Xylanase and cellulase production by several molds using corn (Zea mays. L.) cobs as carbon substrate
1984
Matanguihan, R.M. | San Luis, M.M. | Rosario, E.J. del
The top three xylanase producers were found to be A. niger NRRL 337, A. niger NRRL 330 and T. viride QM 9123 A. niger NRRL 337 gave the highest xylanolytic activity, namely, 6.26 IU/ml volumetric activity and 7.36 IU/mg protein specific activity after 48 hours of culture. Trichoderma viride QM 9123, A. niger NRRL 330 and Penicillium sp. 86 were evaluated for cellulase production using corn cobs as carbon source. The three microorganisms showed maximum filter paper volumetric activity (FPVA) after 61 hoursof shake flask culture. The highest activity obtained during the screening experiments was 0.119 IU/ml from Penicillium sp. 86. Production of cellulase by Penicillium sp. 86 in shake flask culture containing 2% ground corn cobs (40 mesh size) gave a FPVA of 0.14 IU/ml after 72 hours of batch culture. Ground corn cobs at 2.5% soilds level showed 16% saccharification by Penicillium sp. 86 cellulase after incubation at 50 deg C, pH 4.8 for six hours. Alkali-treatment enhanced saccharification to 21.4% at 2.5% solids level
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