Studies on enzymes of the higher fungi of Korea, 2; identification of cellulolytic enzyme in Lenzites betulina
1986
Park, W.H. (Kyonggi Technical Open Univ., Seoul (Korea R.). Dept. of Environmental Engineering) | Kim, T.H. | Ro, I.H. (Sookmyung Women's Univ., Seoul (Korea R.). Coll. of Pharmacy)
Cellulosic substance which plays an important role in carbon cycle was most abundant in the nature world. Some higher fungi were able to digest cellulose directly to satisfy their carbohydrate requirement. Then, in order to investigate the enzymatic components of Lenzites betulina (Fr.) being wood rot, that fungus was collected in Kwangneung area. The carpophore of the fungus was smahed with cool distilled water, extracted and salted out by ammonium sulfate. And then the precipitate was purified by dialysing with visking tube and dissolved with pH 7.8 ammonia water, and the extract was filtrated. The fraction of filtrate was obtained as light brown powder after lyophilization, and determined cellulolytic activity
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