbially mediated solubilization of insoluble phosphates through release of organic acids is often combined with production of other metabolites, which take part in biological control against soilborne phytopathogens. In vitro studies show the potential of P-solubilizing microorganisms for the simultaneous synthesis and release of pathogen-suppressing metabolites, mainly siderophores, phytohormones, and lytic enzymes. Further trends in this field are discussed, suggesting a number of biotechnological approaches through physiological and biochemical studies using various microorganisms.
Show more [+] Less [-]Inactivation of Airborne E. coli and B. subtilis Bioaerosols Utilizing Thermal Energy
2006
Lee, Y.H. (Carnegie Mellon University, Pittsburgh, PA, USA) | Lee, B.U. (Konkuk University, Seoul, Republic of Korea), E-mail: leebu@konkuk.ac.kr
Airborne microorganisms, which are currently termed bioaerosols, have received attention owing to the harmful effects they have on human health. As the concern over airborne microorganisms grows, there also grows an urgent need to study and develop efficient methods for controlling them. In this study, thermal energy using a thermal tube was tested as a control method, mainly against airborne E. coli. For a comparison, B. subtilis var. niger spores were utilized in the experimentation. It was found that the widely known inactivation conditions for microorganisms were not adequate against airborne microorganisms.
Show more [+] Less [-]Presence of thermoresistant microorganisms in semihard cheese during storage
2006
Oljacic, E.M.(JPS Zavod za mlekarstvo, Novi Beograd (Serbia)) | Kasalica, A.J.(JPS Zavod za mlekarstvo, Novi Beograd (Serbia))
In this paper the presence of thermoresistant microorganisms and their lipolytic and proteolytic activity, such as the ability to grow in storage temperature up to the expire date has been researched. Samples of cheeses were taken directly from a dairy after production and six-months storage at the temperature of 7 deg C. All researched samples were microbiologically correct, according to the legislation. The results of the research show the presence of thermoresistant microorganisms lipolytic, as well as proteolytic. Isolated microorganisms belonging to genus Bacillus and Micrococcus and showed psychrotrophic character, that reflected in the increase of total count of thermoresistant microorganisms at the end of storage. Presence of these microorganisms influenced shelf-life and sensory characteristics of cheese.
Show more [+] Less [-]Microbial diversity on paintings and engravings in Doña Trinidad Cave (Ardales, Spain) Full text
2006
Stomeo, Francesca | Laiz Trobajo, L. | González Grau, Juan Miguel | Sáiz-Jiménez, Cesáreo
International Conference, Madrid, 21-24 June, 2006. 8th Thematic Network on Cultural and Historic Heritage Scientific Meeting Stone, Weathering and Atmospheric Pollution Network Scientific Meeting. | The Cave of Doña Trinidad (Ardales, Malaga, Spain) contains numerous paintings and engravings dated back to 20,000 years. In this study, we study the microorganisms thriving on these paintings and engravings using molecular and culturing approaches. Molecular methods were based on the detection of microorganisms from DNA and RNA extracted directly from minute samples collected at the cave and did not require the culture of these microorganisms. Culturing methods required the growth of these microorganisms on previously determined culture media. Media used in this study were appropriated for the growth of aerobic and anaerobic heterotrophs. Results showed the presence of a large diversity of microorganisms detected from both molecular and culturing methods and a large proportion of them was presenting highest homology to so far uncultured mi croorganisms. Cultures allowed a detailed characterization of the function and capabilities of these microorganisms. The diversity found in this cave will be disc ussed focusing on the potential function of these microorganisms in the studied environment. | The authors acnowledge to the European Commission, for a Marie Curie Action, project MEST-CT-2004-513915. | The authors acknowledge funding the Junta de Andalucía, Consejería de Cultura to study the Cave of Doña Trinidad in Ardales, to the staff of the Museum of Ardales for facilities, | Peer reviewed
Show more [+] Less [-]The effect of irrigation on the occurrence of different microorganisms groups in the soil of apple-trees orchard after replanted
2006
Styla, K. | Wolna-Maruwka, A.,Akademia Rolnicza, Poznan (Poland). Katedra Mikrobiologii Rolnej
The effect of differentiated irrigation on the occurrence of microorganisms (bacteria, fungi, actinomycetes, Azotobacter, proteolytic bacteria, phosphate solubilizing bacteria) in the soil of the apple-trees orchard was investigated. The numbers of different microorganisms in the soil were diversified in depend on level irrigation and sampling date. A significantly correlation was observed between the numbers of microorganisms and pH, the contents total of organic carbon in the apple-trees orchard soil after replantation
Show more [+] Less [-]Integrating metabolomics into a systems biology framework to exploit metabolic complexity: strategies and applications in microorganisms Full text
2006
Wang, Qing-zhao | Wu, Chan-yuan | Chen, Tao | Chen, Xun | Zhao, Xue-ming
As an important functional genomic tool, metabolomics has been illustrated in detail in recent years, especially in plant science. However, the microbial category also has the potential to benefit from integration of metabolomics into system frameworks. In this article, we first examine the concepts and brief history of metabolomics. Next, we summarize metabolomic research processes and analytical platforms in strain improvements. The application cases of metabolomics in microorganisms answer what the metabolomics can do in strain improvements. The position of metabolomics in this systems biology framework and the real cases of integrating metabolomics into a system framework to explore the microbial metabolic complexity are also illustrated in this paper.
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