Response of Sesbania rostrata to different methods of inoculation and soil types [Philippines]
1986
Marqueses, E.P. | Furoc, R.E. | Dizon, M.A. (International Rice Research Inst., Los Banos, Laguna (Philippines))
A pot experiment was conducted in SEARCA [Southeast Asian Regional Center for Graduate Study and Research in Agriculture, College, Laguna, Philippines]-BioAssay Laboratory using Sesbania rostrata inoculated with an African strain (ORS 571) of Rhizobium sp. Seed inoculation, stem inoculation (sprayed) and a combination of seed and stem inoculation methods were applied. Uninoculated control pots were included to check inoculation method effects. Nodulation total day matter (TDM), total nitrogen (N) content (micro-Kjeldahl), N2 fixation (acetylene reduction assay), and N uptake at 15, 30, and 45 days after emergence growth period were determined. The soils used were from IRRI [International Rice Research Institute] (Block O), Cavinti (Laguna) and Guimba (Nueva Ecija, Philippines). The pH of the Cavinti soil was 4.8, the IRRI soil 6.2 and the Guimba soil 6.4. Nodulation (both root and stem), nodule dry weight, TDM total N content, N2 fixed and N uptake was lowest in the Cavinti soil. Inoculation methods used in this type of soil were not significant. IRRI soil and Guimba soil gave the highest nodule counts. Nodulation, TDM, total N content, N2 fixation, and N uptake were high at IRRI and Guimba soils. Among the inoculation methods, seed inoculation and combination of seed and stem inoculation gave highly significant response to the parameters used compared to stem inoculation and uninoculated control pots.
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